Nineteenth century French rose (Rosa sp.) germplasm shows a shift over time from a European to an Asian genetic background.

Hybridization with introduced genetic resources is commonly practiced in ornamental plant breeding to introgress desired traits. The 19th century was a golden age for rose breeding in France. The objective here was to study the evolution of rose genetic diversity over this period, which included the introduction of Asian genotypes into Europe. A large sample of 1228 garden roses encompassing the conserved diversity cultivated during the 18th and 19th centuries was genotyped with 32 microsatellite primer pairs. Its genetic diversity and structure were clarified. Wide diversity structured in 16 genetic groups was observed. Genetic differentiation was detected between ancient European and Asian accessions, and a temporal shift from a European to an Asian genetic background was observed in cultivated European hybrids during the 19th century. Frequent crosses with Asian roses throughout the 19th century and/or selection for Asiatic traits may have induced this shift. In addition, the consistency of the results with respect to a horticultural classification is discussed. Some horticultural groups, defined according to phenotype and/or knowledge of their pedigree, seem to be genetically more consistent than others, highlighting the difficulty of classifying cultivated plants. Therefore, the horticultural classification is probably more appropriate for commercial purposes rather than genetic relatedness, especially to define preservation and breeding strategies.

Archaeal and bacterial community composition of sediment and plankton from a suboxic freshwater pond.

We studied the composition of archaeal and bacterial communities present in the sediment and plankton of a shallow suboxic-to-anoxic freshwater pond with high organic matter input, as an example of a kind of inland freshwater system widely distributed in forests of temperate regions. Molecular surveys based on small subunit rRNA genes showed a remarkably high diversity of lineages within the Bacteria, with a total of 18 phyla or candidate divisions being detected, in addition to a few highly divergent phylotypes of unknown affiliation. We identified members of the five subdivisions of the Proteobacteria, as well as Acidobacteria, Verrucomicrobia, Planctomycetes, Bacteroidetes, Chlorobi, Actinobacteria, Firmicutes, Chloroflexi, Gemmatimonadetes, Spirochaetes, Fibrobacteres and the candidate divisions OD1, OP11, TM6, WS1, WS6 and Termite Group 1 ("Endobacteria"). Candidate division OD1 and beta-Proteobacteria were dominant in the environmental libraries of plankton and sediment, respectively. Archaea were also very diverse, but only members of the Euryarchaeota, including Methanosarcinales, Methanomicrobiales and some divergent lineages, were identified. The application of various species richness estimators confirmed the highly diverse nature of both plankton and sediment samples. The pond is a microbial-based complex ecosystem mainly fueled by the degradation of allochthonous organic matter that maintains tightly coupled carbon and sulfur cycles.