Agrobiodiversity-Based Landscape Design in Urban Areas.

Agrobiodiversity represents a system of biological organisms that contribute to agri-food production. In a context marked by a significant loss of food-relevant species and a reduction in their genetic diversity, the adoption of strategies to preserve and enhance the diversity of genetic resources that support and complement agricultural production has become a global challenge. Many sustainable development strategies outlined in recent years directly and indirectly attribute a crucial role to agrobiodiversity in meeting food needs, ensuring food system security, promoting food justice, and enhancing well-being in modern living environments. This contribution aims to analyze the process of knowledge and awareness that has led many cities to plan their urban development by investing in the agricultural matrix and to address the design of open spaces with agricultural biodiversity.

Ecophysiological Response of Vitis vinifera L. in an Urban Agrosystem: Preliminary Assessment of Genetic Variability.

Urban agriculture is an emerging challenge. Identifying suitable agrosystems that allow for the multiple functions of urban agriculture represents a key issue for the reinforcement of the agricultural matrix in cities, with the aims of counteracting and adapting to climate change and providing economic and social benefits. This study aims to produce a preliminary assessment of the adaptability of Italian native and non-native Vitis vinifera L. cultivars to the stressors of an urban environment. The investigation was carried out on the grapevine collection of the Botanical Garden of Rome ("Vigneto Italia"). A total of 15 grapevine varieties were selected for the evaluation of leaf chlorophyll content, stomatal conductance, and chlorophyll fluorescence under abiotic conditions during the growing season of 2021. Spectral signatures were collected from mature leaves, and several vegetation indices (LWI, MCARI, and WBI) were calculated. Our preliminary results highlighted differences in the behavior of the grapevine cultivars. The native ones showed a medium-high level for leaf chlorophyll content (greater than 350 mol m−2), good photosynthetic efficiency (QY > 0.75), and optimal stomatal behavior under drought stress (200 > gs > 50 mmol H2O m−2 s−1). The data allowed for the classification of the tested genotypes based on their site-specific resistance and resilience to urban environmental conditions. The grapevine proved to be a biological system that is highly sensitive to climate variables, yet highly adaptable to limiting growing factors.

Prevalence and Clinical Features of Celiac Disease in a Cohort of Italian Children with Autism Spectrum Disorders.

BACKGROUND: Autism spectrum disorders (ASD) are a heterogeneous group of neurodevelopmental conditions whose etiopathogenesis derives from a complex interaction between genetic liability and environmental factors. In this framework, mounting evidence suggests that immune system dysfunction could be a risk factor contributing to the development of ASD in at least a subpopulation of individuals. In particular, some studies suggest an association between celiac disease (CD)-a long-term autoimmune disorder that primarily affects the small intestine triggered by the ingestion of gluten-and ASD, while others hypothesized a random link. This investigation aimed to evaluate the prevalence of CD in a large sample of school-aged children with ASD and to characterize their clinical profile. METHODS: Medical records of 405 children with ASD aged 5-11 years (mean age: 7.2 years; SD: 1.8 years) consecutively referred to a tertiary-care university hospital between January 2014 and December 2018 were reviewed; among them, 362 had carried out serological testing for CD. RESULTS: Nine patients with positive CD serology were identified, eight of which satisfied the criteria for CD diagnosis. The estimated CD prevalence in ASD children was 2.18% (95% CI, 0.8-3.7), which was not statistically different (1.58%; p = 0.36) from that of an Italian population, matched for age range, considered as a control group (95% CI, 1.26-1.90). Three out of the eight ASD patients with CD did not have any symptoms suggestive of CD. CONCLUSIONS: Our findings did not show a higher prevalence of CD in ASD children than in the control population, but could suggest the utility of routine CD screening, given its frequent atypical clinical presentation in this population.

Response of Olive Shoots to Salinity Stress Suggests the Involvement of Sulfur Metabolism.

Global warming has two dangerous global consequences for agriculture: drought, due to water scarcity, and salinization, due to the prolonged use of water containing high concentrations of salts. Since the global climate is projected to continue to change over this century and beyond, choosing salt-tolerant plants could represent a potential paramount last resort for exploiting the secondary saline soils. Olive is considered moderately resistant to soil salinity as compared to other fruit trees, and in the present study, we investigated the influence of NaCl solutions (ranging from 0 to 200 mM) in a salt-tolerant (cv Canino) and two of its transgenic lines (Canino AT17-1 and Canino AT17-2), overexpressing tobacco osmotin gene, and in a salt-sensitive (Sirole) olive cultivar. After four weeks, most of the shoots of both Canino and Sirole plants showed stunted growth and ultimate leaf drop by exposure to salt-enriched media, contrary to transgenic lines, that did not show injuries and exhibited a normal growth rate. Malondialdehyde (MDA) content was also measured as an indicator of the lipid peroxidation level. To evaluate the role of the S assimilatory pathway in alleviating the adverse effects of salt stress, thiols levels as well as extractable activities of ATP sulfurylase (ATPS) and O-acetyl serine(thiol)lyase (OASTL), the first and the last enzyme of the S assimilation pathway, respectively, have been estimated. The results have clearly depicted that both transgenic lines overexpressing osmotin gene coped with increasing levels of NaCl by the induction of S metabolism, and particularly increase in OASTL activity closely paralleled changes of NaCl concentration. Linear correlation between salt stress and OASTL activity provides evidence that the S assimilation pathway plays a key role in adaptive response of olive plants under salt stress conditions.

Using UAV-based remote sensing to assess grapevine canopy damage due to fire smoke.

BACKGROUND: Bushfires are becoming an increasing issue for the wine sector due to grape and vine losses and smoke taint in wine. Smoke affects vine physiology and the smoke's volatile phenols are absorbed by plants and berries, contaminating the wine. Our hypothesis was that, for the first time, unmanned aerial vehicle (UAV)-based visible images can be used to study the physiology of smoke-affected vines and to assess compromised vines. RESULTS: Procanico vines were exposed to two smoke treatments, a week apart. Gas exchanges and leaf biochemical traits were measured in the short term (30 min after smoke exposure) and in the long term (24 h after smoke exposure). Canopy damage was assessed with conventional vegetation indices (VIs) and by an innovative index derived by UAV-based visible images, the Canopy Area Health Index (CAHI). Gas exchange showed a reduction after the first smoke exposure, but the vines recovered within 24 h. The second smoke exposure led to an irreversible reduction in functional parameters. The VIs exhibited significant differences and CAHI presented a damage gradient related to bushfire nearby. CONCLUSION: The vineyard damage assessment by UAV-based visible images may represent a tool to study the physiological activity of smoke-affected vines and to quantify the loss of destroyed or damaged vines. © 2020 Society of Chemical Industry.

Autonomic Nervous System Response during Light Physical Activity in Adolescents with Anorexia Nervosa Measured by Wearable Devices.

Anorexia nervosa (AN) is associated with a wide range of disturbances of the autonomic nervous system. The aim of the present study was to monitor the heart rate (HR) and the heart rate variability (HRV) during light physical activity in a group of adolescent girls with AN and in age-matched controls using a wearable, minimally obtrusive device. For the study, we enrolled a sample of 23 adolescents with AN and 17 controls. After performing a 12-lead electrocardiogram and echocardiography, we used a wearable device to record a one-lead electrocardiogram for 5 min at baseline for 5 min during light physical exercise (Task) and for 5 min during recovery. From the recording, we extracted HR and HRV indices. Among subjects with AN, the HR increased at task and decreased at recovery, whereas among controls it did not change between the test phases. HRV features showed a different trend between the two groups, with an increased low-to-high frequency ratio (LF/HF) in the AN group due to increased LF and decreased HF, differently from controls that, otherwise, slightly increased their standard deviation of NN intervals (SDNN) and the root mean square of successive differences (RMSSD). The response in the AN group during the task as compared to that of healthy adolescents suggests a possible sympathetic activation or parasympathetic withdrawal, differently from controls. This result could be related to the low energy availability associated to the excessive loss of fat and lean mass in subjects with AN, that could drive to autonomic imbalance even during light physical activity.

Assessing Impacts of Climate Change on Phenology and Quality Traits of Vitis vinifera L.: The Contribution of Local Knowledge.

Developing adaptation strategies in Vitis vinifera, a crop sensitive to climate change, is crucial for resilience of traditional viticultural systems, especially in climate-vulnerable areas like the Mediterranean basin. A progressive warming is demonstrated to alter the geographical distribution of grapevine, reducing land capability for typical grapes and vine productions in most Southern European districts traditionally specialized in tree crops. Grapevine growth and reproduction under climate change require a continuous monitoring to adapt agronomic practices and strategies to global change. The present study illustrates an empirical approach grounded on a set of bio-physical indicators assessing the genotype-related response to climate variation. This approach was tested in Umbria, central Italy, to verify the response of some major international and local grapevine varieties to climate variation during a relatively long time interval (1995-2015). Long-term data for ripening time and berry quality collected in the study area were correlated to representative bioclimatic indices including Winkler, Huglin, and Cool night indicators. Results of this study highlighted the increase of air temperature (reflecting the inherent growth in thermal availability for maturation) and the alteration of precipitation patterns toward more intense precipitation. Climate variability exerted distinctive impacts on grapevine phenology depending on the related genotype. Empirical findings underline the usefulness of a permanent field monitoring of the relationship between selected climate variables and grape ripening with the aim to develop adaptive viticultural practices at farm's scale.

Excessive physical activity in young girls with restrictive-type anorexia nervosa: its role on cardiac structure and performance.

PURPOSE: The aim of this study was to investigate the influence of hyperactivity on left ventricular mass (LVM) in Anorexia Nervosa restricting-type (AN-R) and the correlation between LVM and auxologic parameters/circulating hormones. METHODS: Echocardiography was performed in 44 AN-R girls, subgrouped in 24 hyperactive (ANH+) and 20 non-hyperactive (ANH-), and in 20 controls (HC). LVM indexed to Body Surface Area (LVMi) and LVM indexed to height (LVMh) were calculated. RESULTS: LVMi and LVMh were significantly lower in the AN-R subjects compared to HC. Moreover, both LVMi and LVMh were higher in the ANH+ than in the ANH-. In the HC, LVMi was higher when compared to the ANH- subjects than to the ANH+. Stepwise analysis revealed that in the ANH+ group, fT4 was the only independent predictor of LVMh, while in the ANH- group, height was the only independent predictors of LVMi. CONCLUSIONS: Despite its negative influence on disease severity and outcome, hyperactivity from the standpoint of cardiac function makes the LVM of AN-R young girls more similar to HC. LEVEL OF EVIDENCE: Level III, case-control study.

Serological screening for Celiac Disease in 382 pre-schoolers with Autism Spectrum Disorder.

BACKGROUND: Recent investigations suggest a possible common genetic background between Autism Spectrum Disorders (ASD) and Celiac Disease (CD). However, studies regarding this association are scarce and often limited by the small sample sizes and/or large heterogeneity among ASD groups in terms of demographic and clinical features. The present study aims to investigate the overall CD prevalence (biopsy proven-CD patients plus screening detected tTG and EMA positive cases) in a large population of pre-schoolers with ASD referred to a tertiary care University Hospital. METHODS: We retrospectively collected data about 382 children (mean age: 46.97 ± 13.55 months; age-range: 18-72 months) consecutively diagnosed as ASD (according to the Diagnostic and Statistical Manual of Mental Disorders 4th edition criteria) over the period 2010-2013, and who performed a serological CD screening. RESULTS: The overall CD prevalence was 2.62%, which is statistically significant higher to that reported in the Italian paediatric population (p = 0.0246). Half of these children had no symptoms or risk factors related to CD when they performed the serological screening. CONCLUSIONS: If replicated, these data suggest the importance of regular screening for CD in young patients with ASD, and are of relevance for clinical and public health.

The role of wearable sensors and wireless technologies for the assessment of heart rate variability in anorexia nervosa.

PURPOSE: Bradycardia and abnormal cardiac autonomic function are related to increased mortality in anorexia nervosa (AN). The aim of this study was to assess heart rate (HR) and HR variability of young adolescents with AN as compared to controls by means of wearable sensors and wireless technologies. METHOD: The ECG signal was recorded in 27 AN girls and 15 healthy girls at rest using a wearable chest strap. The tachogram, the mean intervals between R peaks (meanRR), the root mean square of successive differences (RMSSD), the power of low-frequency (LF) and high-frequency (HF) bands and the LF/HF ratio were assessed. RESULTS: All AN girls showed a reduced HR and an increased meanRR and RMSSD. An HF increase, a LF decrease, and a LF/HF reduction indicated a prevalence of the parasympathetic on sympathetic activity. CONCLUSIONS: The instruments used in this pilot study were feasible, unobtrusive and extremely suitable in AN subjects who are burdened by high incidence of cardiovascular mortality; their application could open to new approaches of vital signs monitoring in hospitals as well as in home settings.

Selective cognitive empathy deficit in adolescents with restrictive anorexia nervosa.

BACKGROUND: A growing, but conflicting body of literature suggests altered empathic abilities in subjects with anorexia nervosa-restricting type (AN-R). This study aims to characterize the cognitive and affective empathic profiles of adolescents with purely AN-R. METHODS: As part of a standardized clinical and research protocol, the Interpersonal Reactivity Index (IRI), a valid and reliable self-reported instrument to measure empathy, was administered to 32 female adolescents with AN-R and in 41 healthy controls (HC) comparisons, matched for age and gender. Correlational analyses were performed to evaluate the links between empathy scores and psychopathological measures. RESULTS: Patients scored significantly lower than HC on cognitive empathy (CE), while they did not differ from controls on affective empathy (AE). The deficit in CE was not related to either disease severity nor was it related to associated psychopathology. CONCLUSION: These results, albeit preliminary, suggest that a dysfunctional pattern of CE capacity may be a stable trait of AN-R that should be taken into account not only for the clinical management, but also in preventive and therapeutic intervention.

Low-dose olanzapine monotherapy in girls with anorexia nervosa, restricting subtype: focus on hyperactivity.

OBJECTIVE: The aim of this study was to evaluate the efficacy of olanzapine in girls with anorexia nervosa, restricting subtype (ANr). METHODS: Thirteen patients (mean age 13.7 +/- 2.3 years, age range 9.6-16.3 years) enrolled in a multimodal treatment for ANr were evaluated with standardized measures at baseline and after 1 and 6 months after starting low-dose olanzapine monotherapy (mean dose 4.13 mg/day). RESULTS: A significant improvement was evident on weight (body mass index, BMI), global functioning (Children's Global Assessment Scale, CGAS), eating attitudes (Eating Attitudes Test-26, EAT-26), anxious-depressive symptoms (Child Behavior Checklist, CBCL) and hyperactivity (Structured Inventory for Anorexic and Bulimic Syndromes, SIAB). At the end of the 6-month follow up, 7 patients were responders according to an improvement of at least 50% in the EAT-26 results. The only measure that improved significantly in responders, but not in nonresponders, was hyperactivity (SIAB). Clinical improvement, in terms of both body mass index (BMI) recovery and global functioning, paralleled the improvement of hyperactivity, was evident at the end of the first month of treatment, and further increased in the following 5 months, with minimal side effects. CONCLUSIONS: Low-dose olanzapine monotherapy may be useful as adjunctive treatment of youths with ANr. It is suggested that efficacy may be mediated by a decrease of hyperactivity.

Analysis of quantitative ultrasound graphic trace and derived variables assessed at proximal phalanges of the hand in healthy subjects and in patients with cerebral palsy or juvenile idiopathic arthritis. A pilot study.

Amplitude-dependent speed of sound (AD-SoS) and bone transmission time (BTT) are the quantitative ultrasound (QUS) variables usually assessed at proximal phalanges of the hand to estimate bone mineral status. The aim of the study was to provide a reference database for some additional QUS variables reflecting morphology of the ultrasound graphic trace according to gender, age, height, weight, and body mass index (BMI), and to assess their clinical usefulness. Fifty-two patients (age 3.1-20.9 years) affected by cerebral palsy with spastic tetraplegia (CPST, n=38) or polyarticular active juvenile idiopathic arthritis (JIA, n=14) were examined. In addition to AD-SoS and BTT, two QUS variables derived from the morphological analysis of ultrasound graphic trace, such as energy, extrapolated from the area under the ultrasound signal received, and weighted-slope (W-slope), derived from the angular coefficient of the regression line fitting the top point of the peaks of the ultrasound signal, were measured by phalangeal QUS (DBM Sonic, IGEA). The values of all the QUS variables measured in the patients were compared with our own sex- and age-reference values (n=1083, 587 males and 496 females, aged 3-21 years). The mean values of AD-SoS, BTT, energy, and W-slope were reduced (P<0.0001) in patients as a whole compared with normative data (-2.4+/-1.2, -2.7+/-1.5, -2.5+/-1.1, -2.5+/-1.1 Z-score, respectively). Fractured patients showed lower (P<0.001-P<0.0001) values of the QUS variables than fracture-free patients (AD-SoS, -3.3+/-1.2 and -1.8+/-0.9; BTT, -3.9+/-1.7 and -1.8+/-1.1; energy, -3.2+/-1.2 and -2.2+/-0.7; W-slope, -3.4+/-1.4 and -2.2+/-0.9 Z-score, respectively). There was no difference (P=NS) between patients with CPST and those with JIA. Age and height were positively correlated with all the QUS variables (r=0.55-0.79, P<0.01-P<0.0001). QUS variables were positively correlated among them (r=0.74-0.94, P<0.0001). Age and number of fractures were independent predictors of the QUS variables (coefficients: AD-SoS, 11.466 and -17.642; BTT, 0.049 and -0.045; energy, 1.072 and -1.303; W-slope, 0.046 and -0.067; respectively). In conclusion, measurement of QUS variables derived from the morphological analysis of the ultrasound signal could give additional information in estimating bone mineral status in children and adolescents, probably reflecting some aspect related to bone structure.

Docking and molecular dynamics simulation of the Azurin-Cytochrome c551 electron transfer complex.

We coupled protein-protein docking procedure with molecular dynamics (MD) simulation to investigate the electron transfer (ET) complex Azurin-Cytochrome c551 whose transient character makes difficult a direct experimental investigation. The ensemble of complexes generated by the docking algorithm are filtered according to both the distance between the metal ions in the redox centres of the two proteins and to the involvement of suitable residues at the interface. The resulting best complex (BC) is characterized by a distance of 1.59 nm and involves Val23 and Ile59 of Cytochrome c551. The ET properties have been evaluated in the framework of the Pathways model and compared with experimental data. A 60 ns long MD simulation, carried on at full hydration, evidenced that the two protein molecules retain their mutual spatial positions upon forming the complex. An analysis of the ET properties of the complex, monitored at regular time intervals, has revealed that several different ET paths are possible, with the occasional intervening of water molecules. Furthermore, the temporal evolution of the geometric distance between the two redox centres is characterized by very fast fluctuations around an average value of 1.6 nm, with periodic jumps at 2 nm with a frequency of about 70 MHz. Such a behaviour is discussed in connection with a nonlinear dynamics of protein systems and its possible implications in the ET process are explored.

Denial of symptoms and psychopathology in adolescent anorexia nervosa.

OBJECTIVE: To evaluate the psychopathological status and denial of symptoms in a sample of 38 consecutively admitted adolescents with anorexia nervosa (AN). METHOD: The Schedule for Affective Disorders and Schizophrenia for School-Aged Children was used to determine the categorical diagnosis of eating disorder. The anorexic adolescents completed the EAT-40 (Eating Attitude Test) and, on the basis of its score, the sample was dichotomized in a false-negative group (FNG) scoring under 30 and a positive group (PG) scoring over 30. We compared these two subgroups in terms of clinical variables (age of onset and admission, duration of illness prior to admission, diagnosis, BMI) and psychopathology assessed by the CBCL (Child Behaviour Checklist) and the YSR (Youth Self-Report). In order to evaluate the possible role of diagnosis and BMI on the EAT score we also dichotomized the sample with respect to the diagnosis (full vs. partial AN) and to the mean female BMI (< or = 15 vs. > 15). RESULTS: A significant difference was found in terms of duration of illness prior to admission, which was briefer in the FNG. Higher CBCL and YSR values were found in the PG with significant differences in terms of YSR internalizing symptoms. A large amount of significantly positive Pearson's correlations were found between the CBCL and YSR values in the FNG. No significant Pearson's correlations were found between EAT, BMI and diagnosis. CONCLUSIONS: The lower CBCL and YSR values in the FNG seem to point out a tendency of this group to deny anxiety and depression as well as an eating pathology; the longer duration of illness prior to admission in the PG seems to support the hypothesis that the PG may be considered to be not so much more disturbed as more aware of its eating attitudes and psychopathology. The nature of denial in anorexic adolescents is discussed.