Effect of oil extraction assisted by ultrasound on the physicochemical properties and fatty acid profile of pumpkin seed oil (Cucurbita pepo).

The effects of amplitude and time of ultrasound-assisted extraction on the physicochemical properties and the fatty acid profile of pumpkin seed oil (Cucurbita pepo) were evaluated. Ultrasound time (5-30 min) and the response variables amplitude (25-100%), extraction yield, efficiency, oxidative stability in terms of the free fatty acids (FFA) of the plant design comprising two independent experiments variables, peroxide (PV), p-anisidine (AV), totox value (TV) and the fatty acid profile were evaluated. The results were analyzed by multiple linear regression. The time and amplitude showed significant differences (P<0.05) for all variables. The highest yield of extraction was achieved at 5 min and amplitude of 62.5% (62%). However, the optimal ultrasound-assisted extraction conditions were as follows: ultrasound time of 26.34 min and amplitude of 89.02%. All extracts showed low FFA (2.75-4.93% oleic acid), PV (1.67-4.68 meq/kg), AV (1.94-3.69) and TV (6.25-12.55) values. The main fatty acids in all the extracts were oleic and linoleic acid. Therefore, ultrasound-assisted oil extraction had increased performance and reduced extraction time without affecting the oil quality.

Impact of micronized starfruit (Averrhoa carambola L.) fiber concentrate on lipid metabolism in mice.

The objective of this study was to evaluate the effect of micronized insoluble fiber from starfruit bagasse as an ingredient of a functional food (FF) or as micronized insoluble fiber-rich fraction (IFRF) and its effects in vivo on lipids metabolism in a murine model. Experimental animals were divided in four isoproteic (15.8%) treatments differing on the fiber and cholesterol level used. The micronized IFRF particle size ranged from 37.5 to 149 µm. Treatments with added IFRF and those including the FF lowered serum triacylglycerols, total cholesterol (TC), high-density lipoproteins (HDL), and low-density lipoproteins (LDL) concentrations (IFRF: 14.2, 25.4, 55.06, and 12.18%, respectively; FF: 30.18, 39.47, 35.11, and 43.18%, respectively). IFRF produced the overall highest serum hypolipidemic effect and prevented the development of non-alcoholic fatty liver. Both the IFRF and the FF exhibited hypolipidemic effects that suggest a potential role of starfruit insoluble fiber as a component of FFs aimed against cardiovascular diseases.

Resistant starch and in vitro starch digestibility of cooked "ayocote" bean Pphaseolus Coccineous)

Se estudió la composición química y digestibilidad in vitro del frijol "ayocote" cocido (Phaseolus coccineous). El contenido de proteína y cenizas fue de 20,46 y 1,39%, respectivamente, los cuales se encuentran entre los más bajos reportados para el género Phaseolus. En el caso de los lípidos su contenido fue relativamente alto (3,31%). Los valores de almidón disponible (AD) disminuyeron con el tiempo de almacenamiento, variando desde 37,93% (muestra recién cocinada) hasta 32,18% (muestra almacenada por 96h). El comportamiento inverso se encontró para el contenido de almidón resistente (AR). Los valores estuvieron entre 2,24% (muestra control) y 3,49% (muestra almacenada por 96h), lo cual representa un incremento del 56% en el contenido de AR. El almidón resistente asociado a la fibra dietética (ARAF) presentó un comportamiento similar, ya que los valores se incrementaron con el tiempo de almacenamiento. La velocidad de a-amilólisis disminuyó con el tiempo de almacenamiento; por ejemplo, las muestras almacenadas por 96h mostraron una velocidad de digestión lenta. El índice glucémico estimado estuvo entre 62,9 y 59,7%, sugiriendo que el almidón del frijol "ayocote" libera muy lentamente la glucosa cuando es hidrolizado por las enzimas digestivas.

"Ayocote" beans (Phaseolus coccineous) were cooked and studied regarding their chemical composition and in vitro starch digestibility. Protein and ash contents were 20.46 and 1.39%, respectively, which are among the lowest levels for seeds of the Phaseolus genus. On the contrary, lipid content was relatively high (3.31%). Available starch (AS) values decreased with storage at 4ºC, changing from 37.93 (freshly cooked "control" seeds) to 32.18% (seeds stored for 96h). An inverse pattern was found for resistant starch (RS) content. RS ranged between 2.24 and 3.49% for the control and 96h-stored samples, which represents a 56% increase in the RS content. Resistant starch associated to dietary fiber (RSAF) had a similar behavior, as its values increased with the storage time. The a-amylolysis rate decreased with storage, i.e. long-stored (96h) cooked samples exhibited slower starch digestion features. The predicted glycemic index ranged between 62.9 and 59.7%, suggesting slow glucose-release features for starch in "ayocote" beans.

Estudou-se a composição química e digestibilidade in vitro do feijão "ayocote" cozido (Phaseolus coccineous). O conteúdo de proteína e cinzas foi de 20,46 e 1,39%, respectivamente, os quais se encontram entre os mais baixos reportados para o gênero Phaseolus. No caso dos lípidos seu conteúdo foi relativamente alto (3,31%). Os valores de amido disponível (AD) diminuíram com o tempo de armazenamento, variando desde 37,93% (amostra recém cozida) até 32,18% (amostra armazenada por 96h). O comportamento inverso encontrou-se para o conteúdo de amido resistente (AR). Os valores estiveram entre 2,24% (amostra controle) e 3,49% (amostra armazenada por 96h), o qual representa um incremento de 56% no conteúdo de AR. O amido resistente associado à fibra dietética (ARAF) apresentou um comportamento similar, já que os valores se incrementaram com o tempo de armazenamento. A velocidade de a-amilólisis diminuiu com o tempo de armazenamento; por exemplo, as amostras armazenadas por 96h mostraram uma velocidade de digestão lenta. O índice glicêmico estimado esteve entre 62,9 e 59,7%, sugerindo que o amido do feijão "ayocote" libera muito lentamente a glicose quando é hidrolisada pelas enzimas digestivas.