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1.
Artículo en Ruso | MEDLINE | ID: mdl-22830277

RESUMEN

AIM: Comparative study of the microbiota of neonates in relation to vaginal and intestinal microflora state of conditionally healthy primipara pregnant women. MATERIALS AND METHODS: Bacteriological study of vaginal and intestinal microflora of 24 conditionally healthy primipara pregnant women at week 32 and 38 of pregnancy as well as bacteriological study of meconium and feces at day 5 of their neonates was performed. RESULTS: Despite detection of lactobacilli by delivery in all the pregnant women their quantity was significantly lower than normal. In colon microflora dysbiotic changes corresponding to grade 1 (46.7%) and 2 (53.3%) dysbacteriosis were detected. In 60.0% of cases of conditionally healthy neonates various microorganism species were isolated from meconium. At day 5 in feces of neonates at natural feeding lactobacilli (85.8%), bifidobacteria and escherichia (71.4% each), enterococci (100%) were detected. CONCLUSION: The course of dysbiotic changes in vagina and intestines of conditionally healthy pregnant women had subclinical level. Detection of microorganisms in meconium in conditionally healthy neonates could be explained by translocation mechanism.


Asunto(s)
Heces/microbiología , Recién Nacido/fisiología , Intestinos/microbiología , Meconio/microbiología , Consorcios Microbianos , Vagina/microbiología , Adolescente , Adulto , Bifidobacterium/aislamiento & purificación , Enterococcus/aislamiento & purificación , Escherichia/aislamiento & purificación , Femenino , Humanos , Lactobacillus/aislamiento & purificación , Paridad , Embarazo , Tercer Trimestre del Embarazo , Factores de Tiempo
2.
Vestn Otorinolaringol ; (4): 17-9, 2010.
Artículo en Ruso | MEDLINE | ID: mdl-21105338

RESUMEN

The objective of the present work was to study microflora in the middle ear of 100 patients aged from 30 to 70 years presenting with chronic purulent otitis media (CPOM) by microbiological and genetic (PCR) methods. An important role of persistence factors, pathogenicity, and microbial antibiotic resistance in the development of CPOM was demonstrated. The frequency of occurrence of herpes and papilloma viruses was estimated. The most common form of the mixed infection was two-component virobacterial associations (46.2%). Three-component associations of viruses with bacteria (Chlamydiae or Mycoplasmas) formed less frequently (34.6%). In 19.2% of the cases, yeast-like fungi of the genus Candida were identified in these associations.


Asunto(s)
Oído Medio/microbiología , Otitis Media Supurativa/microbiología , Adulto , Anciano , Antibacterianos/farmacología , Bacterias/clasificación , Bacterias/efectos de los fármacos , Bacterias/aislamiento & purificación , Candida/clasificación , Candida/aislamiento & purificación , Enfermedad Crónica , Oído Medio/virología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Otitis Media Supurativa/virología , Virus/clasificación , Virus/aislamiento & purificación
3.
Artículo en Ruso | MEDLINE | ID: mdl-11871292

RESUMEN

The microflora of palatal tonsils was studied in 84 children with chronic tonsillitis in comparison with that in the control group of 38 healthy children. In most of the sick children viral-bacterial and less frequently viral-bacterial-fungal associations were detected with the prevalence of reo- and adenoviruses, Epstein-Barr viruses, coagulase negative staphylococci and Staphylococcus aureus, as well as peptostreptococci. Adhesive activity and persistence factors among the main bacterial pathogens were shown to be widely prevalent. The depth of the lesion of tonsillar tissue by the infective agents of bacterial and fungal nature, as well as their persistence potential, depended on the taxonomic position of these microorganisms.


Asunto(s)
Bacterias/aislamiento & purificación , Candida/aislamiento & purificación , Tonsilitis/microbiología , Tonsilitis/virología , Virus/aislamiento & purificación , Adolescente , Bacterias/clasificación , Adhesión Bacteriana , Candida/clasificación , Niño , Preescolar , Enfermedad Crónica , Humanos , Virus/clasificación
4.
Artículo en Ruso | MEDLINE | ID: mdl-10852058

RESUMEN

The complex examination of 72 patients with acute ileus (AI) of nontumor nature with different severity of endotoxicosis was carried out. The study revealed that AI was accompanied by deep suppression of the immunosecretory and motor evacuatory function of the small intestine, as well as by its pronounced bacterial contamination, mainly due to the significant quantitative prevalence of Gram-negative microflora. The combination of these factors played the key role in the increase of the permeability of the enteric barrier for symbiotic microflora and its massive translocation from the intestinal tract to the internal organs of the body (peritoneal exudate, portal bed), which directly correlated with the severity of endotoxicosis in AI patients. The deficiency of the barrier function of the liver was accompanied by the penetration of infective agents into the general blood stream, thus causing the development of endotoxic shock in AI patients. The analysis of the results thus obtained made it possible to determine the main ways for the elimination of intestinogenic intoxication in AI; they should be aimed at the bacterial decontamination of the small intestine, the restoration of its motor evacuatory and protective barrier functions, the liquidation of portal and systemic bacteremia, the correction of the functional deficiency of the liver.


Asunto(s)
Bacterias Aerobias/fisiología , Bacterias Anaerobias/fisiología , Traslocación Bacteriana/inmunología , Bacilos Gramnegativos Anaerobios Facultativos/fisiología , Obstrucción Intestinal/inmunología , Obstrucción Intestinal/microbiología , Intestino Delgado/inmunología , Intestino Delgado/microbiología , Enfermedad Aguda , Líquido Ascítico/inmunología , Líquido Ascítico/microbiología , Endotoxemia/inmunología , Endotoxemia/microbiología , Humanos , Inmunoglobulinas/análisis
5.
Vestn Khir Im I I Grek ; 157(4): 46-9, 1998.
Artículo en Ruso | MEDLINE | ID: mdl-9825437

RESUMEN

A complex investigation of 72 patients, with acute bowel obstruction (ABO) having clinico-laboratory signs and symptoms of endotoxicosis (ET), was carried out. It was proven that ABO was accompanied by profound decrease of the immuno-secretory function of the small bowel and its extensive bacterial contamination as a result of significant increase in the concentration of gram-negative symbiotic microflora, which leads to increased permeability of bowel barrier. Consequently there is massive translocation of the internal medium of the organism by enteral microflora and most of all in the portal zone and in the presence of inadequate hepatic barrier function--the systemic blood supply which corresponds to the clinical picture of endotoxic shock.


Asunto(s)
Bacteriemia/etiología , Obstrucción Intestinal/complicaciones , Intestinos/microbiología , Sistema Porta/microbiología , Enfermedad Aguda , Bacteriemia/inmunología , Bacteriemia/microbiología , Bacterias/aislamiento & purificación , Humanos , Inmunoglobulinas/análisis , Obstrucción Intestinal/etiología , Obstrucción Intestinal/inmunología , Intestinos/inmunología , Periodo Intraoperatorio , Sistema Porta/inmunología , Periodo Posoperatorio , Índice de Severidad de la Enfermedad
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