Discrimination of untreated and sodium sulphite treated bean sprouts by Fourier transform infrared spectroscopy and chemometrics.

Sprouts of black beans (Phaseolus vulgaris L.), soybeans (Glycine max L.) and mung beans (Vigna radiata L.) are widely consumed foods containing abundant nutrients with biological activities. They are commonly treated with sulphites for the preservation and extension of shelf-life. However, our previous investigation found that immersing the bean sprouts in sulphite might convert the active components into sulphur-containing derivatives, which can affect both the quality and safety of the sprouts. This study explores the use of FTIR in conjunction with chemometric techniques to differentiate between non-immersed (NI) and sodium sulphite immersed (SI) black bean, soybean and mung bean sprouts. A total of 168 batches of raw spectra were obtained from NI and SI-bean sprouts using FTIR spectroscopy. Four pre-processing techniques, three modelling assessment techniques and four model evaluation indices were examined for differences in performance. The results show that the multiplicative scatter correction is the most effective pre-processing method. Among the models, the accuracy rate of the three models was as follows: radial basis function neural network (95%) > convolutional neural network (91%) > random forest (82%). The overall findings indicate that FTIR spectroscopy, in conjunction with appropriate chemometric approaches, has a high potential for rapidly determining the difference between NI and SI-bean sprouts.

Age of acquisition effects in Chinese two-character compound words: a megastudy of eye movements during reading.

Words learned earlier in life are processed faster than words learned later in life. In Chinese, the age of acquisition (AoA) effect has been extensively studied in one-character words, yet no studies have explored this effect in compound words. Given that compound words are a widespread word type and are processed differently than one-character words, it is unclear how AoA affects compound word processing. The present study examined the role of multiple variables in the processing of Chinese two-character compound words based on a database of eye-movement measures. We focused on the AoA effect from the whole compound word and the first and second characters. We found that whole-word AoA and first-character AoA have an early and long-lasting influence, with no AoA effect from the second character. The results indicated that two-character compound words are accessed via the character and whole word simultaneously. These findings are discussed with respect to theories of the AoA effect.

Antitumor Activity and Mechanism of Terpenoids in Seaweeds Based on Literature Review and Network Pharmacology.

Seaweeds are a treasure trove of natural secondary metabolites. Terpenoids extracted from seaweeds are shown to possess a variety of antitumor cellular activities. However, due to the complex and diverse structures of terpenoids, their therapeutic targets and complex mechanisms of action have not been clarified. The present study summarises the research on terpenoids from seaweeds in oncological diseases over the last 20 years. Terpenoids show different degrees of inhibitory effects on different types of tumor cells, suggesting that terpenoids in seaweeds may have potential antitumor disease potential. Terpenoids with potential antitumor activity and their mechanism of action are investigated using network pharmacology. A total of 125 terpenoids and 286 targets are obtained. Proto-oncogene tyrosine-protein kinase Src(SRC), Signal transducer and activator of transcription 3 (STAT3), Mitogen-activated protein kinase (MAPK3, MAPK1), Heat shock protein HSP 90-alpha (HSP90AA1), Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), and RAC-alpha serine/threonine-protein kinase (AKT1) are defined as core targets. According to GO function and Kyoto encyclopedia of genes and genomes(KEGG) enrichment analysis, terpenoids may affect the Phoshatidylinositol 3'-kinase (PI3K)-Akt signaling pathway, Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor resistance, Prostate cancer, MAPK signaling pathway, and Proteoglycans in cancer. In addition, the molecular docking results show that the selected terpenoids are all able to bind strongly to the active protein. Terpenoids may slow down the progression of cancer by controlling apoptosis, proliferation, and protein and enzyme binding.

Basic and clinical study of the effect of exogenous hyaluronic acid on the quality of acellular dermal matrix combined with thin intermediate split thickness skin graft.

BACKGROUND: To promote wound recovery in the recipient region, we studied the impact of exogenous hyaluronic acid (HA) on acellular dermal matrix (ADM) paired with thin intermediate-thickness skin transplant. METHODS: This study contains animal and clinical experiments. 50 Japanese big ear rabbits were separated into HA1, HA2, PADM, TS, and NS groups. Clinical part included 50 scar patients dividing into 5 groups (TS + HA + ADM 1, TS + ADM2, TS, TS + ADM and normal skin (NS)). RESULTS: In the animal trial, after 56 days, the grafts contracted least in the HA2 group; HA2 had the highest microvascular density (MVD), HA concentration, and collagen I and III expression. In clinical work, ADM > HA + ADM2 > HA + ADM1 > TS > NS; Type I and III collagen: HA + ADM1 and HA + ADM2 were higher than ADM; HA content: TS > HA + ADM1 > HA + ADM 2 > ADM. CONCLUSIONS: ADM, exogenous hyaluronic acid mixed with thin skin autograft has better biomechanical qualities and therapeutic impact than acellular dermal matrix alone, and the reconstructive result is near to self-thick skin autograft in all indexes.

Ultrapulse Fractional CO 2 Laser With Different Fluences and Densities in the Prevention of Periorbital Laceration Scars: A Split-Scar, Evaluator-Blinded Study.

BACKGROUND: Periorbital laceration can result in complex, permanent scars, and even lead to serious complications such as cicatricial ectropion. Early intervention with laser devices has been suggested as a novel modality to reduce scar formation. However, no consensus exists regarding the optimal treatment parameters for scar management. This study evaluated the efficacy and safety of ultrapulse fractional CO 2 laser (UFCL) with different fluences and densities in preventing periorbital surgical scars. OBJECTIVE: To assess the efficacy and safety of UFCL with different fluences and densities in the prevention of periorbital laceration scars. METHODS: A prospective, randomized, blinded study was conducted on 90 patients with periorbital laceration scars of 2 weeks old. Four treatment sessions of UFCL were administered to each half of the scar at 4-week intervals, with halves treated with high fluences with low density versus low fluences with low-density treatment. Vancouver Scar Scale was used to assess the 2 portions of each individual scar at baseline, final treatment, and 6 months. The patient's 4-point satisfaction scale was used to evaluate the patient's satisfaction at baseline and 6 months. Safety was evaluated by registration of adverse events. RESULTS: Eighty-two of 90 patients completed the clinical trial and follow-up. There was no significant difference in Vancouver Scar Scale and satisfaction score between different laser settings between the two groups ( P > 0.05). Adverse events were minor and no long-term side effects were noted. CONCLUSIONS: Early application of UFCL is a safe, strategy to significantly improve the final traumatic periorbital scar appearance. Objective evaluation of scars did not identify differences in scar appearance between high fluences with low density versus low fluences with low density of UFCL treatment. LEVEL OF EVIDENCE: Level III.

Learning new meanings for known L2 words: Long-term semantic representation is updated to integrate new information after consolidation.

Previous research about learning new meanings for known words in second language (L2) has found that semantic relatedness, i.e., congruency, between new and existing meanings benefits encoding and explicit memory of new meanings, and reduces instant interference on accessing existing meanings. However, they did not take the memory consolidation process into account. Thus, integration of new meaning into long-term semantic memory, update of existing meaning representation, and the impact of semantic relatedness between new and existing meanings in this process remain unclear. The present study used the event-related potential (ERP) technique to explore these questions. We asked Chinese students to learn English known words' subdominant meanings variedly related to existing meanings and probed semantic representations with EEG recorded in primed lexical decision tasks four times before and after consolidation. We found that new meaning needs to go through offline consolidation to get integrated. Semantic relatedness/congruency boosted new meaning integration, not by directly expediting it during encoding or preliminary offline consolidation, but by promoting the update of existing meaning representation first, which presumably paved the way for better incorporation of new meaning in the long run. The whole pattern of results implies that long-term semantic representation of existing meaning is updated to integrate related new meaning after consolidation, which not only draws a clearer picture of L2 ambiguous word acquisition but also bears broader implications for research on memory updating.

[Plasma Levels and Clinical Significance of Cytokine Profile in Patients with Multiple Myeloma].

OBJECTIVE: To investigate the levels of cytokines in the plasma of patients with multiple myeloma(MM), and explore its clinical significance. METHODS: The levels of 6 cytokines(IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ) in the plasma of 59 newly diagnosed MM patients and 30 healthy controls were retrospectively analyzed, and the immunophenotypes were also analyzed. The plasma levels of IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ were quantitatively detected by flow microsphere technology, and the differences of cytokines levels in each group were tested by Wilcoxon. RESULTS: The plasma concentration of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ in MM patients were all significantly higher than those in healthy controls (P<0.05). According to the ISS staging, there were no statistically significant difference in cytokines levels of patients at each stage (P>0.05). MM patients with high CD56 expression had higher plasma levels of IL-6 than the CD56 low expression group (41.74±62.73 vs 6.31±5.60 pg/ml) (P<0.05). The plasma level of IL-6 in MM patients with high CD117 expression was higher than that in the CD56 low expression group, but there was no statistically difference (P>0.05). The plasma level of IL-6 in MM patients was significantly decreased after chemotherapy (P<0.05). CONCLUSION: IL-6 is significantly increased in newly diagnosed MM patients, and is associated with the CD56 expression of abnormal plasma cells, which could provide important auxiliary effect on diagnosis of MM; at the same time, it is significantly decreased after chemotherapy, which may be suitable as a monitoring indicator in treatment of MM patients.

The Role of Language Switching During Cross-Talk Between Bilingual Language Control and Domain-General Conflict Monitoring.

The relationship between bilingual language control and executive control is debated. The present study investigated the effect of short-term language switching in a comprehension task on executive control performance in unbalanced bilinguals. Participants were required to perform a context task and an executive control task (i.e., flanker task) in sequence. A picture-word matching task created different language contexts in Experiment 1 (i.e., L1, L2, and dual-language contexts). By modifying the color-shape switching task, we created different contexts that do not involve language processing in Experiment 2 (i.e., color, shape, and dual context). Experiment 1 showed overall faster responses (in both congruent and incongruent trials) in the flanker task after a language switching context than after single (L1 or L2) contexts. This suggests that the language switching in a comprehension task affected general monitoring performance. By contrast, the nonlinguistic contexts in Experiment 2 did not affect flanker performance. This provides further evidence for the crucial role of language processing during switching to elicit short-term adaptions on domain-general conflict monitoring. Overall, our findings add to the previous studies by showing cross-talk between bilingual language control and domain-general conflict monitoring when language switching occurs in a comprehension task.

The letter position coding mechanism of second language words during sentence reading: Evidence from eye movements.

We conducted three eye movement experiments to investigate the mechanism for coding letter positions in a person's second language during sentence reading; we also examined the role of morphology in this process with a more rigorous manipulation. Given that readers obtain information not only from currently fixated words (i.e., the foveal area) but also from upcoming words (i.e., the parafoveal area) to guide their reading, we examined both when the targets were fixated (Exp. 1) and when the targets were seen parafoveally (Exps. 2 and 3). First, we found the classic transposed letter (TL) effect in Exp. 1, but not in Exp. 2 or 3. This implies that flexible letter position coding exists during sentence reading. However, this was limited to words located in the foveal area, suggesting that L2 readers whose L2 proficiency is not as high as skilled native readers are not able to extract and utilise the parafoveal letter identity and position information of a word, whether the word length is long (Exp. 2) or short (Exp. 3). Second, we found morphological information to influence the magnitude of the TL effect in Exp. 1. These results provide new eye movement evidence for the flexibility of L2 letter position coding during sentence reading, as well as the interactions between the different internal representations of words in this process. Future L2 reading frameworks should integrate word recognition and eye movement control models.

The impact of learning new meaning on the previously learned meaning of L2 ambiguous words: The role of semantic similarity.

Previous study has found that previously learned meaning affects the learning of new meaning for known second language (L2) words. However, it is not clear whether learning a new meaning also affects the previously learned meaning and whether this effect is modulated by the semantic similarity between them. The current study aimed to explore this issue using event-related potential technique. A word learning task was used, in which Chinese-English bilinguals were required to learn a new meaning that was semantically related or unrelated to the previously learned meaning of familiar L2 words and judge the semantic relatedness between the trained word and a probe word in the same trial. The results showed that both the N400 and late-positive component (LPC) amplitudes for probe words in the unrelated new meaning condition were significantly different from the unlearned condition, which suggests learning new meaning interferes with accessing the previously learned meaning. Moreover, significantly less positive LPC was found in the unrelated new meaning condition relative to the related new meaning condition, showing a mediation of semantic similarity in the perturbation effect. We conclude that learning L2 new meaning has a backward interference effect on accessing the previously learned meaning and this effect is modulated by semantic similarity. These findings provide supporting evidence for the interaction mechanism of learning the multiple meanings of L2 ambiguous words.

Switching between newly learned languages impacts executive control.

Previous research has explored the relationship between language control and executive control based on performance in bilinguals' skilled languages. However, this relationship between bilingualism and executive control has not been examined at the very initial stage of language learning. In the present study, we trained Chinese speakers to learn words in German and Japanese, two languages with which they had no prior experience. In pre- and post-training, we measured participants' electrophysiological data to investigate how switching between these two newly learned languages affected executive control. We observed that, while lacking the language switching effect in the behavioral data, a flanker task elicited larger N2 and P3 amplitudes in the post-training session when participants were required to switch between German and Japanese compared to when they responded to only German or Japanese. These results provided evidence of language control of newly learned languages on domain-general executive control, specifically at the (very) initial period of language learning. Our findings support the adaptive nature of the relationship between bilingual language control and executive control.

Efficacy and safety of Shen-Ling-Bai-Zhu-San combined with chemotherapy for lung cancer: A protocol for systematic review and meta-analysis.

BACKGROUND: Lung cancer (LC), with the high incidence in malignant tumors in the world, and seriously affects people's lives and brings a great economic burden. Previous clinical studies on Shen-Ling-Bai-Zhu-San (SLBZS) combined with chemotherapy for the treatment of lung cancer have been increasing, but there are no systematic reviews. This study aims to systematically study the efficacy and safety of SLBZS combined with chemotherapy in the treatment of LC. METHODS: The Chinese and English databases will be searched by us for related documents, and the search time limit is January 2021. Databases including PubMed, Embase, Web of Science, the Cochrane Library, Chinese databases include China National Knowledge Infrastructure, Wanfang Data, ChongqingVIP Information Resource Integration Service Platform, China Biomedical Literature. The international clinical trial registration platform and the Chinese clinical trial registration platform will be searched by us to find ongoing or unpublished trials. After screening the literature based on inclusion and exclusion criteria, 2 researchers independently extracted data. The primary outcomes were the treatment efficiency. RevMan 5.3.5 software will be used for statistical analysis. The Recommendation, Assessment, Development, and Evaluation (GRADE) system will be used to evaluate the quality evidence of each result. RESULTS: This study will provide the latest evidence for the SLBZS combined with chemotherapy for LC. CONCLUSION: The efficacy and safety of SLBZS combined with chemotherapy for LC will be evaluated. UNIQUE INPLASY NUMBER: INPLASY202110025.

Classification of Achilles tendon shortening induced by scar contracture and corresponding treatment strategies for pediatric patients - A single unit experience.

BACKGROUND: Achilles tendon shortening of pediatric patients caused by scar contracture poses a challenge for us. It always impairs walking function. In this article, we attempted to introduce a new classification of Achilles tendon shortening of pediatric patients and corresponding treatment strategies in our single center. METHODS: From 2001 to 2018, 65 patients (aging from 13 to 17-years-old, 34 females and 31 males, 21 cases with unilateral Achilles tendon shortening and 44 cases with bilateral Achilles tendon shortening) were recruited. The causes included trauma (n = 13), scald (n = 20) and burn (n = 32). The distance between the heel and the ground was from 3 to 18 cm. They were classified into three types: ≤5 cm, mild, n = 9; 5-10 cm, moderate, n = 30; ≥10 cm, serious, n = 26. They had a history from 7 months to 4 years (28 cases with less than 1.5 years and 37 cases with more than 1.5 years). Treatment methods: Scar-Achilles-Tendon (SAT) flaps and skin graft were used for moderate cases before special external fixation shoes were used for fixation for at least 6 months. External special shoes fixation was used for mild cases except 5cases still received SAT flap and skin graft. In serious cases, bone extraction was used for at least 6 months before receiving SAT flap and skin graft. RESULTS: The distance between the heel and the ground was 0 cm after treatment in 54 cases (mild, n = 9; moderate, n = 28; serious, n = 18). Recurrence was found in 11 cases (mild, n = 0; moderate, n = 5; serious, n = 6) after six months follow-up. There were 13 cases of tangential excision of eschar and 8 cases of escharectomy (P < 0.05) with flap necrosis affection. Among them, 9 cases with a medical history of less than 1.5 years had partial necrosis, 6 cases with a medical history of more than 1.5 years had partial flap necrosis(P < 0.05). Local necrosis was covered by skin graft again. Bone exposure was found in 5 serious cases. It was repaired by negative pressure therapy first and then skin graft was used. The walking ability (P < 0.05) and function (P < 0.05) of lower limbs were statistically improved after treatment. CONCLUSIONS: Different methods can be used according to the shortening degree of Achilles tendon of pediatric patients based on the new classification, which may be useful for future clinical work.

Using rational strategies for children's scars based on more than 5 years assessment after burn.

BACKGROUND: Paediatric patients with scars after burn are regularly encountered. Discussion of rational strategies for management are of value. The objective of the study was to describe development and utilisation of strategies for paediatric burn scars up to five years after injury. METHODS: We included 164 cases aged from 5 to 8 years old in our study; all had burn scar deformities. RESULTS: Assessments were made up to December 31, 2019. The following strategies were used: sequential treatment, reconstruction based on facial aesthetic units, predicting the effect of surgery on development, releasing skin tension and application of photoelectric technology or other non-surgical measures. CONCLUSIONS: Using rational strategies for paediatric burn scars is very important.

A comprehensive reconstruction strategy for moderate to severe faciocervical scar contractures.

The focus of treatment of faciocervical scar contractures includes cervical reconstruction and elimination of hypertrophic scars. Unfortunately, most previous studies have neglected the esthetic appearance of scars. In this study, we tried to combine surgical therapy and ultrapulse fractional CO2 laser (UFCL) to eliminate facial scars while restoring neck reconstruction and to establish the optimal conventional management for faciocervical contracture. Thirty-eight individuals were enrolled and divided into two groups. After received cervical release surgeries, comprehensive UFCL therapy group received treatment of UFCL at 3-month intervals, silicone sheets, and pressure garments, while another group only received treatment of silicone sheeting and compression. Twelve months after the termination of therapy, faciocervical scars of both two groups were assessed by two uninvolved physicians according to the Vancouver Scar Scale (VSS), and patients' satisfaction survey was also recorded by the study participants using a patient four-point satisfaction scale. Thirty-six patients completed the treatment and follow-up. The results show that the VSS scores of both two groups decreased after 12 months, but comprehensive UFCL therapy group dropped more significantly than the conventional treatment group at follow-up session, which was statistically significant (P < 0.001), and the patient satisfaction was higher than that of the conventional treatment group. This comprehensive treatment combined of surgery, UFCL, silicone sheets, and pressure garments works as an effective and esthetic reconstruction for moderate to severe postburn faciocervical scar contractures.

Combination therapy of refractory keloid with ultrapulse fractional carbon dioxide (CO2 ) laser and topical triamcinolone in Asians-long-term prevention of keloid recurrence.

Keloid often recur after treatment, and recent studies in keloid management favor the combination therapy of laser-assisted drug delivery over monotherapy. Unfortunately, the previous researches lack long-term follow-up. In this prospective study, 41 individuals with refractory keloids underwent eight treatment sessions at 4 weeks intervals consisting of ultrapulse fractional carbon dioxide laser (UFCL), followed by postoperative application topical triamcinolone acetonide (40 mg/ml). Four follow-up moments were chosen, with follow-up of 24 months. The effects of combination therapy on scar pliability, thickness, relief, vascularization, surface area, pain, and itchiness were examined by means of the Patient and Observer Scar Assessment Scale (POSAS). 38 patients completed the full 24 months of follow-up after the whole treatment. The results reveal a fast and abiding improvement of keloid scars after the combination therapy. The mean keloid POSAS scores showed a decreasing trend in subsequent times. All POSAS components improved significantly between baseline and 24 months after start of therapy (P < .05). Long-term follow-up results demonstrate that combination keloid therapy using UFCL and tropical triamcinolone has overall significant improvement and low recurrence rate with a long-term stable results.

A Database of Chinese-English Bilingual Speakers: Ratings of the Age of Acquisition and Familiarity.

Recently, considerable attention has been given to the effect of the age of acquisition (AoA) on learning a second language (L2); however, the scarcity of L2 AoA ratings has limited advancements in this field. We presented the ratings of L2 AoA in late, unbalanced Chinese-English bilingual speakers and collected the familiarity of the L2 and the corresponding Chinese translations of English words. In addition, to promote the cross-language comparison and motivate the AoA research on Chinese two-character words, data on AoA, familiarity, and concreteness of the first language (L1) were also collected from Chinese native speakers. We first reported the reliability of each rated variable. Then, we described the validity by the following three steps: the distributions of each rated variable were described, the correlations between these variables were calculated, and regression analyses were run. The results showed that AoA, familiarity, and concreteness were all significant predictors of lexical decision times. The word database can be used by researchers who are interested in AoA, familiarity, and concreteness in both the L1 and L2 of late, unbalanced Chinese-English bilingual speakers. The full database is freely available for research purposes.

The Effect of Semantic Similarity on Learning Ambiguous Words in a Second Language: An Event-Related Potential Study.

Ambiguous words have multiple meanings. How these multiple meanings interact with each other during ambiguous word learning remains unclear. The current study adopted an event-related potentials (ERPs) technique to explore whether there is an interaction between two meanings when learning second language (L2) ambiguous words and how semantic similarity affects ambiguous word learning. In order to explore this issue, Chinese-English bilinguals were asked to learn pseudowords, which were paired with either two related new meanings (polysemes), two unrelated new meanings (homonyms), or one single new meaning (monosomies) over 2 consecutive days. ERP results revealed that learning the second meaning of a homonym induced a more negative N400 than the first meaning; learning the second meaning of a polyseme tended to produce a more positive late component (LPC) than the first meaning. These results indicate that the first meaning of homonyms may interfere with learning their second meaning. However, the first meaning of polysemous words may facilitate learning their second meaning. The current findings suggest that different mechanisms might be involved in learning L2 homonyms and polysemes.

Effects of language context on executive control in unbalanced bilinguals: An ERPs study.

The influence of the language context on language control has been widely discussed in the bilingualism literature, and there is an increase in studies examining the effect of language context on domain-general executive control. However, it remains unclear how language contexts affect executive control performance. In the present study, we created single- and mixed-language comprehension contexts. Unbalanced Chinese-English bilinguals completed a modified flanker task that was interleaved with a single-language or mixed-language picture-word matching task. The effects of language comprehension context on language control were reflected by the N2 and LPC effects. Executive control processes also differed depending on the language comprehension context, with faster behavioral responses and larger N2 but smaller P3 electrophysiological components in the mixed-language context. Moreover, the LPC amplitude in the mixed-language context predicted the behavioral performance in the executive control task. These findings suggested that flexible language control during language comprehension altered executive control processes in unbalanced bilinguals.

HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway.

Acute myelogenous leukemia (AML) is a class of malignant tumors derived from hematopoietic stem or progenitor cells. The H2.0-like homeobox gene (HLX) encodes transcription factors that function in promoting normal hematopoietic cell proliferation and tumor immunity. The present study analyzed the effect of downregulating the HLX on cell cycle distribution and cell proliferation in AML. Moreover, the current study detected changes in the expression of genes and proteins in the Janus kinase (JAK)/STAT signaling pathway to investigate the mechanism of the action of HLX in tumor immunity in AML. HLX expression in AML cell lines was silenced using small interfering siRNA, and MTS/PMS-assay colorimetric assays were used to assess the effect of knockdown of HLX on AML cell proliferation. Flow cytometry was used to analyze changes in cell cycle distribution, while reverse transcription-quantitative PCR and western blotting were used to detect changes in the expression levels of key components of the JAK/STAT signaling pathway, such as p21-activated kinase 1 (PAK1), neuropilin 1 (NRP1), B-cell translocation gene 1 (BTG1) and STAT5. It was found that HLX was differentially expressed in AML cell lines of various subtypes, and HLX expression was higher in the AML/M3 subtype NB4 cell line compared with the control group. Knockdown of HLX in NB4 cells significantly inhibited cell proliferation and arrested cells in the G0/G1 phase. Moreover, STAT5 protein expression, as well as NRP1 and PAK1 expression levels were downregulated, while BTG1 expression was upregulated when HLX was knocked out by siRNA. Collectively, the results suggested that downregulation of HLX may cause G0/G1 phase arrest and inhibit the proliferation of AML cells by activating the JAK/STAT signaling pathway.