Omics analysis of 'Shine Muscat' grape grafted on different rootstocks in response to cadmium stress.

Cadmium (Cd) is detrimental to grape growth, development, and fruit quality. Grafting is considered to be a useful method to improve plant adaptability to Cd stress in grape production. However, little information is available on how Cd stress affects grafted grapes. In this study, the effects of Cd on Shine Muscat grapes (Vitis vinifera L. cv. 'Shine Muscat') were studied under different "Cd treatments" concentrations (0, 0.2, 0.4, 0.8, 1.6, 3.2 mg kg-1) and "rootstock treatments" (SO4, 5BB, and 3309C). The results showed that low levels of Cd had hormesis effect and activated the grape antioxidant system to eliminate the ROS induced by Cd stress. The antioxidant capacity of the SM/3309C rootstock combination was stronger than that of the other two groups under low-concentration Cd stress. Moreover, the rootstock effectively sequestered a substantial amount of Cd, consequently mitigating the upward translocation of Cd to the aboveground portions. Transcriptomic and metabolomic analysis revealed several important pathways enriched in ABC transporters, flavonoid biosynthesis, Plant hormone signal transduction, phenylpropanoid biosynthesis, and glutathione metabolism under Cd stress. WGCNA analysis identified a hub gene, R2R3-MYB15, which could promote the expression of several genes (PAL, 4CL, CYP73A, ST, CHS, and COMT), and alleviate the damage caused by Cd toxicity. These findings might shed light on the mechanism of hormesis triggered by low Cd stress in grapes at the transcriptional and metabolic levels.

The Effect of "Production during Remediation" of Plants in Cd-Contaminated Soil.

In order to find suitable plants for "production during remediation" in wheat fields moderately contaminated by cadmium (Cd), five plants-green amaranth, oil sunflower, broomcorn, maize, and waxy maize-were planted in pots to study their enrichment characteristics and remediation effects in Cd-contaminated soil. The results showed that the highest bioaccumulation and translocation factors were greater than 0.5 for oil sunflower, which had the strongest Cd-enrichment ability in Cd-contaminated soil, but its biomass was small, and the Cd content of the grain exceeded the standard (GB2762-2017). The Cd content in the grains of broomcorn, maize, and waxy maize was less than 0.1 mg∙kg-1, which is lower than the national food safety standard on limiting pollutants in food (GB2762-2017). Broomcorn accumulated 0.429 mg∙pot-1 for Cd, with a Cd-extraction efficiency of 1.73%, which were higher than other plants. Taking the risk-screening values in GB15618-2018 "Soil Environmental Quality Standard" as the target, it will take 80 years to remediate using broomcorn, which has the highest extraction efficiency, based on cultivating remediation plants once per year. However, in view of the scarcity of arable land resources in China and the objective of safe production during remediation, the use of broomcorn can be considered for production during remediation for the given degree of Cd contamination of the soil.

Biodegradation of gentamicin by bacterial consortia AMQD4 in synthetic medium and raw gentamicin sewage.

Gentamicin, a broad spectrum antibiotic of the aminoglycoside class, is widely used for disease prevention of human beings as well as animals. Nowadays the environmental issue caused by the disposal of wastes containing gentamicin attracts increasing attention. In this study, a gentamicin degrading bacterial consortia named AMQD4, including Providencia vermicola, Brevundimonas diminuta, Alcaligenes sp. and Acinetobacter, was isolated from biosolids produced during gentamicin production for the removal of gentamicin in the environment. The component and structure of gentamicin have a great influence on its degradation and gentamicin C1a and gentamicin C2a were more prone to being degraded. AMQD4 could maintain relatively high gentamicin removal efficiency under a wide range of pH, especially in an alkaline condition. In addition, AMQD4 could remove 56.8% and 47.7% of gentamicin in unsterilized and sterilized sewage in a lab-scale experiment, respectively. And among the isolates in AMQD4, Brevundimonas diminuta BZC3 performed the highest gentamicin degradation about 50%. It was speculated that aac3iia was the gentamicin degradation gene and the main degradation product was 3'-acetylgentamicin. Our results suggest that AMQD4 and Brevundimonas diminuta BZC3 could be important candidates to the list of superior microbes for bioremediation of antibiotic pollution.

Gentamicin removal in submerged fermentation using the novel fungal strain Aspergillus terreus FZC3.

Social concern and awareness of the potential risk posed by environmental residues of antibiotics such as gentamicin in the development of antibiotic resistance genes have increased. The present study used laboratory-scale experiments to develop methods for gentamicin removal from the environment. A fungus, strain FZC3, which could remove gentamicin in submerged fermentation, was isolated from solid waste and sewage water from a gentamicin production factory. The fungus was identified as Aspergillus terreus by sequencing the PCR-amplified ITS fragments of its rRNA-coding genes and by its morphology. The gentamicin removal efficiency exceeded 95% by day 7 under optimized culture conditions. The results showed that both biosorption and biodegradation were involved. We speculated that Aspergillus terreus FZC3 absorbed gentamicin and subsequently degraded it. We also found that Aspergillus terreus FZC3 survived and maintained a high bioremediation efficiency over a wide pH range, indicating its potential for future use in the large-scale bioremediation of gentamicin.

[In-situ remediation of polluted water body by planting hydrophytes].

In an experimental enclosure system, floating plant Eichhornia crassipes was planted in summer and submersed plant Elodea nutalli was planted in winter to reestablish water ecosystem, and to investigate the effects of the hydrophytes on the removal of nitrogen and phosphorus from polluted water body and the improvement of water transparency. The results showed that compared with the control and native pond water, the water body planted with hydrophytes had a low level of nutrients. E. crassipes had a fast growth, with its covered area increased from 100 m2 to 470 m2 in the first 15 days, and to 65% of the water area after 44 days. The total nitrogen, ammonium nitrogen, nitrite nitrogen, CODMn and chlorophyll a decreased to a lower level, and the water transparency increased significantly, reaching to a depth of 1.7-1.8 m (i.e., to the bottom of the pond). After October, the total phosphorus kept on about 0.1 mg L(-1). Due to the increased water transparency, E. nutalli became the dominant species and covered 1/3 of the water area, playing an important role in purifying water quality, keeping water physicochemical properties in good status, and improving water transparency. It was concluded that planting hydrophytes in polluted water body could efficiently reduce its nutrients level and control the overgrowth of algae, being an important way in improving the water quality of eutrophicated water body.

In-situ nitrogen removal from the eutrophic water by microbial-plant integrated system.

OBJECTIVE: This study was to assess the influence of interaction of combination of immobilized nitrogen cycling bacteria (INCB) with aquatic macrophytes on nitrogen removal from the eutrophic waterbody, and to get insight into different mechanisms involved in nitrogen removal. METHODS: The aquatic macrophytes used include Eichhornia crassipes (summer-autumn floating macrophyte), Elodea nuttallii (winter-growing submerged macrophyte), and nitrogen cycling bacteria including ammonifying, nitrosating, nitrifying and denitrifying bacteria isolated from Taihu Lake. The immobilization carriers materials were made from hydrophilic monomers 2-hydroxyethyl acrylate (HEA) and hydrophobic 2-hydroxyethyl methylacrylate (HEMA). Two experiments were conducted to evaluate the roles of macrophytes combined with INCB on nitrogen removal from eutrophic water during different seasons. RESULTS: Eichhornia crassipes and Elodea nuttallii had different potentials in purification of eutrophic water. Floating macrophyte+bacteria (INCB) performed best in improving water quality (during the first experiment) and decreased total nitrogen (TN) by 70.2%, nitrite and ammonium by 92.2% and 50.9%, respectively, during the experimental period, when water transparency increased from 0.5 m to 1.8 m. When INCB was inoculated into the floating macrophyte system, the populations of nitrosating, nitrifying, and denitrifying bacteria increased by 1 to 2 orders of magnitude compared to the un-inoculated treatments, but ammonifying bacteria showed no obvious difference between different treatments. Lower values of chlorophyll a, COD(Mn), and pH were found in the microbial-plant integrated system, as compared to the control. Highest reduction in N was noted during the treatment with submerged macrophyte+INCB, being 26.1% for TN, 85.2% for nitrite, and 85.2% for ammonium at the end of 2nd experiment. And in the treatment, the populations of ammonifying, nitrosating, nitrifying, and denitrifying bacteria increased by 1 to 3 orders of magnitude, as compared to the un-inoculated treatments. Similar to the first experiment, higher water transparency and lower values of chlorophyll a, COD(Mn) and pH were observed in the plant+ INCB integrated system, as compared to other treatments. These results indicated that plant-microbe interaction showed beneficial effects on N removal from the eutrophic waterbody.