RESUMEN
Agonists of glutamate ionotropic (NMDA) and metabotropic receptors (mGluRI and mGluRIII) had the regulatory effect on ADP-ribosyl cyclase/CD38 activity in cerebellar granular cells of newborn rats. Perinatal hypoxic-ischemic brain injury was followed by dysregulation of this mechanism.
Asunto(s)
ADP-Ribosil Ciclasa/metabolismo , Agonistas de Aminoácidos Excitadores/metabolismo , Hipoxia-Isquemia Encefálica/patología , Receptores Ionotrópicos de Glutamato/agonistas , Receptores de Glutamato Metabotrópico/agonistas , Transducción de Señal , ADP-Ribosil Ciclasa 1/metabolismo , Animales , Cerebelo/efectos de los fármacos , Cerebelo/metabolismo , Femenino , Hipoxia-Isquemia Encefálica/metabolismo , Masculino , Glicoproteínas de Membrana/metabolismo , N-Metilaspartato/farmacología , Neuronas/metabolismo , RatasRESUMEN
A gene library of Anaerocellum thermophilum Z-1320 was constructed in Escherichia coli using plasmid pUC18. Clones were selected by screening for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clones expressing two beta-1,4-glucanases and two different xylanases were obtained. Both beta-1,4-glucanases were capable of degrading Avicel. Clones with xylanase activity also cleft PNPXyl, MU-Glu and MU-Cel. The genes of one beta-1,4-glucanase and one xylanase are probably located in the same region of A.thermophilum chromosome.
