Sterilized human skin graft with a dose of 25 kGy provides a privileged immune and collagen microenvironment in the adhesion of Nude mice wounds.

This study aimed to report the effects of different doses of ionizing radiation on inflammatory and repair stage of human skin graft adherence in Nude mice wounds. Animals were divided into transplanted with irradiated human skin grafts (IHSG) at 25 and 50 kGy (IHSG 25 kGy; IHSG 50 kGy) and non-IHSG and euthanized on the 3rd, 7th and 21st days after the surgery, by gross and microscopic changes, immunostaining for human type I collagen (Col I) and mouse Col I and Col III and inflammatory cells. We found an effectiveness of human split-thickness graft adherence in mice transplanted with IHSG 25 kGy, as well decrease in dermo-epidermal necrosis and neutrophils, lower loss of skin thickness, epithelization and neo-vascularization. Day 21 post-transplantation with IHSG 25 kGy was observed a well-preserved human skin in the border of the graft, a prominent granulation tissue in an organization by proliferated fibroblasts, Col III deposition and increased B-cells and macrophages. A complete adherence of human skin graft occurred with IHSG 25 kGy. We suggest that the ionizing radiation at 25 kGy mediates inflammation and the repair stage of human skin graft adherence in murine model, thus emerging as a potential tool in healing cutaneous wounds.

Condição de saúde bucal de idosos institucionalizados em Araguaína/TO / Oral health status of institucionalized elderly in Araguaína/TO

O objetivo deste estudo epidemiológico foi caracterizar a condição de saúde bucal de idosos institucionalizados na cidade de Araguaína/TO, durante o ano de 2013. Foram realizados entrevistas e exames intrabucais em 38 indivíduos para determinação do perfil de idosos quanto à idade; tempo de institucionalização; uso de fumo; tempo decorrido da última visita ao dentista; utilização de fio dental; uso de medicamentos; uso de prótese dentária; frequência de escovação; fluxo salivar; índice de placa (IP); índice de sangramento gengival (ISG); profundidade de sondagem (PS); índice de recessão gengival (IRG) e índice CPO-D. Os pacientes entrevistados possuíam idades entre 65 e 101 anos; 65,79% estavam institucionalizados há três anos ou mais; 28,95% utilizavam o fumo; o tempo médio da última visita ao dentista foi de 12,48 anos; nenhum utilizava fio dental; 97,37% faziam uso de medicamentos; 63,16% eram edêntulos e apenas 26,31% utilizavam prótese dentária e 36,84% dos idosos não realizavam escovação. Da amostra, 50% apresentava hipossalivação. Dentre os idosos que possuíam dentes (n = 12), o IP variou de 69% a 100% e o ISG, de 0% até 65,60%; a condição mais severa de PS (de 4 até 5mm) foi observada em 41,67% da amostra e de IRG (de 6 até 8mm), em 41,67%, o índice CPO-D apresentou uma média de 30,53. Os resultados observados sugerem que para o grupo estudado fatores como a falta ou inadequada higienização, uso de medicamentos e fumo podem comprometer a saúde bucal desencadeando impactos negativos para a saúde...

The aim of this epidemiological study was to characterize the oral health status of institutionalized elderly in Araguaína/ TO, during 2013. Interviews and intraoral examinations were performed in 38 subjects to determine the profile of the elderly regarding the age, duration of institutionalization, use of tobacco, time since last visit to the dentist, utilization of dental floss, use of medications, denture use, frequency of brushing, salivary flow (SF), plaque index (PI), gum bleeding index (GBI), probing depth (PD), gum recession index (GRI) and DMFT index. The patients interviewed were between 65 and 101 years old, 65.79% were institutionalized for three years or more, 28.95% used tobacco, the average time since the last visit to the dentist was 12.48 years, none used dental floss, 97.37% used medications, 63.16% were edentulous and only 26.31% used dentures, and 36.84% of seniors did not perform brushing, 50% of the sample had hyposalivation. Among the subjects who possessed teeth (n = 12), PI ranged from 69% to 100% and GBI, from 0% to 65.60%, the most severe condition of PD (4 to 5mm) was observed in 41.67% and GRI (6 to 8mm) in 41.67% of the sample and the DMFT index showed an average of 30.53. The results observed suggest that for the group studied, factors such as the lack or inadequate hygienization, use of medications and tobacco can compromise the oral health triggering negative impacts on health...

[Evolution of the neonatal screening program in the state of Tocantins]. / Evolução do programa de triagem neonatal no estado do Tocantins.

OBJECTIVE: To evaluate the Neonatal Screening Program in the State of Tocantins from 1995 to 2011. MATERIALS AND METHODS: Data collection was conducted by means of interviews with those responsible for the service, by the analysis of medical records of patients diagnosed with phenylketonuria (PKU) and congenital hypothyroidism (CH) that were enrolled in the program, and by interviews with parents and/or guardians of the patients monitored. RESULTS: Program coverage increased from 32.3% to 76.6% after the implementation of the National Newborn Screening Program (PNTN). The prevalence of PKU and CH was 1:28,309 and 1:4,632 live births, respectively. The mean ages at the collection of the first blood sample (PKU: 9.6 ± 6.3 days; CH: 13.3 ± 10.3 days) and at the beginning of the treatment (PKU: 57.0 ± 17.6 days; CH: 95,6 ± 57.6 days) were greater than recommended by the Ministry of Health. The quality of monitoring was considered satisfactory by 100% of the parents. CONCLUSION: Although there have been great developments in neonatal screening program in this state, there is need for greater government incentives to optimize the program and to make the PNTN advance to its next phases.

Evolução do programa de triagem neonatal no estado do Tocantins / Evolution of the neonatal screening program in the state of Tocantins

OBJETIVO: Avaliar o Programa de Triagem Neonatal do Estado do Tocantins de 1995 a 2011. MATERIAIS E MÉTODOS: A coleta de dados foi realizada por meio de entrevista com os responsáveis pelo serviço, por análise de prontuários de pacientes com diagnóstico de fenilcetonúria (PKU) ou hipotireoidismo congênito (HC) atendidos pelo programa e por entrevista com pais e/ou responsáveis por pacientes em acompanhamento. RESULTADOS: A cobertura de triagem neonatal aumentou de 32,3% para 76,6% depois da implantação do Programa Nacional de Triagem Neo­natal (PNTN). A prevalência de PKU e de HC no período analisado foi de 1:28.309 e de 1:4.632 nascidos vivos, respectivamente. A idade média das crianças na coleta da primeira amostra de sangue (PKU: 9,6 ± 6,3 dias; HC: 13,3 ± 10,3 dias) e no início do tratamento (PKU: 57,0 ± 17,6 dias; HC: 95,6 ± 57,6 dias) foi superior às preconizadas pelo Ministério da Saúde. A avaliação dos pais sobre a qualidade do acompanhamento realizado foi classificada como satisfatória por 100% dos entrevistados. CONCLUSÃO: Embora tenha havido grande evolução do programa de triagem neonatal deste Estado, há necessidade de maior incentivo governamental para que o programa seja otimizado e possa avançar para as fases seguintes do PNTN.

OBJECTIVE: To evaluate the Neonatal Screening Program in the State of Tocantins from 1995 to 2011. MATERIALS AND METHODS: Data collection was conducted by means of interviews with those responsible for the service, by the analysis of medical records of patients diagnosed with phenylketonuria (PKU) and congenital hypothyroidism (CH) that were enrolled in the program, and by interviews with parents and/or guardians of the patients monitored. RESULTS: Program coverage increased from 32.3% to 76.6% after the implementation of the National Newborn Screening Program (PNTN). The prevalence of PKU and CH was 1:28,309 and 1:4,632 live births, respectively. The mean ages at the collection of the first blood sample (PKU: 9.6 ± 6.3 days; CH: 13.3 ± 10.3 days) and at the beginning of the treatment (PKU: 57.0 ± 17.6 days; CH: 95,6 ± 57.6 days) were greater than recommended by the Ministry of Health. The quality of monitoring was considered satisfactory by 100% of the parents. CONCLUSION: Although there have been great developments in neonatal screening program in this state, there is need for greater government incentives to optimize the program and to make the PNTN advance to its next phases.