Assessing the inconsistency of microplastic measurements in foods and beverages.

The widespread occurrence of microplastics (MPs) in the food chain has gained substantial recognition as a pressing concern, highlighting the inevitability of human exposure through ingestion of foodborne MPs, coupled with the release of MPs from plastic packaging. However, there are notable disparities in the reported numbers of MPs in foods and beverages, warranting a thorough investigation into the factors contributing to these discrepancies. Table salt is one of the major sources of MPs, and there was an approximately hundred-fold difference between the reviewed studies that reported the highest and lowest number of MPs. In addition, more noticeable discrepancies were discovered between studies on MPs released from teabags. One study reported that approximately 15 billion MPs were released into a cup of tea from a single teabag, whereas another research paper found only approximately 106.3 ± 14.6 MP/teabag after brewing. This comprehensive review focuses on the inconsistencies observed across studies examining MPs, shedding light on the plausible factors underlying these variations. Furthermore, the review outlines areas in analytical procedures that require enhancement and offers recommendations to promote accuracy and standardization in future research efforts, such as employing analytical methods capable of confirming the presence of MPs, using appropriate filter sizes, considering representative sample sizes when extrapolation is involved, and so on. By pinpointing the detection processes leading to the inconsistent results observed in MP studies, this comparative analysis will contribute to the development of reliable analytic methods for understanding the extent of microplastic contamination in the human food chain.

Improved digestive stability of probiotics encapsulated within poly(vinyl alcohol)/cellulose acetate hybrid fibers.

Novel cellulose acetate (CA) and poly(vinyl alcohol) (PVA) hybrid fibers, fabricated via angled dual-nozzle electrospinning, were used for the encapsulation of probiotics to enhance their gastrointestinal stability. In this study, Escherichia coli strain Nissle 1917 (EcN) cells were encapsulated within PVA/CA composite mats, where CA enhanced the bacterial stability under gastric conditions and PVA provided protection against the toxic solvent during the electrospinning process. Scanning electron microscopy images revealed that EcN was successfully encapsulated within the hybrid fibers. In the simulated digestive system, free cells lost their viability within 100 min, whereas PVA/CA-encapsulated cells survived with a final count of 3.9 log CFU/mL (from an initial count of 7.8 log CFU/mL), an increase of 1 log CFU/mL compared with those in PVA/PVA fibers. Considering the enhanced viability of the encapsulated cells in the gastrointestinal system, multi-nozzle electrospinning is a promising technique for the fabrication of novel matrices for probiotic encapsulation.