RESUMEN
Approximately 21% of children suffer from some form of physical abuse. It is hypothesized that when an individual hits a child some of that person's DNA will be deposited onto the child's skin. As yet, no one has reported a method of sampling DNA from the skin of this vulnerable group of individuals. We have sampled DNA from several facial areas of 30 children aged 5 years of age and under. The results show that it is possible to swab the faces of this age group without distressing them or contaminating the samples. Additionally the results indicate that the DNA obtained is almost entirely that of the subject, with little nondonor DNA being observed.
Asunto(s)
Maltrato a los Niños/diagnóstico , Dermatoglifia del ADN/métodos , Cara , Enfermería Forense/métodos , Manejo de Especímenes/métodos , Cefalometría , Niño , Maltrato a los Niños/psicología , Maltrato a los Niños/estadística & datos numéricos , Preescolar , Estudios de Factibilidad , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Evaluación en Enfermería , Investigación en Evaluación de Enfermería , Aceptación de la Atención de Salud , Reacción en Cadena de la Polimerasa/métodos , Psicología Infantil , Factores de Riesgo , Manejo de Especímenes/instrumentación , Manejo de Especímenes/enfermería , Manejo de Especímenes/psicologíaRESUMEN
DNA profiling of biological trace evidence has been used for many years. The application of this technique specifically to the DNA profiling of earprints has not to date been thoroughly investigated. This report presents the results of 60 earprints collected from three healthy adult volunteers under controlled laboratory conditions. DNA profile analysis revealed that high levels of non-donor alleles are observed when earprints are collected for DNA profiling. The source of these non-donor alleles is investigated and the impact that their presence within the profile may have on the use of this technique is discussed.
Asunto(s)
Dermatoglifia del ADN , Oído Externo , Ciencias Forenses/métodos , Adulto , Alelos , Humanos , Técnicas de Amplificación de Ácido Nucleico , Reproducibilidad de los Resultados , Manejo de EspecímenesRESUMEN
In 1923, within the Manual of Police technique, Edmond Locard published what is commonly known as the Doctrine of Exchange; a series of rules related to the exchange of trace evidence between the victim and offender. Although at the time of publication these rules principally applied to trace evidence related to print (for exchange finger print or shoeprint), fibre and blood, today one can add the very substance that defines each human being -- DNA. Since th first use of DNA evidence to help identify an offender in the Pitchfork Murders of 1986, the use of DNA within forensic science has developed from its humble days within a single experimental laboratory at the University of Leicester to a multi-million pound industry. It thus seams fitting that this forensic web watch should originate from the very University where the use of DNA in forensic science was conceived, drawing the readers attention to a number of sites which can be used as an introduction to the concept of the use of DNA in forensic science today.
