Validation of a Football Helmet Finite Element Model and Quantification of Impact Energy Distribution.

Head injury in contact sports can be mitigated, in part, through the enhancement of protective helmets that may be enabled by detailed finite element models. However, many contemporary helmet FE models include simplified geometry and material properties and have limited verification and validation over a representative range of impact conditions. To address these limitations, a detailed numerical model of a modern football helmet was developed, integrated with two headforms and assessed for 60 impact conditions with excellent ratings (0.79-0.93). The strain energy of the helmet components was investigated for eight impact locations and three impact speeds. In general, the helmet shell had the highest strain energy followed by the compression shocks; however, the facemask and straps had the highest strain energy for impacts involving the facemask. The component strain energy was positively correlated with the Head Injury Criterion, while the strain energy was not strongly correlated with the Brain Injury Criterion due to the dependence on rotational kinematics. This study demonstrated the applicability of a detailed football helmet finite element model to investigate a range of impact conditions and to assess energy distribution as a function of impact location and severity as a means of future helmet optimization.

355-nm high spectral resolution airborne lidar LNG: system description and first results.

A high spectral resolution (HSR) measurement capability in the ultraviolet has been added to the 3-wavelength-2-polarization-backscatter lidar LNG (lidar aerosols nouvelle génération) and tested during several flights. The system includes a Mach-Zehnder interferometer (MZI) as a spectral discriminator and does not require any frequency locking between the emitter and the interferometer. Results obtained during test flights show that the backscatter and extinction coefficients at 355 nm can be measured with a relative precision of 10% for 60 m and 240 m vertical resolution, respectively, in aerosol layers of 10-6 m-1 sr-1 backscatter coefficient with a 30-km horizontal resolution. The same relative precision is obtained in cirrus clouds of a 2×10-5 m-1 sr-1 backscatter coefficient for the same vertical resolution and a horizontal resolution reduced to 5 km. The capacity of the system to perform wind velocity measurements is also demonstrated with precisions in the range of 1 to 2 ms-1. Particle-to-total backscatter ratio and line-of-sight speed measurements have been performed on ground echoes; averaged data show biases less than 1% and 0.15 ms-1, respectively.

The participation of AtXPB1, the XPB/RAD25 homologue gene from Arabidopsis thaliana, in DNA repair and plant development.

Nucleotide excision repair in Arabidopsis thaliana differs from other eukaryotes as it contains two paralogous copies of the corresponding XPB/RAD25 gene. In this work, the functional characterization of one copy, AtXPB1, is presented. The plant gene was able to partially complement the UV sensitivity of a yeast rad25 mutant strain, thus confirming its involvement in nucleotide excision repair. The biological role of AtXPB1 protein in A. thaliana was further ascertained by obtaining a homozygous mutant plant containing the AtXPB1 genomic sequence interrupted by a T-DNA insertion. The 3' end of the mutant gene is disrupted, generating the expression of a truncated mRNA molecule. Despite the normal morphology, the mutant plants presented developmental delay, lower seed viability and a loss of germination synchrony. These plants also manifested increased sensitivity to continuous exposure to the alkylating agent MMS, thus suggesting inefficient DNA damage removal. These results indicate that, although the duplication seems to be recent, the features described for the mutant plant imply some functional or timing expression divergence between the paralogous AtXPB genes. The AtXPB1 protein function in nucleotide excision repair is probably required for the removal of lesions during seed storage, germination and early plant development.

Mach-zehnder interferometer as a spectral analyzer for molecular Doppler wind lidar.

The theoretical performance of a Mach-Zehnder interferometer used as a spectral analyzer for wind-speed measurement by direct-detection Doppler lidar is presented. The interferometer is optimized for measurement of wind velocity from the signal backscattered by the molecules. Two arrangements are proposed, involving two detection channels (DMZ) or four detection channels (QMZ). Using the assumption of a pure molecular signal with a Gaussian spectral profile, we derive an analytic expression for the standard deviation of the measurement error for each arrangement. They are then compared with the ideal spectral analyzer (ISA) and with the double-edge Fabry-Perot (DFP) in the case of a shot-noise-limited signal. The DMZ measurement error is shown to be only 1.65 times that of the ISA and is 1.4 times lower than that given by the DFP. The QMZ arrangement provides a measurement that is insensitive to the aerosol scattering contribution but gives a measurement error that is 1.4 times higher than that of the DMZ.

Airborne Lidar LEANDRE II for Water-Vapor Profiling in the Troposphere. I. System description.

The airborne differential absorption lidar LEANDRE II, developed for profiling tropospheric water-vapor mixing ratios, is described. The emitter is a flash-lamp-pumped alexandrite laser, which operates in a double-pulse, dual-wavelength mode in the 727-736 nm spectral domain. Two 50-mJ successive on-line and off-line pulses with an output linewidth of 2.4 x 10(-2) cm(-1) and a spectral purity larger than 99.99% are emitted at a 50-mus time interval. The spectral positioning is controlled in real time by a wavemeter with an absolute accuracy of 5 x 10(-3) cm(-1). The receiver is a 30-cm aperture telescope with a 3.5-mrad field of view and a 1-nm filter bandwidth. These instrument characteristics are defined for measuring the water-vapor mixing ratio with an accuracy better than 0.5 g kg(-1) in the first 5 km of the atmosphere with a range resolution of 300 m, integration on 100 shots, and an instrumental systematic error of less than 2%. The sensitivity study and first results are presented in part II [Appl. Opt. 40, 3462-3475 (2001)].

Airborne Lidar LEANDRE II for Water-Vapor Profiling in the Troposphere. II. First results.

The airborne lidar LEANDRE II, described in part I [Appl. Opt. 40, 3450-3461 (2001)], has been flown on the French Atmospheric Research Aircraft to perform lower-troposphere (0-3.5-km) measurements of the water-vapor mixing ratio. We present and discuss the method used for retrieval of the water-vapor mixing ratio and analyze systematic and random measurement errors in relation to instrument design and performance. The results of a series of test flights are presented. With a 0.8-km horizontal resolution and a 300-m vertical resolution, the standard deviation of the measurement error ranges from approximately 0.05 g kg(-1) at 3.5 km to 0.3-0.4 g kg(-1) near the ground, in agreement with the predicted random error. Comparisons with dew-point hygrometer measurements show a vertically averaged difference of ?0.15 g kg(-1), approximately equal to the observed water-vapor variability.

Identification and disruption of a plant shaker-like outward channel involved in K+ release into the xylem sap.

SKOR, a K+ channel identified in Arabidopsis, displays the typical hydrophobic core of the Shaker channel superfamily, a cyclic nucleotide-binding domain, and an ankyrin domain. Expression in Xenopus oocytes identified SKOR as the first member of the Shaker family in plants to be endowed with outwardly rectifying properties. SKOR expression is localized in root stelar tissues. A knockout mutant shows both lower shoot K+ content and lower xylem sap K+ concentration, indicating that SKOR is involved in K+ release into the xylem sap toward the shoots. SKOR expression is strongly inhibited by the stress phytohormone abscisic acid, supporting the hypothesis that control of K+ translocation toward the shoots is part of the plant response to water stress.

Modeling of Tm, Ho:YAG and Tm, Ho:YLF 2- mum Lasers and Calculation of Extractable Energies.

The development of a model for 2-mum laser operation in Tm, Ho:YAG and YLF crystals pumped in the near infrared is reported. This model, based on a simplified spectroscopic scheme, is fitted to a set of characterization experiments by means of three adjustable parameters. Results show that the excited-state populations are predicted with a relative accuracy of approximately 10% for a large range of pump levels. Using this model, we calculate the extractable energy on short-laser-pulse interactions for the two materials under different operation conditions. We study the sensitivity to pump duration and the optimization of dopant concentrations. We investigate the improvement of the extractable-energy efficiency with multiple-pulse operation. For double-pulse operation the improvement is approximately a factor of 1.5 and leads to overall extractable-energy efficiencies of 16% in YAG and 15% in YLF for an absorbed pump energy of 10 J cm(-3).

A genetic and physical map of bovine chromosome 3.

This paper reports a map of nine polymorphic microsatellite markers previously assigned to bovine chromosome 3 (BTA3) by somatic cell genetics. The linkage group covers 101 cM on the chromosome with an average intermarker distance of 13.9 cM. One marker (INRA200) was isolated from a peak of flow sorted chromosomes 2 and 3. Another marker (INRA197) was derived from a cosmid. The localization of the cosmid by in situ hybridization enabled the orientation of the linkage group on BTA3. Markers were relatively evenly spaced and consequently can be used to complement other mapping data about this chromosome. This establishes a framework of polymorphic markers that can be used to search for quantitative trait loci (QTL).

Injection-seeded pulsed alexandrite laser for differential absorption lidar application.

We describe a Q-switched alexandrite laser injection seeded with a cw single-mode titanium-sapphire laser. The reported experimental results show that this system meets the frequency stabilization required for differential absorption lidar measurement of humidity, pressure, and temperature. The emission of the cw titanium-sapphire master oscillator is locked to an atmospheric absorption line by means of a servoloop with derivative spectroscopy. The spectral position is stabilized within ±3.5 × 10(-4) cm(-1) (10 MHz) of the peak of the line over 1 hr. The alexandrite laser emits pulses of 30 mJ in 500 ns, with a spectral linewidth of ≈ 3.3 × 10(-3) cm(-1) (100 MHz). The position of the centroid of the emitted spectrum has a standard deviation of 6 × 10(-4) cm(-1) (18 MHz) and is held within ±1.3 × 10(-3) cm(-1) (40 MHz) of the peak of the absorption line over 1 h.

Double-pulse dual-wavelength alexandrite laser for atmospheric water vapor measurement.

We describe a new alexandrite laser source arrangement designed to measure atmospheric water vapor using the differential absorption lidar technique. This laser is capable of emitting two pulses at two appropriately selected wavelengths within a single flash lamp discharge. A narrow spectral linewidth of Deltalambda < 1 pm is obtained for each pulse by intracavity filtering with a birefringent filter and two Fabry-Perot interferometers. Wavelength commutation between the two pulses is performed by electro-optically tuning the birefringent filter. The temporal separation between the two pulses can be chosen between 50 and 70 micros and each pulse duration is <250-ns (full width at half-maximum). Typical output energies of 50 mJ/pulse at each wavelength are obtained with this laser system at a 10-Hz repetition rate for a 1.3-kW input electrical power.

[Comparative activity of sulfamethoxazole-trimethoprim (SMZ-TMP) on bacteria responsible for ORL infections]. / Activité comparée du sulfaméthoxazole-triméthoprime (SMZ-TMP) sur des bactéries responsables des infections de la sphère ORL.

The bacteriostatic activity in vitro of co-trimoxazole (SMZ-TMP); ampicillin, tetracycline and oleandomycin was evaluated against 225 bacterial strains. All the strains (49 Pneumococci, 49 Haemophilus, 41 Streptococci and 86 Staphylococcus aureus) were isolated from sinusitis (63 strains) and otitis (162 strains) in monomicrobial samples. The minimal inhibitory concentrations (MICs) of the 4 antibiotics were determined by the agar diffusion method on all strains. Moreover MICs of SMZ-TMP were determined by the broth dilution method on Haemophilus strains. Seventy seven p. cent of the strains were found sensitive to SMZ-TMP, 70.7% to ampicillin, 85.4% to tetracycline and 73% to oleandomycin. SMZ-TMP was the most effective on Staphylococcus aureus (80% of the isolates were sensitive), whereas ampicillin was effective on all Streptococcus, and Pneumococcus strains and tetracycline was the most active on Haemophilus strains (88% of the isolates were sensitive).