RESUMEN
The aim of this study was to assess the diagnostic value of Peroxidase Linked Assay (PLA) for detecting the bovine leukemia virus (BLV) infection. Two negative and two positive reference sera were applied to 204 serum samples from dairy cows from 3 different farms (I, II and III). The best results of serological testing were obtained in the herd infected with BLV for several years (I). Amongst 10 serum samples taken at random, all were positive in AGID, ELISA and PLA. Serological tests of samples from group II revealed: 34 (73.9%) positive results in AGID and 39 (84.7%) either in ELISA or PLA. In group III positive results in AGID occured in 81.8% of cases, and 91.9% and 92.9% in ELISA and PLA respectively. The mean OD value for positive samples in groups II and III ranged between 0.943 and 0.215. Immunoperoxidase PLA is a specific and sensitive test for detection of BLV infections.
Asunto(s)
Anticuerpos Antivirales/sangre , Leucosis Bovina Enzoótica/diagnóstico , Ensayo de Inmunoadsorción Enzimática/veterinaria , Inmunodifusión/veterinaria , Virus de la Leucemia Bovina/inmunología , Animales , Bovinos , Industria Lechera , Leucosis Bovina Enzoótica/sangre , Femenino , Virus de la Leucemia Bovina/aislamiento & purificación , Valor Predictivo de las Pruebas , Sensibilidad y EspecificidadRESUMEN
This study demonstrates the usefulness of PLA and nested-PCR methods for the detection of cattle infection with enzootic bovine leukemia virus. Serological control with PLA was by 1.7% more sensitive than reference ELISA examination. Both the DNA of env-BLV gene in NCR, V-NCR, FLK and in recombinated AG clone cells, as well as in the whole blood and in blood isolated leukocytes of dairy cattle, were detected with the nested-PCR.
Asunto(s)
Leucosis Bovina Enzoótica/diagnóstico , Virus de la Leucemia Bovina/inmunología , Reacción en Cadena de la Polimerasa/veterinaria , Animales , Anticuerpos Antivirales/sangre , Bovinos , ADN Viral/sangre , Industria Lechera , Leucosis Bovina Enzoótica/sangre , Leucosis Bovina Enzoótica/virología , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Inmunodifusión/veterinaria , Virus de la Leucemia Bovina/aislamiento & purificación , Reacción en Cadena de la Polimerasa/normas , Valor Predictivo de las Pruebas , Sensibilidad y EspecificidadRESUMEN
The bovine leukemia virus (BLV) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into a vehicle expression vector under the human cytomegalovirus (CMV) intermediate early promoter. The intramuscular injection of this plasmid vector generated a cellular immune response. Seven out of ten cows vaccinated with the DNA construct resisted a drastic challenge (500 BLV-infected lymphocytes as an infectious dose).
Asunto(s)
Leucosis Bovina Enzoótica/prevención & control , Virus de la Leucemia Bovina/genética , Virus de la Leucemia Bovina/inmunología , Vacunas de ADN/farmacología , Vacunas Virales/farmacología , Animales , Anticuerpos Antivirales/biosíntesis , Secuencia de Bases , Bovinos , Cartilla de ADN/genética , Leucosis Bovina Enzoótica/inmunología , Genes env , Humanos , Inmunidad Celular , Plásmidos/genética , Vacunas de ADN/administración & dosificación , Vacunas Virales/administración & dosificaciónRESUMEN
Nine groups of sheep have been experimentally infected with different doses of BLV and by various routes. An immunological response of animals, as well as clinical symptoms of leukosis in the experimental sheep were investigated within several years or months. The animals inoculated with the virus showed appearance of BLV-antibodies in 94 per cent of cases, lymphocytosis in 47 per cent and clinical symptoms of the disease in 33 per cent. In conclusion, sheep have been demonstrated to be animals suitable for experimental study of leukosis.