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1.
Oral Dis ; 28(7): 1947-1957, 2022 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-33740823

RESUMEN

OBJECTIVES: The present study was performed to compare the effectiveness of Ankaferd Blood Stopper® (ABS) with enamel matrix derivatives (EMD) for treating fenestration defects in rats. MATERIALS AND METHODS: Forty-eight male Wistar rats were randomly divided into six groups (each n = 8). Fenestration defects were created in all rats, to which ABS, EMD, or saline (S) was then applied. The rats were grouped and sacrificed at one of two different time points, as follows: ABS-10-group, ABS-treatment/sacrifice on day 10; EMD-10-group, EMD-treatment/sacrifice on day 10; S-10-group, S-treatment/sacrifice on day 10; ABS-38-group, ABS-treatment/sacrifice on day 38; EMD-38-group, EMD-treatment/sacrifice on day 38; and S-38-group, S-treatment/sacrifice on day 38. Then, histomorphometric analysis including measurements of new bone area (NBA) and new bone ratio (NBR), and immunohistochemical analysis including the determination of osteopontin (OPN) and type-III-collagen (C-III) expression were performed. RESULTS: The NBA and NBR were significantly higher in the ABS-10-group and EMD-10-group compared to the S-10-group (p < .05), and in the EMD-38-group compared to the S-38-group (p < .05). The levels of C-III and OPN immunoreactivity were significantly higher in the ABS-10-group compared to the S-10-group (p < .017). CONCLUSIONS: The results of this study suggested that ABS can promote early periodontal regeneration, although its efficacy seems to decrease over time.


Asunto(s)
Proteínas del Esmalte Dental , Animales , Proteínas del Esmalte Dental/farmacología , Proteínas del Esmalte Dental/uso terapéutico , Masculino , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Ratas , Ratas Wistar
2.
Clin Oral Investig ; 26(1): 659-671, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34251534

RESUMEN

OBJECTIVE: This randomized clinical trial aimed to compare the efficacy of an oral irrigator and an interdental brush in patients with peri-implant mucositis clinically and biochemically at different time points (at baseline and at the 2nd, 4th, and 12th weeks). MATERIALS AND METHODS: Forty-five patients with at least one implant with peri-implant mucositis were included in the present study (n = 45). The patients were divided into three groups: oral irrigator + toothbrush (OI group, n = 15), interdental brush + toothbrush (IB group, n = 15), and toothbrush only (control) (C group, n = 15). The modified plaque index (mPlI), modified sulcus bleeding index (mSBI), probing pocket depth (PPD), probing attachment level (PAL), and bleeding on probing (BOP) were recorded at baseline and at the 2nd, 4th, and 12th weeks. The levels of interleukin 1 beta (IL-1ß), transforming growth factor-beta (TGF-ß), tissue-type plasminogen activator (t-PA), and plasminogen activator inhibitor-1 (PAI-1) were also determined in the peri-implant crevicular fluid samples biochemically. RESULTS: The mSBI and t-PA at the 2nd week (p = 0.003; p = 0.003); the mPlI, mSBI, BOP, t-PA, and PAI-1 at the 4th week (p < 0.05; p < 0.001; p < 0.001; p = 0.015; p = 0.011); and the mPlI, mSBI, IL-1ß, t-PA, and PAI-1 at the 12th week (p < 0.05; p < 0.001; p = 0.013; p < 0.001; p = 0.002) were significantly lower in the OI group compared with those in the C group. Meanwhile, PAI-1 at the 2nd week, mSBI at the 4th week, and t-PA at the 12th week were significantly lower in the OI group compared with those in the IB group (p < 0.001; p = 0.011; p = 0.003). At the 2nd, 4th, and 12th weeks, all other parameters were not statistically different in the three groups. CONCLUSION: The clinical indexes (such as mSBI and BOP) that play an important role in the diagnosis of peri-implant mucositis showed the lowest means (although limited) in the OI group at all evaluation time points. Moreover, when the clinical and biochemistry results were interpreted altogether, it became apparent that the OI group exhibited similar or more effective results than the IB group in resolving peri-implant mucositis. In light of the foregoing, this study concluded that the use of an oral irrigator can be as effective as an interdental brush in interdental cleaning. CLINICAL RELEVANCE: In this study, it is suggested that the regular use of an oral irrigator along with a toothbrush could be an appropriate alternative to other oral hygiene products such as dental floss and interdental brush for the management of peri-implant mucositis by preventing the accumulation of dental plaque (NCT03844035).


Asunto(s)
Implantes Dentales , Mucositis , Periimplantitis , Índice de Placa Dental , Humanos , Cepillado Dental
3.
Oral Health Prev Dent ; 18(1): 815-822, 2020 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-32895666

RESUMEN

PURPOSE: A novel acute-phase protein, YKL-40, is known as an inflammation-associated glycoprotein. YKL-40 is shown to be linked to inflammation, endothelial dysfunction and tissue remodeling secreted by various cells and is also considered to be stimulated by cytokines such as interleukin-6 (IL-6). The present study aimed to investigate YKL-40 and IL-6 levels in saliva and gingival crevicular fluid (GCF) of patients with chronic periodontitis (CP) after non-surgical periodontal therapy for the first time. MATERIALS AND METHODS: Twenty-six CP patients and 26 periodontally healthy individuals were enrolled. Clinical measurements were recorded; saliva and GCF samples were obtained at baseline and 1 and 3 months after non-surgical periodontal therapy. Levels of YKL-40 and IL-6 in saliva and GCF were analysed by ELISA. RESULTS: Salivary and GCF YKL-40 and IL-6 levels were found to be statistically significantly higher in CP patients compared to healthy controls at baseline (p < 0.001). At 1 and 3 months after the completion of treatment, both YKL-40 and IL-6 levels in saliva and GCF had statistically significantly decreased compared with baseline values in CP patients (p < 0.001). On the other hand, no statistically significant difference was observed between 1 and 3 months in terms of salivary and GCF YKL-40 and IL-6 levels or any of the clinical findings (p > 0.016). CONCLUSION: Salivary and GCF YKL-40 levels may be useful to evaluate resolution of periodontal inflammation. Within the limits of this study, YKL-40 acute-phase protein might be a potential biomarker for detection of periodontitis and monitoring the response to periodontal therapy.


Asunto(s)
Periodontitis Crónica , Líquido del Surco Gingival/química , Proteína 1 Similar a Quitinasa-3 , Humanos , Interleucina-6/análisis , Saliva/química
4.
J Oral Sci ; 61(4): 554-557, 2019 Nov 27.
Artículo en Inglés | MEDLINE | ID: mdl-31588098

RESUMEN

The purpose of this study was to evaluate the effects of administered Paeoniflorin (Pae) on periodontal tissues within an experimental periodontitis model. Forty male Wistar rats were used in this study and experimental periodontitis was created in all rats except in the control group (n = 10, first group). In the periodontitis group, experimental periodontitis was created but no other application was performed (n = 10, second group). In the other groups created experimental periodontitis, systemic Pae (n = 10, third group) or saline (n = 10, fourth group) was applied. A biochemical analysis of the gingival vascular endothelial growth factor (VEGF) levels and a histomorphometric analysis (measurements of the area of alveolar bone, alveolar bone resorption, and attachment loss) were performed. In the Pae group, the area of the alveolar bone was increased, while alveolar bone resorption and attachment loss decreased. Gingival VEGF levels increased in all groups that created experimental periodontitis and the greatest increase seen in the Pae group. Histomorphometric and biochemical analyses in this study suggest that Pae has a curative effect on periodontal tissues. However, additional studies are needed to confirm these results.


Asunto(s)
Pérdida de Hueso Alveolar , Periodontitis , Animales , Glucósidos , Masculino , Monoterpenos , Ratas , Ratas Wistar , Factor A de Crecimiento Endotelial Vascular
5.
Arch Oral Biol ; 102: 135-140, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-31005686

RESUMEN

OBJECTIVES: Appraise the effect of systemic Paeoniflorin (Pae) application on the periodontium during and after induction of experimental periodontitis in the presence of ligature and after its removal. DESIGN: Seventy male Wistar rats were separated into seven equal groups. The first group was reserved as healthy control group (Group 1: no periodontitis, no medication) and experimental periodontitis was induced with ligature in the remaining rats. In experimental periodontitis groups, Pae or saline was administered systemically in three differet periods; during the experimental periodontitis induction (period 1), after experimental periodontitis induction which ligature removed (period 2) or ligature kept in position (period 3). Only, one of the groups acted as the control periodontitis group and received no treatment. Experimental periodontitis groups were as follows; Group 2: medication in period 1, Group 3: periodontitis and no medication, Group 4: medication in period 2, Group 5: saline application in period 2, Group 6: medication in period 3, Group 7: saline application in period 3. Matrix metalloproteinases-9 (MMP-9) levels and interleukin-10 (IL-10) levels were detected biochemically and histomorphometric analyses were performed. These analyses included measurements of the area of alveolar bone, the level of alveolar bone, and attachment loss. RESULTS: Area of alveolar bone and IL-10 levels were higher in the Pae-administered groups; level of alveolar bone, attachment loss, and MMP-9 levels were correspondingly lower (P < 0.05). The beneficial effects at histomorphometrical and biochemical levels of Pae were the strongest in the rats that were administered Pae after the removal of ligature. CONCLUSIONS: Systemically administered Pae had a positive effect on the healing of periodontal tissues. Pae can be used as a new therapeutic agent for periodontal diseases, but microbiology-based studies and more extensive biochemistry-based experimental and clinical studies are needed to address this possibility.


Asunto(s)
Periodontitis , Pérdida de Hueso Alveolar , Animales , Glucósidos , Masculino , Monoterpenos , Ratas , Ratas Wistar
6.
J Oral Sci ; 58(4): 491-499, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-28025432

RESUMEN

The purpose of this study was to evaluate visfatin levels at different stages of periodontal disease and in healthy tissues. In addition, the effect of non-surgical periodontal therapy on visfatin levels in gingival crevicular fluid and serum was investigated. Forty-five patients were divided into three groups based on clinical and radiographical findings. Group 1 comprised periodontally healthy individuals (n = 15); group 2 comprised patients with gingivitis (n = 15); and group 3 was composed of patients with generalized chronic periodontitis (n = 15). Gingival crevicular fluid and serum samples were collected before treatment and at 1, 3, and 6 months after treatment. Visfatin levels were measured by enzyme-linked immunosorbent assays. Gingival crevicular fluid and serum visfatin levels were higher in patients with chronic periodontitis than those with gingivitis or healthy controls (P < 0.016). In addition, visfatin levels were higher in the gingivitis group than in healthy controls (P < 0.016). Non-surgical periodontal treatment resulted in a significant reduction in gingival crevicular fluid and serum visfatin levels. Furthermore, visfatin levels increased with inflammation and decreased following periodontal treatment. Our findings suggest that visfatin is an inflammatory biomarker of periodontal disease.(J Oral Sci 58, 491-499, 2016).


Asunto(s)
Biomarcadores/metabolismo , Líquido del Surco Gingival/metabolismo , Nicotinamida Fosforribosiltransferasa/metabolismo , Enfermedades Periodontales/terapia , Adulto , Biomarcadores/sangre , Femenino , Humanos , Masculino , Persona de Mediana Edad , Nicotinamida Fosforribosiltransferasa/sangre
7.
J Periodontal Implant Sci ; 46(2): 84-95, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-27127689

RESUMEN

PURPOSE: The objective of this study was to investigate the effect of a dietary flavonoid, kaempferol, which has been shown to possess antiallergic, anti-inflammatory, anticarcinogenic, and antioxidant activities on the periodontium by histomorphometric analysis and on gingival tissue matrix metalloproteinase-1 (MMP-1), MMP-8, and tissue inhibitor of metalloproteinase-2 (TIMP-2) by biochemical analysis of rats after experimental periodontitis induction. METHODS: Sixty Wistar rats were randomly divided into six groups of ten rats each, and silk ligatures were placed around the cervical area of the mandibular first molars for 15 days, except in the healthy control rats. In the experimental periodontitis groups, systemic kaempferol (10 mg/kg/2d) and saline were administered by oral gavage at two different periods (with and without the presence of dental biofilm) to all rats except for the ten non-medicated rats. Alveolar bone area, alveolar bone level, and attachment level were determined by histomorphometric analysis, and gingival tissue levels of MMP-1, MMP-8, and TIMP-2 were detected by biochemical analysis. RESULTS: Significantly greater bone area and significantly less alveolar bone and attachment loss were observed in the kaempferol application groups compared to the control groups (P<0.05). In addition, gingival tissue MMP-1 and -8 levels were significantly lower in the kaempferol application groups compared to the control groups and the periodontitis group (P<0.001). There were no statistically significant differences in TIMP-2 levels between the kaempferol and saline application groups (P>0.05). CONCLUSIONS: Kaempferol application may be useful in decreasing alveolar bone resorption, attachment loss, and MMP-1 and -8 production in experimental periodontitis.

8.
J Periodontol ; 85(9): 1240-6, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24635545

RESUMEN

BACKGROUND: YKL-40, a new acute-phase protein, is shown to be elevated in inflammatory diseases, such as rheumatoid arthritis, type 2 diabetes mellitus, and coronary artery diseases. However, there is no data indicating a relationship between YKL-40 and periodontal disease. Interleukin-6 (IL-6) is the major regulator of acute-phase protein synthesis and one of the most studied inflammatory markers in periodontal disease. The purpose of the present study is to evaluate YKL-40 and IL-6 levels in gingival crevicular fluid (GCF) and serum of patients with periodontal disease and healthy individuals. METHODS: Periodontally healthy individuals (n = 15), patients with gingivitis (n = 15), and patients with severe chronic periodontitis (CP) (n = 15) without any systemic disease were included in the study. Clinical measurements were recorded; GCF and blood samples were obtained from each participant. GCF and serum YKL-40 and IL-6 levels were analyzed by enzyme-linked immunosorbent assay. Statistical analysis was performed by parametric and non-parametric tests. RESULTS: Total amounts of YKL-40 and IL-6 in GCF as well as serum YKL-40 and IL-6 levels were significantly higher in patients with gingivitis and CP compared with healthy controls (P <0.01). YKL-40 levels in GCF and serum as well as serum IL-6 levels were significantly higher in patients with CP compared with patients with gingivitis (P <0.01). CONCLUSIONS: YKL-40 levels in GCF as well as serum YKL-40 and IL-6 levels increased from gingivitis to periodontitis. Within the limits of the present study, the YKL-40 molecule might be a potential novel inflammatory marker of periodontal disease.


Asunto(s)
Proteínas de Fase Aguda/análisis , Adipoquinas/análisis , Autoantígenos/análisis , Periodontitis Crónica/inmunología , Gingivitis/inmunología , Interleucina-6/análisis , Lectinas/análisis , Adipoquinas/sangre , Adulto , Pérdida de Hueso Alveolar/inmunología , Autoantígenos/sangre , Biomarcadores/análisis , Biomarcadores/sangre , Proteína 1 Similar a Quitinasa-3 , Periodontitis Crónica/sangre , Índice de Placa Dental , Femenino , Líquido del Surco Gingival/inmunología , Gingivitis/sangre , Humanos , Mediadores de Inflamación/análisis , Interleucina-6/sangre , Lectinas/sangre , Masculino , Persona de Mediana Edad , Pérdida de la Inserción Periodontal/inmunología , Índice Periodontal , Bolsa Periodontal/inmunología
9.
J Periodontol ; 85(1): 178-87, 2014 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-23646851

RESUMEN

BACKGROUND: The aim of this study is to examine, for the first time, the role of systemic and local atorvastatin application on periodontium using histomorphometric and immunohistochemical analysis during and after experimental periodontitis induction with or without the presence of microbial dental biofilm. METHODS: One hundred ten male Wistar rats were used. Silk ligatures were placed around the cervical area of the mandibular first molars; rats in the healthy control group received no ligatures (n = 10). In experimental periodontitis groups (n = 90), systemic and local atorvastatin and saline were administered in three different periods; the control periodontitis group (n = 10) received no treatment. Histomorphometric analysis, which included alveolar bone area, alveolar bone level, and attachment loss, and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP)-9, were performed after the rats were sacrificed at the end of the experimental procedure. RESULTS: There was a greater increase in alveolar bone area and VEGF immunoreactivity, as well as a greater decrease in alveolar bone and attachment loss and MMP-9 immunoreactivity, with systemic and local atorvastatin application during and after induction of experimental periodontitis. Local atorvastatin application showed better results on periodontium with regard to alveolar bone findings. CONCLUSIONS: Systemic and local atorvastatin application showed beneficial effects on periodontium during and after induction of experimental periodontitis. Within the limits of this study, it can be concluded that atorvastatin, which is used for hypercholesterolemia treatment, can also be used as a protective and therapeutic agent for periodontal disease.


Asunto(s)
Ácidos Heptanoicos/uso terapéutico , Inhibidores de Hidroximetilglutaril-CoA Reductasas/uso terapéutico , Metaloproteinasa 9 de la Matriz/análisis , Periodontitis/tratamiento farmacológico , Pirroles/uso terapéutico , Factor A de Crecimiento Endotelial Vascular/análisis , Administración Oral , Administración Tópica , Pérdida de Hueso Alveolar/tratamiento farmacológico , Pérdida de Hueso Alveolar/prevención & control , Proceso Alveolar/efectos de los fármacos , Proceso Alveolar/metabolismo , Animales , Atorvastatina , Ácidos Heptanoicos/administración & dosificación , Inhibidores de Hidroximetilglutaril-CoA Reductasas/administración & dosificación , Procesamiento de Imagen Asistido por Computador/métodos , Masculino , Metaloproteinasa 9 de la Matriz/efectos de los fármacos , Pérdida de la Inserción Periodontal/tratamiento farmacológico , Pérdida de la Inserción Periodontal/prevención & control , Ligamento Periodontal/efectos de los fármacos , Ligamento Periodontal/metabolismo , Periodontitis/prevención & control , Sustancias Protectoras/administración & dosificación , Sustancias Protectoras/uso terapéutico , Pirroles/administración & dosificación , Ratas , Ratas Wistar , Factor A de Crecimiento Endotelial Vascular/efectos de los fármacos
10.
Acta Odontol Scand ; 72(2): 92-8, 2014 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-24279607

RESUMEN

OBJECTIVE: The purpose of this clinical investigation was to evaluate long-term results obtained with the combination of platelet pellet (PP) plus bioabsorbable barrier membrane (BM) and to compare this outcome with the results obtained using bioactive glass (BG) graft material with a BM. MATERIALS AND METHODS: Using a split mouth design, 11 chronic periodontitis patients (power ≥ at least 80%) were randomly assigned to treatment with a combination of PP/GTR or BG/GTR in contra-lateral dentition areas. Clinical attachment level (CAL) as the primary outcome variable, calculated as the sum of probing pocket depth (PPD) and gingival recession, and radiological alveolar bone level were recorded at baseline, 6 months and 5 years. RESULTS: There were no statistical differences between test and control defects at baseline. PPD reductions and CAL and radiological alveolar bone height gains were statistically significant between baseline and 6 months and between baseline and 5 years in both groups (p < 0.01). Six months results of frequency distribution showed that 82% of the defects attained ≥ 4 mm CAL gain in both groups, while 5 year results showed that 73% of the defects attained 2 mm ≤ CAL gain < 4 mm in the PP/BM group and 55% of the defects attained 2 mm ≤ CAL gain < 4 mm in the BG/BM group. All parameters evaluated showed no significant differences between 6 months and 5 years in both groups (p > 0.05). No statistically significant difference in any of the clinical parameters was observed at 6 months and 5 years between the groups (p > 0.05). CONCLUSIONS: The long-term efficacy of platelet concentrate combined with a barrier membrane is similar with the combination of bioactive glass graft material and barrier membrane, suggesting that results obtained with both treatment approaches can be maintained over a period of 5 years.


Asunto(s)
Plaquetas , Periodoncio/anomalías , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Periodoncio/diagnóstico por imagen , Radiografía
11.
Eur J Dent ; 7(1): 6-14, 2013 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-23408239

RESUMEN

OBJECTIVE: The aim of this 6-month prospective randomized clinical study was to compare the effectiveness of autogenous cortical bone (ACB) and bioactive glass (BG) grafting for the regenerative treatment of intraosseous periodontal defects. METHODS: Via a split-mouth design, 15 chronic periodontitis patients (7 men, 8 women; mean age, 43.47 ± 1.45 years) who had probing pocket depths (PPDs) of ⩾6 mm following initial periodontal therapy were randomly assigned to receive 2 treatments in contralateral areas of the dentition: ACB grafting and BG grafting. The parameters compared in the patients were preoperative and 6-month postoperative PPDs, clinical attachment levels (CALs), and radiographic alveolar bone heights. RESULTS: Both treatment modalities resulted in significant changes in postoperative measurements when compared to preoperative values (p < 0.01). PPDs were decreased, CALs were increased, and radiographic alveolar bone heights were increased by 5.00 ± 0.28, 4.60 ± 0.21, and 5.80 ± 0.43 mm in patients treated with ACB grafting and 5.13 ± 0.32, 4.67 ± 0.27, and 5.33 ± 0.36 mm in patients treated with BG grafting, respectively. Differences between the treatments were not statistically significant (P>.05). CONCLUSIONS: Within the limitations of this study, both ACB and BG grafting led to significant improvements in clinical and radiographic parameters 6 months postoperatively. These results suggest that either an ACB graft, which is completely safe with no associated concerns about disease transmission and immunogenic reactions, or a BG graft, which has an unlimited supply, can be selected for regenerative periodontal treatment.

12.
J Periodontol ; 84(11): 1673-82, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-23289868

RESUMEN

BACKGROUND: The aim of this study is to compare gingival changes induced by short- and long-term tacrolimus and nifedipine administration, alone or in combination, and evaluate the expression levels of tumor suppressor phosphatase and tensin homolog (PTEN) in drug-induced gingival overgrowth. METHODS: Eighty rats were equally divided into eight groups: 1) tacrolimus for 8 weeks; 2) nifedipine for 8 weeks; 3) tacrolimus and nifedipine for 8 weeks; 4) 8-week control; 5) tacrolimus for 24 weeks; 6) nifedipine for 24 weeks; 7) tacrolimus and nifedipine for 24 weeks; and 8) 24-week control. Histomorphometric analyses included measurements of epithelial thickness, connective tissue thickness, and height. Stereologic analyses included measurements of volumetric densities of fibroblasts (Vf), collagen fibers (Vcf), and blood vessels (Vbv). In addition, PTEN expression was analyzed using immunohistochemistry. RESULTS: Epithelial thickness and connective tissue thickness were significantly increased in groups 5, 6, and 7 compared to group 8 (P <0.05), whereas connective tissue height was significantly increased in groups 5 and 7 (P <0.001). Vf and Vcf were significantly increased in group 7 compared to group 8 (P <0.001). PTEN immunoreactivity was significantly decreased in all experimental groups compared to the control groups (P <0.05). CONCLUSIONS: Results suggest that duration of drug administration is a more important risk factor than drug combination. The results include a potentially new insight about PTEN's role in the etiology of drug-induced gingival overgrowth.


Asunto(s)
Bloqueadores de los Canales de Calcio/farmacología , Encía/efectos de los fármacos , Inmunosupresores/farmacología , Nifedipino/farmacología , Tacrolimus/farmacología , Animales , Vasos Sanguíneos/efectos de los fármacos , Bloqueadores de los Canales de Calcio/administración & dosificación , Bloqueadores de los Canales de Calcio/efectos adversos , Recuento de Células , Colágeno/efectos de los fármacos , Tejido Conectivo/efectos de los fármacos , Tejido Conectivo/patología , Combinación de Medicamentos , Epitelio/efectos de los fármacos , Epitelio/patología , Fibroblastos/efectos de los fármacos , Fibroblastos/patología , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Encía/patología , Sobrecrecimiento Gingival/inducido químicamente , Inmunosupresores/administración & dosificación , Inmunosupresores/efectos adversos , Masculino , Nifedipino/administración & dosificación , Nifedipino/efectos adversos , Fosfohidrolasa PTEN/efectos de los fármacos , Fosfohidrolasa PTEN/genética , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Tacrolimus/administración & dosificación , Tacrolimus/efectos adversos , Factores de Tiempo
13.
Mediators Inflamm ; 2012: 809801, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22966213

RESUMEN

OBJECTIVE: Pentraxin 3 (PTX3), newly discovered inflammation marker, is a member of acute-phase proteins. The hypothesis, synthesis of gingival tissue and serum PTX-3 increases in the experimental periodontitis model (with 10-day and 40-day periods), was tested by detecting gingival tissue and serum PTX-3 levels in rats with experimental periodontitis. METHODS: Thirty rats were randomly divided into three groups of ten animals each: ligature-induced experimental periodontitis groups (with 10-day (Group1) and 40-day periods (Group2)) and healthy group (Group3). At the end of experimental period, rats were sacrificed, and radiological and histomorphometric analyses were performed on the mandibles. PTX3 levels were measured in gingival tissue and serum samples using ELISA. Plasma fibrinogen levels were measured according to the nephelometric method. RESULTS: Significant alveolar bone resorption and periodontal inflammation were evident in periodontitis groups. Levels of PTX3 in gingival tissue were statistically higher in Group 1 than those in groups 2 and 3 (P < 0.01). No significant difference was found in serum PTX3 levels between experimental periodontitis and control groups (P > 0.05). Plasma fibrinogen levels were significantly increased in the experimental periodontitis groups (P < 0.001). CONCLUSION: PTX3 seems to be associated with tissue destruction in earlier periods of inflammatory periodontal disease, contrary to the fibrinogen findings.


Asunto(s)
Proteína C-Reactiva/metabolismo , Fibrinógeno/metabolismo , Periodontitis/metabolismo , Componente Amiloide P Sérico/metabolismo , Animales , Tejido Conectivo/metabolismo , Modelos Animales de Enfermedad , Masculino , Periodoncio/metabolismo , Periodoncio/patología , Distribución Aleatoria , Ratas , Ratas Wistar
14.
Clin Oral Investig ; 16(1): 251-8, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-21086003

RESUMEN

The purpose of this study was to compare the effectiveness of mesenchymal stem cells (MSCs) with platelet-rich plasma (PRP) as scaffold and autogenous cortical bone (ACB) graft with and without PRP in the regenerative treatment of class II furcation defects in dogs. The mandibular second, third, and fourth premolars (P2, P3, P4) and maxillary P3 and P4 of both sides in three dogs were selected for experimentation. Class II furcation defects (5 mm in height and 2 mm in depth) were surgically created. Five weeks after the first operation, scaling + root planning (group 1), PRP (group 2), ACB (group 3), combination of ACB/PRP (group 4), and combination of MSCs/PRP (group 5) treatments were performed during open flap debridement. The percentage of cementum and alveolar bone formation was evaluated by histomorphometric analysis after a healing period of 8 weeks. There was new cementum along with periodontal ligament and coronal growth of alveolar bone in all groups. Cementum formation was significantly higher in groups 3, 4, and 5 compared to the control group (P < 0.05) with no significant difference between groups 2, 3, 4, and 5. Alveolar bone formation was similar in all groups (P > 0.05). It can be concluded that periodontal regeneration with complete filling of class II furcation defects with cementum, alveolar bone, and periodontal ligament is obtained 8 weeks after ACB, ACB/PRP, and MSCs/PRP treatments; however, efficacy of none is higher than another.


Asunto(s)
Trasplante Óseo/métodos , Defectos de Furcación/cirugía , Regeneración Tisular Guiada Periodontal/métodos , Trasplante de Células Madre Mesenquimatosas/métodos , Proceso Alveolar/patología , Animales , Diente Premolar/patología , Diente Premolar/cirugía , Células de la Médula Ósea/patología , Cementogénesis/fisiología , Colágeno , Desbridamiento , Cemento Dental/patología , Raspado Dental , Perros , Defectos de Furcación/clasificación , Defectos de Furcación/patología , Mandíbula/patología , Maxilar/patología , Osteogénesis/fisiología , Ligamento Periodontal/patología , Plasma Rico en Plaquetas/fisiología , Aplanamiento de la Raíz , Colgajos Quirúrgicos , Andamios del Tejido , Trasplante Autólogo , Resultado del Tratamiento
15.
Eur J Dent ; 4(4): 403-11, 2010 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-20922160

RESUMEN

OBJECTIVES: The aim of this clinical trial was to evaluate the additional benefit of using guided tissue regeneration (GTR) with autogenous cortical bone (ACB) grafting versus ACB grafting alone for the regenerative treatment of intraosseous periodontal defects. METHODS: Via a split-mouth design, 12 patients with chronic periodontitis (five men, seven women; mean age, 45.3±4.6 years) who had probing pocket depths (PPDs) of ≥6 mm following initial periodontal therapy were randomly assigned to two treatments in contralateral areas of the dentition: a combination of ACB grafting and GTR (with a absorbable membrane of polylactic acid) or ACB grafting alone. The compared parameters were preoperative and 6-month postoperative PPDs, clinical attachment levels (CALs), and radiographic alveolar bone heights. RESULTS: Both treatment modalities resulted in significant changes in the postoperative measurements from the preoperative values (P<.01). The reduction in the PPDs, gain in the CALs, and gain in the radiographic alveolar bone heights were 4.58±1.08, 4.25±1.06, and 5.50±2.24 mm in the patients treated with ACB grafting and GTR and 4.92±1.00, 4.50±0.80, and 5.92±1.83 mm in those treated with ACB grafting alone, respectively. The differences between the treatments were not statistically significant (P>.05). CONCLUSIONS: Within the study limitations, both ACB grafting with GTR and ACB grafting alone lead to significant improvements in clinical and radiographic parameters at 6 months postoperatively. The combined approach does not provide any additional benefit for treating intraosseous periodontal defects.

16.
Inflamm Res ; 59(7): 543-9, 2010 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-20127137

RESUMEN

OBJECTIVE AND DESIGN: The aim of this study was to determine vascular endothelial growth factor (VEGF) mRNA expression levels in gingival tissues of gingivitis and periodontitis patients with diabetes mellitus and those without. The hypothesis tested is that expression of VEGF, considered the effective cytokine in the relationship between diabetes and periodontal disease, is differentially affected in gingivitis and periodontitis patients with or without diabetes mellitus compared to healthy controls. METHODS: Forty-five subjects were evaluated in five groups; individuals with gingivitis (group 1; n = 10), individuals with periodontitis (group 2; n = 10), individuals with gingivitis + type II diabetes (group 3; n = 10), individuals with periodontitis + type II diabetes (group 4; n = 10), and individuals without periodontal and systemic disease (group 5; n = 5). VEGF mRNA levels in gingival tissues were measured by quantitative real-time PCR using Lightcycler. RESULTS: Expression of VEGF mRNA was detected in all groups. There was no significant difference in expression levels of VEGF mRNA between groups (P > 0.05). CONCLUSIONS: VEGF expression is probably related to both maintenance of periodontal health and periodontal tissue destruction. It can be concluded that systemic condition in type II diabetes mellitus under good metabolic control does not seem to have additional effects on gingival tissue VEGF mRNA levels in gingivitis and periodontitis patients.


Asunto(s)
Diabetes Mellitus/metabolismo , Encía/metabolismo , Gingivitis/metabolismo , Enfermedades Periodontales/metabolismo , Periodontitis/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismo , Adulto , Anciano , Diabetes Mellitus/patología , Encía/patología , Humanos , Persona de Mediana Edad , Enfermedades Periodontales/patología , ARN Mensajero/metabolismo , Factor A de Crecimiento Endotelial Vascular/genética
17.
Artículo en Inglés | MEDLINE | ID: mdl-19615653

RESUMEN

OBJECTIVE: The objective of this randomized controlled trial was to compare the effects of different suturing techniques (simple loop suture vs. anchor suture) on the periodontal health of the adjacent second molars after impacted mandibular third molar extraction. STUDY DESIGN: Fifteen patients with bilaterally identical impacted mandibular third molars were used. Using split-mouth design, flaps were closed with either anchor suture technique or simple loop suture technique after the extraction of third molars. Postoperative examination included probing pocket depth (PPD) and clinical attachment level (CAL) measured at the distal surface of the second molars before surgery and 6 months after surgery. RESULTS: The PPD and CAL in the distal surfaces of the second molars were significantly higher after 6 months in the simple suture group (P = .001), whereas no differences were found in the anchor suture group (P > .05). Intergroup comparisons showed that the 6-month PPD and CAL values of the distal surfaces were significantly higher in the simple suture group compared with the anchor suture group (P = .015). CONCLUSION: The results suggest that anchor suture might be a better technique to use to maintain healthy periodontium and to prevent periodontal problems after the extraction of impacted third molars.


Asunto(s)
Tercer Molar/cirugía , Bolsa Periodontal/prevención & control , Periodoncio/lesiones , Complicaciones Posoperatorias/prevención & control , Técnicas de Sutura/efectos adversos , Diente Impactado/cirugía , Adolescente , Humanos , Diente Molar , Índice Periodontal , Bolsa Periodontal/etiología , Periodoncio/fisiología , Periodoncio/cirugía , Complicaciones Posoperatorias/etiología , Estadísticas no Paramétricas , Extracción Dental/métodos , Resultado del Tratamiento , Cicatrización de Heridas , Adulto Joven
18.
Clin Oral Investig ; 13(4): 393-400, 2009 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-19101738

RESUMEN

The purpose of this study was to compare histological effectiveness of platelet pellet (PP), which has higher platelet content than platelet-rich plasma, and the combination of PP/guided tissue regeneration (GTR) for class II furcation defects in dogs. The mandibular second, third, and fourth premolars of both sides in four dogs were used. Class II furcation defects (5 mm in height and 2 mm in depth) were surgically created. Five weeks after the first operation, second premolars were treated with scaling and root planing (group 1); right third and fourth premolars received PP (group 2), and left premolars received the combination of PP/GTR (group 3). Percentage of cementum and alveolar bone formation were evaluated by histometric analysis after a healing period of 12 weeks. There was new cementum along with periodontal ligament and coronal growth of alveolar bone in all groups. Cementum formation was significantly higher in groups 2 and 3 compared to the control group (P < 0.05) with no significant difference between groups 2 and 3. Limited alveolar bone formation was statistically similar in all groups (P > 0.05). It is concluded that both PP and the combination of PP/GTR are effective in the treatment of class II furcation defects in dogs. PP thus appears to be a suitable alternative material in the regenerative periodontal therapy.


Asunto(s)
Plaquetas/fisiología , Defectos de Furcación/cirugía , Regeneración Tisular Guiada Periodontal/métodos , Implantes Absorbibles , Proceso Alveolar/patología , Animales , Diente Premolar/cirugía , Cementogénesis/fisiología , Colágeno , Tejido Conectivo/patología , Cemento Dental/patología , Raspado Dental , Perros , Defectos de Furcación/clasificación , Defectos de Furcación/patología , Membranas Artificiales , Osteogénesis/fisiología , Ligamento Periodontal/patología , Recuento de Plaquetas , Aplanamiento de la Raíz , Cicatrización de Heridas/fisiología
19.
J Periodontol ; 79(10): 1950-61, 2008 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-18834251

RESUMEN

BACKGROUND: The present study was designed to evaluate the effects of risedronate, one of the most potent bisphosphonates, on alveolar bone resorption and angiogenesis in rats with experimental periodontitis to identify dose-response curves and treatment durations that can be therapeutic for periodontal therapy versus those associated with osteonecrosis of the jaws. METHODS: Thirty-five rats, 25 with experimental periodontitis (groups 1 through 5) and 10 with healthy periodontium (groups 6 and 7), were divided into seven equal groups: group 1 received no treatment; groups 2 and 3 received risedronate, 0.1 and 1 mg/kg, respectively, for 3 weeks; groups 4 and 5 received risedronate, 0.1 and 1 mg/kg, respectively, for 8 weeks; and groups 6 and 7 received 0.9% NaCl for 3 and 8 weeks, respectively. Animals in groups 2 through 7 were administered treatment 5 days per week. After histologic processing, histomorphometric and stereologic analyses were carried out to estimate the number of blood vessels (NBV) and the volumetric densities of bone (Vb), marrow (Vm), osteoblasts (Vob), and osteoclasts (Voc). RESULTS: A total of 0.1 and 1 mg/kg risedronate for 3 weeks (groups 2 and 3) significantly increased Vb and Vob and decreased Vm more prominently in group 2 (P <0.001), whereas 1 mg/kg risedronate for 8 weeks (group 5) induced no significant improvement in these parameters compared to group 1 (P >0.05). No significant decrease in Voc was found in drug-administered groups compared to group 1 (P >0.05). A significant decrease in NBV (P <0.01) and positive correlation between NBV and Vb (r(2) = 0.941; P = 0.006) were found only in group 5. CONCLUSION: A short duration of risedronate administration may be useful in inhibiting bone resorption in periodontitis, whereas excessive dosages of the drug administered in longer durations can lead to impairment of bone formation and angiogenesis.


Asunto(s)
Pérdida de Hueso Alveolar/tratamiento farmacológico , Conservadores de la Densidad Ósea/uso terapéutico , Ácido Etidrónico/análogos & derivados , Neovascularización Fisiológica/efectos de los fármacos , Proceso Alveolar/efectos de los fármacos , Proceso Alveolar/patología , Animales , Vasos Sanguíneos/efectos de los fármacos , Vasos Sanguíneos/patología , Densidad Ósea/efectos de los fármacos , Conservadores de la Densidad Ósea/administración & dosificación , Médula Ósea/efectos de los fármacos , Médula Ósea/patología , Recuento de Células , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Ácido Etidrónico/administración & dosificación , Ácido Etidrónico/uso terapéutico , Procesamiento de Imagen Asistido por Computador , Masculino , Osteoblastos/efectos de los fármacos , Osteoblastos/patología , Osteoclastos/efectos de los fármacos , Osteoclastos/patología , Osteogénesis/efectos de los fármacos , Osteonecrosis/inducido químicamente , Pérdida de la Inserción Periodontal/tratamiento farmacológico , Pérdida de la Inserción Periodontal/patología , Periodontitis/tratamiento farmacológico , Periodontitis/patología , Periodoncio/efectos de los fármacos , Placebos , Distribución Aleatoria , Ratas , Ratas Wistar , Ácido Risedrónico , Factores de Tiempo
20.
J Periodontol ; 78(6): 1129-35, 2007 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-17539728

RESUMEN

BACKGROUND: The major role of vascular endothelial growth factor (VEGF), an angiogenic mediator, in promoting the progression or the healing of periodontal disease is still unclear. The aim of this study was to evaluate the VEGF expression in the destruction and healing stages of periodontal disease and to investigate the association between VEGF expression and vascularization with regard to the number and diameters of blood vessels. METHODS: Thirty rats were distributed equally into two test groups and a control group. Experimental periodontal disease was induced in the test groups by silk ligatures, which were kept in position for 40 days. On the 40th day, ligatures were removed from the healing group, whereas ligatures were left in position in the destruction group. On the 60th day, rats were sacrificed; histomorphometric and biochemical analyses were carried out to determine the number and diameters of blood vessels and the assessment of VEGF concentration by enzyme-linked immunosorbent assay, respectively. RESULTS: There was a statistically significant increase in the number of blood vessels in the healing group and in the diameters of blood vessels in the destruction group compared to the control group (P <0.001). In vivo VEGF expressions were highest in the healing group (P <0.001) and correlated significantly with the number of blood vessels (r(2) = 0.814; P = 0.001). CONCLUSION: VEGF expression may be related more to the healing stage of periodontal disease than to the destruction stage of the lesion.


Asunto(s)
Pérdida de Hueso Alveolar/metabolismo , Encía/irrigación sanguínea , Enfermedades Periodontales/metabolismo , Factor A de Crecimiento Endotelial Vascular/análisis , Animales , Vasos Sanguíneos/anatomía & histología , Masculino , Modelos Animales , Ratas , Cicatrización de Heridas
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