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Removing pesticides from biological drinking water filters is challenging due to the difficulty in activating pesticide-degrading bacteria within the filters. Bioaugmented bacteria can alter the filter's microbiome, affecting its performance either positively or negatively, depending on the bacteria used and their interaction with native microbes. We demonstrate that adding specific bacteria strains can effectively remove recalcitrant pesticides, like metaldehyde, yielding compliance to regulatory standards for an extended period. Our experiments revealed that the Sphingobium CMET-H strain was particularly effective, consistently reducing metaldehyde concentrations to levels within regulatory compliance, significantly outperforming Acinetobacter calcoaceticus E1. This success is attributed to the superior acclimation and distribution of the Sphingobium strain within the filter bed, facilitating more efficient interactions with and degradation of the pesticide, even when present at lower population densities compared to Acinetobacter calcoaceticus E1. Furthermore, our study demonstrates that the addition of pesticide-degrading strains significantly impacts the filter's microbiome at various depths, despite these strains making up less than 1% of the total microbial community. The sequence in which these bacteria are introduced influences the system's ability to degrade pesticides effectively. This research shows the potential of carefully selected and dosed bioaugmented bacteria to improve the pesticide removal capabilities of water filtration systems, while also highlighting the dynamics between bioaugmented and native microbial communities. Further investigation into optimizing bioaugmentation strategies is suggested to enhance the resilience and efficiency of drinking water treatment systems against pesticide contamination.
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Biodegradación Ambiental , Filtración , Microbiota , Plaguicidas , Contaminantes Químicos del Agua , Purificación del Agua , Plaguicidas/metabolismo , Purificación del Agua/métodos , Filtración/métodos , Contaminantes Químicos del Agua/metabolismo , Bacterias/metabolismo , Agua Potable/microbiología , Acinetobacter calcoaceticus/metabolismoRESUMEN
Per- and poly-fluoroalkyl substances (PFAS) have emerged as newly regulated micropollutants, characterised by extreme recalcitrance and environmental toxicity. Constructed wetlands (CWs), as a nature-based solution, have gained widespread application in sustainable water and wastewater treatment and offer multiple environmental and societal benefits. Despite CWs potential, knowledge gaps persist in their PFAS removal capacities, associated mechanisms, and modelling of PFAS fate. This study carried out a systematic literature review, supplemented by unpublished experimental data, demonstrating the promise of CWs for PFAS removal from the influents of varying sources and characteristics. Median removal performances of 64, 46, and 0 % were observed in five free water surface (FWS), four horizontal subsurface flow (HF), and 18 vertical flow (VF) wetlands, respectively. PFAS adsorption by the substrate or plant root/rhizosphere was deemed as a key removal mechanism. Nevertheless, the available dataset resulted unsuitable for a quantitative analysis. Data-driven models, including multiple regression models and machine learning-based Artificial Neural Networks (ANN), were employed to predict PFAS removal. These models showed better predictive performance compared to various mechanistic models, which include two adsorption isotherms. The results affirmed that artificial intelligence is an efficient tool for modelling the removal of emerging contaminants with limited knowledge of chemical properties. In summary, this study consolidated evidence supporting the use of CWs for mitigating new legacy PFAS contaminants. Further research, especially long-term monitoring of full-scale CWs treating real wastewater, is crucial to obtain additional data for model development and validation.
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Ongoing brain activity preceding visual stimulation has been suggested to shape conscious perception. According to the pulsed inhibition framework, bouts of functional inhibition arise in each alpha cycle (every ~100 ms), allowing information to be processed in a pulsatile manner. Consequently, it has been hypothesized that perceptual outcome can be influenced by the specific phase of alpha oscillations prior to the stimulus onset, although empirical findings are controversial. In this study, we aimed to shed light on the role of prestimulus alpha oscillations in visual perception. To this end, we recorded electroencephalographic activity, while participants performed three near-threshold visual detection tasks with different attentional involvement: a no-cue task, a noninformative cue task (50% validity), and an informative cue task (100% validity). Cluster-based permutation statistics were complemented with Bayesian analyses to test the effect of prestimulus oscillatory amplitude and phase on visual awareness. We additionally examined whether these effects differed in trials with low and high oscillatory amplitude, as expected from the pulsed inhibition theory. Our results show a clear effect of prestimulus alpha amplitude on conscious perception, but only when alpha fluctuated spontaneously. In contrast, we did not find any evidence that prestimulus alpha phase influenced perceptual outcome, not even when differentiating between low- and high-amplitude trials. Furthermore, Bayesian analysis provided moderate evidence in favor of the absence of phase effects. Taken together, our results challenge the central theoretical predictions of the pulsed inhibition framework, at least for the particular experimental conditions used here.
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Electroencefalografía , Percepción Visual , Humanos , Teorema de Bayes , Percepción Visual/fisiología , Atención/fisiología , Estimulación Luminosa/métodos , Ritmo alfa/fisiologíaRESUMEN
The removal of EDCs in activated sludge processes can be enhanced by increasing solid and hydraulic retention times (SRT and HRT); it has been suggested that the improvement in removal is due to changes in microbial community structure (MCS). Though the influence of SRT and HRT on chemical removal and MCS has been studied in isolation, their synergistic impact on MCS and the removal of estrogens and nonylphenols in activated sludge remains unknown. Hence, we investigated how both parameters influence MCS in activated sludge processes and their ulterior effect on EDC removal. In our study, an activated sludge pilot-plant was fed with domestic sewage fortified with 100 and 1000 ng/L nonylphenols or 2 and 15 ng/L estrogens and operated at 3, 10 and 27 d SRT (constant HRT) and at 8, 16 and 24 h HRT (constant SRT). The MCS was assessed by phospholipid fatty acids (PLFA) analysis, and the archaeal and bacterial diversities were determined by 16S rRNA analysis. From the PLFA, the microbial abundance ranked as follows: Gram-negative > fungi > Gram-positive > actinomycetes whilst 16S rRNA analysis revealed Proteobacteria > Bacteroidetes > Others. Both PLFA and 16S rRNA analysis detected changes in MCS as SRT and HRT were increased. An SRT increment from 3 to 10 d resulted in higher estrone (E1) removal from 19 to 93% and nonylphenol-4-exthoxylate (NP4EO) from 44 to 73%. These findings demonstrate that EDC-removal in activated sludge plants can be optimised where longer SRT (>10 d) and HRT (>8 h) are suitable. We have also demonstrated that PLFA can be used for routine monitoring of changes in MCS in activated sludge plants.
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There is increasing demand for low-carbon remediation strategies for reducing greenhouse gas emissions and promoting sustainable development in the management of environmental contamination. This trend is within the broader context of sustainable remediation strategies that balance environmental, economic, and social aspects. This article critically reviewed existing literature to evaluate and compare various low-carbon remediation methods, such as bioremediation, phytoremediation, in situ chemical oxidation, soil vapour extraction, and electrokinetic remediation, to identify suitable techniques for the remediation of oil-contaminated sites in the Niger Delta region of Nigeria. We analysed the UK sustainable remediation frameworks (SuRF-UK) to glean lessons for the Nigerian context. Our findings indicate that bioremediation and phytoremediation are particularly promising low-carbon remediation technologies for the Niger Delta region due to their cost-effectiveness and adaptability to local conditions. We proposed a framework that deeply considers opportunities for achieving multiple goals including effective remediation and limited greenhouse gas emissions while returning net social and economic benefit to local communities. The proposed framework will help decision makers to implement effective remediation technologies that meet sustainability indices, integrates emissions considerations return net environmental benefit to local communities. There is a need for policymakers to establish and enforce policies and regulations that support sustainable remediation practises, build the capacity of stakeholders, invest in research and development, and promote collaboration among stakeholders to create a regulatory environment that supports sustainable remediation practises and promotes environmental sustainability in the region. This study provides insights for achieving low-carbon remediation in regions addressing land contamination by different contaminants and facilitates the adoption of remediation technologies that consider contextual socio-economic and environmental indices for sustainable development.
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Coagulant dosing to achieve low phosphorus concentrations in wastewater effluents may favour the removal of trace organics such as pharmaceuticals, plasticisers and flame retardants. Nevertheless, the behaviour of trace organics in coagulation processes is currently poorly understood because of the complex interactions between these compounds, the coagulants and dissolved organic matter (DOM). This study assessed the coagulation removal from synthetic secondary effluent of twenty-four compounds including ten PFAS and four brominated flame retardants. Testing involved two coagulants (alum, ferric chloride) and five DOM surrogates (resorcinol, benzoic acid, citric acid, tannic acid, humic acid); DOM surrogates had assorted molecular weights, structures, charges, and hydrophobicity. With coagulant doses of 14 mg Fe/L and 4 mg Al/L, ten trace organics were removed by >30 % in the presence of at least one DOM surrogate. Humic acid effected the highest removals owing to complexation of trace organics and subsequent co-removal by adsorption or sweep floc. For instance, removal extents for three brominated diphenyl ethers were 60 to 75 % with Al and 50 to 88 % with Fe (initial concentration 0.4 to 0.8 ng/L); PFTDA, a long-chain PFAS, was removed by 87 and 91 % with Fe in the presence of tannic or humic acid, respectively (initial concentration 0.03 µg/L). The varying coagulation performance of different treatment works in terms of trace substance removal can be explained because of the site-specific DOM characteristics. Addition of humic acids as complexing agents has the potential to improve the removal of hydrophobic trace substances, including some long-chain PFAS and brominated flame retardants.
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Introduction: Pattern separation (PS) is a fundamental aspect of memory creation that defines the ability to transform similar memory representations into distinct ones, so they do not overlap when storing and retrieving them. Experimental evidence in animal models and the study of other human pathologies have demonstrated the role of the hippocampus in PS, in particular of the dentate gyrus (DG) and CA3. Patients with mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HE) commonly report mnemonic deficits that have been associated with failures in PS. However, the link between these impairments and the integrity of the hippocampal subfields in these patients has not yet been determined. The aim of this work is to explore the association between the ability to perform mnemonic functions and the integrity of hippocampal CA1, CA3, and DG in patients with unilateral MTLE-HE. Method: To reach this goal we evaluated the memory of patients with an improved object mnemonic similarity test. We then analyzed the hippocampal complex structural and microstructural integrity using diffusion weighted imaging. Results: Our results indicate that patients with unilateral MTLE-HE present alterations in both volume and microstructural properties at the level of the hippocampal subfields DG, CA1, CA3, and the subiculum, that sometimes depend on the lateralization of their epileptic focus. However, none of the specific changes was found to be directly related to the performance of the patients in a pattern separation task, which might indicate a contribution of various alterations to the mnemonic deficits or the key contribution of other structures to the function. Discussion: we established for the first time the alterations in both the volume and the microstructure at the level of the hippocampal subfields in a group of unilateral MTLE patients. We observed that these changes are greater in the DG and CA1 at the macrostructural level, and in CA3 and CA1 in the microstructural level. None of these changes had a direct relation to the performance of the patients in a pattern separation task, which suggests a contribution of various alterations to the loss of function.
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There is broad consensus supporting the reciprocal influence of working memory (WM) and attention. Top-down mechanisms operate to cope with either environmental or internal demands. In that sense, it is possible to select an item within the contents of WM to endow it with prioritized access. Although evidence supports that maintaining an item in this privileged state does not rely on sustained visual attention, it is unknown whether selection within WM depends on perceptual attention. To answer this question, we recorded electrophysiological neural activity while participants performed a retro-cue task in which we inserted a detection task in the delay period after retro-cue presentation. Critically, the onset of to-be-detected near threshold stimuli was unpredictable, and thus, sustained perceptual spatial attention was needed to accomplish the detection task from the offset of the retro-cue. At a behavioral level, we found decreased visual detection when a WM representation was retro-cued. At a neural level, alpha oscillatory activity confirmed a spatial shift of attention to the retro-cued representation. We interpret the convergence of neural oscillations and behavioral data to point towards the theory that selection within WM could be accomplished through a perceptual attentional mechanism.
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Señales (Psicología) , Memoria a Corto Plazo , Humanos , Memoria a Corto Plazo/fisiología , Consenso , Percepción Visual/fisiologíaRESUMEN
Two main explanations for memory loss have been proposed. On the one hand, decay theories consider that over time memory fades away. On the other hand, interference theories sustain that when similar memories are encoded, they become more prone to confusion. The interference is greater as the degree of similarity between memories increases, and as the number of similar traces increases too. To reduce interference, the pattern separation process allows the brain to separate similar memories and build detailed memory representations that are less easily confused. Nonetheless, with time, we tend to remember more general aspects of experiences, which also affects our ability to discriminate. We present the results of one experiment in which brain activity was recorded by EEG while two groups of healthy participants performed a visual memory discrimination task. This task assesses the ability to differentiate new but similar information from previously learned information and thus avoid interference. Unlike previous studies, we used a paradigm that was specifically designed to assess the impact of the number of items (2 or 6) of each category stored in memory, as well as the time elapsed after the study phase (20 min or 24 h), on recognition memory for objects. Behaviorally, our results suggest that mnemonic discrimination is not modulated by the passage of time, but by the number of stored events. ERP results show a reduced amplitude in posterior regions between 500 and 700 ms when comparing short and long delays. We also observe a more positive activity in a centro-posterior region in the 500-700 ms window at retrieval when participants store more items. Interestingly, amplitudes for old hits and similar false alarms were greater than amplitudes for correctly rejected new items between 500 and 700 ms. This finding indicates that a recollection-based process operates in both true and false recognition. We also found that the waveforms for correct rejections of similar lures and the waveforms for correct rejections of new items were comparable.
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A novel polyaniline (PANI) coupled CuBi2O4 photocatalyst was successfully synthesized via in situ polymerization of aniline with pre-synthesized CuBi2O4 composites. The structure and morphology of the synthesized CuBi2O4/PANI composite photocatalyst were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) and the photocatalytic performance were evaluated through degradation process of ammonia in water under visible light irradiation. The resultant CuBi2O4/PANI composite showed exceptional stability as its structure and morphology persisted even after being immersed in water for 2 days. The composite photocatalyst exhibited improved charge transport properties due to the electrical conductivity of the PANI protective layer, leading to enhanced photoelectrochemical activity in water and removal of ammonia. PANI with CuBi2O4 (10% wt) heterostructure was applied for photodegradation of ammonia and exhibited a 96% ammonia removal efficiency (30 mg/l with 0.1 g photocatalyst and 180 min), as compared to PANI (78%) and CuBi2O4 (70%). The degradation was attributed to the efficient charge transfer (e- and h+) and formation of reactive oxygen species upon simulated sunlight exposure. The present work suggests that the CuBi2O4/PANI photocatalyst can be synthesized in a simple process and provides an excellent adsorption capacity, high photocatalytic activity, long term stability, and reusability making it a promising alternative for ammonia removal from wastewater.
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It has been proposed that alpha oscillations reflect the endogenous modulation of visual cortex excitability. In particular, alpha power increases during the maintenance period in Working Memory (WM) tasks have been interpreted as a mechanism to avoid potential interference of incoming stimuli. In this study we tested whether alpha power was modulated during the maintenance of WM to enhance the processing of relevant incoming perceptual stimuli. To this aim, we manipulated the contrast of a stimulus presented during the maintenance period of a WM task. The to-be-detected stimulus could indicate which of the encoded representations was going to be probed after the delay (spatial retro-cue) or could signal that all the representations had equal probability to be tested (neutral retro-cue). Time-frequency analysis revealed that alpha power preceding retro-cue presentation was not differently modulated by the two different contrast conditions. This is, participants did not endogenously modulate alpha oscillations upon low perceptual contrast stimuli incoming. These results suggest that alpha delay activity is not a goal directed mechanism to control the inflow of information during WM maintenance. Instead, current data suggest that alpha delayed activity might be an index of increased allocation of attentional resources to the processing of the WM representations.
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Señales (Psicología) , Corteza Visual , Atención , Humanos , Memoria a Corto Plazo , Percepción VisualRESUMEN
Brain oscillations are considered to play a pivotal role in neural communication. However, detailed information regarding the typical oscillatory patterns of individual brain regions is surprisingly scarce. In this study we applied a multivariate data-driven approach to create an atlas of the natural frequencies of the resting human brain on a voxel-by-voxel basis. We analysed resting-state magnetoencephalography (MEG) data from 128 healthy adult volunteers obtained from the Open MEG Archive (OMEGA). Spectral power was computed in source space in 500 ms steps for 82 frequency bins logarithmically spaced from 1.7 to 99.5 Hz. We then applied k-means clustering to detect characteristic spectral profiles and to eventually identify the natural frequency of each voxel. Our results provided empirical confirmation of the canonical frequency bands and revealed a region-specific organisation of intrinsic oscillatory activity, following both a medial-to-lateral and a posterior-to-anterior gradient of increasing frequency. In particular, medial fronto-temporal regions were characterised by slow rhythms (delta/theta). Posterior regions presented natural frequencies in the alpha band, although with differentiated generators in the precuneus and in sensory-specific cortices (i.e., visual and auditory). Somatomotor regions were distinguished by the mu rhythm, while the lateral prefrontal cortex was characterised by oscillations in the high beta range (>20 Hz). Importantly, the brain map of natural frequencies was highly replicable in two independent subsamples of individuals. To the best of our knowledge, this is the most comprehensive atlas of ongoing oscillatory activity performed to date. Critically, the identification of natural frequencies is a fundamental step towards a better understanding of the functional architecture of the human brain.
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Magnetoencefalografía , Descanso , Adulto , Encéfalo , Mapeo Encefálico/métodos , Humanos , Magnetoencefalografía/métodos , Lóbulo TemporalRESUMEN
This review compiles recent advances and challenges on photocatalytic treatment of wastewater using nanoparticles, nanocomposites, and polymer nanocomposites as photocatalyst. The review provides an overview of the fundamental principles of photocatalytic treatment along the recent advances on photocatalytic treatment, especially on the modification strategies and operational conditions to enhance treatment efficiency and removal of recalcitrant organic contaminants. The different types of photocatalysts along the key factors influencing their performance are also critically discussed and recommendations for future research are provided.
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This study evaluated the effects of three commercial dispersants (Finasol OSR 52, Slickgone NS, Superdispersant 25) and three biosurfactants (rhamnolipid, trehalolipid, sophorolipid) in crude-oil seawater microcosms. We analysed the crucial early bacterial response (1 and 3 days). In contrast, most analyses miss this key period and instead focus on later time points after oil and dispersant addition. By focusing on the early stage, we show that dispersants and biosurfactants, which reduce the interfacial surface tension of oil and water, significantly increase the abundance of hydrocarbon-degrading bacteria, and the rate of hydrocarbon biodegradation, within 24 h. A succession of obligate hydrocarbonoclastic bacteria (OHCB), driven by metabolite niche partitioning, is demonstrated. Importantly, this succession has revealed how the OHCB Oleispira, hitherto considered to be a psychrophile, can dominate in the early stages of oil-spill response (1 and 3 days), outcompeting all other OHCB, at the relatively high temperature of 16 °C. Additionally, we demonstrate how some dispersants or biosurfactants can select for specific bacterial genera, especially the biosurfactant rhamnolipid, which appears to provide an advantageous compatibility with Pseudomonas, a genus in which some species synthesize rhamnolipid in the presence of hydrocarbons.
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Microporous zeolite-type materials, with crystalline porous structures formed by well-defined channels and cages of molecular dimensions, have been widely employed as heterogeneous catalysts since the early 1960s, due to their wide variety of framework topologies, compositional flexibility and hydrothermal stability. The possible selection of the microporous structure and of the elements located in framework and extraframework positions enables the design of highly selective catalysts with well-defined active sites of acidic, basic or redox character, opening the path to their application in a wide range of catalytic processes. This versatility and high catalytic efficiency is the key factor enabling their use in the activation and conversion of different alkanes, ranging from methane to long chain n-paraffins. Alkanes are highly stable molecules, but their abundance and low cost have been two main driving forces for the development of processes directed to their upgrading over the last 50 years. However, the availability of advanced characterization tools combined with molecular modelling has enabled a more fundamental approach to the activation and conversion of alkanes, with most of the recent research being focused on the functionalization of methane and light alkanes, where their selective transformation at reasonable conversions remains, even nowadays, an important challenge. In this review, we will cover the use of microporous zeolite-type materials as components of mono- and bifunctional catalysts in the catalytic activation and conversion of C1+ alkanes under non-oxidative or oxidative conditions. In each case, the alkane activation will be approached from a fundamental perspective, with the aim of understanding, at the molecular level, the role of the active sites involved in the activation and transformation of the different molecules and the contribution of shape-selective or confinement effects imposed by the microporous structure.
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Decades of research have increased the understanding of the contribution of the anterior temporal lobes (ATLs) to semantic cognition. Nonetheless, whether semantic processing of different types of information show a selective relationship with left and right ATLs, or whether semantic processing in the ATLs is independent of the modality of the input is currently unknown. There exists evidence supporting each of these alternatives. A fundamental objection to these findings is that they were obtained from studies with patients with brain damage affecting extensive regions, sometimes bilaterally. In the current study, we assessed a group of 38 temporal lobe epilepsy (TLE) patients with either left or right small epileptogenic lesions with a battery of commonly used semantic tasks that tested verbal and non-verbal semantic processing. We found that left TLE patients exhibited worse performance than controls on the verbal semantic tasks, as expected, but also on the non-verbal semantic task. On the other hand, performance of the right TLE group did not differ from controls on the non-verbal task, but was worse on a semantic fluency task. When performance between patient groups was compared, we found that left TLE not only did worse than right TLE on the naming task, but also on the non-verbal associative memory task. When considered together, current data do not support a strong view of input modality differences between left and right ATLs. Additionally, they provide evidence indicating that the left and right ATLs do not make similar contributions to a singular functional system for semantic representation.
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Epilepsia del Lóbulo Temporal , Semántica , Cognición , Epilepsia del Lóbulo Temporal/complicaciones , Lateralidad Funcional , Humanos , Imagen por Resonancia Magnética , Pruebas Neuropsicológicas , Lóbulo Temporal/diagnóstico por imagen , Conducta VerbalRESUMEN
PURPOSE: We hypothesized that epilepsy associated with temporal pole encephaloceles (ETPE) could be the consequence and an unrecognized manifestation of idiopathic intracranial hypertension (IIH). To test this hypothesis in patients with ETPEs we evaluated: 1) the frequency of two radiological signs of IIH and 2) whether these patients develop over time clinical manifestations suggestive of elevated intracranial pressure (ICP). METHODS: Case-control study comparing two cardinal radiological signs of IIH pituitary gland height (PGH) and the diameter of the two optic nerve sheaths (ONS) between 29 patients with ETPEs (TPE group) and 29 patients with focal epilepsy of other etiologies (control group), adjusted by age, sex, body mass index (BMI), age at epilepsy onset and epilepsy duration. Analysis was performed using conventional and ordinal logistic regression. The measurements in both groups were compared with validated radiological criteria of IIH. RESULTS: Of the patients 17 (63%) in the TPE group had all three measurements over the cut-off values for IIH, while no patients in the control group had all three findings. The TPE group patients had lower PGH (3.2⯱ 1.0â¯mm vs. 4.9⯱ 1.3â¯mm, pâ¯< 0.001) and larger diameter of ONS than controls (pâ¯< 0.001), being similar to validated data of IIH. No patient with TPE had clinical manifestations of elevated ICP (mean follow-up 15.1⯱ 11.7 years). CONCLUSION: Patients with ETPEs frequently had radiological signs of IIH while not developing typical manifestations of elevated ICP over time. In this way, ETPEs could be an unrecognized manifestation of IIH, and temporal lobe seizures the only clinical expression of this epilepsy syndrome.
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Epilepsia , Seudotumor Cerebral , Estudios de Casos y Controles , Encefalocele/diagnóstico por imagen , Humanos , Lóbulo TemporalRESUMEN
In September 2017 the Agia Zoni II sank in the Saronic Gulf, Greece, releasing approximately 500 tonnes of heavy fuel oil, contaminating the Salamina and Athens coastlines. Effects of the spill, and remediation efforts, on sediment microbial communities were quantified over the following 7 months. Five days post-spill, the concentration of measured hydrocarbons within surface sediments of contaminated beaches was 1,093-3,773 µg g-1 dry sediment (91% alkanes and 9% polycyclic aromatic hydrocarbons), but measured hydrocarbons decreased rapidly after extensive clean-up operations. Bacterial genera known to contain oil-degrading species increased in abundance, including Alcanivorax, Cycloclasticus, Oleibacter, Oleiphilus, and Thalassolituus, and the species Marinobacter hydrocarbonoclasticus from approximately 0.02 to >32% (collectively) of the total bacterial community. Abundance of genera with known hydrocarbon-degraders then decreased 1 month after clean-up. However, a legacy effect was observed within the bacterial community, whereby Alcanivorax and Cycloclasticus persisted for several months after the oil spill in formerly contaminated sites. This study is the first to evaluate the effect of the Agia Zoni II oil-spill on microbial communities in an oligotrophic sea, where in situ oil-spill studies are rare. The results aid the advancement of post-spill monitoring models, which can predict the capability of environments to naturally attenuate oil.