Autologous Single Semitendinosus Anatomical Posterolateral Corner Reconstruction With Adjustable-Loop Cortical Suspension Devices.

We present our surgical technique for the reconstruction of the posterolateral corner of the knee. It is a tibia- and fibular-based reconstruction technique. Most of these procedures require the use of 2 tendons (autograft or allograft). In our technique, a single semitendinosus tendon is required, making the procedure more suitable if the surgeon prefers the use of autograft or when there is no access to a tissue bank. This is even more important in the setting of multiligament knee injuries. The most defining feature of this modification is the possibility of achieving the desired graft tension in a progressive and independent way, due to the use of 3 adjustable-loop cortical suspension devices.

Effect of long-term aerobic, anaerobic and aerobic-anaerobic physical training in seric toxic minerals concentrations.

BACKGROUND: Many substances poured out from industries can be toxic to humans and can impair physical performance. Besides, physical training may modify the body concentrations of these substances as a result of physiological adaptations. OBJECTIVES: The aim of the study was to determine if different modalities of exercise might affect serum concentrations of toxic trace elements in sportsmen. METHODS: 80 Spanish national sportsmen were recruited before the start of their training period. All the athletes had been training regularly for the previous two years with a rigorous training target at high-level competition. 31 sedentary participants from the same geographic area formed the control group. Serum arsenic, beryllium, cadmium, cesium and lead samples were analyzed with an ICP-MS. RESULTS: Serum concentrations were higher among the sportsmen group than among the control group, being highly significant in cases of Be from 0.043±0.019 to 0.074±0.029µg/L, Cs from 0.693±0.305 to 1.358±0.569µg/L and Pb from 0.162±0.171 to 2.375±1.699µg/L; and significant in the case of Cd from 0.046±0.027 to 0.067±0.059µg/L. However, if they were separated according to different sport modalities, it was found that, although they had higher concentrations than controls, there were elements that changed their concentrations in relation to the metabolic type of activity performed. CONCLUSIONS: In some cases physical exercise induces favorable adaptations to avoid environmental pollution damage. Endurance training (65-75% VO2max) can be considered the most effective exercise to prevent toxicity effects. However, integral-matrixes analysis are required in further research to overcome some controversial behaviors of some elements.

The effect of CYP2J2, CYP3A4, CYP3A5 and the MDR1 polymorphisms and gender on the urinary excretion of the metabolites of the H-receptor antihistamine ebastine: a pilot study.

AIMS: To determine the effect of gender and the genetic polymorphisms of CYP2J2, CYP3A4, CYP3A5 and MDR1 on the urinary excretion of the H(1) antihistamine ebastine in healthy subjects. METHODS: Eighty-nine Caucasians were studied. The presence of polymorphisms in genes known to be involved in ebastine metabolism and transport (CYP2J2*2,*3,*4,*6,*7, CYP3A4*1B, CYP3A5*3, *6 and MDR1(ABCB1)(C3435T)) was assessed by means of PCR-restriction fragment length polymorphism and sequencing methods. Genotype was correlated with the urinary excretion of the main ebastine metabolites (desalkylebastine and carebastine) under basal conditions and after administration of grapefruit juice. RESULTS: Women excreted statistically greater amounts of desalkylebastine in urine (mean +/- SD (95% confidence intervals, 95% CI), 23.0 +/- 19.5 (18.1, 27.9) micromol) than men (12.4 +/- 11.0 (7.9, 16.9)), (mean difference: 10.6 (2.4, 18.7), P < 0.005). The CYP2J2, CYP3A4 and CYP3A5 analysed polymorphisms did not greatly affect ebastine metabolite excretion. The MDR1(C3435T) polymorphism was found to affect both the urinary excretion of the active metabolite carebastine (32.3 +/- 18.3 (23.1, 41.4), 22.8 +/- 14.7 (18.6, 27.0) and 21.5 +/- 15.3 (14.7, 28.3) for CC, CT and TT carriers, respectively; P < 0.05) and the grapefruit juice-induced inhibition of its transport/formation (mean fold-decrease +/- SD (95% CI), 1.5 +/- 0.8 (1.0, 2.0), 1.1 +/- 0.9 (0.7, 1.4) and 0.9 +/- 0.4 (0.6, 1.2) for CC, CT and TT carriers, respectively; P = 0.01). CONCLUSIONS: Gender and the presence of the MDR1(C3435T) polymorphism both influence the excretion of ebastine metabolites in urine.