Evaluating micro-nano bubbles coupled with rice-crayfish co-culture systems: A field study promoting sustainable rice production intensification.

Traditional rice-fish symbiosis systems efficiently use soil and water resources but the adverse effects of prolonged flooding on the stability of rice growth can be mitigated. The feasibility and efficacy of injecting micro-nano bubbles (MNBs) in rice-crayfish co-cultures was investigated in a 22-hectare field experiment conducted over five months. This injection significantly enhanced the growth of both rice and crayfish, and increased total nitrogen and phosphorus levels in the soil, thereby augmenting fertility. Analysis of dissolved oxygen (DO), water temperature and gene expression (rice and crayfish) clarified that micro-nano bubbles (MNBs) foster an optimal environment for rice root respiration, whereas rice establishes an optimal temperature for crayfish, thereby enhancing their activity and growth. Comparative analyses of gene expression profiles and metabolic pathway enrichment revealed that the injection of MNBs diversifies soil microbial communities and intensifies biological processes, such as plant hormone signal transduction. This was in marked contrast to the situation in our controls, rice monoculture (R) and micro-nano bubbles rice monoculture (MNB-R). The combination of rice-fish symbiosis with MNBs led to a 26.8 % increase in rice production and to an estimated 35 % improvement in economic efficiency. Overall, this research introduces an innovative and environmentally sustainable method to boost rice yields, thereby enhancing food security and providing additional income for farmers.

Effect of micro-nano bubbles on the remediation of saline-alkali soil with microbial agent.

The widespread distribution of saline-alkali soil around the world affects the health of ecological systems and the development of the national economy by limiting the growth of plants. However, the commonly used remediation technologies have the drawbacks of low efficiency, high cost, and secondary pollution. This study investigated the feasibility and efficacy of novel combined micro-nanobubbles (MNBs) and microbial agent (MA) technology for the remediation of saline-alkali soil. The results demonstrated that the combined MA-MNBs method greatly renovated the properties of saline-alkali soil compared with the technologies of single utilization of MA or MNBs process in the laboratory. The method resulted in a reduction of soil electrical conductivity and pH levels, an improvement in soil fertility, and the formation of soil aggregates. Moreover, the method significantly impacted the growth of plants, particularly in plant length, dry weight, and rhizome elongation. Further high-throughput sequencing and gene expression analysis revealed that the MA-MNBs method enhanced the abundance of soil microbial community compared with single MA and MNBs treatment. Gene enrichment analysis revealed that the MA-MNBs method could compensate for the shortcomings of single MA treatment and enhance the expression of energy metabolism and salt stress-related genes attributed to MNBs treatment, thereby significantly improving the growth and development of plants. Consistently, 6115 kg/ha of rice was yielded in the field for the saline-alkali soils using this MA-MNBs method, with zero crops before remediation. This study provided a novel, efficient, and green strategy for the remediation of saline-alkali soil without adding any chemicals.

Simultaneous remediation of arsenic and organic chemicals contaminated soil and groundwater using chemical oxidation and precipitation/stabilization: a case study.

After the departure of industrial facilities, reuse of the land in developed cities in China is problematic, due to the land contamination issues. The rapid remediation of sites with complex contamination is crucial and urgently needed. Herein, the case of on-site remediation of arsenic (As) in soil, as well as benzo(a)pyrene, total petroleum hydrocarbons, and As in groundwater was reported. For contaminated soil, the oxidant and deactivator (consisting of 20% sodium persulfate, 40% ferrous sulfate (FeSO4), and 40% portland cement) were applied to oxidize and immobilize As. As a result, the total amount and lixivium concentration of As were constrained under 20 mg/kg and 0.01 mg/L, respectively. Meanwhile, for contaminated groundwater, As and organic contaminants were treated by FeSO4/ozone and FeSO4/hydrogen peroxide with mass ratios of 1:5 and 1:8, respectively. The continuous monitoring of contaminants in 22 monitoring wells shown that all contaminants in groundwater were treated to meet the standards. In addition, the risk of secondary pollution and operation cost was effectively reduced by proper disposal and resourceful utilization. The findings indicated that the method of oxidation and precipitation/stabilization is technically, environmentally, and economically feasible for the remediation of contaminated sites with similar complex pollutants.