RESUMEN
A fatality following ingestion of sulpiride is presented. The drug was identified and quantitated in postmortem blood by gas chromatography-mass spectrometry and high-performance liquid chromatography with diode-array detection. The concentration was 38 microg/mL, which was in excess of 34 times the therapeutic concentration of sulpiride. For other associated drugs, their concentrations were in their therapeutic ranges.
Asunto(s)
Antipsicóticos/envenenamiento , Sulpirida/envenenamiento , Adulto , Antipsicóticos/análisis , Sobredosis de Droga , Resultado Fatal , Femenino , Cromatografía de Gases y Espectrometría de Masas , Humanos , Sulpirida/análisisRESUMEN
A case of lethal overdose by heroin ingestion is presented. The concentrations of drugs were measured several hours after death. Heroin, 06-monoacetylmorphine, and morphine were identified and quantitated in blood, urine, and gastrointestinal contents by gas chromatography-mass spectrometry and high-performance liquid chromatography. Concentrations of heroin, 06-monoacetylmorphine, and morphine were 109, 168, and 1140 ng/mL, respectively, in blood and 17, 12, and 425 ng/g, respectively, in gastrointestinal content. In urine, however, only morphine was detected at 3650 ng/mL.
Asunto(s)
Heroína/envenenamiento , Derivados de la Morfina/análisis , Morfina/análisis , Narcóticos/envenenamiento , Trastornos Relacionados con Sustancias , Administración Oral , Adulto , Calibración , Cromatografía Líquida de Alta Presión , Sobredosis de Droga , Resultado Fatal , Cromatografía de Gases y Espectrometría de Masas , Contenido Digestivo/química , Heroína/análisis , Heroína/sangre , Humanos , Masculino , Morfina/sangre , Morfina/orina , Derivados de la Morfina/sangre , Narcóticos/efectos adversos , Narcóticos/sangre , Estándares de ReferenciaRESUMEN
6-Monoacetylmorphine and morphine were determined simultaneously in plasma, whole blood and urine, after solid-phase extraction, by high-performance liquid chromatography using amperometric detection at 600 mV oxidation potential. The recoveries ranged from 92 to 99%. The reproducibility study indicated that the coefficients of variation were less than 11% for morphine and 12.4% for 6-monoacetylmorphine. The determination limits were 1 ng/ml for morphine and 4 ng/ml for 6-monoacetylmorphine. The method had a good selectivity towards opiate and nonopiate analgesics and other drugs. The stability of the analytes in methanol (standard solutions), in samples (plasma, whole blood and urine) at -20 degrees C and at 20 degrees C, and in samples after enzymatic hydrolysis at 37 degrees C, was also studied. For sample containing 6-monoacetylmorphine, inadequate storage or hydrolysis could lead to overestimation of morphine or its conjugates. The technique described can be applied for the study of the pharmacokinetics of heroin; it is also available for forensic toxicology to distinguish heroin use from medical prescription of morphine and other opiate drugs.