RESUMEN
We studied the role of receptors with high and low affinity for fMLF chemotaxic peptide in the generation of active oxygen species by umbilical cord blood granulocytes from newborns with normal neonatal period, born after normal or complicated gestation, in children with manifestations of bacterial infection born after complicated pregnancy, and in granulocytes of non-pregnant women with normal reproductive function. Granulocytes of children born after complicated pregnancy exhibited high reactivity in induction of respiratory burst in a wide range of fMLF concentrations. The presentation of receptors with high and low affinity on granulocytes during initiation of the respiratory burst differs in children born after complicated pregnancy and in healthy babies born after normal gestation. Presumably, the detected differences result from high expression of receptors with low affinity for fMLF and disorders or immaturity of mechanisms responsible for receptor inactivation.
Asunto(s)
Sangre Fetal/metabolismo , Granulocitos/metabolismo , Recién Nacido/sangre , Receptores de Formil Péptido/fisiología , Síndrome de Respuesta Inflamatoria Sistémica/sangre , Estudios de Casos y Controles , Células Cultivadas , Enfermedades Transmisibles/sangre , Enfermedades Transmisibles/congénito , Enfermedades Transmisibles/etiología , Enfermedades Transmisibles/metabolismo , Femenino , Granulocitos/efectos de los fármacos , Granulocitos/patología , Humanos , Recién Nacido/metabolismo , N-Formilmetionina Leucil-Fenilalanina/antagonistas & inhibidores , N-Formilmetionina Leucil-Fenilalanina/farmacología , Oligopéptidos/farmacología , Embarazo , Complicaciones Infecciosas del Embarazo/sangre , Complicaciones Infecciosas del Embarazo/metabolismo , Complicaciones Infecciosas del Embarazo/patología , Receptores de Formil Péptido/metabolismo , Factores de Riesgo , Síndrome de Respuesta Inflamatoria Sistémica/etiología , Síndrome de Respuesta Inflamatoria Sistémica/metabolismo , Síndrome de Respuesta Inflamatoria Sistémica/patologíaRESUMEN
We carried out a comparative clinical and immunological examination of newborns whose mothers were at risk of infectious inflammatory diseases. Umbilical blood cell phenotype was evaluated by flow cytofluorometry. ROS level was evaluated by chemiluminescence intensity. Spontaneous production of ROS and phagocytic activity of cells in the whole umbilical blood was reduced in newborns born after complicated pregnancy. Low immunoregulatory index indicating changed CD4+/CD8+ ratio and low percentage of natural killer cells were observed in children with manifestations of bacterial infection. ROS production by isolated granulocytes and the effects of PI3K and p38 MAPK (kinases involved in the regulation of activity of NADPH oxidase responsible for the production of ROS) in the risk group infants differed from the corresponding parameters in the control group. The results indicate shifts in the phagocytosis system, immune status, and the receptor-conjugated regulatory systems of ROS generation by granulocytes in newborns at risk of infectious inflammatory diseases.