[Cell-free Pseudomonas vaccine. III. Immunochemical analysis of purified Pseudomonas aeruginosa protein antigens and protective properties of antisera against these antigens]. / Beskletochnaia psevdomonas-vaktsina. Soobshchenie III. Immunokhimicheskii analiz ochishchennykh belkovykh antigenov Pseudomonas aeruginosa i protektivnye svoistva antisyvorotok k étim antigenam.

The immunochemical analysis of isolated and purified antigens A and B obtained from P. aeruginosa, strains 868 (serogroup O3 according to Lányi or immunotype 3/7 according to Fisher) and 170015 (serogroup O7 or immunotype 2), was carried out. Rabbit antisera to proteins A and B, as well as to the initial aqueous extracts and partially purified aqueous extracts, were obtained. Cross activity between the protein antigens of different strains was established by the methods of immunodiffusion and two-dimensional immunoelectrophoresis. Isolated proteins A and B contained both common and specific antigenic determinants detected by the method of two-dimensional immunoelectrophoresis. The immunization of rabbits with proteins A and B was found to stimulate the synthesis of protective, probably species-specific, antibodies.

[Natural antibodies against Pseudomonas aeruginosa in human gamma globulin preparations]. / Estestvennye anttela protiv Pseudomonas aeruginosa v preparatakh gamma-globulina cheloveka.

A total of 191 batches of commercial human gamma globulin were studied with the aim of detecting natural antibodies by means of cross and countercurrent electrophoresis, immunodiffusion in agar gel and the passive hemagglutination test. Commercial human gamma globulin preparations were found to contain, to a greater or lesser extent, natural antibodies to the cell-wall O-antigen of Ps. aeruginosa and the antigens of its extracellular slime (capsule); the titers of these antibodies were 1 : 64 to 1 : 256 in 75-85% of cases, 1 : 512 and greater in 10-11% of cases. Commercial gamma globulin preparations also contained antibodies protecting mice from experimental Pseudomonas infection. No correlation was detected between the degree of serological activity and the protective activity of gamma globulin preparations.

[Immunochemical analysis of water-soluble antigen complexes isolated from typing strains of Pseudomonas aeruginosa of different O-serogroups]. / Immunokhimicheskii analiz vodorastvorimykh antigennykh kompleksov, izolirovannykh iz tipovykh shtammov Pseudomonas aeruginosa raznykh O-serogrupp.

Cross immunoelectrophoresis in agarose and immunodiffusion in agar gel were used to carry out the immunochemical analysis of water-soluble antigenic components isolated from P. aeruginosa of different O-serogroups (according to Lanyi's classification). Immunodiffusion revealed the presence of 1--3 common antigens and 1 specific O-antigen in aqueous extracts. Experiments with the use of cross immunoelectrophoresis indicated that 1--12 common antigens could be detected in aqueous extracts. The reference preparation, produced on the basis of the cell mixture of 9 P. aeruginosa strains, contained up to 47 antigenic components, many of them being common to the strains of different O-serogroups (immunotypes).