RESUMEN
BACKGROUND: Mexico is considered the diversification center for chili species, but these crops are susceptible to infection by pathogens such as Colletotrichum spp., which causes anthracnose disease and postharvest decay in general. Studies have been carried out with isolated strains of Colletotrichum in Capsicum plants; however, under growing conditions, microorganisms generally interact with others, resulting in an increase or decrease of their ability to infect the roots of C. chinense seedlings and thus, cause disease. RESULTS: Morphological changes were evident 24 h after inoculation (hai) with the microbial consortium, which consisted primarily of C. ignotum. High levels of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA) were found around 6 hai. These metabolic changes could be correlated with high transcription levels of diacylglycerol-kinase (CchDGK1 and CchDG31) at 3, 6 and 12 hai and also to pathogen gene markers, such as CchPR1 and CchPR5. CONCLUSIONS: Our data constitute the first evidence for the phospholipids signalling events, specifically DGPP and PA participation in the phospholipase C/DGK (PI-PLC/DGK) pathway, in the response of Capsicum to the consortium, offering new insights on chilis' defense responses to damping-off diseases.
Asunto(s)
Capsicum/inmunología , Colletotrichum/fisiología , Consorcios Microbianos/fisiología , Fosfolípidos/metabolismo , Enfermedades de las Plantas/inmunología , Inmunidad de la Planta , Transducción de Señal , Capsicum/genética , Capsicum/microbiología , Colletotrichum/aislamiento & purificación , Diacilglicerol Quinasa , Difosfatos/metabolismo , Glicerol/análogos & derivados , Glicerol/metabolismo , Interacciones Huésped-Patógeno , Ácidos Fosfatidicos/metabolismo , Filogenia , Enfermedades de las Plantas/microbiología , Raíces de Plantas/genética , Raíces de Plantas/inmunología , Raíces de Plantas/microbiología , Plantones/genética , Plantones/inmunología , Plantones/microbiología , Fosfolipasas de Tipo C/metabolismoRESUMEN
The protective effect of salicylic acid (SA) on aluminum (Al) toxicity was studied in suspension cells of Coffea arabica L. The results showed that SA does not produce any effect on cell growth and that the growth inhibition produced by aluminum is restored during simultaneous treatment of the cells with Al and SA. In addition, the cells exposed to both compounds, Al and SA, showed evident morphological signals of recovery from the toxic state produced in the presence of Al. The cells treated with SA showed a lower accumulation of Al, which was linked to restoration from Al toxicity because the concentration of Al(3+) outside the cells, measured as the Al(3+)-morin complex, was not modified by the presence of SA. Additionally, the inhibition of phospholipase C by Al treatment was restored during the exposure of the cells to SA and Al. The involvement of protein phosphorylation in the protective effect of SA on Al-toxicity was suggested because staurosporine, a protein kinase inhibitor, reverted the stimulatory effect of the combination of Al and SA on protein kinase activity. These results suggest that SA attenuates aluminum toxicity by affecting a signaling pathway linked to protein phosphorylation.
Asunto(s)
Aluminio/farmacología , Coffea/metabolismo , Proteínas de Plantas/metabolismo , Ácido Salicílico/farmacología , Aluminio/metabolismo , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Coffea/citología , Coffea/ultraestructura , Relación Dosis-Respuesta a Droga , Antagonismo de Drogas , Inhibidores Enzimáticos/farmacología , Microscopía Electrónica de Rastreo , Fosforilación/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Estaurosporina/farmacología , Factores de Tiempo , Fosfolipasas de Tipo C/metabolismoRESUMEN
Signal transduction via phospholipids is mediated by phospholipases such as phospholipase C (PLC) and D (PLD), which catalyze hydrolysis of plasma membrane structural phospholipids. Phospholipid signaling is also involved in plant responses to phytohormones such as salicylic acid (SA). The relationships between phospholipid signaling, SA, and secondary metabolism are not fully understood. Using a Capsicum chinense cell suspension as a model, we evaluated whether phospholipid signaling modulates SA-induced vanillin production through the activation of phenylalanine ammonia lyase (PAL), a key enzyme in the biosynthetic pathway. Salicylic acid was found to elicit PAL activity and consequently vanillin production, which was diminished or reversed upon exposure to the phosphoinositide-phospholipase C (PI-PLC) signaling inhibitors neomycin and U73122. Exposure to the phosphatidic acid inhibitor 1-butanol altered PLD activity and prevented SA-induced vanillin production. Our results suggest that PLC and PLD-generated secondary messengers may be modulating SA-induced vanillin production through the activation of key biosynthetic pathway enzymes.