Electrosprayed cefazolin-loaded niosomes onto electrospun chitosan nanofibrous membrane for wound healing applications.

Chronic wounds are among the most therapeutically challenging conditions, which are commonly followed by bacterial infection. The ideal approach to treat such injuries are synergistic infection therapy and skin tissue regeneration. In the recent decades, nanotechnology has played a critical role in eradicating bacterial infections by introducing several carriers developed for drug delivery. Moreover, advances in tissue engineering have resulted in new drug delivery systems that can improve the skin regeneration rate and quality. In this study, cefazolin-loaded niosomes were electrosprayed onto chitosan membrane for wound healing applications. For this purpose, niosomes were obtained by the thin-film hydration method; electrospinning was then conducted to fabricate nanofibrous mats. In vitro characterization of the scaffold was performed to evaluate the physicochemical and biological properties. Finally, in vivo studies were carried out to evaluate the potential use of the membrane for skin regeneration. In vitro results indicated the antibacterial properties of the membrane against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) due to the gradual release of cefazolin from niosomes. The scaffolds also showed no cell toxicity. In vivo studies also confirmed the ability of the membrane to enhance skin regeneration by improving re-epithelialization, tissue remodeling, and angiogenesis. The current study could well show the promising role of the prepared scaffold for skin regeneration and bacterial infection elimination.

A high school-based education concerning drug abuse prevention.

OBJECTIVE: There is increasing evidence for declining the onset age of drug abuse worldwide. This study was conducted to investigate the effectiveness of four educational methods including lecture, presentation of poster and leaflet, presentation of video clip, and group/class discussion for life skills training and changing in knowledge and attitude of adolescents toward drug abuse. MATERIALS AND METHODS: In a pretest-posttest design, a sample of 897 girl and boy high school students from the first grade (14-15 years old) were involved in this cross-sectional study conducted in Isfahan, Iran. After collection of pretest questionnaires, each educational method was implemented separately for one class in one session (3 h) in each high school, and evaluation was carried out immediately after intervention through posttest questionnaires by the same students. RESULTS: According to paired t-test, the video clip- and lecture-based methods were significantly efficient in changing the attitudes toward drug abuse in boy and girl students, respectively. Analysis of covariance showed significant differences between girls and boys in pretest-posttest attitude scores using group discussion-based and video clip-based methods. CONCLUSION: Life skills training program through lecture-based and video clip-based educational methods was considerably effective in changing the high school students' attitude toward drug abuse and addiction.