Inflammatory Gene Expression in Neck Perivascular and Subcutaneous Adipose Tissue in Men With Carotid Stenosis.

The inflammatory phenotype of neck adipose tissue (NAT) might reflect its involvement in the pathogenesis of carotid atherosclerosis. We investigated inflammatory gene expression in the subcutaneous and the perivascular (pericarotid) adipose tissue from patients with carotid stenosis (CS) undergoing endarterectomy and a control group of patients without significant carotid atherosclerosis undergoing thyroid surgery. Only male patients were included (n = 13 in each study group). Clinical and biochemical data along with serum leptin, adiponectin, and monocyte chemoattractant protein 1 (MCP-1) were collected. Adipose tissue samples were obtained from both the subcutaneous and pericarotid compartments. Real-time polymerase chain reaction was used to measure gene expression of macrophage markers and adipokines. The CS group had higher subcutaneous and pericarotid visfatin gene expression and higher pericarotid expression of MCP-1 and CD68 genes. The ratio between pericarotid CD206 and CD68 gene expression was similar between study groups. Adiponectin gene expression in both NAT compartments did not differ between groups, but it was negatively associated with body weight. These observations suggest that NAT, and especially the pericarotid compartment, express enhanced inflammatory properties in patients with CS, but the proportion of anti-inflammatory macrophages in advanced atherosclerosis seems to be maintained.

Neck adipose tissue - tying ties in metabolic disorders.

Upper body adipose tissue accumulation has been associated with clustering of metabolic disorders and increased cardiovascular risk. Neck circumference (NC) indicated that subcutaneous adipose tissue (SAT) in that region is an independent pathogenic depot that might account for the additional risk missed by visceral adipose tissue (VAT). Neck adipose tissue (NAT) is not only one more ectopic depot but has several particular features that might modulate its metabolic role. Besides a controversial impact on obstructive apnea syndrome, neck fat encompasses carotid arteries as an important perivascular adipose tissue (PVAT) depot. With dysfunctional changes in obesity, physiologic vascular regulation is lost and inflammatory signals accelerate atherogenesis. Unexpected was the discovery of brown and beige adipocytes in the neck of human adults. When stimulated, brown adipose tissue (BAT) dissipates energy through thermogenesis and it is associated with other favorable metabolic effects. Moreover, the neck is the region where the browning mechanism was disclosed. With this unique plastic nature, NAT revealed multiple ties, challenging dynamics and potential new therapeutic targets that might have significant implications on metabolic outcomes and vascular risk.

Scenes from the past: CT-guided endoscopic recovery of a foreign object from the cranial cavity of an ancient Egyptian mummy.

The aim of this study was to test the value of computed tomography (CT)-guided endoscopy in the scientific study of mummified remains and the recovery of unidentified objects from ancient mummified remains. CT-guided endoscopy was performed on an Egyptian mummy from the Late Period to help retrieve an unidentified object from its skull. The combined use of CT and endoscopy facilitated the recovery of the object, minimizing further damage to the remains and to the object itself. The successful recovery of the brittle object allowed detailed analysis of the item to be performed in an attempt to determine the nature of its presence in the cranial cavity. It was confirmed that the object was a monocotyledon stem fragment. Relying on the existing literature on mummification and excerebration methods in ancient Egypt, we concluded that the stick was probably used for transnasal removal of the brain. The results of this study demonstrate the great potential of CT-guided endoscopy for minimally invasive recovery of small unidentified items from mummies that could yield important information about mummification procedures and the materials used to preserve bodies.