1.
bioRxiv
; 2024 Apr 24.
Artículo
en Inglés
| MEDLINE
| ID: mdl-38712268
RESUMEN
Novel time-resolved terbium luminescence assays were developed for CDK5 and CDK2 by designing synthetic substrates which incorporate phospho-inducible terbium sensitizing motifs with kinase substrate consensus sequences. Substrates designed for CDK5 showed no phosphorylation by CDK2, opening the possibility for CDK5-specific assay development for selective drug discovery.