Low-frequency component of photoplethysmogram reflects the autonomic control of blood pressure.

The question of how much information the photoplethysmogram (PPG) signal contains on the autonomic regulation of blood pressure (BP) remains unsolved. This study aims to compare the low-frequency (LF) and high-frequency components of PPG and BP and assess their correlation with oscillations in interbeat (RR) intervals at similar frequencies. The PPG signal from the distal phalanx of the right index finger recorded using a reflective PPG sensor at green light, the BP signal from the left hand recorded using a Finometer, and RR intervals were analyzed. These signals were simultaneously recorded within 15 min in a supine resting condition in 17 healthy subjects (12 males and 5 females) aged 33 ± 9 years (mean ± SD). The study revealed the high coherence of LF components of PPG and BP with the LF component of RR intervals. The high-frequency components of these signals had low coherence. The analysis of the signal instantaneous phases revealed the presence of high-phase coherence between the LF components of PPG and BP. It is shown that the LF component of PPG is determined not only by local myogenic activity but also reflects the processes of autonomic control of BP.

Simulated Microgravity Induces Regionally Distinct Neurovascular and Structural Remodeling of Skeletal Muscle and Cutaneous Arteries in the Rat.

Introduction: Mechanical forces and sympathetic influences are key determinants of vascular structure and function. This study tested the hypothesis that hindlimb unloading (HU) exerts diverse effects on forelimb and hindlimb small arteries of rats in functionally different regions of the skeletal muscle and skin. Methods: Male Wistar rats were subjected to HU for 2 weeks, then skeletal muscle arteries (deep brachial and sural) and skin arteries (median and saphenous) were examined in vitro using wire myography or isobaric perfusion and glyoxylic acid staining. Results: HU increased lumen diameter of both forelimb arteries but decreased diameter of the sural artery; the saphenous artery diameter was not affected. Following HU, maximal contractile responses to noradrenaline and serotonin increased in the forelimb but decreased in the hindlimb skeletal muscle feed arteries with no change in skin arteries; all region-specific alterations persisted after endothelium removal. HU increased the sensitivity to vasoconstrictors in the saphenous artery but not in the sural artery. In the saphenous artery, initially high sympathetic innervation density was reduced by HU, sparse innervation in the sural artery was not affected. Electrical stimulation of periarterial sympathetic nerves in isobarically perfused segments of the saphenous artery demonstrated a two-fold decrease of the contractile responses in HU rats compared to that of controls. Conclusion: HU induces contrasting structural and functional adaptations in forelimb and hindlimb skeletal muscle arteries. Additionally, HU had diverse effects in two hindlimb vascular regions. Hyper-sensitivity of the saphenous artery to vasoconstrictors appears to result from the shortage of trophic sympathetic influence. Importantly, HU impaired sympathetically induced arterial vasoconstriction, consistent with the decreased sympathetic constrictor response in humans following space flight.

Phase Coupling Between Baroreflex Oscillations of Blood Pressure and Heart Rate Changes in 21-Day Dry Immersion.

INTRODUCTION: Dry immersion (DI) is a ground-based experimental model which reproduces the effects of microgravity on the cardiovascular system and, therefore, can be used to study the mechanisms of post-flight orthostatic intolerance in cosmonauts. However, the effects of long-duration DI on cardiovascular system have not been studied yet. The aim of this work was to study the effects of 21-day DI on systemic hemodynamics and its baroreflex control at rest and during head-up tilt test (HUTT). METHODS: Ten healthy young men were exposed to DI for 21 days. The day before, on the 7th, 14th, and 19th day of DI, as well as on the 1st and 5th days of recovery they were subjected to HUTT: 15 min in supine position and then 15 min of orthostasis (60°). ECG, arterial pressure, stroke volume and respiration rate were continuously recorded during the test. Phase synchronization index (PSI) of beat-to-beat mean arterial pressure (MAP) and heart rate (HR) in the frequency band of baroreflex waves (∼0.1 Hz) was used as a quantitative measure of baroreflex activity. RESULTS: During DI, strong tachycardia and the reduction of stroke volume were observed both in supine position and during HUTT, these indicators did not recover on post-immersion day 5. In contrast, systolic arterial pressure and MAP decreased during HUTT on 14th day of DI, but then restored to pre-immersion values. Before DI and on day 5 of recovery, a transition from supine position to orthostasis was accompanied by an increase in PSI at the baroreflex frequency. However, PSI did not change in HUTT performed during DI and on post-immersion day 1. The amplitude of MAP oscillations at this frequency were increased by HUTT at all time points, while an increase of respective HR oscillations was absent during DI. CONCLUSION: 21-day DI drastically changed the hemodynamic response to HUTT, while its effect on blood pressure was reduced between days 14 and 19, which speaks in favor of the adaptation to the conditions of DI. The lack of increase in phase synchronization of baroreflex MAP and HR oscillations during HUTT indicates disorders of baroreflex cardiac control during DI.

Phase synchronization of baroreflex oscillations of blood pressure and pulse interval in rats: the effects of cardiac autonomic blockade and gradual blood loss.

OBJECTIVE: Phase synchronization of arterial pressure (AP) and pulse interval (PI) oscillations in the low-frequency band (around 0.4 Hz in rats) is governed by baroreflex activity. In long-term stationary data recordings, such synchronization can be estimated by the coherence. The phase synchronization index (PSI) can be used as well. The aim of this study was to correlate PSI and the coherence of AP and PI under stationary conditions and to estimate the informativity of PSI as a measure of baroreflex activity during transient processes. APPROACH: AP and PI were recorded in conscious Wistar rats using femoral artery catheters. To study the hemodynamics during hemorrhage, blood was gradually withdrawn (20 ml × kg-1 over 30 min) through a catheter in the carotid artery. MAIN RESULTS: PSI and coherence spectra calculated from 30-minute AP and PI recordings demonstrated distinct peaks at the frequency of 0.4 Hz; these indicators correlate well with each other (Pearson r = 0.920, p  < 0.0001). Both PSI and coherence were markedly suppressed by vagal blockade (methylatropine) and tended to reduce after sympathetic blockade (atenolol). Importantly, PSI demonstrated dynamic alterations during gradual hemorrhage. During the initial approx. 10 min of hemorrhage, AP did not change but PI was noticeably shortened, and PSI increased, which indicates the activation of the baroreflex. With further blood loss, baroreflex influences were not enough to prevent blood pressure from falling, and under such conditions PSI decreased. SIGNIFICANCE: PSI, like coherence, is an informative measure of baroreflex activity under stationary conditions. In addition, PSI permits us to follow the coupling between the baroreflex oscillations of AP and PI during transient processes, which strengthens its informative value.