Intrathecal granulocyte colony-stimulating factor modulate glial cell line-derived neurotrophic factor and vascular endothelial growth factor A expression in glial cells after experimental spinal cord ischemia.

The hematopoietic growth factor, granulocyte colony-stimulating factor (G-CSF), has become one of the few growth factors approved for clinical use. It has therapeutic potential for numerous neurodegenerative diseases; however, at present the cellular effects of G-CSF on the central nervous system remain unclear and in need of investigation. In the present study, we used spinal cord ischemia, a neurodegenerative model, to examine the effects of intrathecal (i.t.) G-CSF on glial cell (microglia and astrocyte) activation and neuroprotective factor expression, including glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor A (VEGF-A) protein expression. Our results indicate that i.t. G-CSF could enhance ischemia-induced microglial activation and inhibit ischemia-induced astrocyte activation. Both GDNF and VEGF-A are upregulated after injury, and i.t. G-CSF could enhance GDNF and VEGF-A expressions after injury. Interestingly, our results indicate that performing i.t. G-CSF alone on normal animals could have the effect of microglial and astrocyte activation and enhanced GDNF and VEGF-A expressions. Furthermore, through laser scanning confocal microscopy, we found that astrocytes may contribute to the majority of GDNF and VEGF-A expressions of G-CSF after spinal cord ischemia. Overall, this G-CSF-induced upregulation suggests that activation of endogenous neuroprotective mechanisms could resist neurodegenerative insults. These observations demonstrate the cellular mechanism of i.t. G-CSF after spinal cord ischemia and confirm the neuroprotective effect of G-CSF after spinal cord ischemia injury.

Reduction of ordering temperature of self-assembled FePt nanoparticles by addition of Au and Ag.

The [FePt]94Au6 and [FePt]90Ag10 nanoparticle arrays were synthesized on Si substrates by a reverse micellar method, combined with plasma treatment and in-situ deposition of a SiO2 overlayer, and the post annealing step was performed to drive the face-centered cubic to tetragonal phase transition. These FePt nanoparticles exhibit a quasi-hexagonal order with tailored inter-particle spacing and particle size. The effects of the Ag and Au on the structural and magnetic properties of FePt were investigated. The results indicate that both Au and Ag additives can remarkably enhance the coercivity and reduce the ordering temperature, however, the optimum composition is different for them. The optimum composition is determined to be [FePt]94Au6 and [FePt]90Ag10, respectively, for which the ordering temperature of FePt nanoparticles is reduced by -100 degrees C. After 600 degrees C annealing, the [FePt]94Au6 and [FePt]90Ag10 nanoparticles are totally ferromagnetic with apparent larger coercivities of -7.0 kOe, which is about 3.8 kOe larger than that of the pure FePt nanoparticles. The mechanism of the chemical ordering acceleration may be attributed to the defects and strains caused by the Au/Ag additives.

Localized-Surface-Plasmon Enhanced the 357 nm Forward Emission from ZnMgO Films Capped by Pt Nanoparticles.

The Pt nanoparticles (NPs), which posses the wider tunable localized-surface-plasmon (LSP) energy varying from deep ultraviolet to visible region depending on their morphology, were prepared by annealing Pt thin films with different initial mass-thicknesses. A sixfold enhancement of the 357 nm forward emission of ZnMgO was observed after capping with Pt NPs, which is due to the resonance coupling between the LSP of Pt NPs and the band-gap emission of ZnMgO. The other factors affecting the ultraviolet emission of ZnMgO, such as emission from Pt itself and light multi-scattering at the interface, were also discussed. These results indicate that Pt NPs can be used to enhance the ultraviolet emission through the LSP coupling for various wide band-gap semiconductors.

Composition deviation of arrays of FePt nanoparticles starting from poly(styrene)-poly(4-vinylpyridine) micelles.

Ordered arrays of FePt nanoparticles were prepared using a diblock polymer micellar method combined with plasma treatment. Rutherford backscattering spectroscopy analyses reveal that the molar ratios of Fe to Pt in metal-salt-loaded micelles deviate from those when metal precursors are added, and that the plasma treatment processes have little influence upon the compositions of the resulting FePt nanoparticles. The results from Fourier transform infrared spectroscopy show that the maximum loadings of FeCl(3) and H(2)PtCl(6) inside poly(styrene)-poly(4-vinylpyridine) micelles are different. The composition deviation of FePt nanoparticles is attributed to the fact that one FeCl(3) molecule coordinates with a single 4-vinylpyridine (4VP) unit, while two neighboring and uncomplexed 4VP units are required for one H(2)PtCl(6) molecule. Additionally, we demonstrate that the center-to-center distances of the neighboring FePt nanoparticles can also be tuned by varying the drawing velocity.

Cerebral intraventricular hemorrhage caused by a large cerebral arteriovenous malformation at 31 years after diagnosis.

Cerebral arteriovenous malformation (AVM) has an overall 2% to 4% annual risk of hemorrhage. The annual risk of hemorrhage does not decrease with age. However, the natural history of non-operative AVM is difficult to follow up consistently for more than 30 years. In this report, we present a case with a large cerebral AVM which developed a major bleeding causing intraventricular hemorrhage (IVH) 31 years after the diagnosis. A male patient was proved to have a cerebral AVM in the right corpus callosum by cerebral angiography at ages 20 and 28. He was found losing consciousness due to rupture of AVM with IVH at age 51. A small cerebral aneurysm and a dilated vein (aneurysm) of Galen were noted also. A craniotomy with removal of the hematoma and microsurgical resection of the AVM was performed immediately, and the patient regained normal respiration and consciousness about 2 months and 7 months after craniotomy, respectively. We recommend that neurosurgeons should play active roles in encouraging young patients with large AVMs to receive microsurgical resection.