Regional evaluation of glyphosate pollution in the minor irrigation network.

Due to its low cost, its ease of use and to the "mild action" declared for long time by the Control and Approval Agencies towards it, the herbicide Glyphosate, is one of the currently best-selling and most-used agricultural products worldwide. In this work, we evaluated the presence and spread of Glyphosate in the Po River Basin (Northern Italy), one of the regions with the most intensified agriculture in Europe and where, by now for decades, a strong and general loss of aquatic biodiversity is observed. In order to carry out a more precise study of the real presence of this herbicide in the waters, samples were collected from the minor water network for two consecutive years, starting in 2022, at an interval time coinciding with those of the spring and summer crop treatments. In contrast to the sampling strategies generally adopted by Environmental Protection Agencies, a more focused sampling strategy was adopted to highlight the possible high concentrations in minor watercourses in direct contact with cultivated fields. Finally, we investigated the possible consequences that the higher amounts of Glyphosate found in our monitoring activities can have on stress reactions in plant (Groenlandia densa) and animal (Daphnia magna) In all the monitoring campaigns we detected exceeding European Environmental Quality Standard - EQS limits (0.1 µg/L) values. Furthermore, in some intensively agricultural areas, concentrations reached hundreds of µg/L, with the highest peaks during spring. In G. densa and D. magna, the exposition to increasing doses of herbicide showed a clear response linked to metabolic stress. Overall, our results highlight how, after several decades of its use, the Glyphosate use efficiency is still too low, leading to economic losses for the farm and to strong impacts on ecosystem health. Current EU policy indications call for an agroecological approach necessary to find alternatives to chemical weed control, which farms can develop in different contexts in order to achieve the sustainability goals set by the Farm to Fork strategy.

A pipeline for monitoring water pollution: The example of heavy metals in Lombardy waters.

Time-dependent geolocalized analysis of pollution data allows to better understand their dynamics over time and could suggest strategies to restore a good ecological status of contaminated area. This research analyzes concentrations of pollutants in surface waters and groundwater monitored by the Regional Environment Protection Agency of Lombardy from 2017 to 2020. Lombardy is one of the richest and populous region of Europe, providing an interesting example of the impact of environmental pollutants due to anthropogenic and industrial activities, not only for Italy but also for all Europe. Results show that groundwater displays more sites with heavy metals above the legal limit with respect to surface waters, including As, Ni, Cr and Zn. Furthermore, the spatio-temporal analysis of the data clearly shows that the introduction of more restrictive laws is a proper policy to improve the ecological status of the water.

The environmental impacts of different organic rice management in Italy considering different productive scenarios.

Rice cultivation has a key role in food security worldwide; on the other hand, it has a high potential impact on the environment and human health, mainly due to the extensive pesticides use and greenhouse gas emissions caused by flooded cultivation. In Italy, the rice sector based mainly on high-input monoculture. The transition toward organic agriculture can improve the environmental performance of rice farming according to the actual European sustainable food production strategy. Through LCA methodology, the study aims to evaluate the variability of the environmental impacts and the mitigation potential of four management strategies suitable for organic rice production in North Italy and two production potential levels observed during three-year monitoring on 10 farms in the study area. The LCA analysis includes the wide range of agronomic realities that characterise this farming system, assessing the variation in environmental performance by exploring eight plausible and possible scenarios for organic rice. Results suggest a considerable potential of organic rice production to mitigate its impact on natural resources, depending on the chosen agricultural practices. In particular, six LCA indicators showed a potential of reduction over 40 %, shifting from the worst-performing management to the better one. Finally, the large variability of climate change impacts assessed, both in this study and in literature, is due to the corresponding existing large variability in terms of yield and available patterns of agricultural practices. Today the farmers could reach acceptable yield values thanks to more efficient management than in the past. The acknowledgement for that performances relates to the development of the farmers' know-how and to the productive improvement connected to the long-term processes which characterise the organic systems (e.g. generation of soil fertility based on biological fertility and stable humus components; lowering of weeds pressure through the gradual introduction of other crops in rotation).

High-amylose and Tongil type Korean rice varieties: physical properties, cooking behaviour and starch digestibility.

The National Institute of Crop Science, Rural Development Administration (RDA) of Korea is presently developing new rice varieties suitable for producing Western rice-based foods, such as risotto, a well-known Italian-style product. The study considered different milled rice from five Tongil-type and six Japonica-type varieties. Besides the biometric properties, cooking behaviour, starch properties, and in vitro digestibility of Korean rice samples were compared with those of the 'Carnaroli' Italian variety. The physicochemical traits of the Korean varieties extended over a vast range; the amylose content stood out (from 13.0 to 41.7%), influencing the hardness and stickiness of cooked samples, and their starch digestibility. Although none of the Korean varieties seemed to guarantee cooking performances for risotto similar to the 'Carnaroli' one, 'Saemimyeon' and 'Shingil' cvs were judged the best for this purpose up-to-now.

Synergistic effects of contaminants in Lombardy waters.

Quantifying synergistic environmental effects in water contamination is still an open issue. Here, we have analyzed geolocalized data of pollutants recorded in 2018 in surface and groundwater of Lombardy, one of the areas with the highest agricultural production rates, not only in Italy, but also in Europe. Both herbicides and insecticides are present at concentration levels above the legal limit, mainly in surface waters. Geolocalized analysis allows us to identify interesting areas particularly affected by a combination of multiple pesticides. We thus investigated possible synergistic effects of these compounds on the environment, using the alga C. reinhardtii as a biosensor. Our results show that exposure for 7 days to four compounds, that we found present together at high concentration in surface waters, was able to induce a stress in the algae, as indicated by the presence of palmelloids. Our work results in a pipeline that could easily be exported to monitor other territories in Italy and abroad.

Correction to: Different phytotoxic effect of Lolium multiflorum Lam. leaves against Echinochloa oryzoides (Ard.) Fritsch and Oryza sativa L.

In the title, it should be Oryza instead of Oriza.

Different phytotoxic effect of Lolium multiflorum Lam. leaves against Echinochloa oryzoides (Ard.) Fritsch and Oryza sativa L.

Rice cultivation, particularly prone to weed issues, requires practices able to effectively control them, however reducing the use of herbicides, responsible for damage to human health and ecosystem sustainability. Alternative strategies for weed management can be based on plant-plant interaction phenomena. In this context, a group of organic farmers has developed a pragmatic approach for weed containment using Lolium multiflorum Lam. as a cover crop before rice. The present study aimed to confirm the farmer field observations reporting a preferential inhibitory effect of L. multiflorum on Echinochloa oryzoides (Ard.) Fritsch, one of the most yield-damaging rice weed, compared with Oryza sativa L. The study showed that L. multiflorum was able to significantly reduce the seed germination of E. oryzoides. It was found to be more susceptible than O. sativa both to the effect of the aqueous extract and powder of L. multiflorum leaves (23-79% vs. 3-57% and 26-100% vs. 23-31%, respectively). In addition, the leaf extract was able to affect E. oryzoides growth starting from 20% concentration both in relation to the root and shoot length while O. sativa exhibited differences compared with the control only under the influence of extract 50%. The L. multiflorum leaf characterization by NMR and UPLC-HR-MS analyses led to the identification of 35 compounds including several polyphenols, glycosyl flavonoids and glycosyl terpenoids, as well as different amino acids and organic acids. Some of them (e.g. protocatechuic and gallic acids) are already known as allelochemicals confirming that L. multiflorum is a source of plant growth inhibitors.

Potential Role of Lolium multiflorum Lam. in the Management of Rice Weeds.

The phytotoxic relationships between crops and weeds can cover a role in weed management, reducing the use of chemical herbicides. Starting from the organic farmers' experience, the study aimed to define the inhibitory action of Lolium multiflorum Lam., used as a cover crop before rice sowing, against Echinochloa oryzoides (Ard.) Fritsch, one of the main rice weeds. In vitro 7-day assays were carried out in Petri dishes to compare the effect of different L. multiflorum Lam. parts, in the form of aqueous extract or powder, on the seed germination and seedling growth of Oryza sativa L. and E. oryzoides and to verify the hypothesis of a higher susceptibility of the weed. The total polyphenolic content, as the potential source of allelochemicals, in the L. multiflorum parts was measured. The results showed that both species suffer the phytotoxic action of L. multiflorum, but a more marked effect against E. oryzoides was recorded. In according with the polyphenol quantities, stem and inflorescence extracts showed the more significant species-specific inhibition. In all assays, the weed showed a stronger reduction in the root length and seedling vigor index, and, in some cases, also in the germination percentage and shoot length compared to rice.

A Storable Mediatorless Electrochemical Biosensor for Herbicide Detection.

A novel mediatorless photo-bioelectrochemical sensor operated with a biofilm of the cyanobacterium Synechocystis PCC6803 wt. for herbicide detection with long term stability (>20 days) was successfully developed and tested. Photoanodic current generation was obtained in the absence of artificial mediators. The inhibitory effect on photocurrent of three commonly used herbicides (i.e., atrazine, diuron, and paraquat) was used as a means of measuring their concentrations in aqueous solution. The injection of atrazine and diuron into the algal medium caused an immediate photocurrent drop due to the inhibition of photosynthetic electron transport. The detected concentrations were suitable for environmental analysis, as revealed by a comparison with the freshwater quality benchmarks set by the Environmental Protection Agency of the United States (US EPA). In contrast, paraquat caused an initial increase (~2 h) of the photocurrent effect of about 200%, as this compound can act as a redox mediator between the cells and the anode. A relatively long-term stability of the biosensor was demonstrated, by keeping anodes colonized with cyanobacterial biofilm in the dark at 4 °C. After 22 days of storage, the performance in terms of the photocurrent was comparable with the freshly prepared biosensor. This result was confirmed by the measurement of chlorophyll content, which demonstrated preservation of the cyanobacterial biofilm. The capacity of this biosensor to recover after a cold season or other prolonged environmental stresses could be a key advantage in field applications, such as in water bodies and agriculture. This study is a step forward in the biotechnological development and implementation of storable mediatorless electrochemical biosensors for herbicide detection.

Thermotolerant isolates of Beauveria bassiana as potential control agent of insect pest in subtropical climates.

The use of Beauveria bassiana in biological control of agricultural pests is mainly hampered by environmental factors, such as elevated temperatures and low humidity. These limitations, further amplified in a global warming scenario, could nullify biological control strategies based on this fungus. The identification of thermotolerant B. bassiana isolates represents a possible strategy to overcome this problem. In this study, in order to maximize the probability in the isolation of thermotolerant B. bassiana, soil samples and infected insects were collected in warm areas of Syria. The obtained fungal isolates were tested for different biological parameters (i.e., growth rate, sporulation and spore germination) at growing temperatures ranging from 20°C to 35°C. Among these isolates (eight from insects and 11 from soil samples), the five with the highest growth rate, spore production and germination at 30°C were tested for their entomopathogenicity through in vivo assays on Ephestia kuehniella larvae. Insect mortality induced by the five isolates ranged from 31% to 100%. Two isolates, one from Phyllognathus excavatus and one from soil, caused 50% of the larval mortality in less than four days, reaching values exceeding 92% in ten days. These two isolates were molecularly identified as B. bassiana sensu stricto by using three markers (i.e., ITS, Bloc and EF1-α). Considering these promising results, further studies are ongoing, testing their efficiency in field conditions as control agents for agricultural insect pests in Mediterranean and Subtropical regions.

Evaluation of anaerobic degradation, biogas and digestate production of cereal silages using nylon-bags.

In this study, the degradation efficiency and the biogas and digestate production during anaerobic digestion were evaluated for the cereal silages most used to feed biogas plants. To this purpose, silages of: maize from the whole plant, maize from the ear, triticale and wheat were digested, inside of nylon bags, in laboratory scale digesters, for 75days. Overall, the test involved 288 nylon bags. After 75days of digestion, the maize ear silage shows the highest degradation efficiency (about 98%) while wheat silage the lowest (about 83%). The biogas production ranges from 438 to 852Nm(3)/t of dry matter for wheat and ear maize silage, respectively. For all the cereal silages, the degradation as well as the biogas production are faster at the beginning of the digestion time. Digestate mass, expressed as percentage of the fresh matter, ranges from 38% to 84% for wheat and maize ear silage, respectively.

Environmental profile of paddy rice cultivation with different straw management.

Italy is the most important European country in terms of paddy rice production. North Italian districts such as Vercelli, Pavia, Novara, and Milano are known as some of the world's most advanced rice cultivation sites. In 2013 Italian rice cultivation represented about 50% of all European rice production by area, and paddy fields extended for over 216,000 ha. Cultivation of rice involves different agricultural activities which have environmental impacts mainly due to fossil fuels and agrochemical requirements as well as the methane emission associated with the fermentation of organic material in the flooded rice fields. In order to assess the environmental consequences of rice production in the District of Vercelli, the cultivation practices most frequently carried out were inventoried and evaluated. The general approach of this study was not only to gather the inventory data for rice production and quantify their environmental impacts, but also to identify the key environmental factors where special attention must be paid. Life Cycle Assessment methodology was applied in this study from a cradle-to-farm gate perspective. The environmental profile was analyzed in terms of seven different impact categories: climate change, ozone depletion, human toxicity, terrestrial acidification, freshwater eutrophication, marine eutrophication, and fossil depletion. Regarding straw management, two different scenarios (burial into the soil of the straw versus harvesting) were compared. The analysis showed that the environmental impact was mainly due to field emissions, the fuel consumption needed for the mechanization of field operations, and the drying of the paddy rice. The comparison between the two scenarios highlighted that the collection of the straw improves the environmental performance of rice production except that for freshwater eutrophication. To improve the environmental performance of rice production, solutions to save fossil fuel and reduce the emissions from fertilizers (leaching, volatilization) as well as methane emissions should be implemented.

Agro-ecological analysis for the EU water framework directive: an applied case study for the river contract of the Seveso basin (Italy).

The innovative approach to the protection and management of water resources at the basin scale introduced by the European Union water framework directive (WFD) requires new scientific tools. WFD implementation also requires the participation of many stakeholders (administrators, farmers and citizens) with the aim of improving the quality of river waters and basin ecosystems through cooperative planning. This approach encompasses different issues, such as agro-ecology, land use planning and water management. This paper presents the results of a methodology suggested for implementing the WFD in the case of the Seveso river contract in Italy, one of the recent WFD applications. The Seveso basin in the Lombardy region has been one of the most rapidly urbanizing areas in Italy over the last 50 years. First, land use changes in the last 50 years are assessed with the use of historical aerial photos. Then, elements of an ecological network along the river corridor are outlined, and different scenarios for enhancing existing ecological connections are assessed using indicators from graph theory. These scenarios were discussed in technical workshops with involved stakeholders of the river contract. The results show a damaged rural landscape, where urbanization processes have decimated the system of linear green features (hedges/rows). Progressive reconnections of some of the identified network nodes may significantly increase the connectivity and circuitry of the study area.

Environmental security: a geographic information system analysis approach--the case of Kenya.

Studies into the relationships between environmental factors and violence or conflicts constitute a very debated research field called environmental security. Several authors think that environmental scarcity, which is scarcity of renewable resources, can contribute to generate violence or social unrest, particularly within states scarcely endowed with technical know-how and social structures, such as developing countries. In this work, we referred to the theoretical model developed by the Environmental Change and Acute Conflict Project. Our goal was to use easily available spatial databases to map the various sources of environmental scarcity through geographic information systems, in order to locate the areas apparently most at risk of suffering negative social effects and their consequences in terms of internal security. The analysis was carried out at a subnational level and applied to the case of Kenya. A first phase of the work included a careful selection of databases relative to renewable resources. Spatial operations among these data allowed us to obtain new information on the availability of renewable resources (cropland, forests, water), on the present and foreseen demographic pressure, as well as on the social and technical ingenuity. The results made it possible to identify areas suffering from scarcity of one or more renewable resources, indicating different levels of gravity. Accounts from Kenya seem to confirm our results, reporting clashes between tribal groups over the access to scarce resources in areas that our work showed to be at high risk.