Assessing the Efficacy of Monthly 308 nm Excimer Lamp Double-Stacked Pulse Adjusted Therapy for Alopecia Areata.

Introduction: Alopecia areata (AA) is an autoimmune disease characterized by T cell-mediated attack on the hair follicle. Although there are a wide range of therapies, the majority of them are not satisfactory due to side effects, pain due to intralesional injections or limited efficacy. In this study, we sought to evaluate the efficacy, influence factors, and safety of 308 nm excimer lamp used in a monthly basis in a double-stacked pulse manner for the treatment of AA. Methods: This was a prospective study, using 308 nm excimer lamp in a double-stacked pulse therapy for AA. The primary endpoint was the improvement in SALT score. Results: A total of 40 patients with AA were enrolled in this study. Forty (100%) patients achieved clinical response. Hyperpigmentation and erythema occurred on the treated alopecic areas of all patients but they were considered tolerable. Patients of younger age or with a smaller area of affection had a better overall treatment response. Conclusion: 308 nm excimer lamp therapy is an excellent option for single or multiple AA because it achieves a good clinical response with less adverse effects than other therapies. This therapy may be useful for low-income countries where new JAK inhibitors are not available, however, for patients with extensive hair loss, it is not as effective and thus, it may be unfit for patients with alopecia totalis and alopecia universals.

Strain diversity of Rickettsia amblyommatis in ticks infesting birds in the North Huetar conservation area of Costa Rica.

Although the presence of rickettsial agents in ticks infesting wild birds in Costa Rica has been recently reported, information on strain diversity is limited to selected rickettsial species. In order to mine deeper into rickettsial agents of ticks infesting Costa Rica wild birds a total of 399 birds from the North Huetar Conservation Area of Costa Rica were captured, and 134 immature ticks (76 larvae and 58 nymphs) were recovered from 61 birds. Ticks were tested for the presence of Rickettsia spp. by conventional PCR and sequencing of the gltA, ompA, ompB, 17 kDa, and groEL genes. Six (11.3%) Amblyomma longirostre and Amblyomma geayi ticks collected from passeriform birds, yielded amplicons of the expected size. Amplicons were sequenced, and BLAST results collectively showed that all sequences had 99-100% nucleotide identity with Rickettsia amblyommatis (formerly, 'Candidatus Rickettsia amblyommii'). Three different R. amblyommatis strains were identified. Four new tick species-host associations and the first detection of R. amblyommatis in A. geayi in Costa Rica are also reported.

Molecular Detection of Plasmodium malariae/Plasmodium brasilianum in Non-Human Primates in Captivity in Costa Rica.

One hundred and fifty-two blood samples of non-human primates of thirteen rescue centers in Costa Rica were analyzed to determine the presence of species of Plasmodium using thick blood smears, semi-nested multiplex polymerase chain reaction (SnM-PCR) for species differentiation, cloning and sequencing for confirmation. Using thick blood smears, two samples were determined to contain the Plasmodium malariae parasite, with SnM-PCR, a total of five (3.3%) samples were positive to P. malariae, cloning and sequencing confirmed both smear samples as P. malariae. One sample amplified a larger and conserved region of 18S rDNA for the genus Plasmodium and sequencing confirmed the results obtained microscopically and through SnM-PCR tests. Sequencing and construction of a phylogenetic tree of this sample revealed that the P. malariae/P. brasilianum parasite (GenBank KU999995) found in a howler monkey (Alouatta palliata) is identical to that recently reported in humans in Costa Rica. The SnM-PCR detected P. malariae/P. brasilianum parasite in different non-human primate species in captivity and in various regions of the southern Atlantic and Pacific coast of Costa Rica. The similarity of the sequences of parasites found in humans and a monkey suggests that monkeys may be acting as reservoirs of P.malariae/P. brasilianum, for which reason it is important, to include them in control and eradication programs.

Morphological and molecular characterization of the metacercaria of Paragonimus caliensis, as a separate species from P. mexicanus in Costa Rica.

The trematode Paragonimus mexicanus is the etiological agent of paragonimiasis, a food-borne zoonotic disease in Latin America. This species, as well as Paragonimus caliensis, have been reported from Costa Rica, but it is not known if the two are synonymous. Two types of Paragonimus metacercariae from freshwater pseudothelphusid crabs from several localities in Costa Rica were recognized by light microscopy. Morphologically, these corresponded to descriptions of P. mexicanus and P. caliensis. Metacercariae of the former species lacked a membrane or cyst and their bodies were yellow in color. Those of P. caliensis were contained in a transparent thin cyst and were pink in color. Morphotypes of metacercariae were determined using scanning electron microscopy (SEM). Based on the number and distribution of papillae in the ventral sucker, three morphotypes were found for P. mexicanus and two for P. caliensis. Analysis of DNA sequences (nuclear ribosomal 28S and ITS2 genes, and partial mitochondrial cox1 gene) confirmed the presence of P. mexicanus and provided the first molecular data for P. caliensis. The two species are phylogenetically distinct from each other and distant from the Asian species. The confirmation of P. caliensis as a separate species from P. mexicanus raises several questions about the ecology, biological diversity, and epidemiology of the genus Paragonimus in Costa Rica.

[22q11.2DS Syndrome as a Genetic Subtype of Schizophrenia]. / El síndrome 22q11.2DS como un subtipo genético de esquizofrenia.

INTRODUCTION: The 22q11.2 deletion syndrome (22q11.2DS) is associated with the microdeletion of this chromosomal region, and represents the second most common genetic syndrome after Down's syndrome. In patients with schizophrenia, 22q11.2DS has a prevalence of 2%, and in selected groups can be increased to between 32-53%. OBJECTIVE: To describe the generalities of 22q11.2DS syndrome as a genetic subtype of schizophrenia, its clinical characteristics, molecular genetic aspects, and frequency in different populations. METHODS: A review was performed from 1967 to 2013 in scientific databases, compiling articles about 22q11.2DS syndrome and its association with schizophrenia. RESULTS: The 22q11.2 DS syndrome has a variable phenotype associated with other genetic syndromes, birth defects in many tissues and organs, and a high rate of psychiatric disorders, particularly schizophrenia. Likewise, it has been identified in clinical populations with schizophrenia selected by the presence of common syndromic characteristics. FISH, qPCR and MLPA techniques, and recently, aCGH and NGS technologies, are being used to diagnose this microdeletion. CONCLUSIONS: It is important in clinical practice to remember that people suffering the 22q11.2DS have a high genetic risk for developing schizophrenia, and it is considered that the simultaneous presence of this disease and 22q11.2DS represents a genetic subtype of schizophrenia. There are clear phenotypic criteria, molecular and cytogenetic methods to diagnose this group of patients, and to optimize a multidisciplinary approach in their monitoring.

El síndrome 22q11.2D S como un subtipo genético de esquizofrenia / 22q11.2 DS Syndrome as a Genetic Subtype of Schizophrenia

Introducción: El síndrome de deleción 22q11.2 (22q11.2 DS) se produce por microdeleciones del brazo largo del cromosoma 22 en la región q11.2. Después del síndrome de Down, es el segundo síndrome genético más común. En pacientes con esquizofrenia, el 22q11.2 DS tiene una prevalencia del 2%, mientras que en personas con esquizofrenia seleccionadas por características físicas específicas, aumenta un 32-53%. Objetivo: Describir las generalidades del 22q11.2 DS, sus características clínicas, los aspectos genético-moleculares y la frecuencia de la microdeleción de 22q11.2 en diferentes poblaciones. Métodos: Se hizo una revisión desde 1967 hasta 2013 en bases de datos de publicaciones científicas, orientada a recopilar artículos sobre el 22q11.2 DS y su relación con la esquizofrenia. Resultados: El 22q11.2 DS es una entidad genética que se asocia a un fenotipo variable relacionado con defectos congénitos en diferentes tejidos y órganos, así como a una alta frecuencia de trastornos psiquiátricos, particularmente la esquizofrenia. Se ha identificado alta prevalencia en grupos de personas con esquizofrenia seleccionadas por características sindrómicas comunes, como dificultades de aprendizaje, rasgos faciales típicos, anomalías palatales y defectos cardiacos congénitos. Las técnicas de FISH, qPCR, MLPA y, recientemente, aCGH y NGS se están usando para diagnosticar esta microdeleción. Conclusiones: En la práctica clínica es importante tener presente que las personas con 22q11.2 DS tienen alto riesgo de sufrir esquizofrenia, ya que la región 22q11.2 alberga genes candidatos relacionados con vulnerabilidad a esquizofrenia. Se considera que la concomitancia de esta enfermedad y 22q11.2 DS representa un subtipo genético de esquizofrenia. y métodos citogenéticos y moleculares para diagnosticar a este grupo de pacientes y optimizar un abordaje multidisciplinario en su seguimiento.

Introduction: The 22q11.2 deletion syndrome (22q11.2 DS) is associated with the microdeletion of this chromosomal region, and represents the second most common genetic syndrome after Down's syndrome. In patients with schizophrenia, 22q11.2 DS has a prevalence of 2%, and in selected groups can be increased to between 32-53%. Objective: To describe the generalities of 22q11.2 DS syndrome as a genetic subtype of schizophrenia, its clinical characteristics, molecular genetic aspects, and frequency in different populations. Methods: A review was performed from 1967 to 2013 in scientific databases, compiling articles about 22q11.2 DS syndrome and its association with schizophrenia. Results: The 22q11.2 DS syndrome has a variable phenotype associated with other genetic syndromes, birth defects in many tissues and organs, and a high rate of psychiatric disorders, particularly schizophrenia. Likewise, it has been identified in clinical populations with schizophrenia selected by the presence of common syndromic characteristics. FISH, qPCR and MLPA techniques, and recently, aCGH and NGS technologies, are being used to diagnose this microdeletion. Conclusions: It is important in clinical practice to remember that people suffering the 22q11.2 DS have a high genetic risk for developing schizophrenia, and it is considered that the simultaneous presence of this disease and 22q11.2 DS represents a genetic subtype of schizophrenia. There are clear phenotypic criteria, molecular and cytogenetic methods to diagnose this group of patients, and to optimize a multidisciplinary approach in their monitoring.

Descriptive study of 20 patients with schizophrenia in Boyacá, Colombia / Estudio descriptivo de una muestra de pacientes con esquizofrenia residentes en el departamento de Boyacá, Colombia

Schizophrenia is a multifactorial disease with high genetic heterogeneity and complex inheritance. In Boyacá, Colombia, we studied a group of 20 schizophrenic patients (16 men and 4 women) to establish their sociodemographic and clinical characteristics as well as their genetic and precipitating factors. The patients were analyzed using cytogenetic studies and a descriptive analysis of qualitative and quantitative variables. The disease frequently first manifested in young adults (average age of initiation: 22.5 years). The predominant subtype (8/20) was paranoid schizophrenia, and the onset was typically gradual (14/20). Precipitating factors were found in 15 patients: physical factors in nine patients, social factors in five patients and economic factor in one patient. All karyotypes were normal. Clinical features did not associate with either the sociodemographic characteristics or the genetic and predisposing factors, supporting the clinical heterogeneity of schizophrenia. Patients and their families received genetic counseling and explanations of the study's results, the possibility of recurrences and the risk of suffering the disease given an affected relative. Further and larger studies are required to determine if the factors evaluated in this study influence the development of the disease.

La esquizofrenia, enfermedad multifactorial, tiene gran heterogeneidad genética y herencia compleja. En Boyacá, Colombia, se estudió un grupo de 20 pacientes esquizofrénicos (16 hombres y cuatro mujeres) y se establecieron las características sociodemográficas y clínicas y los factores genéticos y precipitantes. Se hicieron estudio citogenético y un análisis descriptivo de las variables cualitativas y cuantitativas. Hubo predominio del comienzo de la enfermedad en adultos jóvenes (promedio de edad en el momento de la aparición: 22,5 años). Predominaron la esquizofrenia paranoide (8/20) con modo de aparición progresivo (14/20). Se hallaron factores precipitantes en 15 pacientes: físicos en nueve, sociales en cinco y económicos en uno. Todos los cariotipos fueron normales. Los rasgos clínicos no se asociaron con las características sociodemográficas ni con los factores genéticos y precipitantes, lo que evidencia gran heterogeneidad en las formas de manifestación de la enfermedad. Se dio asesoría genética a los pacientes y sus familias y se les explicaron los resultados, el riesgo de recurrencias y el de padecer la enfermedad cuando se tiene un pariente afectado. Es necesario analizar una serie mayor de casos, para poder determinar si los factores evaluados influyen en el desarrollo de la enfermedad.

Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica.

Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ∼1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ∼580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.

Anormalidades cromosómicas y esquizofrenia / Cromosomal abnormalities and schizophrenia

Introducción: La esquizofrenia es un trastorno mental que afecta a la población mundial, con una prevalencia del 1% y con heredabilidad hasta del 80%. Se han postulado cuatro enfoques para identificar genes de susceptibilidad y establecer marcadores moleculares asociados con la enfermedad: estudios de ligamiento genético, convergencia genómica, asociación y anormalidades cromosómicas. Objetivo: Mostrar anormalidades cromosómicas reportadas en pacientes con esquizofrenia como parte de los factores genéticos hallados en esta patología. Método: Se hizo una selección estratégica de 68 artículos publicados desde 1954 hasta 2008 en bases de datos científicas, clasificando las alteraciones de tipo numérico, estructural y mosaicos, tomando en cuenta sus contribuciones al estudio y relevancia. Resultados: Los cromosomas principalmente involucrados fueron, en orden de mayor a menor frecuencia, 18, 9, 11, 1, X, 22 y 21. En cuanto al tipo de anormalidad se encontraron alteraciones estructurales, mosaicismos, numéricas y también polimorfismos. Las anormalidades estructurales principalmente fueron translocaciones recíprocas balanceadas. Conclusiones: Con esta revisión no solamente se logró un acercamiento hacia la estimación de la frecuencia de estos hallazgos, sino tener un referente del tipo y frecuencia de estas alteraciones para evaluar el factor genético de la esquizofrenia, encaminados a comprender su patogenia...

Introduction: Schizophrenia is a mental disorder that affects the world population with a prevalence of 1% and a hereditability of up to 80% . Four approaches have been postulated to identify susceptibility genes and to establish molecular markers associated with the disease: Genetic linkage studies, genomic convergence, association, and chromosomal abnormalities. Objective: To show chromosomal abnormalities reported in patients with schizophrenia as part of the genetic factors found in this condition. Methods: A strategic selection was made of 68 articles published from 1954 to 2008 in scientific databases, and numerical, structural and mosaical type alterations were classified, taking into account their contributions to the study and relevance. Results: The mainly involved chromosomes were, in order of frequency, 18, 9, 11, 1, X, 22 and 21. As to type of abnormalities, structural and numerical alterations were found, as well as mosaicims and polymorphisms. Conclusions: With this review not only the calculation of the frequency of these findings was made more possible, but it also provided a reference of the type and frequency of these alterations to evaluate the genetic factor of schizophrenia, aimed at understanding its pathogeny...

Vozes no silêncio: um grupo de formação crítico-reflexiva de professoras de alunos com autismo / Voices of silence: a critical-reflexive education for treachers of autistic students

Esta pesquisa teve como objetivo analisar um processo de formação que procurou desenvolver, em professoras de alunos com transtornos autistas, uma postura críticoreflexiva, através da apropriação de conhecimentos e competências de atuação voltados para as características desse aluno. Adotamos as pesquisas ação e participante como métodos investigativos e a coleta de dados foi descritiva. Utilizamos as fundamentações teóricas de Donald A. Schön, Pimenta e Contreras sobre o uso da reflexão nos grupos de formação de profissionais, que foram articuladas com informações sobre as novas políticas de inclusão no ensino público, ressaltando a importância de um espaço que fosse propício à aprendizagem, valorizasse a interação entre os professores, respeitasse os tempos individuais e as formas diversas de aprender. Concluímos acreditando que foram obtidos indicadores de êxito nessa abordagem crítico-reflexiva de formação de professores, através de depoimentos e avaliações das participantes que expressaram o seu crescimento profissional e pessoal e suas possibilidades de reflexão sobre a prática como professoras de alunos com necessidades educativas especiais, levando-nos a refletir o quanto podemos caminhar para chegarmos mais perto de uma educação que seja realmente um direito de todo e qualquer cidadão

Vozes no silêncio: um grupo de formação crítico-reflexiva de professoras de alunos com autismo / Voices of silence: a critical-reflexive education for treachers of autistic students / Voces en el silencio: un equipo de formación crítico reflexiva de profesores de alumnos con autismo

Esta pesquisa teve como objetivo analisar um processo de formação que procurou desenvolver, em professoras de alunos com transtornos autistas, uma postura críticoreflexiva, através da apropriação de conhecimentos e competências de atuação voltados para as características desse aluno. Adotamos as pesquisas ação e participante como métodos investigativos e a coleta de dados foi descritiva. Utilizamos as fundamentações teóricas de Donald A. Schön, Pimenta e Contreras sobre o uso da reflexão nos grupos de formação de profissionais, que foram articuladas com informações sobre as novas políticas de inclusão no ensino público, ressaltando a importância de um espaço que fosse propício à aprendizagem, valorizasse a interação entre os professores, respeitasse os tempos individuais e as formas diversas de aprender. Concluímos acreditando que foram obtidos indicadores de êxito nessa abordagem crítico-reflexiva de formação de professores, através de depoimentos e avaliações das participantes que expressaram o seu crescimento profissional e pessoal e suas possibilidades de reflexão sobre a prática como professoras de alunos com necessidades educativas especiais, levando-nos a refletir o quanto podemos caminhar para chegarmos mais perto de uma educação que seja realmente um direito de todo e qualquer cidadão.

The main objective of this research was the analysis of a formation process for teachers of autistic students intended to bring to these professionals a reflective-critic position through the appropriation of knowledges and competencies targeted to the characteristics of these special-needs students. The action-research and participant observation procedures were the methodological approaches used and the data collection was done in a descriptive fashion. We have adopted Donald A. Schön, Pimenta and Contreras theories about the use of reflection in the formation professional groups, which were articulated with information about the new inclusion policies on public education. Our conclusion is based on what we believe are success indicators of this reflective-critic teachers formation approach. The participants expressed their personal and professional evolution process and the possibilities of reflection about their behavior as teachers of students deserving special educational needs, leading us wondering how much we can step further into education as a fundamental right for each and every citizen.

Esta investigación tuvo como objetivo analisar un proceso de formación que buscó desarrollar en profesoras de alumnos con trastornos autistas, una postura crítca reflexiva, a través de la apropiación de conocimientos y competencias de actuación volcadas a las características de este tipo de alumno. Como método adoptamos la investigación acción participante y la selección de datos con carácter descriptivo. Utilizamos las fundamentaciones teóricas de Donal A. Schön, Pimenta y Contreras sobre el uso de la reflexión en los grupos de formación de profesional, que se articularon con informaciones sobre las nuevas políticas de inclusión en la enseñanza pública, resaltando la importancia de un espacio que propicie el aprendizaje, valorice la interacción entre los profesores y respete los tiempos individuales y las formas distintas de aprender.

[Development of an optimized formulation of damask marmalade with low energy level using Taguchi methodology]. / Desarrollo de una formulación optimizada de mermelada de damasco de bajo contenido calórico utilizando la metodología Taguchi.

The goal of this present study was the development of an optimized formula of damask marmalade low in calories applying Taguchi methodology to improve the quality of this product. The selection of this methodology lies on the fact that in real life conditions the result of an experiment frequently depends on the influence of several variables, therefore, one expedite way to solve this problem is utilizing factorial desings. The influence of acid, thickener, sweetener and aroma additives, as well as time of cooking, and possible interactions among some of them, were studied trying to get the best combination of these factors to optimize the sensorial quality of an experimental formulation of dietetic damask marmalade. An orthogonal array L8 (2(7)) was applied in this experience, as well as level average analysis was carried out according Taguchi methodology to determine the suitable working levels of the design factors previously choiced, to achieve a desirable product quality. A sensory trained panel was utilized to analyze the marmalade samples using a composite scoring test with a descriptive acuantitative scale ranging from 1 = Bad, 5 = Good. It was demonstrated that the design factors sugar/aspartame, pectin and damask aroma had a significant effect (p < 0.05) on the sensory quality of the marmalade with 82% of contribution on the response. The optimal combination result to be: citric acid 0.2%; pectin 1%; 30 g sugar/16 mg aspartame/100 g, damask aroma 0.5 ml/100 g, time of cooking 5 minutes. Regarding chemical composition, the most important results turned out to be the decrease in carbohydrate content compaired with traditional marmalade with a reduction of 56% in coloric value and also the amount of dietary fiber greater than similar commercial products. Assays of storage stability were carried out on marmalade samples submitted to different temperatures held in plastic bags of different density. Non percetible sensorial, microbiological and chemical changes were detected after 90 days of storage under controlled conditions.

Pressupostos epistemologicos para a pesquisa em ensino de ciencias

Tendo como referencia a teoria piagetiana de equilibracao, este artigo examina aspectos da construcao do conhecimento, em especial do conhecimento cientifico, retirando exempols de conteudos especificos de Ciencias. Discute a funcao do professor na construcao do conhecimento pelo aluno, buscando subsidiar a pesquisa em ensino em uma perspectiva construtivista.

O enfermeiro diante do uso de drogas vasoativas / The murse facing the use of vasoactive drugs

Neste trabalho, as autoras procuram demonstrar a importância do controle rigoroso do gotejamento de drogas vasoativas, mais especificamente em relaçäo àlopamina e nitroprussiato de sódio, por considerar em que essas drogas, pela proximidade de seus efeitos terapeuticos e colaterais, e pelos diferentes efeitos nas diferentes dosagens, podem vir a ser eficazes agentes de introgenia

Da malária à insuficiência renal aguda / Malaria and acute renal failure

Neste artigo tenta-se evidenciar a malária, através do relato de um caso, como uma doença que pode afetar gravemente a funçäo renal. Destacam-se também alguns pontos prioritários no tratamento de enfermagem.

Drogas ilícitas: prevenção do uso na região atendida pela UBS CAIC-Patos de Minas

INTRODUÇÃO: A droga consome a vida do usuário, e o usuário destrói a vida de todos a sua volta. A faixa etária de 10 a 15 anos é um período decisivo para o contato e permanência no mundo das drogas. Por essa razão é importante que voltemos nosso olhar, como unidade de saúde, para os riscos que a exposição às drogas pode causar. É muito importante que a atenção primária à saúde, porta de entrada do SUS, esteja engajada em buscar meios para diminuir a incidência de jovens usuários de drogas e em ajudar de forma eficaz aqueles que já estão sofrendo com isso. JUSTIFICATIVA: Por serem os principais alvos das drogas os adolescentes e crianças são também o alvo desta intervenção, que buscará diminuir a incidência de jovens no mundo das drogas por meio de conversas explicativas e de apoio para que os jovens tenham conhecimento sobre o mundo das drogas e que tenham também alternativas para não caírem nele. OBJETIVOS: Elaborar um projeto de intervenção para conscientizar adolescentes e crianças acima de 10 anos quanto aos riscos do uso de drogas na área de abrangência da unidade de saúde CAIC de Patos de Minas. METODOLOGIA:Serão desenvolvidas palestras sobre os riscos do uso de drogas e alternativas para não ter contato com o produto. O projeto contará com uma palestra semanal em cada turno da escola, por um período de 10 semanas. Os pais também serão convidados a participar. PROJETO DE INTERVENÇÃO: Serão realizadas palestras semanais por 10 semanas em umminicurso sobre o uso de drogas. Nestas palestras contaremos com uma equipe multiprofissional, com psicólogo, assistente social, educador físico, fisioterapeuta, nutricionista, além da equipe de saúde com médica, enfermeira, técnicos de enfermagem e agentes comunitários de saúde. Além destes profissionais contaremos com o depoimento de um ex-usuário de drogas que discorrerá sobre sua vida antes, durante e depois do uso de drogas. A cada 15 dias serão realizadas reuniões para reavaliação do projeto e para observar a repercussão do mesmo dentro da escola