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Background: Lung adenocarcinoma (LUAD) is the most common subtype of non-small cell lung cancer. Fatty acid metabolism takes part in malignancy progression. However, the roles fatty acid metabolism plays in LUAD are still unclear. Methods: The transcriptomic and clinical data of LUAD patients from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were extracted. ssGSEA, WGCNA, univariable Cox regression, and LASSO Cox regression analyses were performed to identify the fatty acid metabolism-related genes which influenced the overall survival (OS) and build a fatty acid-related risk score (FARS) model. A nomogram was established based on the FARS and other clinicopathological features, and ROC and calibration plots were used to validate the prediction accuracy. The tumor microenvironment (TME) of patients with high and low FARS was compared. Results: A total of 38 genes were identified to be independently related to the survival outcome and put into a FARS model. High FARS patients exhibited significantly worse OS. The nomogram included the FARS and pathological stage, and the AUC of the nomogram predicting 1-, 2-, 3-, 4-, and 5-year OS was 0.789, 0.807, 0.798, 0.809, and 0.753, respectively. Calibration plots also indicated good accuracy. Moreover, the samples of the high FARS had higher expression of PDL1. Conclusion: We constructed a FARS model which could accurately predict the survival outcome of the LUAD patients. The genes of the FARS are related to the tumor microenvironment and patients with high FARS can potentially benefit more from anti-PD1/PDL1 immunotherapy. In addition, the mechanisms of the genes in the FARS affecting prognosis are worthy of further research to develop new gene-targeted drugs.
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ABSTRACT Introduction: The knee joint is a complex and important joint in the human body. It plays an active role in maintaining posture balance of the human body in sports, allowing one to bear a larger load and complete various complex technical movements. Objective: To investigate the stability and influence of kinemechanics in knee joint rehabilitation training. Methods: A total of 80 patients with anterior cruciate ligament injury who received treatment in Henan Provincial People's Hospital were selected and divided into control group and observation group according to the random number table method, with 40 cases in each group. Results: Six months after surgery, the peak torque (PT), peak torque to body weight ratio (PT/BWT), total work (TWT), Lysholm score and Tegner score, and knee circumference of the two groups were significantly improved compared with those before surgery (P <0.01). The degree of improvement was greater in the observation group:compared with the control group, the difference was highly statistically significant (P<0.01). Conclusions: Professional exercise rehabilitation has a definite effect on patients after anterior cruciate ligament reconstruction. It can significantly improve the muscle strength of quadriceps and hamstring, improve the function of knee joint and promote its recovery. Level of evidence II; Therapeutic studies - investigation of treatment results.
RESUMO Introdução: A junta do joelho é uma junta complexa e importante para o corpo humano. Tem um papel ativo na manutenção do equilíbrio postural do corpo humano nos esportes, permitindo que se suporte cargas maiores e que se realize vários movimentos técnicos complexos. Objetivo: Investigar a estabilidade e influência da cinemática no treino para reabilitação da junta do joelho. Métodos: Um total de 80 pacientes com lesão de ligamento cruzado anterior que receberam tratamento no Hospital do Povo da Província de Henan foram selecionados e divididos em grupo de controle e grupo de observação, de acordo com o método de tabela numérica aleatória, com 40 casos em cada grupo. Resultados: Seis meses após a cirurgia, o torque de pico (TP), a proporção torque de pico-peso corporal (TP-PC), trabalho total (TT), escore Lysholm e escore Tegner, e a circunferência do joelho dos dois grupos melhoraram significativamente em relação à antes da cirurgia (P <0.01). O grau de recuperação foi maior no grupo de observação: comparado com o grupo de controle, a diferença é estatisticamente significativa (P <0.01). Conclusões: A reabilitação com exercícios profissionais definitivamente tem efeito em pacientes após a reconstrução do ligamento cruzado anterior, pois pode aumentar a força muscular de quadríceps e tendões, melhorar a função da junta do joelho e levar a sua recuperação. Nível de evidência II; estudos terapêuticos - investigação de resultados de tratamento.
RESUMEN Introducción: La articulación de la rodilla es compleja e importante para el cuerpo humano. Tiene un papel activo en la manutención del equilibrio postural del cuerpo humano en los deportes, permitiendo que se soporte cargas mayores y que se realice varios movimientos técnicos complejos. Objetivo: Investigar la estabilidad e influencia de la cinemática en el entrenamiento para rehabilitación de la articulación de la rodilla. Métodos: Se seleccionó un total de 80 pacientes con lesión de ligamento cruzado anterior que recibieron tratamiento en el Hospital del Pueblo de la Provincia de Henan y se los dividió en grupo de control y grupo de observación, de acuerdo con el método de tabla de números aleatorios, con 40 casos en cada grupo. Resultados: Seis meses tras la cirugía, el torque de pico (TP), la proporción torque de pico-peso corporal (TP-PC), trabajo total (TT), puntuación Lysholm y puntuación Tegner, y la circunferencia de la rodilla de los dos grupos mejoraron significativamente en relación a antes de la cirugía (P<0.01). El grado de recuperación fue mayor en el grupo de observación: comparado con el grupo de control, la diferencia es estadísticamente significativa (P<0.01). Conclusiones: La rehabilitación con ejercicios profesionales definitivamente tiene efecto en pacientes tras la reconstrucción del ligamento cruzado anterior, pues puede aumentar la fuerza muscular de cuádriceps y tendones, mejorar la función de la articulación de la rodilla y llevar a su recuperación. Nivel de evidencia II; Estudios terapéuticos - investigación de resultados de tratamiento.
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Purpose: To develop and validate a gene-related nomogram for predicting the risk of lymph node (LN) metastasis preoperatively in patients with colorectal cancer (CRC). Methods: RNA-seq data of 581 CRC and 51 normal cases with clinical features were downloaded from TCGA database. In the evaluation cohort with 381 CRC cases, the LASSO regression was used to reduce dimensionality of gene signatures extracted to build gene score. A gene-related nomogram was performed based on the multivariable logistic regression analysis. The performance of the nomogram was assessed by the discrimination, calibration, and clinical usefulness not only in the evaluation, but also in the validation cohort with 200 CRC cases. Results: A total of 12,590 differentially expressed genes were selected, in which 59 candidates associated with LN metastasis in differentially expressed genes set were screened by LASSO to form the gene score. Based on the analysis of multivariate logistic regression, the gene-related nomogram showed good calibration and discrimination not only in the evaluation cohort (concordance-index 0.93; 95%CI 0.91-0.96), but also in the validation cohort (concordance-index 0.70; 95%CI 0.63-0.78). The decision curve analysis of the gene-related nomogram also provides constructive guidance for the design of operation plan, preoperatively. Conclusions: The presented genes nomogram may predict the LN metastasis in CRC patients, preoperatively. And 59 hub genes were defined related to LN metastasis of CRC, which can serve as treatment targets for the further study. Preoperative biopsy and gene analysis are needed to develop the operation plan in clinical practice.
