RESUMEN
AIMS: Immunohistochemistry is a highly valuable and widely used tool in the subtyping of lung carcinomas. The aim of this study was to identify markers for the differential diagnosis of non-small-cell carcinomas. METHODS AND RESULTS: We report on the immunohistochemical localization of plakophilin-1 (PKP1), keratin-15 (KRT15) and desmoglein-3 (DSG3) intercellular adhesion proteins in samples from 75 primary non-small-cell lung cancers in non-treated patients. The staining pattern of these proteins differed between squamous cell carcinomas and adenocarcinomas, with no membrane staining in the latter. Membrane staining for all three proteins was characteristic of squamous cell carcinomas. We observed a relationship between the presence/absence of these proteins in the membranes of squamous cell carcinomas and the differentiation grade, with more intense staining in better differentiated areas. CONCLUSIONS: Staining for these proteins marked intercellular junctions that are characteristic of stratified squamous epithelium and of neoplasias with this type of differentiation, and can be useful in the diagnosis of patients with squamous cell carcinoma of the lung. The high specificity of membrane staining for PKP1 and DSG3 and high sensitivity of cytoplasmic and membrane staining for KRT15 for the diagnosis of squamous cell carcinoma may be useful for the differential diagnosis of non-small-cell carcinomas.
Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/patología , Desmogleína 3/metabolismo , Desmosomas/patología , Queratina-15/metabolismo , Neoplasias Pulmonares/patología , Placofilinas/metabolismo , Biomarcadores de Tumor/metabolismo , Carcinoma de Pulmón de Células no Pequeñas/diagnóstico , Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patología , Diferenciación Celular , Desmosomas/metabolismo , Diagnóstico Diferencial , Humanos , Inmunohistoquímica , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/metabolismoRESUMEN
La disgenesia tubular renal es una enfermedad adquirida durante el desarrollo fetal o heredada, con un patrón autosómico recesivo. Histológicamente es una severa anomalía del desarrollo de los túbulos renales. Clínicamente, se caracteriza por anuria fetal persistente y muerte perinatal, probablemente debido a hipoplasia pulmonar y secuencia Potter. Presentamos el caso de un recién nacido de sexo femenino que murió horas después del nacimiento por insuficiencia renal y respiratoria. La necropsia informó de disgenesia tubular renal (AU)
Renal tubular dysgenesis is acquired during fetal development or through autosomal recessive inheritance. Histologically, this entity is a severe disorder affecting renal tubular development. Clinically, renal tubular dysgenesis is characterized by persistent fetal anuria and perinatal demise, probably due to pulmonary hypoplasia and Potter sequence. We report the case of a female neonate who died a few hours after birth from renal and respiratory failure. Post-mortem examination identified renal tubular dysgenesis (AU)
