RESUMEN
OBJECTIVE: To determine the incidence and occupational variation of granulosa cell tumours (GCTs) in Finland, Iceland, Norway and Sweden over a 60-year period, 1953-2012. DESIGN: A longitudinal cohort study. SETTING AND POPULATION: Finland, Iceland, Norway and Sweden and a total of 249 million women over a 60-year period (1953-2012). The NOCCA (Nordic Occupational Cancer Study) included 6.4 million women with 776 incident GCT cases diagnosed until the end of follow up. METHODS: Incidence rates were calculated from the national cancer registries and compared using quasi-Poisson regression models. Occupation-specific standardised incidence ratios (SIRs) were calculated from the Nordic Occupational Cancer (NOCCA) database. MAIN OUTCOME MEASURES: Incidence rates and standardised incidence ratios. RESULTS: The age-adjusted (World Standard) incidence rates remained quite constant: about 0.6-0.8 per 100 000 for most of the study period. The age-specific incidence was highest at 50-64 years of age. There were no occupations with significantly increased risk of GCT. Major changes in the use of oral contraceptives, postmenopausal hormonal therapy, fertility rate and lifestyle in general during the study period and among different occupational categories do not appear to have a marked effect on the incidence of GCT. CONCLUSION: Our findings support the concept of GCT as a primarily sporadic, not exposure-related, cancer. TWEETABLE ABSTRACT: The Nordic incidence rates of GCTs show stability over time and among different occupational categories.
Asunto(s)
Tumor de Células de la Granulosa/epidemiología , Enfermedades Profesionales/epidemiología , Exposición Profesional/efectos adversos , Neoplasias Ováricas/epidemiología , Adulto , Estudios de Cohortes , Femenino , Finlandia/epidemiología , Estudios de Seguimiento , Humanos , Islandia/epidemiología , Incidencia , Estudios Longitudinales , Persona de Mediana Edad , Noruega/epidemiología , Ocupaciones , Sistema de Registros , Medición de Riesgo , Factores de Riesgo , Suecia/epidemiologíaRESUMEN
The content and the distribution of the glycoconjugate sugar residues were investigated in the human umbilical cord from normally grown pregnancies and from pregnancies complicated by intra-uterine growth retardation with absent or reversed flow in the umbilical artery. The gestational age range was 29-41 weeks. For this purpose, a battery of eight horseradish peroxidase conjugated lectins was used (ConA, WGA, PNA, SBA, DBA, LTA, UEA I, and OOA; see text). Our data showed that in both study groups ConA, WGA and PNA (after neuraminidase digestion) and LTA, UEA I, and OOA reacted with Wharton's jelly and vessels of the umbilical cord from the first weeks of gestation considered. SBA reactivity was observed only in the amniotic epithelial cells of the jelly and in the endothelial cells of the vessels. The reactivity with the most part of the lectins decreased or was lacking in the various components of the umbilical cord between 39 and 41 weeks of gestation in the normal group and between 36 and 37 weeks of gestation in the pathological one. Moreover, the intensity of reactivity with ConA, WGA and PNA (after neuraminidase digestion) and LTA, UEA I, and OOA in the normal group and with PNA (before and after neuraminidase digestion) and LTA and OOA in the pathological one was stronger in the peripheral portion of the jelly with respect to the adventitial one. The reactivity with ConA and UEA I was stronger in the vein than in the arteries in both study groups. Some hypotheses are reported on the role played by the sugar residues in the normal and pathological umbilical cords.
Asunto(s)
Retardo del Crecimiento Fetal/fisiopatología , Lectinas/metabolismo , Arterias Umbilicales/fisiopatología , Cordón Umbilical/metabolismo , Concanavalina A/metabolismo , Diástole , Femenino , Edad Gestacional , Histocitoquímica , Humanos , Maclura/química , Neuraminidasa/metabolismo , Aglutinina de Mani/metabolismo , Lectinas de Plantas/metabolismo , Embarazo , Venas Umbilicales/metabolismo , Aglutininas del Germen de Trigo/metabolismoRESUMEN
The glycoconjugates sugar residues content, distribution and changes in the enterocytes of different tracts of the developing intestine of the chick embryo and of 1-day-old chick were investigated, using a battery of seven HRP-conjugated lectins (DBA, SBA, PNA, WGA, ConA, LTA and UEAI). The results of the present research have shown the presence of a large amount of glycoconjugates sugar residues in the enterocytes of duodenal, ileal and colonic anlage, starting from the beginning of the second week of incubation. Differences were detected among the three investigated intestinal segments, as to the time of appearance of the glycoconjugates sugar residues in the enterocytes. The duodenal enterocytes showed the most precocious appearance of lectin-reactive material, followed by the ileal enterocytes and afterwards by colonic enterocytes. The duodenal enterocytes were characterised by the presence of SBA binding sites, which were not detectable in the duodenal enterocytes of the adult animal.
Asunto(s)
Envejecimiento/metabolismo , Diferenciación Celular/fisiología , Pollos/crecimiento & desarrollo , Pollos/metabolismo , Sistema Digestivo/crecimiento & desarrollo , Sistema Digestivo/metabolismo , Enterocitos/metabolismo , Glicoconjugados/metabolismo , Animales , Animales Recién Nacidos , Sitios de Unión/fisiología , Compartimento Celular/fisiología , Embrión de Pollo , Pollos/anatomía & histología , Colon/citología , Colon/crecimiento & desarrollo , Colon/metabolismo , Gránulos Citoplasmáticos/metabolismo , Gránulos Citoplasmáticos/ultraestructura , Sistema Digestivo/citología , Duodeno/citología , Duodeno/crecimiento & desarrollo , Duodeno/metabolismo , Enterocitos/citología , Aparato de Golgi/metabolismo , Aparato de Golgi/ultraestructura , Histocitoquímica , Peroxidasa de Rábano Silvestre , Íleon/citología , Íleon/crecimiento & desarrollo , Íleon/metabolismo , Lectinas , Microvellosidades/metabolismo , Microvellosidades/ultraestructuraRESUMEN
It is well known that cell surface glycoconjugates play a determinant role in cellular recognition, cell-to-cell adhesion and serve as receptor molecules. T-lymphocytes are in strict contact with the thymic epithelial cells, which control their process of maturation and proliferation. On the other hand the normal maturation of the epithelial cells is believed to be induced by T-lymphocytes. For these reasons we have studied the glycoconjugates saccharidic moieties of the sessile and motile cells in the thymus of normal male albino Wistar rats and their changes following cyclosporin-A treatment, using a battery of seven HRP-lectins. Cytochemical controls were performed for specificity of lectin-sugar reaction. Some sections were pre-treated with neuraminidase prior to staining with HRP-lectins. Our results have demonstrated, in the control rats, a large amount and a variety of terminal and subterminal oligosaccharides within and/or on the epithelial thymic cells and in macrophages. After cyclosporin-A treatment, among the thymic epithelial cells, the subcapsular, paraseptal and perivascular cells showed the loss of some sugar residues, which characterized the same cells in the intact thymus. Some hypotheses are reported on the role played by the glycoconjugate sugar residues in control and cyclosporin-A treated rats.
Asunto(s)
Metabolismo de los Hidratos de Carbono , Ciclosporina/farmacología , Glicoconjugados/metabolismo , Inmunosupresores/farmacología , Timo/citología , Timo/efectos de los fármacos , Animales , Atrofia , Movimiento Celular/efectos de los fármacos , Movimiento Celular/fisiología , Histocitoquímica , Peroxidasa de Rábano Silvestre , Lectinas , Masculino , Neuraminidasa/química , Ratas , Ratas Wistar , Timo/metabolismo , Fijación del TejidoRESUMEN
The aim of the present research was to investigate the changes of the sugar residues in the oviduct in the course of ageing in postmenopausal women vs normally menstruating women, by means of lectin histochemistry. Twenty asymptomatic postmenopausal women (48-83 years old) were recruited among patients who underwent a vaginal hysterectomy. Eight normally menstruating women were recruited as controls. Fragments of Fallopian tubes (pars ampullaris) were fixed in 10% formalin and routinely processed. The sections were labelled with HRP-lectins (PNA, SBA, DBA, WGA, Con A, LTA, UEAI). Some sections were pre-treated with neuraminidase prior to staining with HRP-lectins. Among the postmenopausal patients, our histochemical data showed that there was no difference in the localization and distribution of sugar residues of glycoconjugates as detected by various HRP-lectins. Moreover, our results demonstrated that the oviductal epithelium is characterized by apical reactivity in both ciliated and non-ciliated cells. In the course of ageing, the ciliated cells changed their morphology from bathyprismatic to large and rounded shape. ConA lectin reacted intensely with such highly degenerating ciliated cells and could be considered a marker of these cells. The degenerating ciliated cells are also characterized by the absence of sialic acid. In comparison with the sugar residues present in the control group, the oviductal epithelium of postmenopausal women is characterized by the loss of reactivity with DBA, WGA and ConA. Moreover, PNA reactive material was present at the free border of the ciliated and non-ciliated cells. The latter findings were statistically confirmed and could be considered strictly related to the ageing process.
Asunto(s)
Trompas Uterinas/crecimiento & desarrollo , Anciano , Anciano de 80 o más Años , Envejecimiento , Metabolismo de los Hidratos de Carbono , Endometrio/anatomía & histología , Endometrio/crecimiento & desarrollo , Trompas Uterinas/anatomía & histología , Trompas Uterinas/metabolismo , Femenino , Histocitoquímica , Peroxidasa de Rábano Silvestre , Humanos , Hidrólisis , Lectinas , Persona de Mediana Edad , Neuraminidasa , Adhesión en Parafina , Posmenopausia/fisiologíaRESUMEN
The oligosaccharidic content, distribution and changes of the glycoconjugates in the testis of the chick embryo, from the 8th day of incubation to hatching, were studied using a battery of seven HRP-conjugated lectins (DBA, SBA, PNA, ConA, WGA, LTA, and UEA I). Our findings showed that ConA and WGA appeared to characterize the spermatogonia during their differentiation, maturation, migration and meiosis. In the Sertoli cells, a change of localization of staining with ConA and WGA was revealed during the differentiation and maturation of these cells. The basal membrane was characterized by the reactivity with ConA and WGA from the early stages of incubation. ConA, WGA and PNA reacted with the endothelial cells of the testis for the whole period of incubation considered. Moreover, the interstitial cells, since their appearance, showed reactivity with ConA, WGA and PNA at the plasma membrane and the cytoplasm.
Asunto(s)
Embrión de Pollo/metabolismo , Glicoconjugados/metabolismo , Lectinas/metabolismo , Oligosacáridos/metabolismo , Testículo/embriología , Animales , Desarrollo Embrionario y Fetal , Técnicas para Inmunoenzimas , MasculinoRESUMEN
A lectin histochemical study was performed to investigate the distribution and changes of the oligosaccharidic component of the glycoconjugates in the lung of chick embryos, of 1-day-old chick, and of the adult animal. For this purpose, a battery of seven horseradish peroxidase-conjugated lectins (PNA, SBA, DBA, WGA, Con A, LTA, and UEA I) were employed. During the first phase of parabronchi and atria formation, D-galactose-(beta1-->3)-N-acetyl-D-galactosamine, beta-N-acetyl-D-galactosamine, D-glucosamine, alpha-D-mannose, and sialic acid, present at the level of the surface and of cytoplasmic granules of the lining epithelial cells, seem to play a role in regulating morphogenetic phenomena. In the subsequent phases, the parabronchial lumen and the atrial cavities were characterized by the presence of lectin-reactive material rich in terminal D-galactose-(beta1-->3)-N-acetyl-D-galactosamine, beta-N-acetyl-D-galactosamine, D-glucosamine and alpha-D-mannose. From day 18 onwards and immediately after hatching, the free border of the cells lining the air capillaries was characterized by the presence of beta-N-acetyl-D-galactosamine and alpha-D-mannose. The appearance of these sugar residues was concomitant with the beginning of respiratory activity.
Asunto(s)
Metabolismo de los Hidratos de Carbono , Embrión de Pollo/fisiología , Glicoconjugados/metabolismo , Envejecimiento/metabolismo , Animales , Animales Recién Nacidos/metabolismo , Embrión de Pollo/anatomía & histología , Embrión de Pollo/metabolismo , Histocitoquímica , Lectinas , Oligosacáridos/metabolismoRESUMEN
The content, distribution and changes of the glycoconjugates sugar residues in the ovaries of chick embryos, from the 8th day of incubation to hatching and in 1-day old chick, were investigated. For this purpose, a battery of seven HRP-conjugated lectins was used (DBA, SBA, PNA, ConA, WGA, LTA and UEA I). Our data showed that SBA was a marker of the most immature oogonia in the ovarian cortex and medulla. The reactivity with ConA appeared to characterize the cells immediately prior to as well as during the meiotic division, as demonstrated by the presence of alpha-D-mannose at the "Balbiani bodies" in the oogonia of the ovarian cortex. The detection of Con A and SBA reactivity corresponded to maturative stages of the early oogonia in different cortical zones of the chick ovary. Our data also revealed that PNA seemed to be a marker of the degenerating oogonia located in the ovarian medulla. Moreover, PNA binding was a characteristic finding in the endothelial cells of the vessels located in the compact portion of the medulla in the left ovary, from the 8th to the 21st day of incubation and after hatching; PNA reactivity was only seen from the 16th day onwards in the endothelial cells of the cortex. During the whole considered period of incubation and after hatching, reactivity with UEAI, LTA and DBA was never detected.
Asunto(s)
Animales Recién Nacidos/metabolismo , Embrión de Pollo/metabolismo , Pollos/metabolismo , Lectinas/metabolismo , Ovario/embriología , Ovario/metabolismo , Animales , Embrión de Pollo/fisiología , Femenino , Histocitoquímica , Ovario/citología , Distribución TisularRESUMEN
The anlage of duodenum, ileum and colon were removed from chick embryos of day 8-21 of incubation and from 1-day-old chicks. A battery of seven different horseradish peroxidase-conjugated lectins (PNA, SBA, DBA, Con A, WGA, LTA and UEAI) was used to study the carbohydrate residues of the glycoconjugates in the goblet cells of the three parts of the intestine. The main results can be summarized as follows: differences in lectin binding were absent in the proximal and distal parts of the duodenum, ileum and colon. Lectin histochemistry showed differences among the three intestinal segments for the time of appearance of the oligosaccharides in the goblet mucus. In the colonic goblet cells of 1-day-old chicks, LTA and UEAI lectins showed two different types of linkage of alpha-L-fucose. This is the first demonstration of UEAI reactive sites in Gallus domesticus.
Asunto(s)
Carbohidratos/análisis , Células Caliciformes/química , Lectinas/análisis , Animales , Animales Recién Nacidos , Embrión de Pollo , Pollos , Colon/química , Colon/embriología , Colon/ultraestructura , Duodeno/química , Duodeno/embriología , Duodeno/ultraestructura , Células Caliciformes/ultraestructura , Íleon/química , Íleon/embriología , Íleon/ultraestructura , Técnicas para InmunoenzimasRESUMEN
It is well known that cell surface glycoconjugates play an important role in cell proliferation, adhesion and differentiation. The aim of this investigation was to define the changes of the glycoconjugate saccharidic moieties in the epidermis and derma of patients affected by several skin pathologies such as seborrheic keratosis, lichen planus, granuloma annulare and palmoplantaris keratoderma. Bioptical specimens from skin lesions as well as from normal skin were fixed in Carnoy's fluid and routinely processed. The sections were treated with HRP-lectins (PNA, DBA, SBA, WGA, ConA, LTA and UEAI). Cytochemical controls were performed for specificity of lectin-sugar reaction. Some sections were pre-treated with neuraminidase prior to staining with HRP lectins. In comparison with normal human skin, epidermal lectin binding pattern in the considered diseases showed considerable qualitative and quantitative variations. In general, in all the considered pathologies, a lack and/or a decrease in lectin binding at the epidermal layers was observed; among the various diseases, differences in cellular localisation of the sugar residues were also noted. In such respect, an exception was represented by seborrheic keratosis, where the cells of the basal layer showed PNA reactivity, which was absent in the basal layer of the normal skin. Although seborrheic keratosis and lichen planus have been studied by others authors, our findings are not in total accordance concerning lectin binding; this is probably due to the different fixatives employed. Our findings seem to reveal significant changes in keratinocyte glycoconjugate oligosaccharides in the previously mentioned diseases, providing clues to their pathogenesis.
Asunto(s)
Granuloma Anular/metabolismo , Queratodermia Palmoplantar/metabolismo , Queratosis Seborreica/metabolismo , Lectinas/metabolismo , Liquen Plano/metabolismo , Oligosacáridos/metabolismo , Lectinas de Plantas , Concanavalina A/metabolismo , Glicoconjugados/metabolismo , Granuloma Anular/patología , Humanos , Queratodermia Palmoplantar/patología , Queratosis Seborreica/patología , Liquen Plano/patología , Aglutinina de Mani/metabolismo , Aglutininas del Germen de Trigo/metabolismoRESUMEN
A lectin histochemical study was performed to investigate the glycoconjugate saccharidic moieties on the endometrial epithelium and stroma in 12 women undergoing controlled ovarian hyperstimulation (COH) for in-vitro fertilisation for embryo transfer (IVF-ET) in early luteal phase. 7 control subjects were also evaluated. For this purpose a battery of seven horseradish peroxidase-conjugated lectins (PNA, SBA, DBA, WGA, ConA, LTA and UEA I) was used. Cytochemical controls were performed for specificity of lectin-sugar reaction. As far as the endometrial glands and stroma are concerned, the obtained data showed no differences in the endometrial lectin binding between the subjects of the control group and the ones undergoing COH, with the exception of PNA reactivity at the level of the apical portion of the glandular cells, which was detected only in COH women. It is noteworthy that, although the endometrial dating using the Noyes's criteria showed marked dissynchronies between the stroma and the glands in COH subjects, a uniformity of lectin binding, revealing the same type and localization of terminal oligosaccharides, was observed in all the examined subjects. The uniformity in distribution of the sugar residues detected in the endometrial specimens following COH might be due to the massive FSH and/or hCG treatment which probably determines an endometrial environment almost equal in all the examined subjects. In all the treated subjects reactivity with sialidase-WGA and ConA, revealing the presence of N-acetyl-D-glucosamine and D-mannose respectively, was detected at the level of the lining epithelium.
Asunto(s)
Endometrio/metabolismo , Lectinas/metabolismo , Lectinas de Plantas , Proteínas de Soja , Adulto , Concanavalina A/metabolismo , Endometrio/patología , Femenino , Fertilización In Vitro , Peroxidasa de Rábano Silvestre/metabolismo , Humanos , Síndrome de Hiperestimulación Ovárica/inducido químicamente , Síndrome de Hiperestimulación Ovárica/metabolismo , Ovario , Aglutinina de Mani/metabolismo , Aglutininas del Germen de Trigo/metabolismoRESUMEN
A battery of horseradish peroxidase-conjugated lectins (PNA, SBA, DBA, WGA, ConA, LTA and UEAI) was used to study the distribution of glycoconjugate sugar residues in the exocrine and endocrine pancreas of chick embryos, 1-day-old chicks and adult animals. During the period of incubation considered here, the time of appearance and changes in the oligosaccharides were noted in the acinar cells, in the endocrine cells of the "light islets" and in the capillary endothelial cells. In the developing exocrine pancreas, the basolateral surface of the acinar cells reacted with PNA, WGA, LTA and ConA for the entire period of incubation while SBA, WGA, LTA and ConA reactivity was detected in the apical plasma membrane. Zymogen granule membrane reacted with PNA, WGA and LTA from the 12th day onward, while ConA reactivity was detectable from the 7th day onward. Owing to its early appearance, alpha-D-mannose, revealed by ConA lectin, might in some way contribute to the maturation of the zymogen granule. In the 1-day-old chick and in the adult the cellular surface reacted with WGA and ConA. The zymogen granule membrane reacted with PNA, WGA and ConA in the 1-day-old chick. In the adult, WGA and ConA reactivity was observed in the zymogen granule membrane, whereas at this site PNA reactivity was revealed only after neuraminidase digestion. The hypothesis formulated by others that granule membrane lectin reactivity is equal to the apical membrane reactivity, owing to exocytotic processes, is not consistent with our results in the chick embryos, the 1-day-old chick and the adults. The surface and the granules of the islet beta cells in different periods of incubation reacted with WGA, SBA and, after neuraminidase treatment, with PNA. The same reactivity was seen also in 1-day-old chick while in the adult the granules of the beta cells also reacted with ConA. These findings show the achievement of an almost adult-like state of glycosylation of the glycoconjugates in the beta cells at an early stages of development.
Asunto(s)
Glicoconjugados/metabolismo , Islotes Pancreáticos/metabolismo , Monosacáridos/metabolismo , Páncreas/metabolismo , Envejecimiento/metabolismo , Animales , Animales Recién Nacidos , Embrión de Pollo , Islotes Pancreáticos/citología , Páncreas/citología , Páncreas/embriologíaRESUMEN
Lectin histochemistry was used to investigate the glycoconjugate saccharidic moieties in the skin of human fetuses ranging in age from the 8th to the 13th week of gestation. For this purpose, seven HRP-conjugated lectins were employed (Con A, WGA, PNA, DBA, SBA, UEAI and LTA). The distribution and changes of the sugar residues of glycoconjugates at the level of the various layers of the fetal epidermis, as well of the underlying mesenchyme were studied. We have shown that UEAI reactivity is a characteristic finding of the cells of the intermediate layer at the 11th and 12th week of gestation. LTA reactivity was detected only at the free border of the peridermal cells from the 10th to the 13th week of gestation. We demonstrated the presence of PNA binding sites at both the basal and peridermal cells from the 8th week of gestation, whereas other authors have stated that the appearance of PNA reactivity coincides with the onset of fetal skin stratification (11th-12th week of gestation). Moreover, some typical features in lectin reactivity at the level of the mesenchymal cells and fibres have been pointed out.
Asunto(s)
Oligosacáridos/metabolismo , Piel/embriología , Concanavalina A , Epidermis/embriología , Epidermis/metabolismo , Fijadores , Edad Gestacional , Peroxidasa de Rábano Silvestre , Humanos , Lectinas , Mesodermo/metabolismo , Aglutinina de Mani , Piel/metabolismoRESUMEN
A lectin histochemical study was performed to investigate the glycoconjugate saccharidic moieties of the human postmenopausal endometrium (14 atrophic and 15 hyperplastic). For this purpose a battery of seven horseradish peroxidase-conjugated lectins (PNA, SBA, DBA, WGA, ConA, LTA and UEA I) was used. No differences in lectin binding between atrophic and hyperplastic endometria were observed. This investigation allowed us to provide a basic picture of the oligosaccharidic distribution in postmenopausal endometria. The data on the saccharidic distribution at the postmenopausal endometria showed a large amount of sugar residues at all the investigated sites, i.e. the lining and glandular epithelium, the stroma and the vessels (capillary and large vessels). Furthermore, at the endometrial lining epithelium, at the glands and at the wall of the blood vessels of some postmenopausal women the presence of alpha-L-fucosyl residues which bind via alpha (1-6) linkage to penultimate glucosaminyl residues and/or difucosylated oligosaccharides was demonstrated for the first time.
Asunto(s)
Carbohidratos/análisis , Endometrio/patología , Oligosacáridos/análisis , Posmenopausia , Anciano , Atrofia , Femenino , Fucosa/análisis , Glucosamina , Histocitoquímica , Humanos , Hiperplasia , Lectinas , Persona de Mediana EdadRESUMEN
Histochemical methods used to investigate the appearance and the changes of the epithelial mucins produced by the lining and glandular epithelium of the chick embryo gizzard from the 7th day of incubation to hatching. Carboxylated, sulfated and neutral mucins appeared simultaneously at the superficial layer of the lining epithelium (7th day), at cells located in the inner part of the lining epithelium surrounding intercellular spaces (9th day) and at the tubular glands (15th day). During the period of incubation considered, an increase and decrease in the production of the various mucins were observed at the lining epithelium. However, a gradual increase of mucous production was detected at the ephitelial cells surrounding intercellular spaces (from the 9th to the 17th day) and at the tubular glands (from the 15th to the 21st day). Moreover, three different overlapping steps of mucous release were observed during the development of the gizzard. The histochemical characteristics and the trend of production of the mucous material in the gizzard during the embryonic development were different when compared with those found in the proventriculus (Sgambati et al., 1995). These differences are probably related to the different function of the mucins in the two parts of the stomach.
Asunto(s)
Molleja de las Aves/química , Molleja de las Aves/embriología , Histocitoquímica , Mucinas/análisis , Azul Alcián , Animales , Embrión de Pollo , Colorantes , Epitelio/química , Epitelio/embriología , Factores de Tiempo , Distribución TisularRESUMEN
OBJECTIVE: To evaluate if the peri-implantation endometrium shows age variations in lectin patterns, which suggest possible age variations in embryo-maternal recognition. STUDY DESIGN: Peri-implantation endometria of younger ( < 30 years of age: n = 13) and older ( > 40 years of age: n = 17) normally menstruating women was studied. Endometrial specimens were routinely fixed in buffered formaline and embedded in paraffin. Sections (5 microns) were studied using seven lectins: DBA (Dolicus biflorus, binding specificity alpha-D-GalNAc), PNA (Arachis hypogea, binding specificity D-Gal (beta 1 --> 3)-D-GalNAc), SBA (Glycine max binding specificity alpha/beta-D-GalNAc > D-Gal), WGA (Triticum vulgare binding specificity (alpha-D-GlcNAc)n and sialic acid), ConA (Canavalia ensiformis binding specificity alpha-D-Man > alpha-D-Glc), LTA (Lotus tetragonolobus binding specificity alpha-L-fucose) and UEA 1 (Ulex europaeus binding specificity alpha-L-fucose). RESULTS: No significant differences were found in the glycoconjugates sugar residue content and distribution between the endometria of women < 30 years of age and those of women > 40. CONCLUSIONS: Our results suggest that human endometrium does not age, at least while cyclic hormonal stimulation and menstruation are present.
Asunto(s)
Envejecimiento/metabolismo , Implantación del Embrión/fisiología , Endometrio/metabolismo , Lectinas/metabolismo , Adulto , Desarrollo Embrionario/fisiología , Femenino , Humanos , Persona de Mediana Edad , EmbarazoRESUMEN
A lectin histochemical study was performed to investigate the glycoconjugate saccharidic moieties of the lining and glandular epithelium of the chick embryo proventriculus from day 7 to hatching. The sugar residues D-galactose-(beta 1-->3)-N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, alpha/beta-N-acetyl-D-galactosamine and alpha-D-mannose were present at the lining epithelium of the proventriculus from the 7th day onwards; alpha-D-fucosyl residues were never detected. The same sugar residues were detected in the cells of the deep glands from the 7th day; alpha-L-fucose appeared later. The sugar residues appeared in the cells of the superficial glands at different times during the development of the proventriculus. In comparison to that of the deep glands, the secretion of the superficial glands was characterized, by the absence of N-acetylgalactosamine and alpha-D-mannose. From day 20 onwards, the composition of the mucus released by the two types of glands was identical from a qualitative point of view. The histological and histochemical results showed that the superficial glands are well defined morphological structures and not "an artefact of contraction", as claimed by other authors.
Asunto(s)
Glicoconjugados/metabolismo , Lectinas , Estómago de Aves/metabolismo , Animales , Secuencia de Carbohidratos , Embrión de Pollo , Células Epiteliales , Epitelio/embriología , Epitelio/metabolismo , Histocitoquímica , Datos de Secuencia Molecular , Estómago de Aves/citología , Estómago de Aves/embriologíaRESUMEN
The histochemical characteristics of the epithelial mucins, which are produced by the lining epithelium, the superficial glands and the compound glands of the proventriculus were investigated in the chick embryo from the 7th day of incubation to hatching. Results showed that the time of appearance of the carboxylated, sulphated and neutral mucosubstances differed at the three investigated sites. From the 13th to the 19th day of incubation, intense production of the various mucins was detected at the three sites. However, the modality of intracytoplasmatic storage and the production of mucins in both the cells of the lining epithelium and the superficial glands appeared to differ from those observed in the cells of the compound glands. At the 20th and 21st day, the mucins were primarily produced by the lining epithelium and the superficial glands, whereas production diminished in the compound glands. Furthermore, from the 7th to the 17th day of incubation, the cells of the principal ducts of the compound glands showed the same histochemical characteristics and modality of production as those of the glandular cells of the compound glands. On the other hand, from the 18th day to hatching, these cells assumed the same characteristics as those of the lining epithelium and of the superficial glands.
Asunto(s)
Mucinas/química , Proventrículo/embriología , Azul Alcián , Animales , Embrión de Pollo , Células Epiteliales , Epitelio/embriología , Epitelio/metabolismo , Histocitoquímica , Mucinas/biosíntesis , Proventrículo/citología , Proventrículo/metabolismo , Coloración y EtiquetadoRESUMEN
Using seven horseradish peroxidase-conjugated lectins we studied the distribution of glycoconjugate sugar residues in the lining and glandular epithelium of the gizzard in chick embryos from day 7 to 21 of incubation. At the outer layer of the lining epithelium all investigated sugar residues were present from day 7 onwards, except alpha-L-fucose, which was detected later. From day 9 some cells, which contained granules characterised by D-galactose-(beta 1--->3)-N-acetyl-D-galactosamine and N-acetyl-D-glucosamine, were observed in the inner part of the lining epithelium. Afterwards, although in different time periods, all investigated oligosaccharides were detected in intercellular spaces filled with mucous material. At first, the cells of the anlage of the tubular glands appeared to be characterised by D-glucosamine and alpha-D-mannose only. Afterwards, all the investigated sugar residues were detected. At hatching, the luminal glandular secretion showed all investigated sugar residues, except alpha-L-fucose, which was typical for the early formation of the tubular glands. Our data suggest that during the development of the gizzard three time periods of mucous release can be distinguished.
Asunto(s)
Metabolismo de los Hidratos de Carbono , Molleja de las Aves/metabolismo , Glicoconjugados/metabolismo , Lectinas/química , Animales , Secuencia de Carbohidratos , Carbohidratos/análisis , Carbohidratos/química , Embrión de Pollo , Células Epiteliales , Epitelio/metabolismo , Molleja de las Aves/embriología , Glicoconjugados/química , Histocitoquímica , Peroxidasa de Rábano Silvestre/química , Peroxidasa de Rábano Silvestre/metabolismo , Lectinas/metabolismo , Datos de Secuencia MolecularRESUMEN
The course of cranial nerves was studied by means of magnetic resonance (MR), which allowed a multiplane visualization of the investigated structures. The obtained results showed that MR was an excellent method for visualization of the optic, trigeminal, facial and acoustica nerves. The oculomotor and the abducent nerves were detectable only in some regions where the contrast with the surrounding structures was greater. The glossopharingeus, vegus and accessory nerves were identifiable only in the first tract of emergency from the encephalic trunk and they could no be distinguished separately. The trochlear nerve was seldom visible whereas the olfactory nerve failed to be revealed. Further technological progress will allow for additional advances as regards the acquisition of knowledge concerning these important nerves.