Partition of Anammox and Nitrifiers Through Bio-Carriers for Full-Scale Sidestream Partial Nitrification-Anammox Plant.

This study assessed the activity and community structure in different types of sludge to reveal the partition mechanism of anammox and nitrifiers in a full-scale partial nitrification-anammox plant. Batch experiments confirmed that suspended sludge had higher partial nitrification capacity, and biofilm sludge had higher anammox activity, 16.9 times higher than suspended sludge. qPCR analysis confirmed that the amoA gene was mainly present in suspended sludge, and the highest abundance of the Amx gene was observed in biofilm sludge, reaching 1.01 × 107 copies/ng DNA. High-throughput results revealed that Nitrosomonas was the main ammonia-oxidizing bacteria with high activity in suspended sludge, and Candidatus Brocadia had the highest abundance of 13.4% in biofilm sludge. This is the exploration of the microbial community of three different sludge types in the full-scale sidestream PN/A system for the first time, which can guide the construction and replication of full-scale PN/A plants.

Microbial diversity reveals the partial denitrification-anammox process serves as a new pathway in the first mainstream anammox plant.

A full-scale sewage treatment plant in Xi'an city is discovered as the first mainstream anaerobic ammonia oxidation (anammox) treatment process in China. Whether its biological mechanism is the nitritation-anammox or partial denitrification (PD)-anammox brought violent controversy between two groups. As a third party, here we uncovered the mystery of the moving-bed biofilm reactor (MBBR) as a PD-anammox process by analyzing the diversity and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) of microbes in anoxic pond. Anammox bacteria was found in the MBBR anoxic tank, which abundance is 8.9 times of that in the common anaerobic-anoxic-oxic process, confirming the existence of anammox process. The denitrifying bacteria (DNB) content in the anoxic tank is 5.9 times of the content of ammonia oxidizing bacteria (AOB), thus the DNB-anammox system is proved at the microbial composition level. The PICRUSt analysis found that ammonium nitrogen is mainly derived from the deamination of urea. The functional genes NAR and AMO of DNB and AOB are 910.84 and 5.80 rpms, respectively. The NAR gene content is 157.0 times of the AMO gene content and it is proved at the genetic level that the nitrite in the anoxic pool is mainly derived from denitrification. This study demonstrated the feasibility and advantages of the PD-anammox in the anammox process, which is different from the traditional nitritation-anammox demonstrated in Strass Wastewater Treatment Plant, Austria and Changi Water Reclamation Plant, Singapore and provided an alternative option for the mainstream application of anammox.

The distribution of dissimilatory nitrate reduction to ammonium bacteria in multistage constructed wetland of Jining, Shandong, China.

Dissimilatory nitrate reduction to ammonium (DNRA) is an important process of nitrate reduction in the environment. The distribution of DNRA bacteria and the relationships with environmental factors in multistage constructed wetland were investigated in this study. The quantitative real-time polymerase chain reaction analysis showed that the abundance of DNRA bacteria at all sites ranged from 2.10 × 1010 to 1.10 × 1011 copies/g of dry sediments. The Anaeromyxobacter (belong to Deltaproteobacteria) was the most abundant DNRA bacteria at all sites. The Geobater known as DNRA bacteria was also identified in this study. The abundances of DNRA bacteria, denitrifying bacteria, and anammox bacteria were conspicuously negatively correlated with Eh and positively correlated with the NO3--N removal efficency by statistical analysis.

Survey of dissimilatory nitrate reduction to ammonium microbial community at national wetland of Shanghai, China.

Dissimilatory nitrate reduction to ammonia (DNRA) process is an important nitrate reduction pathway in the environment. Numerous studies focused on the DNRA, especially in various natural habitats. However, little is known about the envrionmental parameters driving the DNRA process in anthropogenic ecosystem. Human activities put forward significant influence on nitrogen cycle and bacterial communities of sediment. This study aimed to assess the DNRA potential rates, nrfA gene abundance, DNRA bacterial community's diversity and influencing factors in a national wetland park near the Yangtze River estuary, Shanghai. The results of 15N isotope tracer experiments showed that DNRA potential rates from 0.13 to 0.44 µmol N/kg/h and contribution of nitrate reduction varied from 1.56% to 7.47%. The quantitative real-time PCR results showed that DNRA functional gene nrfA abundances ranged from 9.87E+10 to 1.98E+11 copies/g dry weight. The results of nrfA gene pyrosequencing analysis showed that Lacunisphaera (10.4-13.4%), Sorangium (7.1-10.7%), Aeromonas (4.2-6.8%), Corallococcus (1.8-6.9%), and Geobacter (3.3-6.6%) showed higher relative abundances in their genus levels. Combined with environmental parameters of sediments, redundancy analysis indicated that the nrfA functional gene was positively correlated with moisture content, the concentration of NO2--N and NO3-N; the DNRA rates was positively correlated with sediment organic carbon (SOC), C/NO3- ratio and salinity (ranked by explains %). This study is the first simultaneous determination of nitrate reduction pathways including denitrification, anammox and DNRA rates to assess the role of DNRA in a national wetland park and revealed the community abundance, diversity of DNRA bacteria and its relationship with environmental factors.

Autoclaved sludge as the ideal seed to culture anammox bacteria: Reactor performance and microbial community diversity.

Reducing activity of commensal bacteria in inocula may enhance anammox bacteria proliferation and realization of anammox process. Fast start-up of anammox process in an UASB reactor was successfully achieved by using autoclaved sludge (anaerobic granular sludge pretreated by autoclaving) and 0.3% active anammox sludge as inoculum. Continuous experiments indicated that R2 (autoclaved sludge addition) could shorten the start-up period from 72days to 63days. The first 50days anammox population specific growth rates (µ) of R1 (the control) and R2 were determined to be 0.014d-1 and 0.045d-1 using q-PCR assays. Analysis of coefficient of variations of nitrogen removal performance during days 96-225 indicated that R2 was more stable than R1. The Illumina MiSeq sequencing showed that autoclaving could decrease microbial diversity of sludge and enhance the abundance of anammox bacteria. Furthermore, PICRUSt community functions forecast and c-di-GMP measure illuminated the result of higher stability in R2.

Dissimilatory Nitrate Reduction to Ammonium in the Yellow River Estuary: Rates, Abundance, and Community Diversity.

Dissimilatory nitrate reduction to ammonium (DNRA) is an important nitrate reduction process in estuarine sediments. This study reports the first investigation of DNRA in the Yellow River Estuary located in Eastern Shandong, China. Saltwater intrusion could affect the physicochemical characteristics and change the microbial community structure of sediments. In this study, the activity, abundance and community diversity of DNRA bacteria were investigated during saltwater intrusion. The slurry incubation experiments combined with isotope-tracing techniques and qPCR results showed that DNRA rates and nrfA (the functional gene of DNRA bacteria) gene abundance varied over wide ranges across different sites. DNRA rates had a positive and significant correlation with sediment organic content and extractable NH4+, while DNRA rates were weakly correlated with nrfA gene abundance. In comparison, the activities and abundance of DNRA bacteria did not change with a trend along salinity gradient. Pyrosequencing analysis of nrfA gene indicated that delta-proteobacteria was the most abundant at all sites, while epsilon-proteobacteria was hardly found. This study reveals that variability in the activities and community structure of DNRA bacteria is largely driven by changes in environmental factors and provides new insights into the characteristics of DNRA communities in estuarine ecosystems.