Activities and vectors responsible for the biological pollution in the Taranto Seas (Mediterranean Sea, southern Italy): a review.

Biological pollution, caused by the negative impact of alien species, also known as non-indigenous species (NIS), is regarded as one of the greatest threat to marine ecosystems. The recent upsurge in the number and spread of these species drew attention to putative vectors such as shipping and shellfish importation for culture and consumption. The port of Taranto in Southern Italy is a hub for several vectors as it serves commercial and military shipping, fishing and recreational boating, in addition to shellfish importation. An analysis of anthropogenic activities and possible vectors in Taranto Seas was recently carried out within the framework of the RITMARE Project, involving local stakeholders. Different categories of stakeholders answered dedicated questionnaires with a high degree of reticence, and this highlighted a general lack of awareness of the problems associated with alien species. Consequently, there is a strong need to instil a truly ecological awareness among the general public and stakeholders.

Biotechnological potential of the seaweed Cladophora rupestris (Chlorophyta, Cladophorales) lipidic extract.

Recently, with the advent of modern technologies, various marine organisms including algae are being studied as sources of natural substances effective on classical microorganisms and able to also combat the new trend of acquired resistance in microbes. In the present study the antimicrobial activity of the lipidic extract of the green seaweed Cladophora rupestris collected in a Mediterranean area, in two sampling periods (January and April), was assayed. The chemical characterization of the lipidic fractions was performed by gas-chromatography and multinuclear and multidimensional NMR spectroscopy. In the lipidic extract of C. rupestris collected in January an antibacterial activity against Enterococcus sp., Streptococcus agalactiae and Vibrio cholerae non-O1 was recorded; by contrast, bacterial inhibition was measured on several Vibrio species only in April. The fatty acid profile of C. rupestris lipidic extract, analyzed by gas chromatography, resulted mainly composed of palmitic, myristic, oleic, α linolenic, palmitoleic and linoleic acids. Moreover, since α-linolenic acid was the predominant ω3 fatty acid in April, we suggest its involvement in the antibacterial activity observed in this month, taking also into account that pure α-linolenic acid resulted effective towards some vibrios strains. C. rupestris fatty acid profile revealed also an interesting composition in polyunsaturated fatty acids in both the considered periods with the ω6/ω3 ratio lower than 1, leading to conclude that this macroalga may be employed as a natural source of ω3. Finally, the (1)H NMR spectrum in CDCl3 of algal lipid fractions showed the characteristic signals of saturated (SAFAs) and unsaturated fatty acids (UFAs) as well as other metabolites and a marked difference in free fatty acids (FFAs) content for the two examined algal lipid fractions. It is noteworthy that C. rupestris lipidic extracts show, by NMR spectroscopy, the signal pattern of polyhydroxybutyrate, a natural biocompatible and biodegradable polymer. In conclusion, on account of its antimicrobial activity, nutritional value and bioplastic content, C. rupestris lipidic extract can be considered a promising source for future biotechnological applications.

The lipidic extract of the seaweed Gracilariopsis longissima (Rhodophyta, Gracilariales): a potential resource for biotechnological purposes?

In recent years seaweeds increasingly attracted interest in the search for new drugs and have been shown to be a primary source of bioactive natural products including antibiotics. In the present investigation the antimicrobial activity of Gracilariopsis longissima lipidic extract was assayed and its chemical characterization was carried out by means of advanced analytical techniques such as gas-chromatography and multinuclear and multidimensional NMR spectroscopy. G. longissima lipidic extract showed an antibacterial activity against several Vibrio species. These results are interesting considering both the resistance against antibiotics developed by vibrios and the need to control fish and shellfish diseases due to vibriosis. Analysis of fatty acid methyl esters performed by gas-chromatography showed that palmitic acid methyl ester (16:0) was the predominant saturated fatty acid (42%), while, among monounsaturated fatty acids, oleic acid methyl ester (18:1) prevailed (8.5%). Because the palmitic acid represents the main component of fatty acids we hypothesized its involvement in the antibacterial activity observed. However, a pure sample of palmitic acid did not show an antibacterial activity. The fatty acid profile of G. longissima revealed also an interesting composition in polyunsaturated fatty acids and in particular the ratio of ω-3 to ω-6 fatty acids was >1 thus suggesting that this macroalga may be used as a natural source of ω3. Moreover, the (1)H NMR spectrum in CDCl(3) of algal lipid fraction shows the characteristic signals of saturated and unsaturated fatty acids as well as other metabolites. Interestingly, in the lipid extract the presence of polyhydroxybutyrate, a linear biodegradable and biocompatible polyester, was clearly identified by NMR spectroscopy. In conclusion, the lipidic extract of G. longissima on account of its antimicrobial activity, nutritional value and content in biodegradable and biocompatible polyester represents an interesting potential biotechnological resource.

Estimation of dietary intake of pesticide residues by the Italian population during 1997.

In 1997 the Italian monitoring programme for pesticide residues in food comprised 7951 samples of 152 different foods for which a total of 275 pesticides were analysed. For 90 pesticides, the National Estimated Daily Intakes (NEDIs) were calculated by using the 90th percentile value and the food consumption data for the general Italian population. The calculated NEDIs were found to represent only a small portion of the respective Acceptable Daily Intake (ADI).

[National surveillance system for acute poisoning caused by pesticides]. / Piano di sorveglianza nazionale per la rilevazione delle intossicazioni acute da antiparassitari.

In the present paper is presented the surveillance system for pesticide-related illnesses proposed by the Istituto Superiore di Sanità, in collaboration with the Ministry of Health. Through the collaboration of local or regional poison control centres and local health departments with public hospitals and emergency departments, cases of acute pesticide-related illnesses among persons seeking health care will be registered according to standard procedures. Data collected locally will be transmitted to the Istituto Superiore di Sanità for registration and evaluation of the association between symptoms and type of exposure and for severity of illnesses related to pesticide exposure. These data will be analysed at regular intervals in order to estimate the magnitude of the problem of acute pesticide-related illnesses in Italy and to identify priority areas for implementing public health prevention and intervention programs.

[An epidemiological study on dental-periodontal pathology in a sample of the school-age population of the city of Naples. I. Periodontal pathology]. / Indagine epidemiologica sulla patologia dento-parodontale in un campione della popolazione scolastica della città di Napoli. Nota I. Patologia parodontale.

The paper reports the results of a study of the prevalence of periodontal diseases found in a sample group, aged between 6 and 18, resident in Naples. The structure of the survey, the clinical methods of collecting data and the statistical processing of data were carried out according to WHO directives regarding the study of oral pathologies.

[An epidemiological study on dental-periodontal pathology in a sample of the school-age population of the city of Naples. II. Prevention and periodontal therapy]. / Indagine epidemiologica sulla patologia dento-parodontale in un campione della popolazione scolastica della città di Napoli. Nota II. Prevenzione e terapia parodontale.

The need for periodontal prevention and therapy was quantified according to the prevention of pathologies within the framework of an epidemiological study carried out on a sample population aged between 6 and 18 years. Data were collected using the CPITN method (WHO).