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1.
Anim Reprod Sci ; 219: 106512, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32828398

RESUMEN

The aim of the study was to determine the luteolytic dose of cloprostenol administered directly into the corpus luteum (CL; intra-luteal treatment, ILT) in dairy cattle. Cows of two control groups were treated with 500 µg of cloprostenol (Estrumate®) intramuscularly (IM-500) or via ILT with 0.2 mL of physiological solution (ILT-0). Cows of four experimental groups were treated by ILT with cloprostenol in doses 5, 25, 50 and 100 µg (ILT-5, -25, -50 and -100 groups). Progesterone concentrations (P4) and size of CL were evaluated to assess luteolysis at 0, 0.5, 1, 2, 4, 8, 24 and 48 h or at 0, 24 and 48 h after ILT, respectively. Cows in the ILT-0 and -5 groups were unaffected by ILT. The P4 concentrations were less in cows of the IM-500, as well as ILT-25, -50 and -100 groups at 48 h subsequent to ILT. The size of the CL was less in cows of IM-500, as well as ILT-25, -50 and -100 groups at 48 h after ILT. There were P4 concentrations of about 1 ng/mL 48 h after ILT in cows of the IM-500, as well as ILT-50 and -100 groups. In conclusion, the cloprostenol dose of 50 µg administered intra-luteally is a luteolytic dose in cows.


Asunto(s)
Bovinos , Cloprostenol/administración & dosificación , Cuerpo Lúteo/efectos de los fármacos , Luteólisis/efectos de los fármacos , Animales , Cloprostenol/farmacología , Cuerpo Lúteo/citología , Cuerpo Lúteo/diagnóstico por imagen , Industria Lechera , Vías de Administración de Medicamentos , Sincronización del Estro/efectos de los fármacos , Sincronización del Estro/fisiología , Femenino , Lactancia/efectos de los fármacos , Lactancia/fisiología , Fase Luteínica/sangre , Fase Luteínica/efectos de los fármacos , Luteólisis/fisiología , Ovario/diagnóstico por imagen , Ovario/efectos de los fármacos , Progesterona/sangre , Resultado del Tratamiento , Ultrasonografía/métodos , Ultrasonografía/veterinaria
2.
Theriogenology ; 89: 41-46, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-28043369

RESUMEN

Acid-base balance is one of the most vigorously regulated variables of the body, including genital organs. Subacute ruminal acidosis is a common disturbance in dairy cows that disturbs several biochemical indices in the blood, cerebrospinal fluid, and urine. The possible negative effects of metabolic acidosis on the follicular fluid (FF) composition and, subsequently, on oocyte quality, are not fully elucidated. This study aimed to evaluate the changes in acid-base balance (ABB) in FF and blood during acute metabolic acidosis in dairy heifers. Ten Holstein heifers were stimulated with FSH in eight decreasing doses at 12-hour intervals (D0-D3). Acidosis was induced by oral administration of sucrose at 9 g/kg of body weight, dissolved in 10 L of warm tap water, at D3. Samples were collected from each cow at 0, 8, 12, 16, 24, 32, 40, and 48 hours after treatment. Samples of FF, obtained by transvaginal follicular aspiration, and peripheral blood were examined for ABB parameters: pH, pCO2, pO2, HCO3-, and base excess (BE). A significant decrease in pH, HCO3-, and BE values in the blood, as well as FF, occurred after sucrose treatment. The lowest pH values occurred in blood at 16 hours, and in FF at 24 hours, after treatment (7.30 ± 0.05 and 7.33 ± 0.05, respectively). The lowest HCO3- values in blood (18.75 ± 3.2 mmol/L) and FF (18.07 ± 2.84 mmol/L) occurred 24 hours after treatment, as did the lowest BE values (-6.61 ± 3.7 mmol/L and -7.53 ± 3.89 mmol/L, in blood and FF, respectively). Significant correlations for HCO3- (r = 0.928), BE (r = 0.946), pH (r = 0.889), and pCO2 (r = 0.522) existed between blood and FF samples. The results demonstrated that metabolic acute acidosis substantially influences the characteristics of both serum and FF.


Asunto(s)
Equilibrio Ácido-Base/fisiología , Desequilibrio Ácido-Base/veterinaria , Acidosis/veterinaria , Enfermedades de los Bovinos/sangre , Líquido Folicular/metabolismo , Desequilibrio Ácido-Base/metabolismo , Acidosis/sangre , Enfermedad Aguda , Animales , Análisis de los Gases de la Sangre/veterinaria , Bovinos , Femenino , Hormona Folículo Estimulante/farmacología , Líquido Folicular/química , Concentración de Iones de Hidrógeno , Folículo Ovárico/metabolismo , Sacarosa/farmacología
3.
Reprod Domest Anim ; 48(5): 790-4, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23600627

RESUMEN

Mycobacterium avium subspecies paratuberculosis (MAP), the causal agent of paratuberculosis, was detected by quantitative real-time IS900 PCR in the follicular fluid from the reproductive tracts of cows originating from one infected herd. As well as being detected in follicular fluid of cows shedding bacteria in their faeces, MAP was also detected in the follicular fluid of one apparently healthy, non-shedding individual cow. The finding of MAP in follicular fluid is unexpected and could contribute to the lower viability of embryos and resultant lower pregnancy rate. In addition to finding contaminated follicular fluid, vaginal and uterine flush fluids were determined to be positive for the presence of MAP in 75% and 56.3% of the time of the cattle currently shedding MAP in their faeces, respectively. The presence of MAP in different parts of the reproductive tract was seen in clinically as well as subclinically infected cows. These findings extend our currently scant and contradictory knowledge about the dissemination of MAP in the reproductive tract of female cattle.


Asunto(s)
Genitales Femeninos/microbiología , Mycobacterium avium subsp. paratuberculosis/aislamiento & purificación , Paratuberculosis/microbiología , Complicaciones Infecciosas del Embarazo/veterinaria , Animales , Bovinos , Heces/microbiología , Femenino , Embarazo
4.
Theriogenology ; 75(9): 1582-95, 2011 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-21411133

RESUMEN

Pre-implantation embryos derived by in vitro fertilization differ in their developmental potential from embryos obtained in vivo. In order to characterize changes in gene expression profiles caused by in vitro culture environment, we employed microarray constructed from bovine oocyte and preimplantation embryo-specific cDNAs (BlueChip, Université Laval, Québec). The analysis revealed changes in the level of 134 transcripts between in vitro derived (cultured in COOK BVC/BVB media) and in vivo derived 4-cell stage embryos and 97 transcripts were differentially expressed between 8-cell stage in vitro and in vivo embryos. The expression profiles of 7 selected transcripts (BUB3, CUL1, FBL, NOLC1, PCAF, GABPA and CNOT4) were studied in detail. We have identified a switch from Cullin 1-like transcript variant 1 to Cullin 1 transcript variant 3 (UniGene IDs BT.36789 and BT.6490, respectively) expressions around the time of bovine major gene activation (8-cell stage). New fibrillarin protein was detected by immunofluorescence already in early 8-cell stage and this detection correlated with increased level of fibrillarin mRNA. The qRT-PCR analysis revealed significant differences in the level of BUB3, NOLC1, PCAF, GABPA and CNOT4 gene transcripts between in vivo derived (IVD) and in vitro produced (IVP) embryos in late 8-cell stage. The combination of these genes represents a suitable tool for addressing questions concerning normal IVD embryo development and can be potentially useful as a marker of embryo quality in future attempts to optimize in vitro culture conditions.


Asunto(s)
Blastocisto/metabolismo , Proteínas Cullin/genética , Desarrollo Embrionario/genética , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Animales , Secuencia de Bases , Bovinos , Proteínas Cullin/metabolismo , Medios de Cultivo , Técnicas de Cultivo de Embriones/veterinaria , Datos de Secuencia Molecular , Alineación de Secuencia
5.
Cesk Patol ; 44(3): 81-3, 2008 Jul.
Artículo en Checo | MEDLINE | ID: mdl-18783141

RESUMEN

Juxtaglomerular cell tumor (JGCT) is an uncommon tumor of the kidney, typically found in young adults. Patients with this tumor suffer from hypertension, hyperaldosteronism and hypokalaemia. Expression of renin and intracytoplasmatic rhomboid crystals or granules in electron microscopic picture are diagnostic features of this tumor. CD34 and CD117 immunoreactivity have recently been reported as helpful markers of JGCT.


Asunto(s)
Antígenos CD34/análisis , Aparato Yuxtaglomerular , Neoplasias Renales/patología , Proteínas Proto-Oncogénicas c-kit/análisis , Adolescente , Femenino , Humanos , Hipertensión Renal/etiología , Neoplasias Renales/química , Neoplasias Renales/complicaciones
6.
Anim Reprod Sci ; 96(1-2): 186-95, 2006 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-16448792

RESUMEN

The vitality of bovine oocytes stored in isolated follicles was examined. The aim of this work was to prolong the time of in vitro manipulation of oocytes before their maturation and develop a new alternative of oocyte "capacitation" to improve the quality of in vitro produced embryos. Follicles were dissected from the ovaries of slaughtered cows; subsequently, follicles were divided according to their diameter into three categories (2-3, 3-4 and 4-6 mm), and stored at 17-18 degrees C for 24 or 48 h in a modified tissue culture medium-199 (TCM-199) with reduced pH. After that time, the cumulus-oocyte complexes (COCs) were isolated, matured, fertilized, and embryos cultured in vitro for a total of 9 days. The percentage of total blastocysts, and hatched blastocysts developed from oocytes, initially kept ("capacitated") for 24h at 17-18 degrees C, within follicles of 3-6mm size categories, were significantly higher than that oocytes of the control [of control oocytes] (44.9 and 30.3% versus 36.2 and 20.4%, respectively). The oocytes of follicles stored for 48 h at 17-18 degrees C already had decreased developmental capacity. Interesting data were obtained when COCs of the 3-4 and 4-6 categories were additionally divided into two subgroups according to their presumed developmental history (originating from the supposed growing "fit" in contrast to the supposed regressing "unfit" follicles). The higher improvement in the rate of hatched blastocysts from 24h stored oocytes was observed only in the subgroup originated from "fit" COCs (15.3 versus 25.0%, and 20.0 versus 34.4%, in the 3-4 and 4-6mm categories, respectively). The transfer of 26 blastocysts (developed of follicles kept for 24h at 17-18 degrees C) to 26 recipient heifers resulted in 18 pregnancies. Storage of follicles at 17-18 degrees C in vitro resulted not only in recovery of higher numbers of blastocysts of better quality but also facilitated the safe transport of follicles for a long distance. The extended, time of follicle storage before the proper oocyte maturation allowed also the synchronization of an appropriate number of recipient animals according to the number of isolated follicles.


Asunto(s)
Bovinos , Fertilización In Vitro/veterinaria , Oocitos/fisiología , Folículo Ovárico/fisiología , Conservación de Tejido/veterinaria , Animales , Blastocisto/fisiología , Técnicas de Cultivo de Embriones/veterinaria , Transferencia de Embrión/veterinaria , Embrión de Mamíferos/fisiología , Femenino , Concentración de Iones de Hidrógeno , Folículo Ovárico/citología , Conservación de Tejido/métodos
7.
J Exp Biol ; 186(1): 145-56, 1994 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-9317518

RESUMEN

A micro-thermistor probe was inserted into the buccal cavity of freely swimming paddlefish to measure flow velocity during ram ventilation, ram suspension feeding and prey processing. Swimming speed was measured from videotapes recorded simultaneously with the buccal flow velocity measurements. Both swimming velocity and buccal flow velocity were significantly higher during suspension feeding than during ram ventilation. As the paddlefish shifted from ventilation to feeding, buccal flow velocity increased to approximately 60 % of the swimming velocity. During prey processing, buccal flow velocity was significantly higher than the swimming velocity, indicating that prey processing involves the generation of suction. The Reynolds number (Re) for flow at the level of the paddlefish gill rakers during feeding is about 30, an order of magnitude lower than the Re calculated previously for pump suspension-feeding blackfish. These data, combined with data available from the literature, indicate that the gill rakers of ram suspension-feeding teleost fishes may operate at a substantially lower Re than the rakers of pump suspension feeders.

8.
Acta Histochem ; 91(1): 43-9, 1991.
Artículo en Alemán | MEDLINE | ID: mdl-1666243

RESUMEN

The paper summarized some data about glycogen distribution in the tubal epithelium of healthy women aged 22 to 35 during the menstrual cycle at the ultrastructural level. For visualizing polysaccharide the periodic acid-thiosemicarbazide-silver proteinate reaction according to Thiéry (1967) was used. A high content of glycogen was characteristic of the ciliated cells in which 2 main distribution patterns were observed: the diffuse one and the depositary one. Glycogen particles or clusters also penetrated some of basal bodies (kinetosomes) as well as apical protrusions of these cells, in which cilia in different stages of death are visible. As for polysaccharide distribution in the nonciliated cells, it could be shown that they especially contained the monoparticulate glycogen. The ultrastructural distribution pattern of glycogen in the tubal epithelium of young women seems to be relative independent on phases of the menstrual cycle.


Asunto(s)
Glucógeno/análisis , Útero/química , Adulto , Epitelio/química , Epitelio/ultraestructura , Femenino , Humanos , Ciclo Menstrual , Ácido Peryódico , Semicarbacidas , Tinción con Nitrato de Plata , Útero/ultraestructura
9.
Acta Histochem ; 88(1): 65-9, 1990.
Artículo en Alemán | MEDLINE | ID: mdl-2162618

RESUMEN

2 histochemical methods were used to analyse the carbohydrates in the human oviductal epithelium, namely the periodic acid-semicarbazide-silver proteinate and the 'mild' periodic acid-thiosemicarbazide-silver proteinate technique. It was possible to detect periodate reactive substances and sialic acid-containing compounds, namely separately. The 'mild' periodic acid oxidation is a selective technique for the histochemical demonstration of the existence of unsubstituted acylneuraminic acids. The ultrahistochemical results show that, firstly, periodate reactive particles are located in the cytoplasm of ciliated and nonciliated cells. The material appears in form of beta-granula (glycogen). Secondly, we could observe fine granular reactive products in the lysosomal-like and lipid droplet-like structures. In these subjects the positive granula encircling ring-like the homogeneous matrix occur in the peripheral zone of the morphological substrate investigated. By the way, these granula situated in the periphery of organelles are alone reacting after staining with 'mild' periodic acid-thiosemicarbazide-silver proteinate; this means, the glycogen particles are unstained. Thus the findings indicate that, in the cells of the human oviductal epithelium, sialic acid-containing compounds are ultrahistochemically visualized by 'mild' periodic acid oxidation in combination with thiosemicarbazide and silver proteinate.


Asunto(s)
Trompas Uterinas/ultraestructura , Ácidos Siálicos/análisis , Cilios/ultraestructura , Epitelio/ultraestructura , Femenino , Humanos , Indicadores y Reactivos , Microscopía Electrónica , Ácido Peryódico
10.
Acta Histochem ; 88(2): 199-201, 1990.
Artículo en Inglés | MEDLINE | ID: mdl-1699382

RESUMEN

Using the mPA-TSC-SP staining procedure, it is possible to detect dense silver deposits in the marginal area of some morphological structures of the human oviductal epithelium. By reason of their pale grey homogeneous appearance, we suppose that they are lipid droplets. They probably contain sialoglycolipids lying at the rim of the droplets. The functional significance of the compounds is hitherto unknown.


Asunto(s)
Trompas Uterinas/ultraestructura , Lípidos/análisis , Ácidos Siálicos/análisis , Epitelio/ultraestructura , Femenino , Humanos , Ácidos Neuramínicos/análisis , Plata , Coloración y Etiquetado
11.
Acta Histochem ; 87(2): 137-9, 1989.
Artículo en Alemán | MEDLINE | ID: mdl-2516679

RESUMEN

The ultrahistochemical findings show that, in the oviductal epithelium of the ageing woman, glycogen particles are detectable in the basal bodies of the ciliated cells. The polysaccharide could be considered to be a reserve of energy in the ciliary action.


Asunto(s)
Trompas Uterinas/ultraestructura , Glucógeno/análisis , Adulto , Epitelio/ultraestructura , Femenino , Humanos , Persona de Mediana Edad
12.
Acta Histochem ; 86(2): 143-50, 1989.
Artículo en Inglés | MEDLINE | ID: mdl-2514548

RESUMEN

Ultrahistochemical method according to Thiéry (1967) was used to determine the occurrence and localization of glycogen in blastocysts developed in vivo and in blastocysts developed from 2-cell embryos of the mouse for 62 to 64 h in in vitro culture. The presence of glycogen was found in blastocysts of both experimental groups. Glycogen had a monoparticulate character, i.e. the form of beta-granules, localized above all in the ground cytoplasm of cells. Their size varied from 10 to 30 nm. In the blastocysts developed in the physiological uterine environment the glycogen content was relatively low, trophoblasts cells containing regularly a higher amount of glycogen particles than embryoblast cells. In the blastocysts developed in the culture medium in the presence of currently used energy sources the distribution and content of glycogen were clearly graded according to the cell types. Compared with the in vivo-blastocysts, an abnormally high amount of glycogen was observed in the cytoplasm of trophoblast cells, a medium amount in the prospective endoderm cells and the minimum amount in the prospective ectoderm cells. The authors are of the opinion that differences in the accumulation of glycogen and its occurrence in the individual cells are in connection with their position in the blastocyst and with their relation to the surrounding microenvironment. It can be judged from the findings of glycogen deposits inside autophagic vacuoles and multivesicular bodies as well as inside extracellular located sacs that simultaneously with glycogen accumulation there also proceeds its partial degradation in lysosomal structures of blastocyst cells.


Asunto(s)
Blastocisto/ultraestructura , Glucógeno/análisis , Animales , Blastocisto/fisiología , Núcleo Celular/ultraestructura , Femenino , Ratones , Microscopía Electrónica , Vacuolas/ultraestructura , Zona Pelúcida/ultraestructura
13.
Acta Histochem ; 86(2): 159-66, 1989.
Artículo en Alemán | MEDLINE | ID: mdl-2514549

RESUMEN

The Picrosirius-polarization method was applied to the selective morphological collagen detection in the human uterine tube. Solaminrot 4B (VEB Chemiekombinat Bitterfeld) was used as dyestuff which enhances the normal birefringency of collagen fibrous material in tissue sections. The results show that the connective tissue of the lamina propria mucosae consists of collagen fibres, namely considerably. The mucosal folds of uterine tubes of younger individuals contain delicate, weaker birefringent fibres stained yellowish-red and red-orange. The folds of oviducts of ageing individuals composed of compact, strong birefringent collagen fibrous material stained intensely red, red-orange, and yellow. This is characteristic of organs in the pre- and postmenopausal periods and in the senium. The findings suggest that the fibrous structures detected selectively in the oviductal mucosa by combinative method of Picrosolamin-staining and polarization microscopy are distinct collagen fibres. The delicate, weaker birefringent fibres could correspond with collagen type III and the thicker, stronger birefringent ones could represent collagen type I.


Asunto(s)
Colágeno/análisis , Trompas Uterinas/citología , Adulto , Compuestos Azo , Colorantes , Trompas Uterinas/ultraestructura , Femenino , Humanos , Microscopía Electrónica , Estructura Molecular , Membrana Mucosa/citología , Membrana Mucosa/ultraestructura
15.
Acta Histochem ; 80(2): 175-82, 1986.
Artículo en Alemán | MEDLINE | ID: mdl-3101372

RESUMEN

The ultrahistochemical findings show that, in the oviductal epithelium of the ageing woman, periodate reactive substances are detectable in ciliated and nonciliated cells. These periodate reactive particles have different intracellular localization and distribution patterns. Besides, cells are found in the epithelium, which either contain only few periodate reactive material or react negatively. These cells appear singularly or in groups.


Asunto(s)
Envejecimiento/fisiología , Histocitoquímica , Oviductos/citología , Reacción del Ácido Peryódico de Schiff , Adulto , Animales , Epitelio/análisis , Femenino , Humanos , Persona de Mediana Edad
16.
Vet Med (Praha) ; 30(10): 585-94, 1985 Oct.
Artículo en Checo | MEDLINE | ID: mdl-3933162

RESUMEN

In dairy cows of the Black Pied Lowland, Bohemian Pied breeds and their crossbreds loosely in large cow houses VKK 900 on slatted floors, 24% incidence of dermatitis digitalis was recorded during the period of one year. Relations between the occurrence of this disease, age and efficiency of dairy cows, time of parturition and season of the year were studied. Therapeutical effects of several methods of treatment were evaluated and compared. No effect of age and efficiency of dairy cows, nor of the year season on the occurrence of this disease was observed. A significantly higher occurrence was proved in the period before and after parturition, when 80% of the total occurrence of digitalis dermatitis were diagnosed. During this period, however, the cows were housed in a stable with markedly worse environmental circumstance than those in production stable. 91% cases of dermatitis were diagnosed on the digits of pelvic limbs. Relapses were determined only in five dairy cows. There was no case of the disease occurring in calves reared in the prophylactorium of the calf house. Therapeutical results were best after repeated mass treatment of the digits of dairy cows in 5% formaldehyde baths. The results of this study point at a conclusion that probable pathogens of this disease are specific infection agents, or that there are more synergic pathogens. A significant pre-disposition factor are bad environmental circumstances.


Asunto(s)
Enfermedades de los Bovinos/terapia , Dermatosis del Pie/veterinaria , Animales , Bovinos , Enfermedades de los Bovinos/epidemiología , Femenino , Dermatosis del Pie/epidemiología , Dermatosis del Pie/terapia , Pezuñas y Garras
18.
Cell Tissue Res ; 230(3): 661-70, 1983.
Artículo en Inglés | MEDLINE | ID: mdl-6850786

RESUMEN

During cleavage, both qualitative and quantitative morphologic characters of mouse ova change. Up to the 8-cell stage, the volume density of mitochondria remains nearly the same although it increases during early and late blastocyst stages. While a rise of the volume fraction of granular endoplasmic reticulum is noticed during cleavage, the volume density of agranular endoplasmic reticulum diminishes gradually from the 1-cell stage onwards. An increase in the volume fraction of autophagic vacuoles is found, the maximum being reached in early blastocysts. The volume fraction of crystalloid inclusions slightly increases after the 4-cell stage, but this increase is statistically insignificant. The volume density of filamentous material (plaques) conspicuously decreases in all studied embryos from the beginning of cleavage. Starting with the 2-cell stage, the volume fraction of lipid droplets remains practically unchanged. No differences in volume densities of the Golgi apparatus, multivesicular and residual bodies, and large cytoplasmic vesicles with medium electron-dense content are found between the respective cleavage stages.


Asunto(s)
Blastocisto/ultraestructura , Animales , Desarrollo Embrionario , Femenino , Ratones , Microscopía Electrónica , Óvulo/ultraestructura , Embarazo
19.
Z Mikrosk Anat Forsch ; 97(6): 915-28, 1983.
Artículo en Alemán | MEDLINE | ID: mdl-6670348

RESUMEN

Ultrastructural signs of atresia were studied in healthy women between 25 and 36 years of age. As a morphologic correlate of incipient atresia the dispersion of the paranuclear complex of organelles (Balbiani's yolk nucleus) and a significant affection of mitochondria were detected. The latter structures showed higher electron density of the matrix and fewer cristae, compared with normal oocytes. Dilation of endoplasmic reticulum tubules and vesicles and presence of numerous various-sized ooplasmic vacuoles were noted. Deep invaginations and dilation of the nuclear envelope were also observed in terminal phases of atresia. The atretic follicles were lined by dark and light granulosa cells arranged partly over each other. The character of their cytologic changes was similar to those described in the oocytes. In most atretic follicles there were found deposits of extracellular material, after ruthenium red staining reminding of the zona pellucida, between the oocyte and the granulosa cells, sometimes exclusively between the granulosa cells.


Asunto(s)
Folículo Ovárico/ultraestructura , Adulto , Femenino , Humanos , Microscopía Electrónica , Oocitos/ultraestructura , Organoides/ultraestructura , Folículo Ovárico/citología , Folículo Ovárico/fisiología
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