1.
Taiwan J Obstet Gynecol
; 52(2): 294-6, 2013 Jun.
Artículo
en Inglés
| MEDLINE
| ID: mdl-23915870
2.
Anal Biochem
; 356(1): 132-41, 2006 Sep 01.
Artículo
en Inglés
| MEDLINE
| ID: mdl-16769031
RESUMEN
A novel, cartridge-based procedure for the efficient and irreversible detritylation of oligonucleotides is reported. This method, combined with a process for the elimination of depurinated fragments produces, in a highly parallel fashion, oligonucleotides with better purity than those traditionally obtained using reversed-phase high-performance liquid chromotography purification. Our combined detritylation and purification methodology compares favorably with commercial cartridge-based purification systems. The benefits of working with pure oligonucleotides, with regard to higher signal and better signal linearity, are shown in array-based hybridization experiments.