Variation and genetic control of individual recombination rates in Norwegian Red dairy cattle.

Meiotic recombination is an important evolutionary mechanism that breaks up linkages between loci and creates novel haplotypes for selection to act upon. Understanding the genetic control of variation in recombination rates is therefore of great interest in both natural and domestic breeding populations. In this study, we used pedigree information and medium-density (∼50K) genotyped data in a large cattle (Bos taurus) breeding population in Norway (Norwegian Red cattle) to investigate recombination rate variation between sexes and individual animals. Sex-specific linkage mapping showed higher rates in males than in females (total genetic length of autosomes = 2,492.9 cM in males and 2,308.9 cM in females). However, distribution of recombination along the genome showed little variation between males and females compared with that in other species. The heritability of autosomal crossover count was low but significant in both sexes (h2 = 0.04 and 0.09 in males and females, respectively). We identified 2 loci associated with variation in individual crossover counts in female, one close to the candidate gene CEP55 and one close to both MLH3 and NEK9. All 3 genes have been associated with recombination rates in other cattle breeds. Our study contributes to the understanding of how recombination rates are controlled and how they may vary between closely related breeds as well as between species.

Cellular multiparametric MRI of neural stem cell therapy in a rat glioma model.

Cellular multiparametric magnetic resonance imaging (MRI) provided an in vivo visualisation of neural stem cells' (NSCs) tropism for gliomas in the rat brain. NSCs were magnetically labelled in vitro with the bimodal gadolinium-based contrast agent, gadolinium rhodamine dextran (GRID), and injected into the contralateral hemisphere to the developing tumour. Contrast-to-noise measurements showed that GRID-labelled cells induced a signal attenuation on both T2-, T2(*)-weighted images, and a modest signal gain on T1-weighted images. Tumour development and progression were longitudinally monitored in vivo by serial MR scanning. Measurements of tumour volume and tumour progression over time in terms of tumour doubling time showed a tendency towards a reduced tumour growth in NSC-treated animals. MR findings of migration and infiltration of tumours by labelled NSCs were corroborated with immunohistopathology, where labelled cells were detected in the corpus callosum at the tumour border and dispersed in the solid tumour tissue. Immunohistopathology also revealed that macrophages invaded the tumour tissue and in some cases engulfed GRID-labelled stem cells. No significant difference in macrophage recruitment between NSC-treated and vehicle-treated animals were detected, indicating that magnetically labelled NSC do not increase macrophage invasion of tumour tissue. Our findings demonstrate that cellular multiparametric MRI provides a valuable tool for in vivo dynamic monitoring of tumour-directed neural stem cell migration as well as therapeutic efficacy.

NG2 expression regulates vascular morphology and function in human brain tumours.

Tumour angiogenesis is a tightly regulated process involving cross-talk between tumour cells and the host tissue. The underlying mechanisms that regulate such interactions remain largely unknown. NG2 is a transmembrane proteoglycan whose presence on transformed cells has been demonstrated to increase proliferation in vitro and angiogenesis in vivo. To study the effects of NG2 during tumour growth and progression, we engineered an NG2 positive human glioma cell line (U251-NG2) from parental NG2 negative cells (U251-WT) and implanted both cell types stereotactically into immunodeficient nude rat brains. The tumours were longitudinally monitored in vivo using multispectral MRI employing two differently sized contrast agents (Gd-DTPA-BMA and Gadomer) to assess vascular leakiness, vasogenic oedema, tumour volumes and necrosis. Comparisons of Gd-DTPA-BMA and Gadomer revealed differences in their spatial distribution in the U251-NG2 and U251-WT tumours. The U251-NG2 tumours exhibited a higher leakiness of the larger molecular weight Gadomer and displayed a stronger vasogenic oedema (69.9 +/- 15.2, P = 0.018, compared to the controls (10.7 +/- 7.7). Moreover, immunohistochemistry and electron microscopy revealed that the U251-NG2 tumours had a higher microvascular density (11.81 +/- 0.54; P = 0.0010) compared to controls (5.76 +/- 0.87), with vessels that displayed larger gaps between the endothelial cells. Thus, tumour cells can regulate both the function and structure of the host-derived tumour vasculature through NG2 expression, suggesting a role for NG2 in the cross-talk between tumour-host compartments.

Recombinant porcine somatotropin alters performance and carcass characteristics of heavyweight swine and swine fed alternative feedstuffs.

The efficacy of recombinantly derived porcine somatotropin (rpST) in improving the performance and carcass characteristics of heavyweight finishing pigs was determined. In Study 1, 36 pigs were killed for determination of initial carcass composition at 102 kg, and 36 pigs each were given 0, 1.5, 3, 6, or 9 mg of rpST/d from 102 to 136 kg live weight. Corn-soybean meal diets fed contained 19.8% CP and greater than 1% lysine. Study 2 tested the effects of 3 mg/d on pigs for 4 wk from 84 kg BW fed a corn-soybean meal or a triticale-barley-peas diet. Performance variables (ADG, average daily feed intake [ADFI], and feed efficiency [FE]) were measured weekly. Treatment with rpST increased (P less than .01) ADG and FE and decreased (P less than .01) ADFI in both studies. In Study 1, leaf fat, backfat thickness, belly thickness, and carcass fat were all decreased (P less than .01) linearly by rpST. Loin eye area (LEA), total trimmed lean, and total protein content were increased quadratically (P less than .01). At the 3-mg dose, ADFI, ADG, and FE were 87, 130, and 137% of control, and LEA, backfat, total protein gain, and total fat gain were 107, 80, 136, and 52% of control. Loin eye area was not increased (P greater than .05) in the pigs in Study 2; however, backfat thickness was reduced 16% by rpST (P less than .01). The effects of rpST were the same on both dietary regimens (P greater than .05). These studies demonstrate the effectiveness of rpST in increasing ADG and carcass leanness and improving FE in heavyweight pigs and in pigs fed alternative feedstuffs.

Antimicrobial Activity of Butylated Hydroxyanisole and Potassium Sorbate Against Natural Microflora in Raw Turkey Meat and Salmonella typhimurium in Cooked Turkey Meat 1.

The antimicrobial activity of butylated hydroxyanisole (BHA) (100 ppm) and potassium sorbate (1000 ppm), individually and in combination, was evaluated against growth of the natural microbial flora in raw turkey meat and against Salmonella typhimurium inoculated into cooked turkey meat. Growth of the natural flora was not inhibited by using either BHA or sorbate alone; however, slight inhibition was shown using a combination of the two. BHA, sorbate and a combination were effective to the same extent in preventing growth of naturally present gram-negative organisms. Sorbate and the BHA-sorbate combination did not differ in their effectiveness and were more effective than BHA alone in reducing numbers of S. typhimurium in cooked turkey.

Separation and characterization of the troponin components from bovine cardiac muscle.

The three major components of bovine cardiac troponin were separated by successive chromatography on sulfopropyl-Sephadex and DEAE-Sephadex columns in the presence of 6 M urea. All three of the bovine cardiac troponin subunits were necessary to restore full troponin activity in both skeletal and cardiac actomyosin ATPase assay systems. The 38,000-dalton subunit bound tropomyosin, and the 20,000-dalton subunit bound calcium, like skeletal TN-T and TN-C, respectively. The 28,000 component, although presumably analogous to skeletal TN-I, gave very little inhibition of actomyosin ATPase activity. Differences between cardiac and skeletal troponin subunits were also found in the elution patterns from ion exchange columns and in amino acid composition, thus demonstrating a significant muscle-type specificity.