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1.
Front Plant Sci ; 13: 1034419, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36466235

RESUMEN

Both plant- and rhizobia-derived small RNAs play an essential role in regulating the root nodule symbiosis in legumes. Small RNAs, in association with Argonaute proteins, tune the expression of genes participating in nodule development and rhizobial infection. However, the role of Argonaute proteins in this symbiosis has been overlooked. In this study, we provide transcriptional evidence showing that Argonaute5 (AGO5) is a determinant genetic component in the root nodule symbiosis in Phaseolus vulgaris. A spatio-temporal transcriptional analysis revealed that the promoter of PvAGO5 is active in lateral root primordia, root hairs from rhizobia-inoculated roots, nodule primordia, and mature nodules. Transcriptional analysis by RNA sequencing revealed that gene silencing of PvAGO5 affected the expression of genes involved in the biosynthesis of the cell wall and phytohormones participating in the rhizobial infection process and nodule development. PvAGO5 immunoprecipitation coupled to small RNA sequencing revealed the small RNAs bound to PvAGO5 during the root nodule symbiosis. Identification of small RNAs associated to PvAGO5 revealed miRNAs previously known to participate in this symbiotic process, further supporting a role for AGO5 in this process. Overall, the data presented shed light on the roles that PvAGO5 plays during the root nodule symbiosis in P. vulgaris.

2.
Parasitol Res ; 119(10): 3523-3529, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-32572573

RESUMEN

The cattle tick Rhipicephalus microplus has a large impact on cattle production due to its bloodsucking habit and transmission of pathogens that cause babesiosis and anaplasmosis. Application of acaricides constitutes the major control method but is often accompanied by serious drawbacks, including environmental contamination and an increase in acaricide resistance by ticks. The recent development of anti-tick vaccines has provided positive results in the post-genomic era, owing to the rise of reverse vaccinological and bioinformatics approaches to analyze and identify candidate protective antigens for use against ticks. The ATAQ protein is considered a novel antigen for the control of the cattle tick R. microplus; it is expressed in midguts and Malpighian tubules of all ticks from the Rhipicephalus genus. However, genetic diversity studies are required. Here, the ATAQ gene was sequenced of seven R. microplus tick isolates from different regions in Mexico to understand the genetic diversity. The results showed that sequence identity among the Mexican isolates ranged between 98 and 100% and 97.8-100% at the nucleotide and protein levels, respectively. Alignments of deduced amino acid sequences from different R. microplus ATAQ isolates in Mexico revealed a high degree of conservation. However, the Mexican isolates differed from the R. microplus "Mozambique" strain, at 20 amino acid residues. Finally, the analysis of more R. microplus isolates, and possibly of other Rhipicephalus species, to determine the genetic diversity in the ATAQ locus is essential to suggest this antigen as a vaccine candidate that might control tick infestations.


Asunto(s)
Proteínas de Artrópodos/genética , Enfermedades de los Bovinos/prevención & control , Rhipicephalus/inmunología , Infestaciones por Garrapatas/veterinaria , Vacunas/inmunología , Secuencia de Aminoácidos , Animales , Proteínas de Artrópodos/inmunología , Bovinos , Variación Genética , México , Rhipicephalus/genética , Alineación de Secuencia , Infestaciones por Garrapatas/prevención & control , Vacunas/administración & dosificación
3.
Psychol. av. discip ; 4(2): 47-56, jul.-dic. 2010. ilus, tab
Artículo en Español | LILACS | ID: lil-669136

RESUMEN

La presente investigación pretendió realizar un primer acercamiento de tipo exploratorio frente a las transformaciones que se han dado en el concepto de conyugalidad en personas pertenecientes a las últimas tres generaciones. Para la realización del proyecto se empleó como instrumento la entrevista a profundidad a través de la cual se hallaron resultados que indican que la conyugalidad ha sufrido grandes trasformaciones desde el imaginario de cada individuo, donde los avances de la medicina y la ocupación de la misma en el cuerpo de la mujer, el cuidado de los hijos y la reproducción, los cambios en el derecho jurídico de la participación femenina en asuntos que eran exclusivamente masculinos, las revoluciones de los años setenta y la inmigración de las familias del campo a los centros urbanos entre otras, han influenciado el comportamiento individual, ocasionando transformaciones en la forma como se enfrentan y se asumen los diversos aconteceres de la vida humana.


This research aimed at an initial exploratory approach, given the changes that have occurred in the concept of marital persons belonging to the past three generations. To achieve the project was used as a tool indepth interviews through which results were found indicating that conjugal has undergone major transformations since the way each individual, where advances in medicine and the occupation of the same in the body of women, child care and reproduction, changes in the legal right of women's participation in matters that were exclusively male, the revolutions of the 70s and the migration of rural families to urban centers among others, have influenced individual behavior, causing changes in the way they are facing and will assume the various happenings of human life.


Asunto(s)
Cambio Social , Conducta , Familia/psicología , Composición Familiar , Relaciones Interpersonales , Familia , Empleo
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