Micorrhizal biotechnology as an alternative to potentialize the strawberry quality / Biotecnologia micorrízica como alternativa para potencializar a qualidade do morangueiro

The need to develop and use biotechnology tools to improve management and nutritional techniques in strawberry cultivation are increasing. Based on this, the objective of this study was to test if the mycorrhizal inoculation changes the agronomic and qualitative performance of strawberry fruits. The strawberry daughters plants used were of the cultivar Camarosa. The study was carried out in an agricultural greenhouse, Horticulture Sector of the Faculty of Agronomy and Veterinary Medicine (FAMV) of UPF, in the city of Passo Fundo, Rio Grande do Sul (RS), Brazil. The experiment was developed and maintained from June to December 2015. The treatments consisted of five inoculum: T1 = no inoculum (control); T2 = Acaulospora morrowiae; T3 = Rhizophagus clarus; T4 = mycorrhizal community; T5 = Claroideoglomus etunicatum. The experimental design was a randomized block with four replicates. Each plot consisted of a bag containing 6 plants spaced 0.15 mx 0.15 m. In the pits of the plants, it was applied, with the aid of syringe and water, about 70 infective propagules of the tested inocula. During the conduction of the experiment the temperature and photosynthetically active radiation (PAR) were monitored in the culture environment. It was evaluated the agronomic and qualitative performance of the fruits and the percentage of mycorrhizal colonization of the plants roots. The data were submitted to analysis of variance and the differences between means were compared by the Tukey test at 5% of probability error. The cultivar Camarosa produced more fruits in October. In September, the month before the one with the best agronomic performance, the mean temperature was 17.7 ºC. The mean PAR recorded in the growing environment was low in relation to crop requirements, throughout the growing period. Fruits produced by plants inoculated with A. morrowiae presented higher values of TSS/TTA ratio in September and October. The percentage of mycorrhizal colonization varied from 26.2% for A. morrowiae to 46.2% for R. clarus. The inoculation of arbuscular mycorrhizal fungi does not alter the production of strawberry fruits. However, when the plants are inoculated with A. morrowiae there is an improvement in the fruit flavor.

A necessidade de desenvolver e usar ferramentas biotecnológicas para melhorar o manejo e as técnicas nutricionais no cultivo do morangueiro está aumentando. Com base nisso, o objetivo desse estudo foi testar se a inoculação micorrízica altera o desempenho agronômico e qualitativo de frutos de morangueiro. As mudas de morangueiro usadas foram da cultivar Camarosa. O estudo foi realizado em estufa agrícola, no Setor de Horticultura da Faculdade de Agronomia e Medicina Veterinária (FAMV) da UPF, na cidade de Passo Fundo, Rio Grande do Sul (RS), Brasil. O experimento foi desenvolvido e mantido de junho a dezembro de 2015. Os tratamentos consistiram em cinco inóculos: T1 = sem inóculo (controle); T2 = Acaulospora morrowiae; T3 = Rhizophagus clarus; T4 = comunidade micorrízica; T5 = Claroideoglomus etunicatum. O delineamento experimental foi em blocos casualizados, com quatro repetições. Cada parcela consistiu de um saco contendo 6 plantas espaçadas de 0.15 mx 0.15 m. Na cova de plantio das plantas, foi aplicado, com auxílio de seringa e água, cerca de 70 propágulos infectivos dos inóculos testados. Durante a condução do experimento, a temperatura e a radiação fotossinteticamente ativa (PAR) foram monitoradas no ambiente de cultivo. Foi avaliado o desempenho agronômico e qualitativo dos frutos e a porcentagem de colonização micorrízica das raízes das plantas. Os dados foram submetidos à análise de variância e as diferenças entre médias foram comparadas pelo teste de Tukey, a 5% de erro de probabilidade de erro. A cultivar Camarosa produziu mais frutos em outubro. Em setembro, no mês anterior ao de melhor desempenho agronômico, a temperatura média foi de 17.7ºC. A PAR média registrada no ambiente de cultivo foi baixa em relação às necessidades da cultura durante todo o período de cultivo. Frutos produzidos por plantas inoculadas com A. morrowiae apresentaram maiores valores da relação SST/ATT em setembro e outubro. A porcentagem de colonização micorrízica variou de 26.2% para A. morrowiae a 46.2% para R. clarus. A inoculação de fungos micorrízicos arbusculares não altera a produção de frutos de morangueiro. No entanto, quando as plantas são inoculadas com A. morrowiae, há uma melhora no sabor dos frutos.

Berries grown in Brazil: anthocyanin profiles and biological properties.

BACKGROUND: Phytochemical profiles of two Brazilian native fruits - pitanga (red and purple) and araçá (yellow and red) - as well as strawberry cultivars Albion, Aromas and Camarosa, blackberry cultivar Tupy and blueberry cultivar Bluegen cultivated in Brazil were characterized for total phenolic content and total anthocyanin content by liquid chromatography coupled to a photodiode array and a quadrupole time-of-flight mass spectrometer. Radical scavenging, antiherpes and cytotoxic activities of these berry extracts were also evaluated. RESULTS: Blueberry presented the highest total anthocyanin content (1202 mg cyanidin-O-glucoside equivalents kg-1 fresh fruit), while strawberry cultivar Aromas presented the highest total phenolic content (13 550 mg gallic acid equivalents kg-1 fresh fruit). Liquid chromatographic-mass spectrometric analysis resulted in the identification of 21 anthocyanins. To the best of our knowledge this is the first report of cyanidin-O-glucoside in yellow and red Araçá fruit and the first time eight anthocyanins have been reported in pitanga fruits. DPPH and ABTS assays showed that blueberry cultivar Bluegen, blackberry cultivar Tupy and pitanga (red and purple) showed the most promising antiradical activities, respectively. No relevant cytotoxicity against three cancer cell lines or antiherpes activity was detected under the experimental conditions tested. CONCLUSION: Total anthocyanin content of all fruits had a strong positive correlation with their free radical scavenging activity, suggesting anthocyanins contribute to the antioxidant potential of these fruits. © 2018 Society of Chemical Industry.

Molecular mechanism of action of Pelargonidin-3-O-glucoside, the main anthocyanin responsible for the anti-inflammatory effect of strawberry fruits.

Fragaria x ananassa Duch., popularly called strawberry, is known for its worldwide consumption and important biological activities, and these effects are related to its high concentration of anthocyanins. Pelargonidin-3-O-glucoside (P3G) is a major anthocyanin found in strawberry, and was evaluated for its anti-inflammatory action in experimental models. The effect of strawberry extract and P3G, on leukocyte migration, exudation levels and many inflammatory mediators, was therefore evaluated in an in vivo model. An in vitro study was also carried out to characterize the effect of P3G on mitogen-activated protein kinases, and on nuclear transcript factors NF-κB and AP-1. The results revealed that the strawberry and P3G have important anti-inflammatory proprieties, and the anti-inflammatory mechanism of P3G involves the arrest of IkB-α activation and reduction in JNKMAPK phosphorylation. The results reinforce that strawberry fruits are functional foods that can act as an adjuvant in the treatment of inflammatory conditions.

Perfil socioeconômico dos feirantes e consumidores da Feira do Produtor de Passo Fundo, RS / Socioeconomic profile of farmers and consumers of the Passo Fundo farmer’s market, Rio Grande de Sul state, Brazil

A Feira do Produtor de Passo Fundo, embora seja um evento institucionalizado há 22 anos, ainda não havia sido objeto de estudo formalizado quanto à caracterização social e econômica de quem nela vende e compra. O objetivo deste trabalho foi traçar o perfil socioeconômico dos feirantes e consumidores da Feira do Produtor de Passo Fundo, RS. Os dados foram obtidos por meio da aplicação de questionário junto a 58 feirantes e 471 consumidores. Os questionários abrangeram questões referentes aos aspectos socioeconômicos dos entrevistados e produtos ofertados/comprados na feira, como hortaliças, frutas, grãos, produtos de origem animal e produtos artesanais. Verificou-se que a faixa etária dos consumidores concentra-se entre 35 a 65 anos. Os feirantes apresentam idade média entre 40 e 55 anos. Um terço dos feirantes possuem núcleo familiar composto por até três pessoas. O nível de escolaridade mais comum neste grupo é o fundamental. Aproximadamente 80 por cento dos entrevistados relataram ganho mensal de até R$1.000,00. Os consumidores são, em sua maioria, indivíduos das classes B2, C e D, dos quais 60 por cento declararam renda mensal entre R$500,00 e R$2.000,00. O valor médio de consumo foi de R$25,94 feira-1. Os homens gastaram, em média, R$26,34 e as mulheres R$25,48 feira-1. As hortaliças e as frutas constituem mais da metade (59 por cento) dos itens comprados na Feira em análise.

Though the Passo Fundo farmer's market has been in existed for over 22 years, the social and economic status of farmers and consumers attended has not been documented yet. Consequently, this study sought to provide the first detailed socioeconomic profile of farmers and consumers that participate in the market. Questionnaires were administered to 58 farmers that hold permanent stalls at the market and another to 471 consumers. The questionnaires had queries regarding the socioeconomic aspects of farmers and consumers, and products offered/purchased at the market, such as, vegetables, fruits, grains, products of animal origin, and handicrafts. While most consumers ranged from 35 to 65 years of age, farmers tended to be between 40 and 55 years old. One-third of farmers had a familiar nucleus composed of up to three people and most had completed elementary school. Nearly 80 percent of farmers reported a monthly income of under 1,000 Brazilian Reais (R$), or roughly $620 US. Typical consumers of the Passo Fundo Farmer's market are of the middle to lower (B2, C and D) social classes. Roughly 60 percent of them declared monthly incomes between 500 and 2000 R$ ($310-1240 US). The average consumption was R$ 25.94 farmer-1 ($16.11 US), with men's mean purchases (R$26.34 farmer-1, $16.41 US) being slightly greater than for women (R$25.48 farmer-1, $15.83 US). Vegetables and fruits made up 59 percent of the items purchased by consumers.