RESUMEN
In habitats where stressors are frequent or persistent, it can become increasingly difficult for wildlife to appropriately match their endocrine responses to these more challenging environments. The dynamic regulation of glucocorticoid (GC) hormones plays a crucial role in determining how well individuals cope with environmental changes. Amphibians exposed to agricultural stressors can dampen aspects of their GC profile (baseline, agitation, recovery, stress responsiveness, and negative feedback) to cope in these stressful environments, but this dampening can lead to reductions in an individual's reactive scope and a loss of endocrine flexibility. Organic agriculture could potentially limit some of these effects, however, little is known about how amphibians respond physiologically to organic agricultural environments. We compared GC profiles of Hyla versicolor tadpoles from three treatments: natural ponds (<5% agriculture within 500m), ponds near organic farms, and ponds near conventional farms. We hypothesized that tadpoles would cope with agricultural habitats by dampening stress responsiveness and exhibiting more efficient negative feedback and that the magnitude of these changes in response would differ based on agricultural method. We found that tadpoles from conventional and organic ponds were less likely to downregulate GCs via negative feedback after stressor exposure than tadpoles from natural ponds. For agricultural tadpoles that did downregulate GCs after the stressor, we found lower stress responsiveness and faster downregulation to baseline corticosterone than tadpoles from natural ponds. These results point to an accumulation of wear-and-tear, leading to an overall reduction in reactive scope and limited GC flexibility in our agricultural tadpoles. Regardless of agricultural method used, agricultural tadpoles exhibited the same patterns of GC response, indicating that current efforts to incentivize farmers to switch to organic farming methods may not be sufficient to address negative agricultural impacts on amphibians.
RESUMEN
Invasive fish predators are an important factor causing amphibian declines and may have direct and indirect effects on amphibian survival. For example, early non-lethal exposure to these stressors may reduce survival in later life stages, especially in biphasic species. In amphibians, the glucocorticoid hormone corticosterone is released by the hypothalamo-pituitary-interrenal axis (HPI), as an adaptive physiological response to environmental stressors. The corticosterone response (baseline and response to acute stressors) is highly flexible and context dependent, and this variation can allow individuals to alter their phenotype and behavior with environmental changes, ultimately increasing survival. We sampled larvae of the spring salamander (Gyrinophilus porphyriticus) from two streams that each contained predatory brook trout (Slavelinus fontinalis) in the lower reaches and no predatory brook trout in the upper reaches. We measured baseline and stress-induced corticosterone release rates of larvae from the lower and upper reaches using a non-invasive water-borne hormone assay. We hypothesized that corticosterone release rates would differ between larvae from fish-present reaches and larvae from fish-free reaches. We found that baseline and stressor-induced corticosterone release rates were downregulated in larvae from reaches with fish predators. These results indicate that individuals from reaches with predatory trout are responding to fish predators by downregulating corticosterone while maintaining an active HPI axis. This may allow larvae more time to grow before metamorphosing, while also allowing them to physiologically respond to novel stressors. However, prolonged downregulation of corticosterone release rates can impact growth in post-metamorphic individuals.
