RESUMEN
ß-arrestins (ßarr1 and ßarr2) are ubiquitous regulators of G protein-coupled receptor (GPCR) signaling. Available data suggest that ß-arrestins dock to different receptors in different ways. However, the structural characterization of GPCR-arrestin complexes is challenging and alternative approaches to study GPCR-arrestin complexes are needed. Here, starting from the finger loop as a major site for the interaction of arrestins with GPCRs, we genetically incorporate non-canonical amino acids for photo- and chemical crosslinking into ßarr1 and ßarr2 and explore binding topologies to GPCRs forming either stable or transient complexes with arrestins: the vasopressin receptor 2 (rhodopsin-like), the corticotropin-releasing factor receptor 1, and the parathyroid hormone receptor 1 (both secretin-like). We show that each receptor leaves a unique footprint on arrestins, whereas the two ß-arrestins yield quite similar crosslinking patterns. Furthermore, we show that the method allows defining the orientation of arrestin with respect to the GPCR. Finally, we provide direct evidence for the formation of arrestin oligomers in the cell.
Asunto(s)
Arrestina , Arrestinas , Arrestinas/genética , Arrestinas/metabolismo , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo , Transducción de Señal , beta-ArrestinasRESUMEN
Sensitive detection of small molecule fragments binding to defined sites of biomacromolecules is still a considerable challenge. Here we demonstrate that protein-binding fragments are able to induce enzymatic reactions on the protein surface via dynamic fragment ligation. Fragments binding to the S1 pocket of serine proteases containing a nitrogen, oxygen or sulphur nucleophile are found to activate electrophilic pre-substrates through a reversible, covalent ligation reaction. The dynamic ligation reaction positions the pre-substrate molecule at the active site of the protein thereby inducing its enzymatic cleavage. Catalytic activation of pre-substrates is confirmed by fluorescence spectroscopy and by high-performance liquid chromatography. The approach is investigated with 3 pre-substrates and 14 protein-binding fragments and the specific activation and the templating effect exerted by the enzyme is quantified for each protease-fragment-pre-substrate combination. The described approach enables the site-specific identification of protein-binding fragments, the functional characterization of enzymatic sites and the quantitative analysis of protein template-assisted ligation reactions.
Asunto(s)
Fragmentos de Péptidos/química , Serina Proteasas/química , Sitios de Unión , Biocatálisis , Dominio Catalítico , Humanos , Cinética , Fragmentos de Péptidos/metabolismo , Serina Proteasas/metabolismo , Especificidad por SustratoAsunto(s)
Alquenos/metabolismo , Aminas/metabolismo , Glucosa 1-Deshidrogenasa/metabolismo , Nitrocompuestos/metabolismo , Oxidorreductasas/metabolismo , Alquenos/química , Aminas/química , Biocatálisis , Glucosa 1-Deshidrogenasa/química , Metilación , Estructura Molecular , Nitrocompuestos/química , Oxidación-Reducción , Oxidorreductasas/química , EstereoisomerismoRESUMEN
Erysimum is a genus of the Brassicaceae family closely related to the genus Arabidopsis. Several Erysimum species accumulate 5ß-cardenolides. Progesterone 5ß-reductases (P5ßRs) first described in Digitalis species are thought to be involved in 5ß-cardenolide biosynthesis. P5ßRs belong to the dehydrogenase/reductase super-family of proteins. A full length cDNA clone encoding a P5ßR was isolated from Erysimum crepidifolium leaves by 5'/3' RACE-PCR (termed EcP5ßR). Subsequently, the P5ßR cDNAs of another nine Erysimum species were amplified by RT-PCR using 5' and 3' end primers deduced from the EcP5ßR cDNA. The EcP5ßR cDNA is 1170bp long and encodes for 389 amino acids. The EcP5ßR cDNA was ligated into the vector pQE 30 UA and the recombinant His-tagged protein (termed rEcP5ßR) was over-expressed in Escherichia coli and purified by Ni-chelate affinity chromatography. Kinetic constants were determined for progesterone, 2-cyclohexen-1-one, isophorone, and NADPH. The by far highest specificity constant (k(cat)K(M)⻹) was estimated for 2-cyclohexen-1-one indicating that this monocyclic enone may be more related to the natural substrate of the enzyme than progesterone. The atomic structure of rEcP5ßR was modelled using the crystal structure of P5ßR from Digitalis lanata 2V6G as the template. All sequence motifs specific for SDRs as well as the NFYYxxED motif typical for P5ßR-like enzymes were present and the protein sequence fitted into the template smoothly.
Asunto(s)
Cardenólidos/metabolismo , Ciclohexanonas/metabolismo , Erysimum/enzimología , Oxidorreductasas/metabolismo , Progesterona/metabolismo , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Clonación Molecular , Secuencia de Consenso , ADN Complementario/genética , Erysimum/química , Erysimum/genética , Erysimum/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Cinética , Datos de Secuencia Molecular , Oxidorreductasas/química , Oxidorreductasas/genética , Oxidorreductasas/aislamiento & purificación , Filogenia , Hojas de la Planta/enzimología , ARN de Planta/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Especificidad por SustratoRESUMEN
Most cardenolides used in the therapy of cardiac insufficiency are 5 beta-configured and thus the stereo-specific reduction of the Delta(4,5)-double bond of a steroid precursor is a crucial step in their biosynthesis. This step is thought to be catalysed by progesterone 5 beta-reductases. We report here on the isolation of 11 progesterone 5 beta-reductase (P5 beta R) orthologues from 5 beta-cardenolide-free and 5 beta-cardenolide-producing plant species belonging to five different angiosperm orders (Brassicales, Gentianales, Lamiales, Malvales and Solanales). Amino acid sequences of the P5 beta R described here were highly conserved. They all contain certain motifs qualifying them as members of a class of stereo-selective enone reductases capable of reducing activated C=C double bonds by a 1,4-addition mechanism. Protein modeling revealed seven conserved amino acids in the substrate-binding/catalytic site of these enzymes which are all supposed to exhibit low substrate specificity. Eight P5 beta R genes isolated were expressed in Escherichia coli. Recombinant enzymes reduced progesterone stereo-specifically to 5 beta-pregane-3,20-dione. The progesterone 5 beta-reductases from Digitalis canariensis and Arabidopsis thaliana reduced activated C=C double bonds of molecules much smaller than progesterone. The specific role of progesterone 5 beta-reductases of P5 beta Rs in cardenolide metabolism is challenged because this class of enone reductases is widespread in higher plants, and they accept a wide range of enone substrates.