Mutation V65I in the ß1 Subunit of the Nicotinic Acetylcholine Receptor Confers Neonicotinoid and Sulfoxaflor Resistance in Insects.

Neonicotinoids have been widely used to control pests with remarkable effectiveness. Excessive insecticides have led to serious insect resistance. Mutations of the nicotinic acetylcholine receptor (nAChR) are one of the reasons for neonicotinoid resistance conferred in various agricultural pests. Two mutations, V65I and V104I, were found in the nAChR ß1 subunit of two neonicotinoid-resistant aphid populations. However, the specific functions of the two mutations remain unclear. In this study, we cloned and identified four nAChR subunits (α1, α2, α8, and ß1) of thrips and found them to be highly homologous to the nAChR subunits of other insects. Subsequently, we successfully expressed two subtypes nAChR (α1/α2/α8/ß1 and α1/α8/ß1) by coinjecting three cofactors for the first time in thrips, and α1/α8/ß1 showed abundant current rapidly. Acetylcholine, neonicotinoids, and sulfoxaflor exhibited different activation capacities for the two subtypes of nAChRs. Finally, V65I was found to significantly reduce the binding ability of nAChR to neonicotinoids and sulfoxaflor through electrophysiology and computer simulations. V104I caused a decrease in agonist affinity (pEC50) but an increase in the efficacy (Imax) of nAChR against neonicotinoids and reduced the binding ability of nAChR to sulfoxaflor. This study provides theoretical and technical support for studying the molecular mechanisms of neonicotinoid resistance in pests.

Accuracy of the Cage Placement in Oblique Lumbar Interbody Fusion and its Effects on the Radiological Outcome in Lumbar Degenerative Disease.

STUDY DESIGN: A retrospective study. OBJECTIVES: This study aimed to check how accurately cages were inserted and how they affected the radiological results in oblique lumbar interbody fusion (OLIF) at L2-L5. METHODS: A total of 137 patients diagnosed with lumbar degenerative disease, 184 intervertebral discs were included. We used a new cage deviation classification system on magnetic resonance imaging (MRI) to determine cage insertion accuracy. Cage deviation angles (CDA) were classified into four groups based on the angle formed by the long axis of the cage and the horizontal axis of the vertebral body. Other radiological parameters on plain radiographs and MRI were compared based on this classification. RESULTS: Among 183 cages, 19 were in zone Ⅰ-Ⅱ (10.32%), 163 were in zone II-III (88.59%), and two were in zone III-IV (1.09%). The median cage deviation was 4.97°. No significant differences (H = 2.479, P = .290 > .05) of CDA were found among different segments. Posterior cage deviation accounted 94.57%. The minimal, mild, moderate, and severe cage deviation was 89 (48.4%), 51 (27.7%), 30 (16.3%), and 14 (7.6%) respectively. No differences in radiological parameter changes were noted among different cage obliquity categories. CONCLUSIONS: Approximately 98.91% of cages were placed in zones I-II and II-III. Most cages deviated posteriorly with CDA ranging minimal to moderate. Minimal to moderate cage deviation did not impact radiological outcomes significantly in OLIF at L2-L5. However, avoiding severe cage deviation is crucial to prevent contralateral traversing nerve root injuries.

Functional diversity of sodium channel variants in common eastern bumblebee, Bombus impatiens.

For the past decade, Colony Collapse Disorder has been reported worldwide. Insecticides containing pyrethroids may be responsible for a decline in bees, which are more sensitive to pyrethroids compared with other insects. Voltage-gated sodium channels (Nav ) are the major target sites of pyrethroids, and the sodium channel diversity is generated through extensive alternative splicing and RNA editing. In this study, we cloned and analyzed the function of variants of the Nav channel, BiNav , from Bombus impatiens. BiNav covers a 46 kb genome region including 30 exons. Sequence analysis of 56 clones showed that the clones can be grouped into 22 splice types with 11 optional exons (exons j, w, p, q, r, b, e, t, l/k, and z). Here, a special alternative exon w is identified, encoding a stretch of 31 amino acid resides in domain I between S3 and S4. RNA editing generates 18 amino acid changes in different positions in individual variants. Among 56 variants examined, only six variants generated sufficient sodium currents for functional characterization in Xenopus oocytes. In the presence of B. impatiens TipE and TEH1, the sodium current amplitude of BiNav 1-1 increased by fourfold, while TipE of other insect species had no effect on the expression. Abundant alternative splicing and RNA editing of BiNav suggests the molecular and functional pharmacology diversity of the Nav channel for bumblebees. This study provides a theoretical basis for the design of insecticides that specifically target pests without affecting beneficial insects.

Comparison between OLIF and MISTLIF in degenerative lumbar stenosis: an age-, sex-, and segment-matched cohort study.

To compare outcomes after oblique lateral interbody fusion (OLIF) versus minimally invasive transforaminal lumbar interbody fusion (MISTLIF) with bilateral decompression via unilateral approach for treating mild to moderate symptomatic degenerative lumbar spinal stenosis (DLSS). We retrospectively compared patients who underwent single-level (L4/5) OLIF with an age-, sex-, and segment-matched MISTLIF with bilateral decompression via unilateral approach cohort. Perioperative data were collected for the operative time, intraoperative blood loss, drainage in the first postoperative day, postoperative hospital stay, cost, intraoperative fluoroscopy, and complications. Lumbar radiographs were measured for changes in posterior intervertebral space height (PISH), intervertebral space foramen height (IFH), intervertebral foramen area (IFA), and area of the spinal canal (ASC). Clinical and psychological outcomes included the visual analog scale (VAS), Oswestry Disability Index (ODI), and hospital anxiety and depression scale (HADS). 35 OLIF patients were compared with 35 MISTLIF patients in L4/5 DLSS. The OLIF group had shorter bedtime, postoperative hospital stays, less intraoperative and postoperative blood loss (all P < 0.05), but had more times of intraoperative fluoroscopy, longer operative time, and higher cost (all P < 0.05). The complication rates were equivalent (OLIF vs MISTLIF: 22.86% vs 17.14%). PISH (11.94 ± 1.78 mm vs 9.42 ± 1.94 mm, P < 0.05), IFH (23.87 ± 3.05 mm vs 21.41 ± 2.95 mm, P < 0.05), and IFA (212.14 ± 51.82 mm2 vs 177.07 ± 51.73 mm2, P < 0.05) after surgery were significantly increased in the OLIF group. The ASC was increased significantly after the operation in both groups, but the ASC in the MISTLIF group was increased significantly more than that in the OLIF group (450.04 ± 66.66 mm2 vs 171.41 ± 58.55 mm2, P < 0.05). The lumbar VAS scores at 1 month (1.89 ± 0.87 vs 2.34 ± 0.84, P = 0.028) and 6 months (1.23 ± 0.97 vs 1.80 ± 0.99, P = 0.018) after operation in the OLIF group were significantly lower. There were no significant differences in lower extremity VAS and ODI scores between the two groups. Compared with MISTLIF group, HADS scores on postoperative day 3 (2.91 ± 1.46 vs 4.89 ± 1.78, P < 0.05) and prior to hospital discharge (PTD) (2.54 ± 1.38 vs 3.80 ± 1.78, P = 0.002) in the OLIF group were decreased significantly. OLIF showed more advantages of less surgical invasion, lower incidence of postoperative low back pain, faster postoperative recovery, and less anxiety compared with MISTLIF. Regardless of cost, OLIF seems to be a better option to treat mild to moderate symptomatic DLSS.

Critical hydraulic gradient and fine particle migration of sand under upward seepage flow.

In order to study the mechanism of seepage-induced geotechnical damage and characteristics of fine particle migration in sandy soil, a one-dimensional upward seepage test device was used and a series of upward seepage flow tests of sand were conducted. In these experiments, the permeability, fine particle migration and redistribution under different hydraulic gradients were investigated. The results show that local hydraulic gradient in the sand sample is larger than the critical hydraulic gradient calculated by the Terzaghi's Equation. The seepage force will induce the fine soil particles to migrate along water flow direction and then cause the redistribution and reconsolidation of sand particles. Under the test condition, fine particles (< 0.075 mm) and fine sand particles (0.075-0.25 mm) dominate in the lost soil particles. Soil particles larger than 0.6 mm are hardly to lose.

Comparison of Percutaneous Transforaminal Endoscopic Discectomy and Microscope-Assisted Tubular Discectomy for Lumbar Disc Herniation.

OBJECTIVE: The aim of the present study was to compare the clinical outcomes and quality of life following percutaneous transforaminal endoscopic discectomy (PTED) and microscope-assisted tubular discectomy (MTD) for lumbar disc herniation (LDH). METHODS: This study had a retrospective design. From June 2017 to June 2018, the clinical data of 120 patients with LDH treated with PTED (60 cases, PTED group) and MTD (60 cases, MTD group) were analyzed and followed up for at least 20 months. There were 59 men and 61 women. Patients were aged between 22 and 80 years. The operation time, intraoperative blood loss, incision length, frequency of intraoperative fluoroscopy, cost, hospital stay, types of herniated discs, complications, and clinical outcomes were evaluated. Clinical outcomes were assessed using the visual analog scale (VAS), the Oswestry disability index (ODI), and the modified Macnab criteria. Short-Form 36 (SF-36) and the EQ-5D-5L were used to evaluate the quality of life of patients. The data between the two groups were compared by independent sample t-tests. Multiple comparisons between samples were analyzed by analysis of variance. RESULTS: Compared with the MTD group, the PTED group had shorter incision length (9.20 ± 1.19 mm vs 26.38 ± 1.82 mm), less intraoperative blood loss (18.00 ± 4.97 mL vs 39.83 ± 6.51 mL), and shorter hospital stay (5.42 ± 5.08 days vs 10.58 ± 3.69 days) (P = 0.00). PTED was much more appropriate for foraminal and extraforaminal disc herniation. The incidence of paresthesia was lower in the PTED group (6.67% vs 16.67%). At each follow up, the VAS and ODI scores of all patients were significantly improved compared with those before surgery (P = 0.00). At 3 days postoperatively, the lumbar VAS score of the PTED group was significantly lower (1.58 ± 1.00 vs 2.37 ± 1.10, P = 0.00). The excellent rate of the PTED group reached 91.67%, and that of the MTD group reached 93.33%. Compared with the preoperative SF-36 scores for physiological function, mental health, and social function, the postoperative scores were significantly improved in both groups (P = 0.00). The EQ-5D-5L in the PTED group increased from 0.30 ± 0.17 before the operation to 0.69 ± 0.13 after 6 months of follow up (P = 0.00) and 0.73 ± 0.14 after 20 months of follow up. The EQ-5D-5L in the MTD group increased from 0.28 ± 0.17 before the operation to 0.68 ± 0.13 after a 6-month follow up (P = 0.00), and 0.73 ± 0.12 after a 20-month follow up. CONCLUSION: Although both PTED and MTD are effective for LDH, PTED is much more appropriate for various types of LDH and has the advantages of the low incidence of low back pain, fewer complications, and early recovery.

Clusterin inhibits Aß42 aggregation through a "strawberry model" as detected by FRET-FCS.

Extracellular plaque deposits of ß-amyloid peptide (Aß) are one of the main pathological features of Alzheimer's disease (AD). The aggregation of Aß42 species, especially Aß42 oligomers, is still an active research field in AD pathogenesis. Secretory clusterin protein (sCLU), an extracellular chaperone, plays an important role in AD pathogenesis. Although sCLU interacts directly with Aß42 in vitro and in vivo, the mechanism is not clear. In this paper, His-tagged sCLU (sCLU-His) was cloned, expressed and purified, and we applied florescence resonance energy transfer-fluorescence correlation spectroscopy (FRET-FCS) to investigate the direct interaction of sCLU-His and Aß42 at the single-molecule fluorescence level in vitro. Here, we chose four different fluorescently labeled Aß42 oligomers to form two different groups of aggregation models, easy or difficult to aggregate. The results showed that sCLU-His could form complexes with both aggregation models, and sCLU-His inhibited the aggregation of Aß42/RB  ~ Aß42/Atto647 (easy to aggregate model). The complexes were produced as the Aß42/Label adhered to the sCLU-His, which is similar to a "strawberry model," as strawberry seeds are dotted on the outer surface of strawberries. This work provided additional insight into the interaction mechanism of sCLU and Aß42 .

Built environment, physical activity, and obesity of adults in Pingshan District, Shenzhen City in Southern China.

BACKGROUND: The relation between neighbourhood built environment and obesity has been described as both nuanced and complex. AIM: The objective of this study was to examine the relationship between the built environment, physical activity, and obesity in a rapidly urbanised area of China. SUBJECTS AND METHODS: This is a cross-sectional study. Descriptive statistics were used to describe the socio-demographic variables, physical activity levels and BMI status. Multivariable logistic regression models were used to examine the association between neighbourhood environment, the likelihood of engaging in different types of physical activity, and BMI. RESULTS: A total of 842 respondents completed the questionnaires and were included (84.1% response rate). Among them, 56.4% reported meeting high physical activity levels, while 40.7% were overweight or obese. Multivariable regression analysis showed that better road conditions (ß = 0.122, t = 2.999, p = 0.003) and access to physical activity facilities (ß = 0.121, t = 3.193, p = 0.001) were significantly associated with higher levels of physical activity. Physical activity levels were inversely associated with the likelihood of being overweight (OR = 0.565, 95%CI: 0.3 4 9-0.917) or obese (OR = 0.614, 95%CI: 0.3 9 0-0.966). CONCLUSION: The built environment has an important impact on physical activity. However, the direct impact of leisure physical activity on BMI is not significant. This research provides a summary of recent evidence in Pingshan District on built environments that are most favourable for physical activity and obesity.

Neighbourhood built environment and leisure-time physical activity: A cross-sectional study in southern China.

This study conducted scientific evidence linking neighbourhood built environment to adults' leisure-time physical activity (LTPA) among adults in China. Data were obtained from a questionnaire survey conducted from April to July 2017 among 1002 adults aged 18-69 years old in Pingshan District, Shenzhen, China. Chinese Walkable Environment Scale for urban community residents and International Physical Activity Questionnaire were used to measure participants' neighbourhood built environment and leisure-time physical activity, which was categorised into leisure-time walking (LTW) and leisure-time moderate-to-vigorous physical activity (LTMVPA). A total of 986 participants (mean age = 40.7 years, 53.3% females) were included in this research. Descriptive statistics were used to describe the socio-demographic variables, LTW and LTMVPA by sex. Multivariable logistic regression models were used to examine the association between neighbourhood environment characteristics and the likelihood of engaging in active LTW and LTMVPA. Only 20.7% of participants engaged in active LTW and 17.8% active LTMVPA. Better road condition was associated with higher likelihood of active (at least 150 min/week) LTW and LTMVPA. High perceived esthetic was positively associated with LTW and LTMVPA. Active LTW was related to better perception of traffic condition as well. The improvement of the neighbourhood environment characteristics can promote active LTPA among adults living in Shenzhen, China. Our findings support the importance of considering population health effects in urban planning and development.

Investigation on the Structure and Fractal Characteristics of Nanopores in High-Rank Coal: Implications for the Methane Adsorption Capacity.

The structure and fractal characteristics of nanopores of high-rank coal were investigated using an approach that integrates N2 adsorption and field emission scanning electron microscopy (FE-SEM). The results indicated that the high-rank coal of the Shanxi Formation has a complex pore-fracture network composed of organic matter pores, mineral-related pores, and microfractures. The pore type of high-rank coal tends to be complicated, and the main pore types are inkbottle pores and open pores, which are more conducive to methane enrichment. The Ro,max has a negative relationship with the total pore volume. In addition, the ash and inertinite contents show a positive correlation with the average pore size (APS), while the fixed carbon content exhibits a negative relationship with the APS. The pore structure of high-rank coal is controlled not only by the degree of metamorphism but also by coal composition, which leads to the variation in pore structure becoming more complicated. With the increase in coal metamorphism, high-rank coal with high amounts of fixed carbon content generally possesses a higher irregularity in pore structure. No obvious relationship was observed between D2 and the coal components, which indicates that the pore structure, ash content, moisture content and other factors controlled by the metamorphism of coal have different effects on D2 that lead to this inapparent relationship. A negative relationship exists between adsorption volume and D1, which indicates that the high irregularity of the pore structure is not conducive to methane absorption and that no obvious correlation exists between the adsorption volume and D2. In the high-rank coal, the high D1 value represents the complexity and heterogeneity of the pore structure and represents a low adsorption affinity for methane molecules; in addition, D2 has no effect on the methane adsorption capacity.

Characteristics of an electron beam extracted from a microwave electron cyclotron resonance plasma cathode.

Under ultra-low pressure, an electron beam is generated by the microwave electron cyclotron resonance plasma with a permanent magnet. By means of the Hall coil and Miller ampere meter, two electron current signals at the end of the extraction anode (Ia) and the sample holder (Is) were measured. The influences of discharge power, gas pressure, and accelerating voltage on the electron beam current Is were analyzed. The experimental results indicated that the extracted electron current can reach 75.1 mA at a gas pressure of 1.4 × 10-3 Pa, an accelerating voltage of 8 kV, and an orifice size of 3 mm in diameter. In order to estimate the focusing properties of the electron beam by the beam-focusing coil, the influence of the coil current on the size of the electron beam spot on the sample holder was presented and discussed. The intrinsic mechanism of electron beam extraction and propagation is revealed by numerical simulation.

Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells.

PURPOSE: This study aimed to investigate the potential mechanism and value of lupeol in inhibiting high-glucose-induced apoptosis in rabbit nucleus pulposus cells (NPCs). METHODS: NPCs were divided into four groups: control (CON), high glucose (HG), LUP, and HG + LUP. Viability, reactive oxygen species (ROS) levels, and apoptosis were examined in NPCs. The protein expression levels of Bax, Bcl-2, cytochrome C, and caspase 9/3 were measured using reverse transcription-polymerase chain reaction and Western blot assay. RESULTS: The apoptotic rate and total ROS level of the HG group significantly increased compared with the CON group (P < 0.01). The total ROS level in the HG + LUP group significantly decreased compared with the HG group(P < 0.05). The mRNA expression of Bcl-2 was significantly upregulated, whereas the expression of Bax, cytochrome C, and caspase 9/3 was downregulated in the HG + LUP group compared with those in the HG group(P < 0.05).The Western blot assay showed that the expression of Bcl-2 was upregulated, but the expression of Bax, cytochrome C, and caspase 9/3 was significantly downregulated in the HG + LUP group compared with the HG group (P < 0.05). CONCLUSIONS: Lupeol inhibited high-glucose-induced apoptosis in NPCs by enhancing the anti-oxidative stress in the mitochondria. This study suggested lupeol as a potential therapeutic drug for treating intervertebral disc degeneration under hyperglycaemic conditions. These slides can be retrieved under Electronic Supplementary Material.

Event-related desynchronization and synchronization quantification in motor-related EEG by Kolmogorov entropy.

OBJECTIVE: Various approaches have been applied for the quantification of event-related desynchronization/synchronization (ERD/ERS) in EEG/MEG data analysis, but most of them are based on band power analysis. In this paper, we sought a novel method using a nonlinear measurement to quantify the ERD/ERS time course of motor-related EEG. APPROACH: We applied Kolmogorov entropy to quantify the ERD/ERS time course of motor-related EEG in relation to hand movement imagination and execution for the first time. To further test the validity of the Kolmogorov entropy measure, we tested it on five human subjects for feature extraction to classify the left and right hand motor tasks. MAIN RESULTS: The results show that the relative increase and decrease of Kolmogorov entropy indicates the ERD and ERS respectively. An average classification accuracy of 87.3% was obtained for five subjects. SIGNIFICANCE: The results prove that Kolmogorov entropy can effectively quantify the dynamic process of event-related EEG, and it also provides a novel method of classifying motor imagery tasks from scalp EEG by Kolmogorov entropy measurement with promising classification accuracy.

Quantitative analysis of gait coordination based on gait events in children with cerebral palsy.

OBJECTIVE: The objective of this study was to quantify gait coordination of the lower limbs in children with cerebral palsy (CP) based on gait events. DESIGN: The kinematic data of 50 children with typical development and 26 children with CP prerehabilitation and postrehabilitation were recorded. The hip and knee joint angles in the sagittal plane on both sides were obtained at six gait events. Then a gait coordination index (GCI) model was established based on the gait features extracted from the joint angles of the children with typical development using kernel-based principal component analysis, which was then used to calculate the GCI of children with CP. One-way analysis of variance was used to compare GCI and joint angles at each gait event. Intraclass correlation coefficient was calculated to evaluate the reliability of GCI in two trials separated by a day. RESULTS: GCI in children with CP postrehabilitation was significantly higher than that in the children with typical development (P < 0.05) but significantly lower than that in children with CP prerehabilitation (P < 0.05). There are significant differences in GCI for children with CP prerehabilitation between level I, level II, and level III (P < 0.05). The results of intraclass correlation coefficients (>0.8) indicated that the obtained GCIs were reliable. CONCLUSIONS: GCI can reflect gait coordination of lower limbs in children with CP and may be a useful tool for rehabilitation assessment.

Destruction of inorganic municipal solid waste incinerator fly ash in a DC arc plasma furnace.

Due to the toxicity of dioxins, furans and heavy metals, there is a growing environmental concern on municipal solid waste incinerator (MSWI) fly ash in China. The purpose of this study is directed towards the volume-reduction of fly ash without any additive by thermal plasma and recycling of vitrified slag. This process uses extremely high-temperature in an oxygen-starved environment to completely decompose complex waste into very simple molecules. For developing the proper plasma processes to treat MSWI fly ash, a new crucible-type plasma furnace was built. The melting process metamorphosed fly ash to granulated slag that was less than 1/3 of the volume of the fly ash, and about 64% of the weight of the fly ash. The safety of the vitrified slag was tested. The properties of the slag were affected by the differences in the cooling methods. Water-cooled and composite-cooled slag showed more excellent resistance against the leaching of heavy metals and can be utilized as building material without toxicity problems.