RESUMEN
Extrahepatic cancers of the biliary system are typically asymptomatic until after metastasis, which contributes to their poor prognosis. Here we examined intrahepatic cholangiocarcinomas (n = 8), carcinomas of perihilar bile ducts (n = 7), carcinomas of the gallbladder (n = 11) and hepatic metastasis from carcinomas of the gallbladder (n = 4) for the expression of the extracellular matrix glycoproteins tenascin-C and tenascin-W. Anti-tenascin-C and anti-tenascin-W immunoreactivity was found in all biliary tract tumors examined. Unlike tenascin-C, tenascin-W was not detected in normal hepatobiliary tissue. Tenascin-W was also expressed by the cholangiocarcinoma-derived cell line Huh-28. However, co-culture of Huh-28 cells with immortalized bone marrow-derived stromal cells was necessary for the formation and organization of tenascin-W fibrils in vitro. Our results indicate that tenascin-W may be a novel marker of hepatobiliary tumor stroma, and its absence from many normal tissues suggests that it may be a potential target for biotherapies.
Asunto(s)
Neoplasias del Sistema Biliar/inmunología , Biomarcadores de Tumor/inmunología , Proteínas de Neoplasias/inmunología , Tenascina/inmunología , Neoplasias del Sistema Biliar/patología , Línea Celular Tumoral , Humanos , Células del Estroma/inmunología , Células del Estroma/patologíaAsunto(s)
Infectología/normas , Osteomielitis/diagnóstico , Osteomielitis/terapia , Enfermedades de la Columna Vertebral/diagnóstico , Enfermedades de la Columna Vertebral/terapia , Humanos , Infectología/métodos , Infectología/organización & administración , Ortopedia/métodos , Ortopedia/organización & administración , Ortopedia/normas , Osteomielitis/epidemiología , Enfermedades de la Columna Vertebral/epidemiología , Túnez/epidemiologíaRESUMEN
Behcet's disease is a vasculitis affecting predominantly the venous system. It's characterized by a classical triad of recurrent uveitis, oral and genital ulceration. Behcet's disease is more frequent in Japan, the Middle East and some Mediterranean countries. Its pathogenesis is still uncertain. Neurological manifestations in Behcet's disease are not rare and are associated with poor prognosis. These manifestations can be inaugural in 5% of cases and they are polymorphous. We report 15 new cases of neuro Behcet's disease explored by magnetic resonance imaging (MRI) and magnetic resonance angiography (MRA).
