Oxygen Uptake, Heart Rate, and Lactate Responses for Continuous Forward Running and Stop-and-Go Running With and Without Directional Changes.

Bekraoui, N, Boussaidi, L, Cazorla, G, and Léger, L. Oxygen uptake, heart rate, and lactate responses for continuous forward running and stop-and-go running with and without directional changes. J Strength Cond Res 34(3): 699-707, 2020-The purpose of this study is to measure and quantify the differences for the gross oxygen uptake (V[Combining Dot Above]O2), heart rate (HR), and blood lactate ([LA]) responses to various modes of in-line running. Twelve healthy participants did an indirect continuous multistage field test to determine the maximal aerobic speed (MAS) before randomly performing in separate sessions 1 of the 3 running protocols at 3 intensities (60, 70, and 80% MAS) (a) in-line continuous running (IR), (b) fractioned running (FR) with stop and go every 20 m, and (c) 20-m shuttle run (SR). V[Combining Dot Above]O2 varied between 34.2 ± 2.5 and 54.6 ± 5.4 ml·kg·min, HR between 141 ± 14 and 184 ± 9 b·min, and [LA] between 4.2 ± 0.8 and 6.6 ± 0.9 mmol·L. In-line continuous running V[Combining Dot Above]O2 was lower than those of SR and FR at all intensities (p < 0.05). However, SR V[Combining Dot Above]O2 were higher than those of FR at 80% MAS only. Heart rate was similar in SR and FR but lower in IR at all intensities (p < 0.05). No significant differences were recorded in [LA]. Because only V[Combining Dot Above]O2 was affected by the introduction of a 180° angle directional change (SR vs. FR) at 80% MAS, the use of HR to assess such activities at such intensities will result in underestimating workloads. This study offers new insights into V[Combining Dot Above]O2, HR, and [LA] associated with accelerations while running with and without directional changes at submaximal speeds.

Correlation of resistin with inflammatory and cardiometabolic markers in obese adolescents with and without metabolic syndrome.

OBJECTIVE: The link between plasma resistin and obesity-related cardiometabolic disorders in children remains debatable. This study assessed the relationships of plasma resistin with cardiovascular risk factors, pro-inflammatory markers and insulin resistance index (HOMA-IR) in obese (Ob) adolescents and obese adolescents with metabolic syndrome (Ob-MS) compared to healthy controls (CO). METHODS: 114 obese adolescents (60 Ob, age 13.6 ± 0.9 years, BMI 28.0 ± 2.2 kg/m(2), and 54 Ob-MS, age 13.8 ± 1.0 years, BMI 32.5 ± 4.8 kg/m(2)) and 37 CO (age 13.7 ± 0.8 years, BMI 22.8 ± 0.8 kg/m(2)) were studied. Anthropometrics, cardiac variables as well as fasting plasma concentrations of lipids, glucose, insulin, and adipocytokines (resistin, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP)) were measured. HOMA-IR was calculated, and the presence of MS was assessed. RESULTS: Plasma resistin was significantly higher in Ob-MS than in both Ob and CO and was correlated with anthropometric, cardiovascular, pro-inflammatory markers and several components of MS as was HOMA-IR in Ob and Ob-MS. With increasing the number of MS components, plasma resistin, pro-inflammatory markers, and HOMA-IR were also increased. Multiple regression models highlighted significant correlation between resistin and both HOMA-IR (r = 0.40, p < 0.05) and systolic blood pressure (r = 0.63, p < 0.01) in Ob-MS. CONCLUSION: These results support the hypothesis that there is an association between circulating resistin and childhood obesity-related inflammatory and cardiometabolic events.

Walk-run transition speed training as an efficient exercise adjunct to dietary restriction in the management of obesity: a prospective intervention pilot study.

OBJECTIVE: The aim of this study was to test the utility of preferred walk-run transition speed (WRTS) in exercise training adjunct to dietary restriction for obesity management in healthy obese women. MATERIALS AND METHODS: 37 obese women (age: 35 ± 9 years, body mass index (BMI): 34.9 ± 4.6 kg/m(2)) were assigned to an intervention pilot study during 6 months of restricted diet alone (RD) followed by 6 months of RD combined with WRTS (RD and WRTS) as a training exercise. Body mass, waist circumference (WC), fat mass (FM), fat free mass (FFM), active cell mass (ACM), fasting glucose, serum lipids (triacylglycerol (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), apo-lipoproteins A1 (ApoA1) and B (ApoB)], leptin and insulin concentrations, and HOMA-IR were assessed at baseline (T0), at the end of the RD alone (T1), and at the end of the RD and WRTS programme (T2). RESULTS: Mean weight loss was 8.6 ± 4.9 kg and 2.2 ± 2.9 kg for (T0-T1) and (T1-T2), respectively. Significant BMI and WC reductions were reported at T1 and T2. FM decreased significantly both with RD and with RD and WRTS training whereas FFM and ACM increased with RD and WRTS training only. TG decreased significantly with the two phases of the programme. A significant increase in HDL-C, and a decrease in LDL-C and TC/HDL-C ratio were noticed with RD and WRTS training. Heart rate monitored in training improved significantly after RD and WRTS training. A significant relationship (r = 0.542, p < 0.02) was demonstrated between reductions in serum leptin and insulin concentrations observed with both RD and WRTS training. CONCLUSION: The addition of WRTS training to RD promoted a greater reduction in body mass, WC, FM, leptin and insulin concentrations, improved metabolic and cardiovascular risk factors, and enhanced cardiovascular fitness.

Validation for quantification of immunoglobulins by Fourier transform infrared spectrometry.

BACKGROUND: The objective of this study was to develop a robust quantification method for simultaneously analyzing molecules in human plasma using the Fourier transform infrared (FT-IR) system with a partial least square (PLS) regression. METHODS: Plasma spectra were analyzed from 4000 to 500 cm(-1) (with 2.0 cm(-1) of resolution and 32 scans), and the molecule concentrations (IgA, IgG, IgM) were measured blindly by using a cross-validation model prepared by PLS analysis of data from 135 samples. RESULTS: There was a significant correlation between the FT-IR predicted concentration and the concentration obtained with the clinical reference method: R(2)=0.98 (IgA), R(2)=0.98 (IgG), and R(2)=0.97 (IgM). The root mean square error of prediction (RMSEP) was 0.05 g.L(-1) (IgA), 0.4 g.L(-1) (IgG), and 0.03 g.L(-1) (IgM). Variability of inter-experimenter reproducibility was less than 2%. The interchangeability of the two methods was studied by using the Bland-Altman method. CONCLUSIONS: Together with PLS analysis, FT-IR spectrometry appears to be an easy-to-use and accurate method to determine multianalyte concentrations in dried human plasma. It could be an alternative tool for rapidly quantifying many molecules after developing a specific predictive model.