Uso de técnica adaptada para cavotomia em cão com feocromocitoma associado a trombo neoplásico / Adapted cavotomy technique for pheochromocytoma associated tumor thrombi in a dog

Background: In dogs pheochromocytoma usually compress and invade into the adjacent vessels and structures, whichmay lead to the occurrence of caudal vena caval tumor thrombi. The surgical approach in these cases requires adrenalectomy with caval thrombectomy. Conventionally, tourniquets and partial occlusion clamp are used to reduce intraoperativeblood loss, but in dogs with a large thrombus associated to a great local tumor invasion, the traditional technique may behindered. The aim of this study was to report the use of a purse-string suture during venotomy of the caudal vena cava forthrombectomy, as an alternative to tourniquet and partial occlusion clamp.Case: An 11-year-old male Daschound breed dog, was referred for evaluation because of the appearance of small blackenednodules in the ventral abdomen. During a search for metastatic lesions with abdominal ultrasound, the right adrenal wasidentified with significant volume increase, and presence of tumor-associate circular formation that appeared to invade thecaudal vena cava, suggestive of neoplastic invasion or thrombus. Abdominal computed tomography showed caudal venacava in its hepatic portion presenting considerable increase in diameter, with caudal dilation and presence of hypoattenuatingmaterial in its lumen. Right adrenal gland with rounded appearance and regular borders, heterogeneous parenchyma andjuxtaposed caudal vena cava suggesting invasion of this, confirming the sonographic findings. During exploratory celiotomy,adherence of the adrenal mass to the right renal vessels and invasion of the caudal vena cava were observed, leading to theneed of nephrectomy and venotomy for thrombectomy. To perform the venotomy, a Rumel tourniquet was placed looselyaround the vena cava only caudal to the invasion point of the tumor thrombus, cranially, tourniquet application was notpossible due to the presence of a large thrombus extending into the intrahepatic cava...(AU)

Uso de técnica adaptada para cavotomia em cão com feocromocitoma associado a trombo neoplásico / Adapted cavotomy technique for pheochromocytoma associated tumor thrombi in a dog

Background: In dogs pheochromocytoma usually compress and invade into the adjacent vessels and structures, whichmay lead to the occurrence of caudal vena caval tumor thrombi. The surgical approach in these cases requires adrenalectomy with caval thrombectomy. Conventionally, tourniquets and partial occlusion clamp are used to reduce intraoperativeblood loss, but in dogs with a large thrombus associated to a great local tumor invasion, the traditional technique may behindered. The aim of this study was to report the use of a purse-string suture during venotomy of the caudal vena cava forthrombectomy, as an alternative to tourniquet and partial occlusion clamp.Case: An 11-year-old male Daschound breed dog, was referred for evaluation because of the appearance of small blackenednodules in the ventral abdomen. During a search for metastatic lesions with abdominal ultrasound, the right adrenal wasidentified with significant volume increase, and presence of tumor-associate circular formation that appeared to invade thecaudal vena cava, suggestive of neoplastic invasion or thrombus. Abdominal computed tomography showed caudal venacava in its hepatic portion presenting considerable increase in diameter, with caudal dilation and presence of hypoattenuatingmaterial in its lumen. Right adrenal gland with rounded appearance and regular borders, heterogeneous parenchyma andjuxtaposed caudal vena cava suggesting invasion of this, confirming the sonographic findings. During exploratory celiotomy,adherence of the adrenal mass to the right renal vessels and invasion of the caudal vena cava were observed, leading to theneed of nephrectomy and venotomy for thrombectomy. To perform the venotomy, a Rumel tourniquet was placed looselyaround the vena cava only caudal to the invasion point of the tumor thrombus, cranially, tourniquet application was notpossible due to the presence of a large thrombus extending into the intrahepatic cava...

Prenatal development of the agouti (Dasyprocta prymnolopha Wagler, 1831): External features and growth curves.

The gestation period in agoutis can range from 104 to 120 days. Knowledge regarding the morphological characteristics of embryos and fetuses is important as a base for studies in reproduction biotechnology, such as in vitro fertilization, embryo transfer and helps in determining congenital anomalies during the development phase. Thus, given the importance and lack of information about agouti embryology, the objective of this study was to characterize the external morphology and define the biometry of embryos and fetuses, at different days of development. Nine females were submitted to daily colpocytology to identify the estrus, confirm mating and identify day zero of the gestation. When the mating was confirmed they were weighed, underwent abdominal ultrasonography and surgery was conducted on the females at the gestational ages of 25, 30, 35, 40, 45, 50, 75 and 100 days. Sixteen embryos/fetuses were weighed and measured. Agouti embryos at 25 days after mating are "C" shaped, with primitive structures, 0.4±0.01cm crown-rump and weighed 0.06±0.01g; at 30 days after mating the crown-rump was 0.95±0.07cm and weighed 0.28±0.00g; at 35 days after mating the crown-rump was 155±0.07cm and weighed 0.38±0.01g; at 40 days after mating the crown-rump was 2.25±0.21cm and weighed 1.25±0.07g; at 45 days after mating the crown-rump was 3.45±0.35cm and weighed 2.75±0.64g; at 50 days after mating the crown-rump was 5.0±0.3cm and weighed 7.01±2.6g; at 75 days after mating, the skin was dark, the crown-rump was 10.0±0.14cm and weighed 55.2±0.07g. At 100 days after mating, the crown-rump was 13.8±0.49cm and fetuses weighed 136.7±9.40g. Based on the morphological data assessed the embryo and fetus age could be assessed and the size and average weight of agouti embryos was established.

Kidney injury and cell therapy: Preclinical study.

The aim of this study is to show histological and immunofluorescence analysis of renal parenchyma of agoutis affected by gentamicin-induced renal disease after the infusion of bone marrow mononuclear cells (BMMC) stained with Hoechst®. Nine agouti's males were divided into three groups: Test group (TG): renal disease by gentamicin induced (n = 3), cell therapy group (CTG): renal disease by gentamicin induced and BMMC infusion (n = 3), and control group (CG): nonrenal disease and BMMC infusion (n = 3). TG and CTG were submitted to the protocol of renal disease induction using weekly application of gentamicin sulfate for 4 months. CG and CTG received a 1 × 108 BMMC stained with Hoechst and were euthanized for kidney examination 21 days after BMMC injection and samples were collected for histology and immunofluorescence analysis. Histological analysis demonstrated typical interstitial lesions in kidney similarly to human disease, as tubular necrosis, glomerular destruction, atrophy tubular, fibrotic areas, and collagen deposition. We conclude that histological analysis suggest a positive application of agouti's as a model for a gentamicin inducing of kidney disease, beyond the immunofluorescence analysis suggest a significant migration of BMMC to sites of renal injury in CTG.

Padrão de distribuição de células mononucleares de medula óssea em tecido cardíaco sadio por diferentes vias de infusão / Interaction between infusion route and bone marrow mononuclear cell distribution patterns in normal cardiac tissue

Introdução: A medicina regenerativa tem ganho grande importância nos últimos anos em decorrência da possibilidade de certas células se diferenciarem em linhagens celulares distintas e, assim, reconstruírem o tecido lesado. As células-tronco têm despontado como forma alternativa de tratamento para doenças pela sua capacidade de diferenciação nos mais de 100 tipos de tecido. A medula óssea contém células-tronco adultas, hematopoéticas e mesenquimais, que auxiliam na limitação do remodelamento cardíaco. Método: Foram utilizados 9 cães com peso entre 25 kg e 30 kg, divididos em três grupos: intracoronária, intramiocárdica-transendocárdica e retrógrada venosa. Células mononucleares da medula óssea foram coletadas por densidade Ficoll, marcadas com fluorocromo Hoechst e infundidas nas diferentes vias citadas anteriormente...

Background: Regenerative medicine has become increasingly important in recent years due to the possibility of certain cells to differentiate into different cell lines and thus recover the damaged tissue. The stem cell has emerged as an alternative treatment for diseases as a result of their ability to differentiate in more than 100 types of tissue. Bone marrow contains adult stem cells, hematopoietic and mesenchymal cells, which limit heart remodeling. Methods: Nine dogs weighing between 25 and 30 kg were divided into three groups: intracoronary group, intramyocardial-transendocardial group and retrograde venous group. Mononuclear cells were collected from bone marrow by Ficoll density, stained with Hoechst fluorocrom and infused through the different routes mentioned above...