EPS-corona formation on graphene family nanomaterials (GO, rGO and graphene) and its role in mitigating their toxic effects in the marine alga Chlorella sp.

GFNs have widespread applications but can harm marine systems due to excessive use and improper disposal. Algae-secreted EPS can mitigate nanomaterial harm, but their impact on GFN toxicity is understudied. Hence, in the present study, we investigated the toxicity of three GFNs, graphene oxide (GO), reduced graphene oxide (rGO), and graphene, in pristine and EPS-adsorbed forms in the marine alga Chlorella sp. At an environmentally relevant concentration of 1 mgL-1, all three GFNs induced considerable oxidative stress and impeded growth and photosynthetic activity of the algae. The order of the toxic potential followed GO > rGO > graphene. The various facets of adsorption of EPS (1:1 mixture of loosely bound, and tightly bound EPS) on GFNs were investigated through microscopy, surface chemical analyses, fluorescence quenching studies, and isotherm and kinetics studies. Amongst the pristine GFNs treated with algal cells, GO was found to exert the maximum negative effects on algal growth. Upon adsorption of EPS over the GFNs, a significant decline in growth inhibition was observed compared to the respective pristine forms which strongly correlated with reduced oxidative stress and enhanced photosynthetic parameters in the cells. The formation of a layer of eco-corona after interaction of GFNs with EPS possibly caused a barrier effect which in turn diminished their toxic potential. The findings from the present investigation offer valuable insights into the environmental toxicity of GFNs and show that the eco-corona formation may lessen the risk posed by these materials in the marine environment.

Comparative toxicity assessment of individual, binary and ternary mixtures of SiO2, Fe3O4, and ZnO nanoparticles in freshwater microalgae, Scenedesmus obliquus: Exploring the role of dissolved ions.

Metal oxide nanoparticles (NPs) are considered among the most prevalent engineered nanomaterials. To have a deeper understanding of the mode of action of multiple metal oxide nanoparticles in mixtures, we have used a unicellular freshwater microalga Scenedesmus obliquus as a model organism. The toxicity of silicon dioxide (SiO2), iron oxide (Fe3O4), and zinc oxide (ZnO) NPs was studied individually as well as in their binary (SiO2 + Fe3O4, Fe3O4 + ZnO, and ZnO + SiO2) and ternary (SiO2 + Fe3O4 + ZnO) combinations. The effects of metal ions from ZnO and Fe3O4 were investigated as well. The results observed from the study, showed that a significant amount of toxicity was contributed by the dissolved ions in the mixtures of the nanoparticles. Decreases in the cell viability, ROS generation, lipid peroxidation, antioxidant enzyme activity, and photosynthetic efficiency were analyzed. Among all the individual particles, ZnO NPs showed the maximum effects and increased the toxicities of the binary mixtures. The binary and ternary mixtures of the NPs clearly showed increased toxic effects in comparison with the individual entities. However, the ternary combination had lesser toxic effects than the binary combination of Fe3O4 + ZnO. The decline in cell viability and photosynthetic efficiency were strongly correlated with various oxidative stress biomarkers emphasizing the crucial role of reactive oxygen species in inducing the toxic effects. The findings from this study highlight the importance of evaluating the combinatorial effects of various metal oxide NPs as part of a comprehensive ecotoxicity assessment in freshwater microalgae.

Unmasking effects of masks: Microplastics released from disposable surgical face masks induce toxic effects in microalgae Scenedesmus obliquus and Chlorella sp.

During the COVID-19 pandemic billions of face masks were used since they became a necessity in everyone's lives. But these were not disposed properly and serve as one of the most significant sources of micro and nano plastics in the environment. The effects of mask leached plastics in aquatic biota remains largely unexplored. In this work, we quantified and characterized the released microplastics from the three layers of the mask. The outer layer of the face mask released more microplastics i.e., polypropylene than middle and inner layers. We investigated and compared the acute toxic effects of the released microplastics between Scenedesmus obliquus and Chlorella sp. The results showed a decrease in cell viability, photosynthetic yield, and electron transport rate in both the algal species. This was accompanied by an increase in oxidative stress markers such reactive oxygen species (ROS) and malondialdehyde (MDA) content. There was also a significant rise of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) in both the algal cells. Furthermore, morphological changes like cell aggregation and surface chemical changes in the algae were ascertained by optical microscopy and FTIR spectroscopy techniques, respectively. The tests confirmed that Scenedesmus obliquus was more sensitive than Chlorella sp. to the mask leachates. Our study clearly revealed serious environmental risk posed by the released microplastics from surgical face masks. Further work with other freshwater species is required to assess the environmental impacts of the mask leachates.

Nano-SiO2 transport and retention in saturated porous medium: Influence of pH, ionic strength, and natural organics.

Nano silica (nSiO2), induces potential harmful effects on the living environment and human health. It is well established that SiO2 facilitates the co-transport of a variety of other contaminants, including heavy metals and pesticides. The current study focused on the systematic evaluation of the effects of multiple physicochemical parameters such as pH (5, 7, and 9), ionic strength (10, 50, and 100 mM), and humic acid (0.1, 1, and 10 mg/L) on the transport and retention of nSiO2 in saturated porous medium. Additionally, the influent concentration of nSiO2 (10, 50, and 100 mg/L) was also varied. Our experimental findings indicate that the size of nSiO2 aggregates was directly related to the pH, ionic strength, HA, and particle concentration had a significant impact on the breakthrough curves (BTCs). The stability provided by the varying concentrations of pH and humic acid had a significant effect on the size of nSiO2 aggregates and transport (C/C0 > 0.7). The presence of a greater magnitude of negative charge on the surface of both nSiO2 and quartz sand resulted in less aggregation and enhanced flow of nSiO2 through the sand column. The Electrostatic and steric repulsion forces were the primary governing mechanisms in relation to the size of nSiO2 aggregates, affecting the single-collector efficiency and attachment efficiency, which determined the maximal transport of nSiO2. Conversely, a probable increase in Van der Waals force of attraction exacerbated the particle deposition and reduced their mobility for high ionic strength, and particle concentrations (C/C0 < 0.1). The formation of large nSiO2 aggregates, in particular, was principally responsible for the enhancement of nSiO2 retention in sand columns over a broad range of IS and particle concentration. The interaction energy profiles based on the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory were determined to understand the mechanism of nSiO2 deposition. Aditionally, all the experimental BTCs were mathematically simulated and justified by the colloidal filtration theory (CFT). Considering the environmental ramifications, the transport behavior of nSiO2 was further evaluated in various natural matrices such as river, lake, ground, and tap water. The nSiO2 suspended in the river, lake, and tap water had significantly higher mobility (C/C0 > 0.7), whereas groundwater indicated higher retention (C/C0 < 0.3). The study advances our collective knowledge of physicochemical and environmental parameters that can affect particle mobility.

A comprehensive estimate of the aggregation and transport of nSiO2 in static and dynamic aqueous systems.

Silicon dioxide nanoparticles (nSiO2) are extensively used in diverse fields and are inevitably released into the natural environment. Their overall aggregation behaviour in the environmental matrix can determine their fate and ecotoxicological effect on terrestrial and aquatic life. The current study systematically evaluates multiple parameters that can influence the stability of colloidal nSiO2 (47 nm) in the natural aquatic environment. At first, the influence of several hydrochemical parameters such as pH (5, 7, and 9), ionic strength (IS) (10, 50, and 100 mM), and humic acid (HA) (0.1, 1, and 10 mg L-1) was examined to understand the overall aggregation process of nSiO2. Furthermore, the synergistic and antagonistic effects of ionic strength and humic acid on the transport of nSiO2 in the aqueous environment were examined. Our experimental findings indicate that pH, ionic strength, and humic acid all had a profound influence on the sedimentation process of nSiO2. The experimental observations were corroborated by calculating the DLVO interaction energy profile, which was shown to be congruent with the transport patterns. The present study also highlights the influence of high and low shear forces on the sedimentation process of nSiO2 in the aqueous medium. The presence of shear force altered the collision efficiency and other interactive forces between the nanoparticles in the colloidal suspension. Under the experimental stirring conditions, a higher abundance of dispersed nSiO2 in the upper layer of the aqueous medium was noted. Additionally, the transport behaviour of nSiO2 was studied in a variety of natural water systems, including rivers, lakes, ground, and tap water. The study significantly contributes to our understanding of the different physical, chemical, and environmental aspects that can critically impact the sedimentation and spatial distribution of nSiO2 in static and dynamic aquatic ecosystems.

Nanoplastics enhance the toxic effects of titanium dioxide nanoparticle in freshwater algae Scenedesmus obliquus.

The increased usage of titanium dioxide nanoparticles (nTiO2) in consumer products has led to their prevalence in freshwater systems. Nanoplastics, a secondary pollutant, can significantly influence the toxic effects of nTiO2 in freshwater organisms. The present study investigates the role of fluorescent nanoplastics (FNPs) in modifying the harmful effects of P25 nTiO2 in freshwater algae Scenedesmus obliquus. Three different concentrations of nTiO2, 0.025, 0.25, and 2.5 mg/L, were mixed with 1 mg/L of the FNPs to perform the mixture toxicity experiments. The presence of the FNPs in the mixture increased the toxicity of nTiO2 significantly. A significant increment in the oxidative stress parameters like total ROS, superoxide (O2∎-), and hydroxyl radical generation was observed for the mixture of nTiO2 with the FNPs in comparison with their individual counterparts. The lipid peroxidation, and the antioxidant enzyme activities in the algal cells correlated well with the reactive species generation results. The treatments with the binary mixture resulted in notable decrease in the esterase activity in the algal cells. The mixture toxicity results were further validated with Abbott's independent action model. Additionally, optical microscopic analysis and FTIR analysis were performed to study the morphological and surface chemical changes in the algae. This study demonstrated that the FNPs played a key role in enhancing the toxicity of nTiO2 in freshwater algae.

Mixture toxicity of TiO2 NPs and tetracycline at two trophic levels in the marine ecosystem: Chlorella sp. and Artemia salina.

Increasing usage of both nanomaterials and pharmaceuticals and their unabated release to the marine ecosystem pose a serious concern nowadays. The toxicity of the mixture of TiO2 NPs and tetracycline (TC) in the marine species are not very well covered in prior literature. The current study explores the joint toxic effects of TiO2 NPs and TC in a simulated marine food chain: Chlorella sp. and Artemia salina. Chlorella sp. was interacted with pristine TiO2 NPs (0.05, 05, and 5 mg/L), TC (0.5 mg/L), and their combinations for 48 h. The toxicity induced in Chlorella sp. by pristine TiO2 NPs through oxidative stress and chloroplast damage was not significantly changed in the presence of TC. Principal component analysis for the toxicity parameters revealed a strong association between growth inhibition and adsorption/internalization. In the second trophic level (A. salina), the waterborne exposure of TC additively increased the toxicity of TiO2 NPs. Both adsorption and degradation played a major role in the removal of TC from the suspension, resulting in additive toxic effects in both Chlorella sp. and A. salina. Compared to the waterborne exposure, the foodborne exposure of TiO2 NPs and TC induced lesser toxic effects owing to reduced uptake and accumulation in A. salina. Biomagnification results indicate that the dietary transfer of TiO2 NPs and TC does not pose a serious environmental threat in this two-level marine food chain.

Studies on the removal of acid violet 7 dye from aqueous solutions by green ZnO@Fe3O4 chitosan-alginate nanocomposite synthesized using Camellia sinensis extract.

In the present study, ZnO-Fe3O4 nanoparticles were synthesized using the leaves of Camellia sinensis and immobilized in crosslinked alginate-chitosan polymer beads and tested for their photocatalytic applications. The prepared nanocomposite was used for the simultaneous adsorption and photocatalytic degradation of acid violet 7 (AV7) dye. The optimization of reaction conditions ensured higher dye removal efficacy up to 94.21 ± 1.02% using the nanocomposite under UV-C irradiation of 365 nm. The kinetics of the adsorption study fitted well with the pseudo-first-order reaction. The Langmuir model fitted better to the adsorption isotherms compared to the Freundlich and Temkin models. The mechanism of degradation was studied by analyzing the treated AV7 solution. The removal efficiency in tap water, groundwater, and lake water was 83.23 ± 0.4%, 69.13 ± 1.6%, and 67.89 ± 0.3%, respectively. The residual toxicity of the degraded AV7 solution was tested on model organisms like freshwater algae, Scenedesmus sp., and plant model, Allium cepa, demonstrating the lower toxicity of the degraded AV7 product. Finally, a cost-benefit analysis of the experiments was also carried out.

Polystyrene nanoplastics diminish the toxic effects of Nano-TiO2 in marine algae Chlorella sp.

Widespread usage of nano-TiO2 in various commercial products and their consequent release into the seawater pose a severe threat to marine biota. Nanoplastics, a secondary pollutant in the marine environment, could influence adverse effects of nano-TiO2. The main goal of the present study was to investigate the influence of the differently functionalized polystyrene nanoplastics (COOH-PSNPs, NH2-PSNPs, and Plain-PSNPs) on the acute toxic effects of P25 nano-TiO2 in marine algae Chlorella sp. Three different concentrations of nano-TiO2, 0.25, 0.5, and 1 mg/L, mixed with 5 mg/L of the PSNPs were employed in this study. A substantial increase was noted in mean hydrodynamic sizes of nano-TiO2 when they were mixed with the PSNPs. This hetero-aggregation would reduce the bioavailability of the particles to the algae. The presence of the PSNPs in the mixture reduced the toxicity of nano-TiO2 significantly. A signficant decline in the oxidative stress parameters like total ROS, superoxide (), and hydroxyl radical generation was noted for the mixture of nano-TiO2 with the PSNPs in comparison with the pristine counterparts. The lipid peroxidation, and the antioxidant enzyme activities in the cells correlated well with the reactive species generation results. The treatments with the mixture resulted in notable enhancement in the esterase activity in the cells. The Independent Action model suggested antagonistic interactions between PSNPs and nano-TiO2. The results from this study clearly demonstrate that nano-TiO2 in presence of the PSNPs exerted significantly reduced cytotoxic effects in Chlorella sp, in comparison with the pristine particles.

Toxicity evaluation of nano-TiO2 in the presence of functionalized microplastics at two trophic levels: Algae and crustaceans.

The rising use of contaminants such as nanoparticles and microplastics has taken a heavy toll on the marine environment. However, their combined toxic effects on the species across various trophic levels remain quite unexplored. The aim of this study was to explore the effects of three surface-functionalized (carboxylated, plain, and aminated) polystyrene microplastics on nano-TiO2 toxicity across two trophic levels containing Chlorella sp. as the prey and Artemia salina as the predator. The experiments carried out on Chlorella sp. include the toxicity assessment, oxidative stress determination, and uptake of nano-TiO2 (both in the presence and absence of microplastics). Results revealed that the aminated and plain polystyrene microplastics enhanced nano-TiO2 toxicity, while carboxylated microplastics decreased the toxic effects in Chlorella sp. On the other hand, toxicity assessment in Artemia salina was carried out using two different modes of exposure: aqueous and dietary routes. The aqueous route involving the direct exposure of nano-TiO2 and microplastics indicated greater toxicity, uptake, and accumulation in Artemia salina than the dietary route of exposure. Since dietary exposure decreased the toxicity, uptake, and accumulation of nano-TiO2, no change (p > 0.05) in the biomagnification factors of nano-TiO2 was noted for all the test concentrations of nano-TiO2 combined with and without microplastics. The computed values were less than 1, indicating negligible transfer of nano-TiO2 from Chlorella sp. to Artemia salina. Overall, the study highlights the two-level trophic toxicity and the transfer potential of nano-TiO2 under the influence of different microplastics.

Development of biogenic bimetallic Pd/Fe nanoparticle-impregnated aerobic microbial granules with potential for dye removal.

The objective of this study was to develop bimetallic core-shell Pd/Fe nanoparticles on the surface of aerobic microbial granules (Bio-Pd/Fe) and to evaluate their dye removal potential using a representative dye, methyl orange (MO). The aerobic microbial granules (1.5 ± 0.32 mm) were grown for 70 days in a 3-L glass sequencing batch reactor (SBR) with a 12-h cycle time. The Bio-Pd/Fe formation was catalyzed by the Bio-H2 gas produced by the granules. The developed Bio-Pd/Fe was further used for MO removal from aqueous solutions, and the reaction parameters were optimized by response surface methodology (RSM). The XRD, SEM, EDAX, elemental mapping, and XPS studies confirmed the formation of Bio-Pd/Fe. Under the optimized removal conditions, 99.33% MO could be removed by Bio-Pd/Fe, whereas removal by Bio-Pd, Bio-Fe, aerobic microbial granules, and heat-killed granules were found to be quite low (68.91 ± 0.2%, 76.8 ± 0.3%, 19.8 ± 0.6%, and 6.59 ± 0.2%, respectively). The mechanism of removal was investigated by UV-visible spectroscopy, redox potential analysis, HR-LCMS analyses of the solution phase, and XRD and XPS analyses of the solid sorbent. The degradation products of MO exhibited m/z values corresponding to 292, 212, and 160 m/z. The remnant toxicity of the intermediate degradation products was analysed using freshwater algae, Scenedesmus sp. And Allium cepa, as indicator organisms. These assays suggested that after the treatment with Bio-Pd/Fe, MO was transformed to a lesser toxic form.

Interactive effects of micro/nanoplastics and nanomaterials/pharmaceuticals: Their ecotoxicological consequences in the aquatic systems.

Micro/nanoplastics are ubiquitous in the environment and cause pollution of the aquatic ecosystem, in particular, which is a serious concern worldwide. Micro/nanoplastics can act as a vector for multiple co-contaminants that co-exist in the aquatic environment. Apart from micro/nanoplastics, nanomaterials and pharmaceuticals are other emerging contaminants that can also raise severe problems. Thus, in this review, the physicochemical interactions occurring between micro/nanoplastics and nanomaterials and pharmaceuticals and the factors (chemical and environmental) affecting the sorption efficiency of nanomaterials and pharmaceuticals have been addressed. Furthermore, the influence of micro/nanoplastics on the bioavailability and toxic effects of nanomaterials and pharmaceuticals on both freshwater and marine species has been highlighted. Additional focus has also been given to study the mechanism of toxicity of the micro/nanoplastics-nanomaterials and pharmaceuticals complex on the different species of different trophic levels. Finally, this review addresses the knowledge gaps and provides insights into the future research strategies to better understand the interactive mechanisms between the binary contaminants and also the toxicity mechanisms of micro/nanoplastics and nanomaterials and pharmaceuticals.

Mitigating the toxic effects of CdSe quantum dots towards freshwater alga Scenedesmus obliquus: Role of eco-corona.

The extensive use of semiconducting nanoparticles such as quantum dots in biomedical and industrial products can lead to their inadvertent release into the freshwater system. Natural exudates in the aquatic system comprising extracellular polymeric substance (EPS) and protein-rich metabolites can eventually adsorb onto the quantum dots (QDs) surface and form an eco-corona. The alterations in the physio-chemical and toxicological behavior of CdSe/ZnS QDs under the influence of eco-corona in the freshwater system have not been explored yet. In the present study, lake water medium conditioned with exudate secreted by Scenedesmus obliquus was utilized as an eco-corona forming matrix. The time-based evolution of the eco-corona on the differently charged CdSe/ZnS QDs was analyzed using transmission electron microscopy and dynamic light scattering. Aging of amine-QDs in algal exudate for 72 h showed enhanced aggregation (Mean Hydrodynamic Diameter- 1969 nm) as compared to carboxyl-QDs (1543 nm). Further, eco-coronation tends to impart an overall negative charge to the QDs. The fluorescence intensity of amine-QDs was quenched by 84% due to the accumulation of higher eco-corona. An integrative effect of surface charge and accumulated eco-corona layer influenced the Cd2+ ion leaching from the QDs. An enhancement in the algal cell viability treated with carboxyl - CdSe/ZnS (90%) and amine- CdSe/ZnS QDs (94%) aged for 72 h suggested that eco-corona can effectively mitigate the inherent toxicity of the QDs. The oxidative stress markers in the algal cells (LPO, SOD, and CAT) were in correlation with the cytotoxicity results. The algal photosynthetic efficiency depended on the deposition of eco-coronated QDs on the cell surface. Cellular uptake results indicated low Cd2+ concentration of nearly 13.9 and 11.5% for carboxyl- and amine- CdSe/ZnS QDs respectively. This suggests that eco-coronation directly influences the bioavailability of engineered nanoparticles.

Eco-corona formation lessens the toxic effects of polystyrene nanoplastics towards marine microalgae Chlorella sp.

Unabated use of nanoplastics (<1 µm) in the consumer products and their consequent release to the marine environment poses a substantial threat to the marine ecosystem. The toxic impact of the nanoplastics on marine microalgae is yet to be explored in detail, and the role of reactive oxygen species generation remains largely unclear. The algal exudates constitute a significant part of the natural organics present in the marine system that may readily adsorb over the nanoplastics to form eco-corona. In the current work a marine alga, Chlorella sp., was considered a bioindicator organism and the effects of eco-corona formation in lessening the toxic impact of the nanoplastics was analyzed. Three differently functionalized polystyrene nanoplastics (PS NPs): Aminated (NH2-PS NPs), Carboxylated (COOH-PS NPs) and Plain nanoplastics were aged (12, 24, and 48 h) in the EPS containing medium to facilitate eco-corona formation. Decline in cell viability, membrane integrity, and photosynthetic yield were considered to be principle toxicity indicators. The role of oxidative stress as key mode of action (MOA) was studied considering generation of overall reactive oxygen species, and specific radicals (hydroxyl and superoxide) as relevant markers. The changes in antioxidant enzyme activities (superoxide dismutase, and catalase) were also measured. The results clearly indicate a significant decline in the oxidative stress and corresponding lessening of the toxic effects due to eco-corona formation on the PS NPs. The response varied with surface charge on the NPs and ageing duration. Considering the increasing importance of the nanoplastics as one of the major emerging pollutants in marine ecosystem, this study strongly suggests that the EPS mediated eco-corona formation may substantially lessen their toxic burden.

Combined effects of nano-TiO2 and hexavalent chromium towards marine crustacean Artemia salina.

There has been a significant increased concern of the impact of the toxicity of multiple contaminants in the marine environment. Thus, this study was aimed at determining whether the interaction between nano-TiO2 and Cr(VI) would modulate their toxic effects with the marine crustacean, Artemia salina. Nano-TiO2 agglomerated in artificial sea water (ASW) and readily formed micron-sized particles that settled down in the medium. The addition of Cr(VI) to nano-TiO2 aggravated their agglomeration through sorption of Cr(VI) onto nano-TiO2. This was reflected by a decrease in the residual concentration of Cr in the suspension. Acute toxicity tests performed using pristine nano-TiO2 (0.25, 0.5, 1, 2, and 4 mg/L) and Cr(VI) (0.125, 0.25, 0.5, and 1 mg/L) displayed a concentration dependent rise in the mortality of Artemia salina. To examine the effects of mixtures of nano-TiO2 and Cr(VI) on Artemia salina, two groups of experiments were designed. The former group studied the toxic effect of nano-TiO2 (0.5, 1, 2, and 4 mg/L) with a fixed concentration (0.125 mg/L) of Cr(VI). While the latter group studied the toxicity of Cr(VI) (0.25, 0.5, and 1 mg/L) with a fixed concentration (0.25 mg/L) of nano-TiO2. The toxic effects of nano-TiO2 was not significantly reduced at a fixed concentration of Cr(VI) but in contrast, a significant reduction in the Cr(VI) toxicity by fixed concentration of nano-TiO2 was observed. Toxicity data was well supported by an independent action model that proved the mode of action between nano-TiO2 and Cr(VI) to be antagonistic. Furthermore, ROS generation and measurement of antioxidant enzyme activities were also in line with toxicity results. From this study, the modification of Cr(VI) toxicity at fixed concentration of nano-TiO2 could have a huge impact on the reduction in Cr(VI) toxicity across trophic levels.

Drug loaded essential oil microemulsions enhance photostability and evaluation of in vitro efficacy.

Loss of stability of pharmaceutical APIs (Active Pharmaceutical Ingredient) due to photolytic activity has been a major concern in the pharmaceutical industry as it leads to loss of activity of API and excipients, the formation of toxic by-products, change in color and flavor. Itraconazole (ITZ) bulk drug was exposed to UVC (254 nm) irradiation in an environmental chamber (37 °C, 75 %RH) and its photoprotection by cinnamon, clove, eugenol, and oregano based microemulsion was analyzed. No significant change in the spectra was observed at various time points, confirming the photo-protective activity of microemulsions, unlike the bulk drug. FTIR spectra illustrate the fundamental peaks of the functional groups of ITZ and ITZ loaded MEs. The overlaid spectra showed that there was a minor change in peaks of UV exposed ITZ bulk drug but the ITZ loaded microemulsions were able to protect all the major functional groups. The in vitro anti-microbial assay against C. albicans demonstrated no significant change in the activity of ITZ loaded microemulsion between untreated, 7th day and 15th day while the activity of bulk drug was reduced drastically in the UVC exposed sample. It was concluded that microemulsions can be used as an effective photo-protective drug delivery vehicle for light-sensitive compounds.

A review on tetracycline removal from aqueous systems by advanced treatment techniques.

Tetracycline (TC), a frequently used drug for human and veterinary therapeutics, is among the most common antibiotic residues found in nature. Lack of advanced treatment techniques in the wastewater treatment plants (WWTPs) to remove residual TC from domestic and hospital wastewater poses a serious environmental risk. It is important to have an insight into the different advanced treatment techniques for efficient removal of TC from the surface water and in the WWTPs. The aim of this review is to discuss the nature and occurrence of TC in surface water and to present an overview of the various advanced treatment techniques for TC removal. The advanced treatment techniques include advanced oxidation processes (photolysis, ozonation, and catalytic/UV light-based degradation), membrane filtration, reverse osmosis, and adsorption techniques. Adsorption and integrated oxidation treatment techniques are the most widely studied methods, and they are widely accepted because of less cost, reusability, and toxic-free nature. Further, the uses of various types of catalysts for photodegradation and various sorbents for adsorption of TC are also presented. Finally, the importance of green nanocomposite for environmental sustainability in TC removal is emphasized.

Tetracycline affects the toxicity of P25 n-TiO2 towards marine microalgae Chlorella sp.

Pollutants such as n-TiO2 and tetracycline enter the marine environment through various sources starting from their production until disposal. Hence, it is vital to determine the interactive effect of one pollutant with the other when they coexist in the environment. In the present study, the effect of antibiotic - tetracycline (TC) on the toxicity of P25 n-TiO2 was studied with marine microalgae, Chlorella sp. The impact of TC (1 mg L-1) on five different concentrations of n-TiO2 (0.25, 0.5, 1, 2 and 4 mg L-1) under both visible and UV-A illuminations was evaluated. Effective diameter of n-TiO2 in ASW at 0th h increased from 690.69 ±â€¯19.55 nm (0.25 mg L-1) to 1183.04 ±â€¯37.10 nm (0.25 mg L-1 + 1 mg L-1) and 971.51 ±â€¯14.61 nm (4 mg L-1) to 1324.12 ±â€¯11.59 nm (4 mg L-1 + 1 mg L-1) in presence of TC. A significant increase in the toxicity of 4 mg L-1 n-TiO2 upon the addition of TC (68.16 ±â€¯0.37% under visible and 80.21 ±â€¯0.3% under UV-A condition) was observed. No significant difference in toxicity was observed between visible and UV-A illuminations. Further the toxicity data was corroborated through the measurement of oxidative stress and antioxidant enzyme activities. Independent action model showed antagonistic effect for lower concentrations of n-TiO2 and additive effect for higher concentrations of n-TiO2 when present in mixture with TC under both illuminations. For the higher mixture concentration of 4 mg L-1 n-TiO2 and 1 mg L-1 TC, the percentage TC removal was about 55.29% and 30% and the corresponding TOC removal was found to be 54.29% and 31.04% under visible and UV-A illuminations respectively. The site of ROS generation in Chlorella sp. was identified with electron transfer chain inhibitors. Both mitochondria and chloroplast acted as the site for the ROS generation in Chlorella sp. The SEM images of the algal cells upon exposure to n-TiO2 and mixture revealed the aggregation of cells and distortion of cell membrane.

Influence of differently functionalized polystyrene microplastics on the toxic effects of P25 TiO2 NPs towards marine algae Chlorella sp.

Increased utilization of titanium dioxide nanoparticles (TiO2 NPs) for commercial as well as industrial purposes resulted in the accumulation of nanoparticles in the marine system. Microplastics being an emerging secondary pollutant in the marine ecosystem have an impact on the toxic effects of TiO2 NPs which has not been evaluated up to date. So it is important to assess the toxic effects of both these pollutants on the marine environment. The present study examines the impact of differently functionalized microplastics on the toxic effects of P25 TiO2 NPs to marine algae Chlorella sp. The tendency of nanoparticles to undergo aggregation in artificial seawater was observed with increase in time. The median effective concentration for TiO2 NPs was found to be 81 µM which indicates higher toxic effects of NPs toward algae. In contrast, microplastics irrespective of their difference in functionalization had minimal toxic effect of about 15% at their higher concentration tested, 1000 mg L-1. Plain and aminated polystyrene microplastics enhanced the TiO2 NPs toxicity which was further validated with oxidative stress determination studies like reactive oxygen species and lipid peroxidation assays. Negatively charged carboxylated polystyrene microplastics decreased the TiO2 NPs toxicity with possible hetero-aggregation between TiO2 NPs and microplastics in the system. The toxicity data obtained for the mixture was further corroborated with Abbott's mathematical model.

Dietary transfer of zinc oxide particles from algae (Scenedesmus obliquus) to daphnia (Ceriodaphnia dubia).

The rapid increase in production and usage of ZnO particles in recent years has instigated the concerns regarding their plausible effects on the environment. Current study explores the trophic transfer potential of ZnO particles of different sizes (50, 100 nm and bulk particles) from algae (Scenedesmus obliquus) to daphnia (Ceriodaphnia dubia) and the contribution of ZnO(ions) (effect of dissolved Zn ions that remain in test medium after separation NPs) to the overall toxicity of ZnO(total) (impact of both particle and dissolved Zn ions). Toxicity and uptake of ZnO(total) and ZnO(ions) in algae were found to be dependent on the concentration and particle size. Feeding of Zn accumulated algae (517 ±â€¯28, 354.7 ±â€¯61 and 291 ±â€¯20 µg/g dry wt.) post-exposure to 61 µM of ZnO(total) of 50, 100 nm and bulk ZnO particles caused a significant decrease in the survival (15-20%) of daphnia. A significant amount of Zn accumulation was observed in daphnia even after the 48 h depuration period. Biomagnification factor was found to be nearly 1 for all the sizes of ZnO particles tested. For 50 nm ZnO, the BMF was higher when compared to other two sizes, reaching the mean value of 1.06 ±â€¯0.01 at 61 µM. Further analysis revealed that the dietary uptake of different sizes of ZnO particles caused ultra-structural damages and degradation of internal organs in daphnia.