RESUMEN
The application of biphasic calcium phosphate (BCP) in tissue engineering and regenerative medicine has been widely explored due to its extensively documented multi-functionality. The present study attempts to synthesize a new type of BCP nanoparticles, characterised with favourable cytocompatibility and antibacterial properties via modifications in their structure, functionality and assemblage, using dopants. In this regard, this study initially synthesized iron-doped BCP (FB) nanoparticles with silver subsequently incorporated into FB nanoparticles to create a nanostructured composite (FBAg). The FB and FBAgnanoparticles were then characterized using Fourier transform infrared spectroscopy, x-ray diffraction, ultraviolet-visible spectroscopy, and x-ray photoelectron spectroscopy. The results showed that silver was present in the FBAgnanoparticles, with a positive correlation observed between increasing AgNO3concentrations and increasing shape irregularity and reduced particle size distribution. Additionally, cell culture tests revealed that both FB and FBAgnanoparticles were compatible with bone marrow-derived mesenchymal stem cells (hBMSCs). The antibacterial activity of the FBAgnanoparticles was also tested using Gram-negativeE. coliand Gram-positiveS. aureus, and was found to be effective against both bacteria. The inhibition rates of FBAgnanoparticles againstE. coliandS. aureuswere 33.78 ± 1.69-59.03 ± 2.95%, and 68.48 ± 4.11-89.09 ± 5.35%, respectively. These findings suggest that the FBAgnanoparticles have potential use in future biomedical applications.
RESUMEN
Repair of osteochondral defects and regeneration of cartilage is a major challenge. In this work, the mesenchymal stem cells (MSCs)-laden hydrogel was designed using silk fibroin (SF) and gelatin methacrylate (GelMA), to encapsulate platelet-rich plasma (PRP). Initially, GelMA was synthesized, and SF was prepared using silkworm cocoon, then MSCs-laden SF/GelMA (SG) hydrogel was fabricated. The physicochemical properties of the hydrogels were evaluated using Fourier-transform infrared spectroscopy, scanning electron microscope, and rheometry. After hydrogel preparation, the viability of MSCs in the hydrogels was investigated via CCK-8 analysis and fluorescent images. The MSCs-laden SG hydrogel containing PRP was subsequently injected into the cartilage defect area in Sprague Dawley rats. Hematoxylin and eosin (H&E), Masson staining, and Mankin scores evaluation confirmed the new cartilage formation in 8 weeks. The results presented in the study, therefore, showed that the prepared MSCs-laden SG hydrogel loaded with PRP has the potential for cartilage reconstruction, which is crucial to the treatment of knee osteoarthritis.
RESUMEN
Biofilm infections pose a rising threat to public health due to its existing protective shield, which preventing biocide penetration. Here, the oleate-capped iron oxide nanoparticles (OIONPs) were synthesized by the high-temperature method first; after then, the poly(acrylic acid)-capped iron oxide nanoparticles (PIONPs) were obtained via a ligand exchange reaction between OIONPs and sodium poly(acrylic acid). The physicochemical properties of PIONPs were evaluated by Fourier-transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Scanning Transmission Electron Microscopy (STEM), Dynamic Light Scattering (DLS), and zeta potential. The FT-IR analysis confirmed the successful ligand exchange on the surface of iron oxide nanoparticles. STEM images displayed that the prepared PIONPs were monodisperse spherical nanoparticles. The PIONPs were stable in ultrapure water and could be kept for 5 weeks without aggregation. Next, Cell Counting Kit-8 (CCK-8) assay and fluorescent images confirmed the excellent cytocompatibility of PIONPs, while the iron concentration of PIONPs was in the range of 5â¼120 mg/L. Finally, PIONPs exhibited efficient antibacterial activity against E. coli and S. aureus, and Staphylococcus aureus subsp. aureus Rosenbach (SASAR) biofilm could be destroyed by treating PIONPs under alternating current (AC) applied field conditions.
