Cuticular Hydrocarbon Profiles of Himalayan Bumble Bees (Hymenoptera: Bombus Latreille) are Species-Specific and Show Elevational Variation.

Bumble bees are important pollinators in natural environments and agricultural farmlands, and they are in particular adapted to harsh environments like high mountain habitats. In these environments, animals are exposed to low temperature and face the risk of desiccation. The Eastern Himalayas are one of the recognized biodiversity hotspots worldwide. The area covers subtropical rainforest with warm temperature and high precipitation as well as high mountain ranges with peaks reaching up to 7,000 m, shaping a diverse floral and faunal community at the different elevational zones. To identify possible adaptation strategies, we investigated the cuticular hydrocarbon profiles of four bumble bee species occurring at different elevational ranges in Arunachal Pradesh, the northeastern most state in India. At 17 locations along an elevational gradient, we collected workers of two species from lower elevations (B. albopleuralis and B. breviceps; ~ 100 m - 3,000 m asl) and two species from higher elevations (B. prshewalskyi and B. mirus; ~ 2,800 m - 4,500 m asl). The CHC profiles of all four species showed a significant degree of variation in the composition of hydrocarbons, indicating species specificity. We also found clear correlation with elevation. The weighted mean chain length of the hydrocarbons significantly differed between the low and high elevation species, and the proportion of saturated hydrocarbons in CHC profiles significantly increased with the elevational range of the bumble bee species. Our results indicate that bumble bees living at high elevations reduce the risk of water loss by adapting their CHC composition on their cuticle, a phenomenon that has also been found in other insects like ants and fruit flies.

Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.

Artemisia vulgaris is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of Artemisia vulgaris essential oil (AVEO) from the fresh leaves of A. vulgaris grown in Manipur. The AVEO isolated by hydro-distillation from A. vulgaris were analyzed by gas chromatography/mass spectrometry and solid-phase microextraction-GC/MS to describe their volatile chemical profile. There were 47 components identified in the AVEO by GC/MS, amounting to 97.66% of the total composition, while 97.35% were identified by SPME-GC/MS. The prominent compounds present in AVEO analyzed by direct injection and SPME methods are found to be eucalyptol (29.91% and 43.70%), sabinene (8.44% and 8.86%), endo-Borneol (8.24% and 4.76%), 2,7-Dimethyl-2,6-octadien-4-ol (6.76% and 4.24%), and 10-epi-γ-Eudesmol (6.50% and 3.09%). The consolidated component in the leaf volatiles comes to the terms of monoterpenes. The AVEO exhibits antimicrobial activities against fungal pathogens such as Sclerotium oryzae (ITCC 4107) and Fusarium oxysporum (MTCC 9913) and bacterial cultures such as Bacillus cereus (ATCC 13061) and Staphylococcus aureus (ATCC 25923). The percent inhibition of AVEO against the S. oryzae and F. oxysporum was found up to 50.3% and 33.13%, respectively. The MIC and MBC of the essential oil tested for B. cereus and S. aureus were found to be (0.3%, 0.63%) and (0.63%, 2.5%), respectively. Finally, the results revealed that the AVEO characterized by the hydro-distillation and SPME extraction yielded the same chemical profile and showed potent antimicrobial activities. Further research into A. vulgaris's antibacterial properties can be performed in order to use it as a source for natural antimicrobial medications.

Geographical distribution of the giant honey bee Apis laboriosa Smith, 1871 (Hymenoptera, Apidae).

Worldwide pollinator declines have dramatically increased our need to survey and monitor pollinator distributions and abundances. The giant honey bee, Apis laboriosa, is one of the important pollinators at higher altitudes of the Himalayas. This species has a restricted distribution along the Himalayas and neighbouring mountain ranges of Asia. Previous assessments of its distribution, published more than 20 years ago, were based on museum specimens. Since then, 244 additional localities have been revealed through field trips by the authors, publications, and websites. We present a revised distribution for A. laboriosa that better defines its range and extends it eastward to the mountains of northern Vietnam, southward along the Arakan Mountains to west-central Myanmar, into the Shillong Hills of Meghalaya, India, and northwestward in Uttarakhand, India. This species is generally found at elevations between 1000-3000 m a.s.l.. In northeastern India A. laboriosa colonies occur during summer at sites as low as 850 m a.s.l. and some lower elevation colonies maintain their nests throughout the winter. Finally, we report three regions in Arunachal Pradesh, India, and nine locations in northern Vietnam, where we observed workers of A. laboriosa and A. dorsata foraging sympatrically; their co-occurrence supports the species status of Apis laboriosa.

Tangsa and Wancho of North-East India Use Animals not only as Food and Medicine but also as Additional Cultural Attributes.

Cultural and ritual uses of animals beyond those for food and medicine should not be dismissed if we wish to understand the pressure that wildlife is under. We documented such uses for the Tangsa and Wancho tribals of Eastern Arunachal Pradesh (India). Group discussions with assembled members of 10 accessible villages in each of the tribal areas were carried out in 2015 and 2016. Vernacular names of culturally important species were noted and details of hunting practices were recorded. The different uses of animals and their parts during rituals and festivals and their significance in decorations and adornments, in supernatural beliefs and in connection with tribal folklore (stories) are documented. Folklore helps us understand why some species are hunted and consumed while others for no apparent reason are killed or simply ignored. Similarities as well as differences between the two tribes were recorded and possible reasons for the differences are given. The roles that the government as well as the tribal leaders play to halt or slow down the erosion and gradual disappearance of traditions that define the two cultures without losing already rare and endangered species are highlighted.

Species composition and elevational distribution of bumble bees (Hymenoptera, Apidae, Bombus Latreille) in the East Himalaya, Arunachal Pradesh, India.

The East Himalaya is one of the world's most biodiverse ecosystems. However, very little is known about the abundance and distribution of many plant and animal taxa in this region. Bumble bees are a group of cold-adapted and high elevation insects that fulfil an important ecological and economical function as pollinators of wild and agricultural flowering plants and crops. The Himalayan mountain range provides ample suitable habitats for bumble bees. Systematic study of Himalayan bumble bees began a few decades ago and the main focus has centred on the western region, while the eastern part of the mountain range has received little attention and only a few species have been verified. During a three-year survey, more than 700 bumble bee specimens of 21 species were collected in Arunachal Pradesh, the largest of the north-eastern states of India. The material included a range of species that were previously known from a limited number of collected specimens, which highlights the unique character of the East Himalayan ecosystem. Our results are an important first step towards a future assessment of species distribution, threat, and conservation. Clear elevation patterns of species diversity were observed, which raise important questions about the functional adaptations that allow bumble bees to thrive in this particularly moist region in the East Himalaya.

Rats: if you can't beat them eat them! (Tricks of the trade observed among the Adi and other North-East Indian tribals).

BACKGROUND: Since outside the tribal areas of North-East India it is not widely known, neither in the world nor in India itself, that rats are considered a delicious food item, this was one of several reasons why we decided to present this ethnographic account of rat procurement and preparation (together with some additional comments on the cultural role that rats have especially amongst members of the Adi tribe). Consumption of rats by humans as a biological control method far superior to the use of rodenticide poisoning and rat consumption as a way to reduce hunting pressure on rare wild animals were further considerations to publish this account. METHODS: Semi-structured interviews were conducted with male and female members of eight tribal communities in Arunachal Pradesh (North-East India) on the uses of rats as food and as cultural objects. The construction of rat traps as well as the preparation of rat dishes were observed and recorded photographically. RESULTS: Numerous species of small rodents, collectively called "rats" by the locals of North-East Indian tribes and comprising the species Rattus rattus Linnaeus, R. nitidus Hodgson, R. burrus Miller, R. tanezumi Temminck as well as Bandicota bengalensis Gray and Hardwicke, B. indica Bechstein, and Mus musculus Linnaeus, are regularly trapped and consumed in roasted, cooked or smoked form. In this well-illustrated report the kinds of devices used to catch these animals are described and information is provided on how to prepare rats for human consumption. The role that rats as food and gift-exchange items play in the context of local culture is explained and the locals' most highly appreciated meat dish, known as bule-bulak oying and consisting of boiled rat's tail, legs and inner organs, is introduced. CONCLUSION: Given the need to meet the world's future food demands and the environmental consequences of an expanding livestock production with regard to global warming, water availability, deforestation, soil erosion etc., rats as a food item, as our example shows, should not be overlooked. Using rats as food reduces hunting pressures on other wild and often already rare animals. It is a far superior method to control rat populations than poisoning the rodents and the artisanal construction of rat traps by local menfolk helps maintaining traditional skills and knowledge.

Comparative survey of entomophagy and entomotherapeutic practices in six tribes of eastern Arunachal Pradesh (India).

A consolidated list of edible insects used in the eastern part of Arunachal Pradesh (N.E. India) by Wangcho (Wancho) and Nocte tribes of the Tirap District and the Shingpo, Tangsa, Deori and Chakma of the Changlang District has been prepared. The list is based on thorough, semi-structured field-interviews with 20 informants of each tribal group. At least 51 insect species, belonging to 9 orders were considered edible. The largest number of the edible species belonged to the Coleoptera (14), followed by 10 each of the Orthoptera and Hymenoptera, 9 of the Hemiptera, 3 Lepidoptera, 2 Isoptera and one each of Ephemeroptera, Odonata and Mantodea. As far as therapeutic uses of insects are concerned, 4 species (Hemiptera) were mentioned by the Wangcho (Wancho). Food insects are chosen by members of the various tribes according to traditional beliefs, taste, regional and seasonal availability of the insects. Depending on the species, only certain, but sometimes all, developmental stages are consumed. Preparation of the food insects for consumption involves mainly roasting or boiling. With the degradation of natural resources, habitat loss, rapid population growth, and increasing 'westernization' , the traditional wisdom of North-East Indian tribals related to insect uses is at risk of being lost.

Chemical composition of Aspongopus nepalensis Westwood 1837 (Hemiptera; Pentatomidae), a common food insect of tribal people in Arunachal Pradesh (India).

We identified nutritionally relevant fatty acids and macro- and micronutrient content in Aspongopus nepalensis Westwood 1837 (Hemiptera), an insect used as food by the ethnic people of Arunachal Pradesh (North East India). Regarding macronutrients, crude lipids made up 38.35 %, fibre 33.47 %, protein 10.6 %, moisture 41.9 %, and ash 2.1 %. Lipid amounts were higher than those of most other insects. A. nepalensis was rich in Na: 1.02 %, K: 0.35 %, Ca: 0.12 %, Mg: 0.16 % and moreover, contained considerable amounts of Fe, Cu, Zn, and Mn. With regard to vitamins, A was most abundant (34.38 µg/100 g); C, D, E, and those of the B-complex were present, but less abundant, and monounsaturated fatty acid (MUFA) content was high (56.1 % of the total fat). Oleic and palmitic acids (46.41 % and 32.32 % of total fat) were the two major fatty acids in this insect's oil. The latter also contained moderate amounts of stearic, palmitoleic, linoleic, myristic, eicosenoic, and linolenic acids.

Vertebrates used for medicinal purposes by members of the Nyishi and Galo tribes in Arunachal Pradesh (North-East India).

Arunachal Pradesh, the easternmost part of India, is endowed with diverse natural resources and inhabited by a variety of ethnic groups that have developed skills to exploit the biotic resources of the region for food and medicines. Information on animals and animal parts as components of folk remedies used by local healers and village headmen of the Nyishi and Galo tribes in their respective West Siang and Subansiri districts were obtained through interviews and structured questionnaires. Of a total of 36 vertebrate species used in treatments of ailments and diseases, mammals comprised 50%; they were followed by birds (22%), fishes (17%), reptiles (8%) and amphibians (3%). Approximately 20 common complaints of humans as well as foot and mouth disease of cattle were targets of zootherapies. Most commonly treated were fevers, body aches and pains, tuberculosis, malaria, wounds and burns, typhoid, smallpox, dysentery and diarrhoea, jaundice, and early pregnancy pains. Very few domestic animal species (e.g., goat and cattle) were used zootherapeutically. More frequently it was wild animals, including endangered or protective species like hornbill, pangolin, clouded leopard, tiger, bear, and wolf, whose various parts were either used in folk remedies or as food. Some of the animal-based traditional medicines or animal parts were sold at local markets, where they had to compete with modern, western pharmaceuticals. To record, document, analyze and test the animal-derived local medicines before they become replaced by western products is one challenge; to protect the already dwindling populations of certain wild animal species used as a resource for the traditional animal-derived remedies, is another.

Practices of entomophagy and entomotherapy by members of the Nyishi and Galo tribes, two ethnic groups of the state of Arunachal Pradesh (North-East India).

We prepared a consolidated list of edible and therapeutic insects used in Arunachal Pradesh (N.E. India) by two tribal societies (i.e., the Nyishi of East Kameng and the Galo of West Siang). The list is based on thorough, semi-structured field-interviews with 20 informants of each tribal group. At least 81 species of local insects, belonging to 26 families and five orders of insects, namely Coleoptera (24 species), Orthoptera (17 species), Hemiptera (16 species), Hymenoptera (15 species) and Odonata (9 species), are being used as food among members of these two indigenous societies. However, Nyishi use overall more species of insects as food than Galo people do and consume mostly Coleoptera and Hemiptera; amongst the Galo, on the other hand, Odonata and Orthoptera dominate. The selection of the food insects amongst the Nyishi and Galo is dictated by traditional tribal beliefs as well as the taste and availability of the insects. Depending on the species, only particular or all developmental stages are consumed. Some food insects may be included in the local diet throughout the year, others only when seasonally available. Commonly specimens are being prepared for consumption by roasting, frying or boiling. Twelve species of insects are deemed therapeutically valuable by the locals and are being used by the tribes investigated to treat a variety of disorders in humans and domestic animals. Members of the Galo use a greater number of insect species for remedial purposes than the Nyishi. With the degradation of natural resources, rapid population growth, and increasing influence of 'westernization', the traditional wisdom of entomophagy and entomotherapy is at risk of being lost. There is thus an urgent need to record the role insects play as components of local diets and folk remedies and to assess insect biodiversity in the light of these uses.