Isolation and sequencing of Orthoflavivirus ilheusense from mosquitoes collected in the Brazilian cerrado, West-Central region, Brazil.

The Orthoflavivirus ilheusense (ILHV) is an arbovirus that was first isolated in Brazil in 1944 during an epidemiologic investigation of yellow fever. Is a member of the Flaviviridae family and it belongs to the antigenic complex of the Ntaya virus group. Psorophora ferox is the primary vector of ILHV and this study presents the isolation and phylogenetic analysis of ILHV in a pool of Ps. ferox collected in the state of Goiás in 2021. Viral isolation tests were performed on Vero cells and C6/36 clones. The indirect immunofluorescence test (IFI) was used to confirm the positivity of the sample. The positive sample underwent RT-qPCR, sequencing, and phylogenetic analysis. This is the first report of ILHV circulation in this municipality and presented close relationship between this isolate and another ILHV isolate collected in the city of Belém (PA).

Genomic epidemiology unveils the dynamics and spatial corridor behind the Yellow Fever virus outbreak in Southern Brazil.

Despite the considerable morbidity and mortality of yellow fever virus (YFV) infections in Brazil, our understanding of disease outbreaks is hampered by limited viral genomic data. Here, through a combination of phylogenetic and epidemiological models, we reconstructed the recent transmission history of YFV within different epidemic seasons in Brazil. A suitability index based on the highly domesticated Aedes aegypti was able to capture the seasonality of reported human infections. Spatial modeling revealed spatial hotspots with both past reporting and low vaccination coverage, which coincided with many of the largest urban centers in the Southeast. Phylodynamic analysis unraveled the circulation of three distinct lineages and provided proof of the directionality of a known spatial corridor that connects the endemic North with the extra-Amazonian basin. This study illustrates that genomics linked with eco-epidemiology can provide new insights into the landscape of YFV transmission, augmenting traditional approaches to infectious disease surveillance and control.

Back to Where It Was First Described: Vectors of Sylvatic Yellow Fever Transmission in the 2017 Outbreak in Espírito Santo, Brazil.

Evidence of sylvatic yellow fever was first reported in Atlantic Forest areas in Espírito Santo, Brazil, during a yellow fever virus (YFV) outbreak in 1931. An entomological survey was conducted in six forest sites during and after an outbreak reported ~80 years after the last case in the area. Among 10,658 mosquitoes of 78 species, Haemagogus leucocelaenus, and Hg. janthinomys/capricornii were considered the main vectors as they had a relatively high abundance, co-occurred in essentially all areas, and showed high YFV infection rates. Sabethes chloropterus, Sa. soperi, Sa. identicus, Aedes aureolineatus, and Shannoniana fluviatilis may have a secondary role in transmission. This is the first report of Sa. identicus, Ae. aureolineatus, and Sh. fluviatilis infected with YFV. Our study emphasizes the importance of entomological monitoring and maintenance of high vaccination coverage in receptive areas to YFV transmission.

Chikungunya virus ECSA lineage reintroduction in the northeasternmost region of Brazil.

The Northeast region of Brazil registered the second-highest incidence proportion of Chikungunya fever in 2019. In that year, an outbreak consisting of patients presenting with febrile disease associated with joint pain was reported by the public primary health care service in the city of Natal, in the state of Rio Grande do Norte, in March 2019. At first, the aetiological agent of the disease was undetermined. Since much is still unknown about chikungunya virus' (CHIKV) genomic diversity and evolutionary history in this northeasternmost state, we used a combination of portable whole-genome sequencing, molecular clock, and epidemiological analyses that revealed the reintroduction of the CHIKV East-Central-South-African (ECSA) lineage into Rio Grande do Norte. We estimated that the CHIKV ECSA lineage was first introduced into Rio Grande do Norte in early June 2014, while the 2019 outbreak clade diverged around April 2018, during a period of increased Chikungunya incidence in the Southeast region, which might have acted as a source of virus dispersion towards the Northeast region. Together, these results confirm that the ECSA lineage continues to spread across the country through interregional importation events, likely mediated by human mobility.

Fortalecimento da vigilância em saúde no Brasil: Rede de Monitoramento da Resistência dos Triatomíneos aos Inseticidas / Strengthening of health surveillance in Brazil: Monitoring network of the insecticide resistance in Triatominae

Em 2010 foi criada no Brasil, pelo Ministério da Saúde e pelo Laboratório de Referência em Triatomíneos e Epidemiologia da Doença de Chagas da Fundação Oswaldo Cruz a "Rede de Monitoramento da Resistência de Triatomíneos aos Inseticidas" com vistas a: 1) caracterizar e monitorar a suscetibilidade/resistência das populações triatomínicas brasileiras aos inseticidas, 2) desenvolver métodos destinados ao diagnóstico da resistência dos triatomíneos aos inseticidas e 3) contribuir na avaliação e melhoria das atividades desenvolvidas junto ao controle químico dos triatomíneos. A implantação desta iniciativa, que possui hoje reconhecimento internacional junto a Organização Mundial de Saúde, está apresentada neste trabalho de forma detalhada.

Epidemiological characteristics of yellow fever in Brazil, 2000-2012. / Características epidemiológicas da febre amarela no Brasil, 2000-2012.

OBJECTIVE: this study aims to describe the epidemiological characteristics of yellow fever in Brazil in the period 2000-2012. METHODS: this is a descriptive ecological epidemiological study, using information from Ministry of Health databases. RESULTS: 326 cases of yellow fever were confirmed in Brazil during this period, with 156 deaths and an average case fatality rate of 47.8%; the young male adult age group was the most affected; in epizootic terms, 2,856 suspected cases of yellow fever in non-human primates were reported and 31.1% of these were confirmed by laboratory tests; during the study period the area in which sylvatic transmission of the disease occurs was found to have expanded to densely population regions, such as South, Southeast and Midwest Brazil. CONCLUSION: the risk of urban yellow fever transmission persists, as sylvatic incidence of the disease has expanded to regions with high Aedes aegypti infestation, this being the mosquito responsible for urban transmission of the disease.

Características epidemiológicas da febre amarela no Brasil, 2000-2012 / Características epidemiológicas de la fiebre amarilla en Brasil en el período 2000-2012 / Epidemiological characteristics of yellow fever in Brazil, 2000-2012

OBJETIVO: o estudo visa descrever as características epidemiológicas da febre amarela no Brasil no período de 2000 a 2012. MÉTODOS: estudo epidemiológico, ecológico, descritivo, utilizando informações dos bancos de dados do Ministério da Saúde. RESULTADOS: foram confirmados 326 casos de febre amarela no país nesse período, com 156 óbitos e taxa de letalidade média de 47,8%; o grupo de adultos jovens do sexo masculino foi o mais acometido; nas epizootias, foi identificado um total de 2.856 primatas não humanos notificados com suspeita de febre amarela, 31,1% deles confirmados laboratorialmente; no período estudado, foi identificada expansão da área de transmissão silvestre da doença para regiões densamente povoadas, como Sul, Sudeste e Centro-Oeste. CONCLUSÃO: persiste o risco de transmissão urbana da febre amarela, pois a incidência silvestre da doença tem se expandido para regiões onde existe alta infestação do Aedes aegypti, mosquito transmissor do ciclo urbano da doença.

OBJETIVO: el estudio tiene como objetivo describir las características epidemiológicas de la fiebre amarilla en Brasil entre 2000 y 2012. MÉTODOS: estudio epidemiológico descriptivo, ecológico, utilizando información de bases de datos del Ministerio de Salud. RESULTADOS: se confirmaron 326 casos de fiebre amarilla en el país en este periodo, con un total de 156 muertes y una tasa de letalidad de 47,8%; el grupo de adultos jóvenes del sexo masculino fue el más afectado; se identificó un total de 2.856 primates notificados sospechosos de fiebre amarilla, de los cuales 31,1% fueron confirmados laboratoriálmente; en el período estudiado, identificamos una expansión del área de transmisión silvestre de la enfermedad a zonas densamente pobladas como el Sur, Sudeste y Centro-Oeste. CONCLUSIÓN: persiste el riesgo de transmisión de fiebre amarilla, ya que la incidencia de la enfermedad se ha extendido a regiones donde hay una alta infestación de Aedes aegypti, el mosquito transmisor del ciclo urbano de la enfermedad.

OBJECTIVE: this study aims to describe the epidemiological characteristics of yellow fever in Brazil in the period 2000-2012. METHODS: this is a descriptive ecological epidemiological study, using information from Ministry of Health databases. RESULTS: 326 cases of yellow fever were confirmed in Brazil during this period, with 156 deaths and an average case fatality rate of 47.8%; the young male adult age group was the most affected; in epizootic terms, 2,856 suspected cases of yellow fever in non-human primates were reported and 31.1% of these were confirmed by laboratory tests; during the study period the area in which sylvatic transmission of the disease occurs was found to have expanded to densely population regions, such as South, Southeast and Midwest Brazil. CONCLUSION: the risk of urban yellow fever transmission persists, as sylvatic incidence of the disease has expanded to regions with high Aedes aegypti infestation, this being the mosquito responsible for urban transmission of the disease.

Estado da arte da brucelose em humanos / The current state of brucellosis in humans

A brucelose, doença infecto-contagiosa causada por bactérias do gênero Brucella, tem chamado a atenção dos sistemas de saúde em todo o mundo por provocar enfermidades de caráter ocupacional e por ser considerada pela Organização Mundial da Saúde como doença emergente e agente potencial para bioterrorismo. O Brasil ainda não possui uma rede estruturada na saúde pública para diagnóstico da brucelose em humanos. O presente trabalho objetiva realizar uma atualização sobre os principais aspectos que envolvem esta doença. Para este fim, uma revisão bibliográfica sistematizada foi realizada. Foram revisados aspectos etiológicos, clínicos, epidemiológicos e diagnósticos, dentre outros, com o intuito de atualizar os profissionais e pesquisadores da área. Encontraram-se 31 artigos, dos quais 28 foram utilizados. Constata-se, pela revisão, que muitos dos aspectos da patogenia, profilaxia, diagnóstico e terapia da doença ainda não estão completamente compreendidos, porém progresso substancial tem sido alcançado no entendimento da base molecular da genética da Brucella e da patogênese da infecção. Conclui-se afirmando a importância da padronização diagnóstica da doença, pois é com base nos dados fornecidos pelo diagnóstico que se poderá instituir medidas de vigilância.

Brucellosis, an infectious disease caused by bacteria of the genus Brucella, has drawn the attention of healthcare providers worldwide because it causes occupational diseases and is regarded as an emerging disease and a potential agent for bioterrorism by the World Health Organization. Brazil does not have a structured public health network for brucellosis diagnosis in humans. This work aims to provide an update on its main aspects of this disease. A systematic literature review was conducted for this purpose. Etiological, clinical, epidemiological and diagnostic aspects were reviewed, among others, in order to update professionals and researchers in the field. A total of 31 articles were found, of which 28 were used. This review shows that many aspects of pathogenesis, prevention, diagnosis and therapy of the disease are not yet fully understood, but substantial progress has been made in understanding the molecular basis of Brucella genetics and the pathogenesis of infection. We conclude by stating the importance of standardizing the diagnostic procedures of the disease because surveillance measures are imposed based on data provided by these diagnoses.