Dynamics of internal isoprenoid metabolites in young Picea abies (Norway spruce) shoots during drought stress conditions in springtime.

Currently, large areas of Picea abies (Norway spruce) stands in Europe are increasingly affected by drought and heat waves. Moreover, early spring drought has occurred with much higher frequency. Our work focuses on physiological changes induced by drought in four-year-old spruce seedlings during shoot elongation. We investigated drought effect on photosynthetic rate, concentration of abscisic acid and its metabolites, amount and composition of monoterpenes in needles of seedlings from five different provenances (altitude range 550-1280 m above sea level) in Western Carpathians. Spruce seedlings subjected to one-month drought stress of moderate intensity (about 50% of soil water content at the end of experiment) showed significant reduction of CO2 uptake and increased concentration of ABA related to untreated controls. Induced drought affected needle monoterpene content and composition. Observed changes in drought-induced physiological parameters were influenced by seedling provenance. The provenance from 920 m above sea level showed the greatest sensitivity to drought with significantly highest ABA content and, at the same time, a clear decline of CO2 uptake and amounts of total monoterpenes. Our results indicating intra-specific provenance-related variability in physiological response of spruce seedlings to drought may provide a basis for improved reforestation strategies in drought risk areas.

Anti-attractant activity of (+)-trans-4-thujanol for Eurasian spruce bark beetle Ips typographus: Novel potency for females.

BACKGROUND: Eurasian spruce bark beetles (Ips typographus) use both attractant and anti-attractant semiochemicals to find suitable mature host trees. Trans-4-thujanol is abundant in young, unsuitable spruce trees. Electrophysiological studies have demonstrated its high activity levels, but field data are lacking. RESULTS: Enantioselective GC-MS analysis showed that only (1R,4S)-(+)-trans-4-thujanol was present in Norway spruce bark volatiles. In a factorial design field-trapping experiment, trans-4-thujanol alone was not attractive to Ips typographus. Traps baited with I. typographus' aggregation pheromone and trans-4-thujanol or the known anti-attractant 1,8-cineole caught fewer beetles than those baited with the aggregation pheromone alone. Catches for trans-4-thujanol and 1,8-cineole were dose-dependent. Intermediate doses of trans-4-thujanol and 1,8-cineole had a similar effect. Surprisingly, in contrast to 1,8-cineole and other known Ips anti-attractants, which all inhibit males more strongly than females, the addition of trans-4-thujanol to the aggregation pheromone reduced the attraction of females more. CONCLUSION: The Norway spruce volatile (+)-trans-4-thujanol is a novel I. typographus anti-attractant with potency comparable to the known anti-attractants 1,8-cineole and verbenone, and is more effective for females than for males. Incorporating (+)-trans-4-thujanol into anti-attractant lures could improve protection of trees from mass attack by I. typographus.

The 2018 European heatwave led to stem dehydration but not to consistent growth reductions in forests.

Heatwaves exert disproportionately strong and sometimes irreversible impacts on forest ecosystems. These impacts remain poorly understood at the tree and species level and across large spatial scales. Here, we investigate the effects of the record-breaking 2018 European heatwave on tree growth and tree water status using a collection of high-temporal resolution dendrometer data from 21 species across 53 sites. Relative to the two preceding years, annual stem growth was not consistently reduced by the 2018 heatwave but stems experienced twice the temporary shrinkage due to depletion of water reserves. Conifer species were less capable of rehydrating overnight than broadleaves across gradients of soil and atmospheric drought, suggesting less resilience toward transient stress. In particular, Norway spruce and Scots pine experienced extensive stem dehydration. Our high-resolution dendrometer network was suitable to disentangle the effects of a severe heatwave on tree growth and desiccation at large-spatial scales in situ, and provided insights on which species may be more vulnerable to climate extremes.

Peripheral modulation of pheromone response by inhibitory host compound in a beetle.

We identified several compounds, by gas chromatographic-electroantennographic detection (GC-EAD), that were antennally active in the bark beetle Ips typographus and also abundant in beetle-attacked spruce trees. One of them, 1,8-cineole (Ci), strongly inhibited the attraction to pheromone in the field. Single-sensillum recordings (SSRs) previously showed olfactory receptor neurons (ORNs) on I. typographus antennae selectively responding to Ci. All Ci neurons were found within sensilla co-inhabited by a pheromone neuron responding to cis-verbenol (cV); however, in other sensilla, the cV neuron was paired with a neuron not responding to any test odorant. We hypothesized that the colocalization of ORNs had a functional and ecological relevance. We show by SSR that Ci inhibited spontaneous activity of the cV neuron only in sensilla in which the Ci neuron was also present. Using mixtures of cV and Ci, we further show that responses to low doses (1-10 ng) of cV were significantly reduced when the colocalized Ci neuron simultaneously responded to high doses (1-10 µg) of Ci. This indicated that the response of the Ci neuron, rather than ligand-receptor interactions in the cV neuron, caused the inhibition. Moreover, cV neurons paired with Ci neurons were more sensitive to cV alone than the ones paired with the non-responding ORN. Our observations question the traditional view that ORNs within a sensillum function as independent units. The colocalization of ORNs might sharpen adaptive responses to blends of semiochemicals with different ecological significance in the olfactory landscape.

Feeding response to host and nonhost compounds by males and females of the spruce bark beetle Ips typographus in a tunneling microassay.

Research on host selection by bark and wood boring insects has concentrated on flight orientation behavior. Less is known of the factors that govern the steps successive to host landing. Here, we discuss chemical factors involved in host acceptance by bark beetles and a new microassay. Adult males and females of Ips typographus were offered an artificial diet treated with various concentrations of different plant-derived compounds (host terpenes and nonhost compounds) in a no-choice mode. Beetles were tested individually in a glass tube for 4 hr, and the length of feeding was measured and compared to a control (diet with only solvent). The first effect was diet rejection, especially when nonhost compounds were tested at high concentrations. Most compounds reduced feeding, in proportion to concentration. Females fed more readily than males after addition of both host and nonhost compounds. Diet removal was significantly affected by all the tested factors (sex, compound, dose) as well as by their interactions. With increased concentrations, males were more responsive than females to antifeedants, as all compounds (except juglone) showed clear sex differences of diet consumption. 3-Octanol, 1-hexanol, and a Green Leaf Volatile (GLV)-blend (three C6 alcohols) showed the strongest antifeedant effects, which started at a low dose (0.1%) and had a low Effective Dose 50 (ED50, 0.3-1%). In contrast, host monoterpenes, limonene and alpha-pinene, inhibited feeding at high doses (10-30%) only, with ED50 > 10%. The highest Antifeedant Indexes were shown by verbenone, carvone, and 1-hexanol (AFI = 0.90-1.00). Both host and nonhost compounds inhibited feeding at some concentration. No significant stimulation of feeding by any host compound at concentrations reported in the literature as optimal were found, with the possible exception of alpha-pinene at low concentrations in females.