Estimation of stature and body weight from static and dynamic footprints - Forensic implications and validity of non-colouring cream method.

In the present study, the metric properties of dynamic footprints were analysed using non-colouring method in relation with body parameters and compared with static footprint measurements. The results of the study provide a better understanding of the relationship between static and dynamic footprints, which may be useful for biological profiling that allows more accurate identification. Stature, body weight, five length and two width parameters of dynamic (walking) footprints of young Slovak adults (65 females and 68 males) were analysed. Pearson correlation coefficients were calculated and equations for prediction of stature and body weight by linear regression analysis and multiple regression analysis were developed. Intersex differences were confirmed for all parameters and bilateral for some. Statistically significant differences were found in all measurements (p-value>0.05), except for the width of the standing and walking footprint in the mixed group. A positive correlation was found between the selected footprint diameters with stature (max - r = 0.82) and body weight (max - r = 0.70). Stature could be calculated with an accuracy of up to 4.40 cm and body weight with an accuracy of up to 9.56 kg. The results of the present study show that selected measurements of dynamic footprints correlate with stature and body weight. These results could be used in biological profiling in the medical and forensic fields.

Technical Note: The Forensic Anthropology Society of Europe (FASE) Map of Identified Osteological Collections.

Identified (documented) osteological collections represent an important resource in the development of forensic anthropology standards and methods as well as a precious tool for learning and training of practitioners. Even though the number of papers presenting identified collections worldwide increases, many of the collections have still not been divulged to the scientific community in sufficient detail to ascertain their exact number. The Forensic Anthropology Society of Europe (FASE) therefore developed a tool that goes beyond sporadic publications: the FASE Map of Identified Osteological Collections, which is freely accessible and continuously updated and revised. The online map is available at http://forensicanthropology.eu/osteological-collections/. The map of skeletal collections was created in 2017 and currently displays information on 153 identified osteological collections (43 of them categorized as contemporary) located in 41 different countries. This article offers a short analysis of the type, geographical location and content of the collections included in the map. The aim of this article and the map as such is to provide a useful resource to facilitate research planning and teaching in forensic anthropology and related disciplines.

Comparison of microbial diversity during two different wine fermentation processes.

Wine production is a complex procedure in which an important role is played by many microorganisms, particularly yeasts and bacteria. In modern wineries, alcoholic fermentation is usually carried out by adding microbial starter cultures of Saccharomyces cerevisiae strains for precisely controlled production. Nowadays, in the Slovak Republic, autochthonous vinification is getting more popular. The present article deals with the comparison of two vinification approaches, namely spontaneous fermentation and fermentation controlled by a standard commercial S. cerevisiae starter, from the point of view of microbiota dynamics and the chemical characteristics of the wines produced. The dynamics of microbial populations were determined during the fermentation process by a 16S and 28S rRNA next-generation sequencing approach. A profile of the volatile compounds during these fermentation processes was identified by solid-phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS). In summary, the microbial diversity in the m1 phase (initial must) was higher, despite the presence of the starter culture. In the m3 phase (young wine), the microbiome profiles of both batches were very similar. It seems that the crucial phase in order to study the relationship of the microbiome and the resulting product should be based on the m2 phase (fermented must), where the differences between the autochthonous and inoculated batches were more evident.

Validation of a low-cost laser scanner device for the assessment of three-dimensional facial anatomy in living subjects.

The present study compared the reliability of a low-cost laser scanner device to an already-validated stereophotogrammetric instrument. Fifty volunteers underwent duplicate facial scans through laser scanner and stereophotogrammetry. Intra- and inter-instrument reproducibility of linear distances, angles, facial surface area and volume was verified through the Bland-Altman test and calculation of absolute (TEM) and relative (rTEM) technical errors of measurement; rTEM was then classified as follows: <1% excellent; 1-3.9% very good; 4-6.9% good; 7-9.9% moderate; >10% poor. The scans performed through different devices were registered and superimposed to calculate the root mean square (RMS) (point-to-point) distance between the two surfaces. The same protocol was applied to a mannequin head. In inter-instruments comparison, 12/26 measurements showed a "good" rTEM; 5 were "very good". In intra-instrument comparison, most performances worsened, with only 10 of 26 measurements classified as "good" and "very good". All the measurements made on mannequin scans were at least "good", and 14/26 were "very good". Surface area was "very good" only in intra-instrument comparison; conversely, volumes were poorly repeatable for all the comparisons. On average, RMS point-to-point distances were 0.65 mm (inter-devices comparison), 0.56 mm (mannequin scans), 0.42 mm (intra-device comparison). In conclusion, the low-cost laser scan device can be reliably applied to inanimate objects, but does not meet the standards for three-dimensional facial acquisition on living persons.

Estimation of stature and body weight in Slovak adults using static footprints: A preliminary study.

The stature and the body weight as part of the biological profile can aid the personal identification. The dimensions of the human foot, as well as the footprint, can be used for the prediction due to the existence of its positive correlation with the stature and body weight. Five diagonal axes and ball breadth of bilateral static footprints of 132 young Slovak adults were obtained. All diameters were larger in a male group than female group. No bilateral differences were found except the first diagonal axis and ball breadth. A positive correlations between the selected footprint diameters with the stature (r = 0.37-0.64) and the body weight (r = 0.29-0.71) were confirmed. The linear and multiple regression prediction equations were developed. A stature prediction equation using the most lateral diameters (the fourth and fifth diagonal axis) exhibited the highest accuracy ranging from 4 to 7.5 cm. Similar results were found for the body weight estimation of the male and mixed group. In the female group, the most medial axis (first and second) exhibited the highest accuracy. The body weight estimation accuracy ranges from 9.09 to 11.09 kg. The real and predicted stature and body weight were compared and found differences were lower than calculated SEEs. Thresholds and prediction trend of under- or overestimation was identified. The results of the present study show that selected measurements of static footprints could be used to predict stature and body weight but should be applied only for Slovaks due to population specificity.

Personal Identification of Deceased Persons: An Overview of the Current Methods Based on Physical Appearance.

The use of the physical appearance of the deceased has become more important because the available antemortem information for comparisons may consist only of a physical description and photographs. Twenty-one articles dealing with the identification based on the physiognomic features of the human body were selected for review and were divided into four sections: (i) visual recognition, (ii) specific facial/body areas, (iii) biometrics, and (iv) dental superimposition. While opinions about the reliability of the visual recognition differ, the search showed that it has been used in mass disasters, even without testing its objectivity and reliability. Specific facial areas being explored for the identification of dead; however, their practical use is questioned, similarly to soft biometrics. The emerging dental superimposition seems to be the only standardized and successfully applied method for identification so far. More research is needed into a potential use of the individualizing features, considering that postmortem changes and technical difficulties may affect the identification.

3D quantitative analysis of early decomposition changes of the human face.

Decomposition of the human body and human face is influenced, among other things, by environmental conditions. The early decomposition changes that modify the appearance of the face may hamper the recognition and identification of the deceased. Quantitative assessment of those changes may provide important information for forensic identification. This report presents a pilot 3D quantitative approach of tracking early decomposition changes of a single cadaver in controlled environmental conditions by summarizing the change with weekly morphological descriptions. The root mean square (RMS) value was used to evaluate the changes of the face after death. The results showed a high correlation (r = 0.863) between the measured RMS and the time since death. RMS values of each scan are presented, as well as the average weekly RMS values. The quantification of decomposition changes could improve the accuracy of antemortem facial approximation and potentially could allow the direct comparisons of antemortem and postmortem 3D scans.

Recognition of children on age-different images: Facial morphology and age-stable features.

The situation of missing children is one of the most emotional social issues worldwide. The search for and identification of missing children is often hampered, among others, by the fact that the facial morphology of long-term missing children changes as they grow. Nowadays, the wide coverage by surveillance systems potentially provides image material for comparisons with images of missing children that may facilitate identification. The aim of study was to identify whether facial features are stable in time and can be utilized for facial recognition by comparing facial images of children at different ages as well as to test the possible use of moles in recognition. The study was divided into two phases (1) morphological classification of facial features using an Anthropological Atlas; (2) algorithm developed in MATLAB® R2014b for assessing the use of moles as age-stable features. The assessment of facial features by Anthropological Atlases showed high mismatch percentages among observers. On average, the mismatch percentages were lower for features describing shape than for those describing size. The nose tip cleft and the chin dimple showed the best agreement between observers regarding both categorization and stability over time. Using the position of moles as a reference point for recognition of the same person on age-different images seems to be a useful method in terms of objectivity and it can be concluded that moles represent age-stable facial features that may be considered for preliminary recognition.

The Reliability of Facial Recognition of Deceased Persons on Photographs.

In humanitarian emergencies, such as the current deceased migrants in the Mediterranean, antemortem documentation needed for identification may be limited. The use of visual identification has been previously reported in cases of mass disasters such as Thai tsunami. This pilot study explores the ability of observers to match unfamiliar faces of living and dead persons and whether facial morphology can be used for identification. A questionnaire was given to 41 students and five professionals in the field of forensic identification with the task to choose whether a facial photograph corresponds to one of the five photographs in a lineup and to identify the most useful features used for recognition. Although the overall recognition score did not significantly differ between professionals and students, the median scores of 78.1% and 80.0%, respectively, were too low to consider this method as a reliable identification method and thus needs to be supported by other means.