RESUMEN
Paracellular permeability is mediated by the epithelial cell tight junction. Studies in intestinal and other epithelia have suggested that the activity of src family kinases (SFKs) increases epithelial paracellular permeability through its action on the tight junction protein, occludin, but the involvement of SFKs and occludin in regulation of renal epithelial paracellular permeability is unclear. In this study, the role of SFKs in regulation of renal epithelial paracellular permeability and the involvement of occludin protein in this regulatory event was examined in two renal epithelial cell lines, LLC-PK(1) (proximal tubule-like) and MDCK (distal tubule-like). The effect of broad spectrum SFK inhibitors on paracellular permeability of calcein and fluorescein-dextran3000 were examined. SFK inhibitor treatment increased paracellular movement of both compounds in both renal epithelial cell lines. The SFK inhibitor effect was concentration-dependent and, at low concentrations, was not associated with cell damage/death. Response to SFK inhibitors was acquired progressively after cell populations attained confluence suggesting maturation of the regulatory mechanism. Increased paracellular permeability was not associated with dramatic changes in total cell content of occludin protein, its partitioning between detergent-soluble and -insoluble fractions, or its subcellular localization. Further, the SFK-induced increase in paracellular permeability was unaffected by either occludin protein overexpression or occludin protein knockdown. These results demonstrate that SFK activity decreases paracellular permeability of renal epithelial cells, as opposed to its effect in intestinal epithelial cells, and that this regulation is not mediated by occludin protein.
Asunto(s)
Células Epiteliales/enzimología , Túbulos Renales/enzimología , Ocludina/metabolismo , Uniones Estrechas/enzimología , Familia-src Quinasas/metabolismo , Actinas/metabolismo , Animales , Dextranos/metabolismo , Perros , Relación Dosis-Respuesta a Droga , Células Epiteliales/efectos de los fármacos , Fluoresceínas/metabolismo , Túbulos Renales/citología , Túbulos Renales/efectos de los fármacos , Células LLC-PK1 , Células de Riñón Canino Madin Darby , Ocludina/genética , Permeabilidad , Inhibidores de Proteínas Quinasas/farmacología , Transporte de Proteínas , Porcinos , Uniones Estrechas/efectos de los fármacos , Factores de Tiempo , Transfección , Proteína de la Zonula Occludens-1/metabolismo , Proteína de la Zonula Occludens-2/metabolismo , Familia-src Quinasas/antagonistas & inhibidoresRESUMEN
Mutations in rumi result in a temperature-sensitive loss of Notch signaling in Drosophila. Drosophila Rumi is a soluble, endoplasmic reticulum-retained protein with a CAP10 domain that functions as a protein O-glucosyltransferase. In human and mouse genomes, three potential Rumi homologues exist: one with a high degree of identity to Drosophila Rumi (52%), and two others with lower degrees of identity but including a CAP10 domain (KDELC1 and KDELC2). Here we show that both mouse and human Rumi, but not KDELC1 or KDELC2, catalyze transfer of glucose from UDP-glucose to an EGF repeat from human factor VII. Similarly, human Rumi, but not KDELC1 or KDELC2, rescues the Notch phenotypes in Drosophila rumi clones. During characterization of the Rumi enzymes, we noted that, in addition to protein O-glucosyltransferase activity, both mammalian and Drosophila Rumi also showed significant protein O-xylosyltransferase activity. Rumi transfers Xyl or glucose to serine 52 in the O-glucose consensus sequence ( ) of factor VII EGF repeat. Surprisingly, the second serine (S53) facilitates transfer of Xyl, but not glucose, to the EGF repeat by Rumi. EGF16 of mouse Notch2, which has a diserine motif in the consensus sequence ( ), is also modified with either O-Xyl or O-glucose glycans in cells. Mutation of the second serine (S590A) causes a loss of O-Xyl but not O-glucose at this site. Altogether, our data establish dual substrate specificity for the glycosyltransferase Rumi and provide evidence that amino acid sequences of the recipient EGF repeat significantly influence which donor substrate (UDP-glucose or UDP-Xyl) is used.